Introduction ur company has just started service to cut out sugar chains from protein and supply them to users by utilizing the handling technology of

Standard PA-Sugar Chain Catalogue Masuda Chemical Industries Co., LTD. http://www.mc-ind.co.jp

Introduction ur company has just started service to cut out sugar chains from protein and supply them to users by utilizing the handling technology of anhydrous hydrazine. This protein deglycosylation purification technology has reached completion through the joint research with the Department of Functional Glycomics, Life Science Research Center, Kagawa University. At present, approximately 50 kinds of the purified products of core sugar chains after pyridylamination (PA) have been obtained. In addition, a mass production process through the scale-up of hhydrazinolysis is under development. 2

Supply Service of Standard Sugar Chains The hydrazinolysis method, which was developed as a C-terminal amino acid determination method of protein, has widely been utilized after various improvements as a quantitative method to cut out sugar chains. HN CH NH NH 2 NH 2 H NHNH 2 Ac 2 H, H NH 2 NH 2 NaHC 2-Aminopyridine H N Me 2NH-BH N H NH N ur company can supply abundant standard sugar chains using the protein deglycosylation technology developed by the joint research with the Department of Functional Glycomics, Life Science Research Center, Kagawa University.

Supply Service of Sugar Chains with Reactive Groups PA ur company can also supply amino compounds or hemiacetal compounds with reactive groups through the reduction of the PA terminals of standard sugar chains. H NH N H, H NHA c H NH2 Supply Service of Hhydrazinolyed Products ur company conducts a consultation for the work to apply hydrazinolysis to specific proteins or entrusted proteins in our facilities and supply the generated crude fractions, crude products, or purified products. Purified products Specific Protein Crude fraction (mixture) Hydrazinolysis Separation and purification 4

Catalogue for Standard Sugar Chains in vitro Prices and delivery date are based on a consultation. Mass supply is also upon consultation. These standard sugar chains can be used for in vitro research only. A separate contract is necessary for the purpose of commercial use. 5

PA- 001-2 -2 Glc 1--2-2 PA- 002 Gal 1-4GlcNAc 1-2 GlcNAc 1-4 Gal 1-4GlcNAc 1-2 PA- 00 Gal 1-4GlcNAc 1-2 GlcNAc 1-4 Gal 1-4GlcNAc 1-2 PA- 004 Gal 1-4GlcNAc 1-2 Gal 1-4GlcNAc 1-2 PA- 005 GlcNAc 1-2

PA- 00 GlcNAc 1-2 GlcNAc 1-4 GlcNAc 1-2 PA- 008 Gal 1-4GlcNAc 1-2 GlcNAc 1-2 PA- 009 Gal 1-4GlcNAc 1-2 PA- 010 GlcNAc 1-2 GlcNAc 1-2 PA- 011 GlcNAc 1-2 Gal 1-4GlcNAc 1-2 7

PA- 012 Gal 1-4GlcNAc 1-2 Gal 1-4GlcNAc 1-2 PA- 01 Gal 1-4GlcNAc 1-2 Gal 1-4GlcNAc 1 Gal 1-4GlcNAc 1 4 2 PA- 014 Gal 1-4GlcNAc 1-2 Gal 1-GlcNAc 1 Gal 1-4GlcNAc 1 4 2 PA- 015 Gal 1-4GlcNAc 1 Gal 1-4GlcNAc 1 Gal 1-4GlcNAc 1 Gal 1-4GlcNAc 1 2 4 2 PA- 020 GlcNAc 1-2 GlcNAc 1-2 8

PA-024 Neu5Ac 2-Gal 1-4GlcNAc 1-2 Gal 1-4GlcNAc 1-2 PA- 025 Gal 1-4GlcNAc 1-2 Neu5Ac 2-Gal 1-4GlcNAc 1-2 PA- 02 Neu5Ac 2-Gal 1-4GlcNAc 1-2 Neu5Ac 2-Gal 1-4GlcNAc 1-2 PA- 029 PA- 00 9

PA- 01-2 PA- 02-2 -2-2 PA- 0-2 -2-2-2 PA-05-2 -2 PA-0-2-2 10

PA- 041 PA- 042 PA- 04 Gal 1-4GlcNAc 1-2 PA- 044 GlcNAc 1-2 GlcNAc 1-4 GlcNAc 1-2 PA- 045 Gal 1-4GlcNAc 1-2 11

PA- 04 Gal 1-4GlcNAc 1-2 GlcNAc 1-2 PA- 047 GlcNAc 1-2 Gal 1-4GlcNAc 1-2 PA- 048 Gal 1-4GlcNAc 1-2 PA- 049 PA- 050 GlcNAc 1-2 12

PA- 051 GlcNAc 1-2 PA- 055 GlcNAc 1-4 GlcNAc 1-2 PA- 057 Neu5Ac 2-Gal 1-4GlcNAc 1-2 Neu5Ac 2-Gal 1-4GlcNAc 1-2 PA- 058 Neu5Ac 2-Gal 1-4GlcNAc 1-2 Neu5Ac 2-Gal 1-4GlcNAc 1-2 PA- 059 Neu5Ac 2-Gal 1-4GlcNAc 1-2 Neu5Ac 2-Gal 1-4GlcNAc 1-2 1

PA- 00 Neu5Ac 2-Gal 1-4GlcNAc 1-2 Gal 1-4GlcNAc 1-2 PA- 01 Gal 1-4GlcNAc 1-2 Neu5Ac 2-Gal 1-4GlcNAc 1-2 PA- 0 Gal 1-4GlcNAc 1-2 PA- 07 Neu5Ac 2-Gal 1-4GlcNAc 1-2 PA- 08 GlcNAc 1-4 GlcNAc 1-2 14

PA-05 GlcNAc 1-2 GlcNAc 1-4 GlcNAc 1 4 2 GlcNAc 1 PA-02 Gal 1-4GlcNAc 1-2 Neu5Ac 2-Gal 1-4GlcNAc 1-2 PA-0 Neu5Ac 2-Gal 1-4GlcNAc 1-2 Gal 1-4GlcNAc 1-2 PA-08 Neu5Ac 2-Gal 1-4GlcNAc 1-2 Neu5Ac 2-Gal 1-4GlcNAc 1-2 PA-075 GlcNAc 1-2 15

PA-07 Gal 1-4GlcNAc 1-2 PA-077 GlcNAc 1-2 PA-078 PA-505 Neu5Ac 2-Gal 1-4GlcNAc 1-2 PA-507 Neu5Ac 2-Gal 1-4GlcNAc 1-2 1

PA-508 GlcNAc 1-2 Neu5Ac 2-Gal 1-4GlcNAc 1-2 PA- 514 Neu5Ac 2-Gal 1-4GlcNAc 1-2 GlcNAc 1-2 PA- 541-2 -2 Glc 1-2Glc 1-Glc 1--2-2 PA- 084 Gal 1-4GlcNAc 1-2 PA- 085 Gal 1-4GlcNAc 1-2 17

Column : NACALAI Tesque, CSMSIL Packed Column for HPLC, 5C18-AR-00 ( * 150 mm) Solvent A : 20mM of AcHaq.-NHaq. (ph 4.0) Solvent B : Solvent A containing 1.0% of n-buh. Gradient : % > 40% B (0 > 45min.) Flow Rate : 2.0 ml/min. Detection : Fluorescence (Ex: 20nm, Em:400nm) Column Temp.: 25C Injection : 10 pmol PA-0 PA-020 PA-00 PA-00 18

70-005 4 12 52 TEL. 087-851-107, FAX. 087-851-9749 URL http://www.mc-imd.co.jp Masuda Chemical Industries Co., Ltd. 4-12-52 Asahimachi, Takamatsu City, Kagawa Prefecture 70-005 tel : 087-851-107 fax. 087-851-9749 URL http://www.mc-ind.co.jp