LSM5Pascal Ver 3.2 GFP 4D Image VisArt 2004.03
LSM5PASCAL V3.2 LSM5PASCAL SW3.2Axiovert200M 1 1 2 3 3 4 4 5 SingleTrack 9 Multi Track 10,18 5 / 21 6 3 27 7 35 8 ( OFF) 40
LSM5PASCAL V3.2 LSM5PASCAL 65 30 5 ECU MO
( ) On PC Ar HeNe ON PC Windows Log in HeNe 543nm G 90 HeNe 633nm R 405nm:V Page 1
Ar 458488514nm B Ar Power Enable ON 90 Standby on Power 1011 11 PC 5 Page 2
Pascal OnlineMode Start Online Mode Start Offline ModePC Start Offline Mode Online Mode Page 3
7 File Save/Load Exit Acquire Process 3D ViewAcquire D Macro MacroEditor Options Maintain Page 4
LSM5Pascal Acquire Page 5
Laser Control Page 6
Microscope Setup Acquire Micro PC VIS 7 Page 7
Transmitted Light ON/OFF Reflected Light ON/OFF Objective Reflector / Reflector Turret Page 8
Configuration LSM 2 Single Track Multi Track Acquire Config Single Track Single Track Store/Apply Apply Configuration B FITC G Rhodamin R Cy5 Page 9
Multi Track Single Track Multi Track Scan Control Acquire Scan Multi Track 1 Page 10
Scan Control 2 Scan Control Acquire Scan Mode Channel AOTF Page 11
Find / LSM Acquire Scan Channels Pinhole Pinhole Optical Slice Find Find Cont. Find Page 12
Find Saturation Scan Control Channels Cont. Stop Detector Gain Ample.Offset Ample.Offset ( ) Stop Page 13
Scan Mode Mean Number Line Frame Single Total ModeLine Frame Method Mean Sum Number Page 14
Scan Control New : Find : Fast XY : Scan Speed10 Single : 1 1 Stop : Cont. Cont. Continue : Stop Page 15
Mode Objective Lens Image Size Image Size Speed 13 12 Pixel Depth, Scan Direction Scan Average 8 or 12 bit Frame Line Zoom, Rotation Offset Channnels Page 16
Channel Data Pinhole[m] Optical Slice Z Airy Unit Optical Slice Airy 1 Detector GainAmplOffsetAmplGain & Excitation MOTF MOTF 488nm 510 Page 17
Multi Track ( ) LSM5PASCAL Single Track Multi Track 2 Single Track Rhodamine FITC Multi Track 1 Single Track Find Page 18
Aquire Config Multi Track Multi Track Config Single Track Apply Find Single Track Find1 Multi Track Pinhole Find Find Page 19
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LSM5Pascal V3.2 Page 21 5 LSM5PASCAL 2 Windows Zeiss - Image Browser http://www.zeiss.de/imagebrowser Export 2 A A: 3.5 C C: Windows LSM D D: I I: CD-RW CD NeroBurningROM : MO 640MB Windows C/D LSM510 D MO HDD MO MO
5 File New -.mdb fig.1.mdb.lsm fig.2 Open fig.1 Page 22
File Save OK Save 5 Open - /.mdb.lsm mdb LSM510 ImageBrowser Page 23
File Open Open Reuse Apply Save Page 24
6 TIFF/JPEG etc Export File Export TIFF Tagged Image File Save Image Type Image Type Raw data single series Series 000.tif 001.tif 002.tif 3 Contents of the Image Window single series 10241024pixel Full resolution image window single series Page 25
Import File Import Single Series All Supported File Open Page 26
6 3D 6 Z Sectioning Scan 6 Sectioning Scan Control Z stack Z setting Page 27
Mark First/Last XY Cont. Mark First Mark Last Scan Stack Z Size Page 28
(2) Z Slice Optimal Interval Optical Interval Num Slice Start Start Average Page 29
6 SliceAnim OrthoCut Depth Coding 1 Rotation Stereo Slice Anim Gallery Slice Anim Slice Anim Slice Gallery Page 30
Ortho Cut 2 Ortho Cut 5 Page 31
Depth coding 3D view DepthCod ThresholdContrast Brghtness Apply Scale Bar Page 32
3D Projection Sectioning 1 3D View Projection First Angle0 First Angle Number Projections 32 Difference Angle 6 90 180 Apply Anim 1 First Angle Number Projection Difference Angle Page 33
Stereo Image 3D 3D Stereo Mode 2 Red/Green Image Split Image Right Left Angle 510 Apply Number Image Difference Angle Page 34
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1 9 HAL 2 10 FL 3 11 4 1214 5 15 6 16 7 8 Page 36
/ HAL on/off ON H H Page 37
2 DIC DIC 3DIC DIC DIC LSMDIC LSM Page 38
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ON HAL on/off OFF FL on/off Page 40
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