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Transcription:

1080 Vol.21 No.9 2002 3 3, 1 2 1 3.1 1 AB A 3 2 1

Vol.21 No.8 No.9 2002 1081 872.7 1 9.1 218.2 11 : (1999) 27: 263-269 1 1 3 2 3 3 2 2.6 C 4 19

1082 Vol.21 No.9 2002 A B C 1 X-chrom lens 5 ChromaGen X-chrom 6 1A 1B 3 2 1AB 1 2 3

Vol.21 No.9 2002 1083 7 1 1 1C 1)2) 2 3 8 9 3 2 1 3 2 3 2 1 X1 2 ES 1

1084 Vol.21 No.9 2002 10 3.2 2 2.2 F 11 A B

Vol.21 No.9 2002 1085 A B 100 80 60 40 20 0 400 500 600 650 2 1 3 Photoshop 1 2 2A 3A 3B 2 2B 1 http://www.vischeck.com/daltonize/ 2 2.2 K 4http:// www.hokkei.co.jp/c_1.html 6mm LED RGBJIS Z8102 JIS 31

1086 Vol.21 No.9 2002 220 12 6mm Macintosh Xproof http://www.colorfield. com/xproof/ Windows http://www. hikarun.com/w/ RGB Xproof JIS NBS/ISCC HTML X11 http://www.mcfedries.com/books/ cightml/x11color.htm PDF Xproof RGB JIS NBS/ISCC 12 JIS JIS http://www. colordream.net/jiscolor1.htm 13 JIS RGB 100 3.3 300 2 14 3 2

Vol.21 No.9 2002 1087 15 16 3.4 DNA CCD 1 FITCGFP Rhodamine DsRed 2 1 1 5 2 3 16C 618 90 100 60 100

1088 Vol.21 No.9 2002 0 64 128 192 255 A B 0 10 20 30 40 50 60 70 80 90 100% C D figure legend 17 DNA 2 7A 7B 1 2 DNA 7C 18 2 7D CCD

Vol.21 No.9 2002 1089 Fluoview application software Ver.4.2 <LUT> Color Tool<Ch1> <Ch5> Standard Color LUTs AxioCam Axiovision LSM510 LSM5 Pascal 24 Configuration Control Ch- <Define> HLS RGB RGB 255,0,255 Select <Chan> Channels <Colors> RGB 255,0,255 <Add> EG-Lab C1EZ-C1 View settings 2 <File> <Save As> TIFF BMP <Method> <Edit> Methods "Double Green/Red" <Edit Method> Double Green/Red-Method wizard "Double Green/Magenta" <NEXT> 4 Look up Tables names "Magenta.lut" "Red.lut" Pane1 Pane3 "Red.lut" <NEXT><FINISH> Acquisition <Beam> <OK> CCD FW4000 <Setup> < > <OK> RGB 255, 0, 255 <OK> Look Up Table Look UpTable 19 2 20 TIFF PICTBMP RGB 8Photoshop 1 A C 3 V ^ Macintosh Windows CTRL 1AC3V^ Photoshop 2 50

1090 Vol.21 No.9 2002 1 3 V ^ A Shift ~ ver. 44.5 ~ ASCII C 1AC3V^ 0 ver. 3 R G B 3 2 3 7 3 4 5 2 9A 21 2 AB A C A BA C 2 2 2

Vol.21 No.9 2002 1091 2 9B 22 23 RGB CCD figure legend 1 3.5 schema 2 46 13 redundant 8256 24 8 Color-vision deficiencies create problems for many people. Don't create software that uses only color coding to convey important information. Color coding should always be redundant to other types of cues, such as text, position, or highlighting. p.25softwarefigure

1092 Vol.21 No.9 2002 Windows Macintosh 10 Macintosh OS 9 Windows OS X 25 A B

Vol.21 No.9 2002 1093 11 4 2 12 3 3 13 EU A B C

1094 Vol.21 No.9 2002 B 3.6M C B B C 14 4 GATC 2 10 A B 15 B C A B

Vol.21 No.9 2002 1095 A B 26 10203040 5060701014.12028.2 4056.480 22 3.6 C 2 2.6 16400nm 700nm 2 2.6 D DTP 33 16A 0 360 3 16A 2 2.6 E 3 CIE 1931 xy 16B 2 2.6 F, G 520 530 540 510 550 560 570 500 580 590 600 490 650 700nm 480 470 400 510 500 490 480 520 470 400 530 540 550 560 570 580 590 600 650 700nm

1096 Vol.21 No.9 2002 16C xy 2 192.6 I 16A 0 1 2 2.2 E 0RGB=10000 27 17 28 xy 2 19 29 2 4 2 2 30 16C 3 1 18 31 120 130 140 16A 120 RGB 0

Vol.21 No.9 2002 1097 10002 1 1 32 120 150160 17 33 243 219C 17 2 2.2H RGB=0, 0, 100 18 violet purplemagenta 34 300 C,M,Y,K R,G,B 0 10 20 30 40 50 60 70 80 90 100 0 20 (0,100,100,0) (100,0,0) 40 60 80 100 120 140 160 (0,0,100,0) (100,100,0) (100,0,100,0) (0,100,0) 180 200 (100,0,0,0) (0,100,100) 220 240 260 280 (100,100,0,0) (0,0,100) 300 (0,100,0,0) (100,0,100) 320 340 24 19A 23

1098 Vol.21 No.9 2002 3 4 C,M,Y,K (%) R,G,B (0-255) R,G,B (%) (0,0,0,100) (0,50,100,0) (80,0,0,0) (0,0,0) (230,159,0) (86,180,233) (0,0,0) (90,60,0) (35,70,90) (97,0,75,0) (0,158,115) (0,60,50) (10,5,90,0) (100,50,0,0) (240,228,66) (0,114,178) (95,90,25) (0,45,70) (0,80,100,0) (213,94,0) (80,40,0) 20AB (10,70,0,0) (204,121,167) (80,60,70) 2 3 4 20C 19 3 3 5 5 35 1 2 3 19B 19C 19D 17 13B 20C 13C 20D

Vol.21 No.9 2002 1099 http://sun.endless.ne.jp/users/tanafic/ colorfiction/ank/ank_fr.htm? http:// www.microsoft.com/ japan/msdn/columns/hess/ hess10092000.asp 3.6 B 1 26 A A Times 3.7 PowerPoint ArialHelvetica 1 1 2 6 PowerPoint 3.8 3.4F

1100 Vol.21 No.9 2002 201 1 635nm 650nm670nm 21 36 1 635nm 1 650nm 670nm 532nm 21 37 1.0 0.8 0.6 0.4 0.2 0 532 400 500 600 700 nm 650 635 670 38 PowerPoint OHP OHP Association for Research in Vision and Ophthalmology ARVO J. Neitz M. Neitz 39 http://www.mcw.edu/cellbio/colorvision/excuse me.. Society for NeuroscienceAvoid using red and green next to

Vol.21 No.9 2002 1101 each other since many are red/green color blind. http://apu.sfn.org/images/am2002/prelimprog.pdf p.72 3.9 201 401 40 41 15 3 3.3 http://www. tenjin-chalk.co.jp/eye.htm 42 OHP

1102 Vol.21 No.9 2002 3 3 22 19 AB OAB 20 21 A A A A A A

Vol.21 No.9 2002 1103

1104 Vol.21 No.9 2002 A A A A A A A A A A A A 20 21 Medical College of Wisconsin Jay Neitz 1) (1962) 13: 611-616 2) (1975) 29: 119-125 3) Okajima K, et al: 9th Congress of the International Colour Association, Proceedings of SPIE (2002) 4421: 256-262 4) Ichihara YGInternet Imaging (2001) 4311: 419-426 1 E-mailmaokabe@lab.nig.ac.jp 1993 1996 CREST 1997 1 E-mailitokei@nibb.ac.jp 1986 1991 ERATO 2002