11 8 25 passive passive active 1 ( ) LTP 1 1) 2) 1 1
1997 CA1 12 13 1990 2 K A K 1997 transient 3 transient 4-aminopyridine 4- AP A K A A [ 1 conductivity recording transient 4 ] 2 ( K ) i) A K CA1 A K transient D K delay current A K 5 6 whole cell 2-4 CA1 whole cell 7 300 m 100ms 150mV A K A current A K 2
whole cell A current Na current 10ms 8 A K blocker 4-AP 5 mmol Na current kinetics 4-AP CA1 A K A current ii) A K A current 100ms 9 10ms A current 50ms 200ms A current EPSP modulation iii) EPSC A K blocker 7, Input1 EPSC excitatory post-synaptic current 10 EPSC AMPA A current 20ms EPSC 50ms 200ms 50ms 20ms EPSC A current EPSC amplitude EPSC Time to peak T(1/2) 11 EPSC A current EPSC A K blocker 4-AP EPSC 12 7, Input2 EPSC 13 A current 3
A current Acurrent EPSC 14 Acurrent epsc input1 1 1 4-AP epsc input2 A current whole cell A current A current A current K Q. 100ms natural A current A. LTP Protein kinase C protein kinase A A K modulation tetanus protein kinase A current modulation Q. A. 4-AP 4-AP pre-synaptic K modulation A current 3 A D K A K 4-AP D current Storm et al 1990 A D Hines M. neuron 4
1: 2: Storm et al, 1990 3: Homan et al, 1997 4: Table 7.1 Voltage gated ionic currents in cortical neurons 5: 6: 7: Materials and Methods 8: control, 4-AP, wash 9: interval 10: EPSC 11: EPSC 12: 4-AP EPSC 13: Input2 14: A current epsc input2 5
EPSP A EPSP 600 m EPSP 15 Hodikin-Huxley Hodikin-Huxley 600 m EPSP 16 4-AP A current Dcurrent [ A Spine primary culture A EPSP ] A K D K Hodikin- Huxley 17 Storm whole cell peak conductivity A current 18 D current EPSP EPSP A K D K A K EPSP 19 A D K EPSP D K D K 20 D K A K A K D K A current A K 6
4 2 EPSP A D K EPSP 2 A current D current 21 20 ms EPSP 2 17 g KA =0:03 S=cm 2 g KD =0:05 S=cm 2 A D g KA =0:03 S=cm 2 g KD =0:05 S=cm 2 2 1:1 1 2 ( ) Passive 2 EPSP A K D K 1 EPSP ( 21 ) 1 EPSP A D K 2 EPSP ( ) D A (KA and KD) 1 D 2 A A D 22 A D A D EPSP 1 `Single' EPSP 1 D K (20 ms, 50 ms) 2 2 A K D 100 ms, 200 ms EPSP D 2 D K A K 2 Q. A current D current A. I-V ( 18 ) D current D current 070 mv A current 050 mv 7
070 mv 1 EPSP A current Q. 1 A. 18 EPSP D A current 18 A current EPSP 18 Q. D current A current 2 A current A. 1 EPSP D current Acurrent D current A current EPSP ( 23) g max 1 D current 2 A current Q. 2 EPSP A current EPSP A current EPSP g max 20 A. 8
Q. l h A. Q. 2 EPSP D current D K A current D current A. 18 A current EPSP Q. 1 A K 2 D K A. Q. EPSP A. EPSP 2 passive 070 mv 18 D K 1 EPSP Q. EPSP A K A. EPSP A Q. 21 A current 2 EPSP A current A. EPSP 600 m 9
2 A K 5 EPSP EPSP 20 ( 24) EPSP 20 600 m EPSP EPSP ( 24 ) A D K EPSP g max EPSP ( 24 ) A K 3 D K 20 2 EPSP EPSP 2 Q. 3 A. 3 3 1 2 1 D 2 A K 200 ms D K EPSP 24 2 2 10
15: 16: EPSP 17: Parameters for the conductance of KA and KD channels 18: A-type K channel D-type K channel 19: 20: KA, KD, KA+KD g max 21: 2 EPSP `Passive' `KA' `KD' `KA and KD' 4 22: `KA channels' EPSP interval KA=(KA+ KD) `KD channels' EPSP interval KD=(KA + KD) 23: 20 ms 50 ms 200 ms KA KD KA and KD g max Decrease 4 (= 10 ms 20 ms 40 ms 60ms) 9 11
Q. K A. EPSP 2 20 2 ( 25) Q. A. Q. 2 A. 24 0:03 S=cm 2 0:05 S=cm 2 A current D current D current A current Q. A. 10 ms g max EPEP 5{15 ms g max 0.01{0.04 ( 25) g max 12
6 LTP ES potentiation active dendrite LTP model LTP LTP EPSP 1 ES potentiation (EP potentiation) E EPSP S P population spike LTP EPSP population spike population spike preliminary LTP ( 26) CA3 CA1 EPSP 1 27 MED probe slice 28 1, 2, 3 EPSP 29 A E-1, E-2, E-3 150 m EPSP 29 B E-1 E-3 EPSP 100 Hz 1s LTP 3 EPSP 30 5 E-1 LTP ( ) E-1 EPSP 2.0 E-2 2.2 2.3 E-3 2.5 EPSP 3 ( 31) 3 EPSP LTP 13
Q. A. EPSP 32 NMDA APV AMPA CNQX TTX EPSP Q. 1 A. Q. A current EPSP A. Q. A current D current A. A current 300 m 4 D Q. 4 A. 3 1 Q. Active dendrite A. back propagation 14
back propagation 15
24: KA KD KA+KD 2 g max ( 5ms 0.02 S 15 ms 0.03 S) 20 EPSP 6 25: ` ' KA KD KA+KD g max 3 3 26: `Fig. 1 Brain Slices Hippocampus' 3 27: `Fig. 3 MED system components' 28: `Fig. 4 Hippocampus on MED' (. ) 29: `Fig. 9Simultaneous Recordings from Three Electrodes' 30: `Fig. 10 Dynamical Changes on Evoked Responses' 31: `Fig. 11 Curve Fitting to a Single Exponential Curve' 32: (`Fig. 7 Pharmacological Experiments' A) 16