, Kaoru ARIYAMA 10 17N, Na, Mg,Al,Si,P,S,Cl,K,Ca,Fe,Cu,Zn,Se,Br,Rb Sr 14r2 0.9617 Tricholoma matsutake,,, JAS ICP-OESICP-MS 2ICP-OES ICP-MS,, Tricholoma matsutake -24-
1 7 2006 70 13 8 5 2,, 2007,, 10 17,,,,,,, 14 44, 22, 22, 28, 15, 5 25 g ICP-OES ICP-MS 2-25-
Ariyama, SRMNational Institute of Standard & Technology Gaithersburg, MD, USA Wheat FlourSRM1567aRice FlourSRM1568aSpinach Leaves SRM1570aApple LeavesSRM1515Tomato LeavesSRM1573aPine NeedlesSRM1575a Corn KernelRM8413China National Analysis Center for Iron and SteelBeijing, China TeaGBW07605CabbageGBW08504Bush Branches and LeavesGBW0760310-26-
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B-400 FC-610 MP-35-02 XRF-17500 MilliQ Element 18 MΩcm 1cm 56 g 105 1216 1.53.5 g 35 mm 30 t 20 5mm 30 mm TiN, Na, Mg, Al, Si, P, S, Cl, K, Ca, Fe, Cu, Zn, Se, Br, Rb Sr 17 30 SRM Bush Branches and Leaves BG 2θ a n b r 2 (s) (s) (deg) N 30 15 Kα 33.830 c raw 7 0.9184 0.58 0.46 % Na 40 20 Kα 55.080 raw 6 0.9576 32 10 µg/g Mg 10 5 Kα 45.100 0 Net 10 0.9886 0.027 0.046 % Al 30 15 Kα 145.180 raw 8 0.9849 75 117 µg/g Si 20 10 Kα 109.040 0 Net 2 0.9822 0.019 0.042 % P 10 5 Kα 140.880 raw 8 0.9307 0.20 0.014 % S 10 5 Kα 110.540 0 Net/BG 7 0.9717 0.034 0.053 % Cl 30 15 Al Kα 92.680 raw 6 1.0000 0.018 0.0040 % K 10 5 Kα 136.690 raw 8 0.9815 1.0 0.13 % Ca 10 5 Kα 113.090 0 Net 9 0.9921 0.0044 0.14 % Fe 20 10 Kα 57.520 Net/BG 8 0.9897 44 37 µg/g Cu 40 20 Kα 45.030 Net/BG 10 0.9923 3.2 0.44 µg/g Zn 30 15 Kα 41.800 Net/BG 9 0.9915 6.4 2.0 µg/g Se 40 20 Kα 31.890 0 Net 9 0.9659 0.31 0.12 µg/g Br 40 20 Kα 29.970 Net/BG 5 0.9925 0.30 0.72 µg/g Rb 20 10 Kα 26.620 Net 10 0.9868 2.3 35 µg/g Sr 40 20 Kα 25.150 0 Net/BG 5 0.9868 0.69 2.6 µg/g a BG1 BG2 b c raw d 0 e Net -29-
STATISTICA Pro 06J Micrisoft Excel 2002Micrisoft corporation, Redmond, WA, USA Welch t Micrisoft Excel 2002 10-fold cross validation 10 10 10 10 raw NetNet Net BG SRM NetNet BG 0 r Net BG r Net BG Fe 3 r Net BG Net r NetBG Na Fe 3 r Net BG r raw SRM SRM N S 3 r r N Net S Net BG Mg rawnet r Net BG r Net 0 P Net BG r raw Net BG r BG BG1 BG2 BG -30-
Ca Net BG raw Net r Net 0 Cl K raw Net BG BG1 BG2 raw BG raw BG1BG2 17 N, Na P r 2 0.96 7 SRM Net raw BG1 BG BG2 (a) raw (b) Net (c) Net /BG Fe 3 (a-1) raw (a-2) raw (b) Net (c) Net / BG Na 3-31-
(a) raw (b-1) Net (b-1) Net (c) Net / BG Mg 3 (a) raw (b-1) Net (b-1) Net (c) Net / BG Ca 3 SRM Bush Branches and Leaves 7 repeatability 5 reproducibility Se repeatability 7 reproducibility 9 SRM Bush Branches and Leaves repeatability reproducibility repeatability reproducibility (%) (%) N 1.5 % 1.24 0.25 5.2 1.20 0.27 5.0 Na 1.96 % 2.02 926 4.6 1.75 891 6.3 Mg 0.48 % 0.59 0.03 4.7 0.51 0.025 4.8 Al 2000 µg/g 1960 33 1.7 1810 126 7.0 Si 0.60 % 0.69 0.03 3.7 0.64 0.06 9.2 P 0.100 % 0.096 0.005 5.2 0.081 0.005 6.3 S 0.73 % 0.71 0.02 2.3 0.67 0.02 2.4 Cl 1.92 % 2.42 0.09 3.8 1.97 0.04 2.0 K 0.92 % 0.88 0.03 3.3 0.81 0.03 3.4 Ca 1.68 % 2.06 0.05 2.4 1.92 0.06 3.4 Fe 1070 µg/g 1120 25 2.2 1070 18 1.6 Cu 6.6 µg/g 6.6 0.3 4.5 6.5 0.3 4.3 Zn 55 µg/g 62.2 1.3 2.1 58.9 1.7 2.8 Se 0.12 µg/g -0.091 0.018-20.2 0.06 0.09 142.2 Br 3.0 µg/g 3.70 0.17 4.6 3.53 0.15 4.3 Rb 4.5 µg/g 3.48 0.23 6.6 3.26 0.27 8.5 Sr 246 µg/g 264 3 1.2 247 11 4.4-32-
ICP-OES ICP-MS 2 Mg P Mg SRM P SRM 0.100.518 ICP-OES ICP-MS,, 10 Na Mg Na Mg P SRM Fundamental Parameter FP ICP ICP ICP N 2.1 3.0 % Na 94 87 111 98 µg/g Mg 0.077 0.117 0.090 0.126 % Al 85 67 139 128 µg/g Si ND 0.037 % P 0.48 0.610 0.65 0.728 % S 0.36 0.49 % Cl 0.09 0.09 % K 2.4 2.58 3.0 3.12 % Ca 0.016 0.019 0.008 0.011 % Fe 66 57 72 59 µg/g Cu 8 8.4 9 10.2 µg/g Zn 72 79.3 64 66.9 µg/g Se ND ND µg/g Br 0.5 0.7 µg/g Rb 32 36 46 58 µg/g Sr 0.8 0.68 ND 0.68 µg/g -33-
10 ICP ICP ICP ICP ICP N 1.4 0.7 ND 0.5 % Na 96 111 82 101 72 77 162 241 µg/g Mg 0.084 0.100 0.070 0.088 0.068 0.084 0.118 0.163 % Al ND ND ND ND µg/g Si ND ND ND ND % P 0.263 0.300 0.224 0.284 0.255 0.298 0.231 0.293 % S 0.33 0.24 0.21 0.30 % Cl 0.08 0.10 0.09 0.10 % K 1.8 1.5 1.7 1.5 % Ca 0.190 0.157 0.126 0.137 % Fe ND ND ND ND µg/g Cu ND 3 ND 5 µg/g Zn 17 17.6 13 13.9 12 13.0 5 7.1 µg/g Se ND ND ND ND µg/g Br 7 1.9 2.9 1.1 µg/g Rb 9.5 9.0 1.6 1.2 2.7 2.6 5.5 5.5 µg/g Sr 5.1 7.6 4.5 6.1 1.8 3.6 17.0 21.0 µg/g 17 11 4 1 11 n=44 n=22 n=22 n=28 n=15 n=4 Mean SD Mean SD Mean SD Mean SD Mean SD Mean SD N 2.60 0.78 2.89 0.50 3.26 0.48 ** 2.95 0.59 2.95 0.39 2.71 0.42 % Na 99 179 64 27 142 234 105 40 68 33 209 70 µg/g Mg 0.052 0.010 0.053 0.012 0.054 0.008 0.060 0.010 ** 0.070 0.008 ** 0.069 0.013 % Al 100 117 230 323 180 114 213 123 ** 267 121 ** 140 112 µg/g Si 0.028 0.020 0.081 0.100 0.048 0.029 ** 0.069 0.044 ** 0.101 0.048 ** 0.078 0.041 % P 0.310 0.090 0.336 0.092 0.404 0.074 ** 0.437 0.090 ** 0.487 0.080 ** 0.366 0.062 % S 0.180 0.035 0.203 0.039 0.193 0.036 0.207 0.042 ** 0.207 0.036 0.230 0.030 % Cl 0.07 0.04 0.05 0.03 0.05 0.03 0.04 0.02 ND ** 0.05 0.01 % K 3.44 0.48 3.69 0.48 3.58 0.41 3.92 0.52 ** 4.20 0.688 3.62 0.03 % Ca 0.008 0.004 0.013 0.006 ** 0.013 0.007 0.022 0.009 ** 0.040 0.014 ** 0.014 0.006 % Fe 154 68 233 122 ** 217 75 ** 272 168 ** 357 115 ** 232 59 µg/g Cu 24 7 20 8 18 5 ** 26 14 19 6 ** 19 4 µg/g Zn 70 20 76 16 73 24 79 21 53 10 ** 111 22 µg/g Se 7.8 4.8 5.4 2.3 ** 4.8 1.9 ** 4.7 4.4 ** 5.2 2.1 4.8 2.0 µg/g Br 1.3 1.3 0.3 0.5 0.8 0.6 ND ** ND ** 1.8 0.7 µg/g Rb 210 138 196 138 175 120 151 133 87 38 ** 264 67 µg/g Sr ND 0.2 0.7 ND 0.7 1.1 ND 0.3 0.5 µg/g ** 1% -34-
,,, 4 4Mg, Si, P, K, Ca Cu 4582 12 4,, Mg, Si, P, K, Ca Cu 93 90 10-fold cross validation 89 82 2007 10-fold cross validation 7786 12, 64, 68 71 11 4 n 43 82 36 2 5 1 22 45 5 10 5 2 22 73 2 3 16 1 28 64 0 2 8 18 12 crossvalidation crossvalidation Al, Si, P, K, Zn 82 77 Na,P,K,Ca,Se,Rb 82 86 N, K, Ca 86 79-35-
5g 0.3 g 2 1910 FP 10 17N, Na, Mg,Al,Si,P,S,Cl,K,Ca,Fe,Cu,Zn,Se,Br,Rb Sr 14 r2 0.96,, Mg, Si, P, K, Ca Cu 6 10-fold cross validation 89 82 1 53489-497 2006 2 Ariyama, K; et al, J. Agric. Food Chem., 55, 347-354 2007 3 2007 4 561053-10612007 -36-