GeneQuant pro GE imagination at work 71-2236-32
....3...4...4... 5...5...6...6...7...8 DNARNA... 8... 8... 9... 10... 11 595546562 nm... 11 UV280 nm... 12.... 13... 14... 14.... 15 DNARNAoligo... 16 Base Sequence... 18 Tm... 19 Protein 595 Assay... 20 Protein 280 Measurement... 22... 23... 24... 25... 25 PC Windows... 25
... 27... 27... 28... 28... 29... 29... 29... 30... 31... 31 cal check... 31... 32... 32... 32... 32... 33... 34... 35
10 40 80% 3180 50% 31 40 4 kg 5 cm / 90 265 V 1 Instrument Ready 3
1 2 3 1. 2. 1 on0 off 3. 4
GeneQuant pro 1 230260280320 546562595 600 nm PCR DNARNA μg/mlng/μlμg/μlpmol/μlpmol phosphate 260 nm 230 280 nm 260/230 260/280 320 nm PCR nearest neighbour Breslauer et al., Proc. Natl. Acad. Sci. U.S.A. 83, 37461986 595 nm546 nm 562 nm BCA 600 nm PC Ecxel GLP PC 5
128 64pixel set-up 28 3 Instrument Ready enter abs 12 set-up 14 set-up cal check 31 select print Base Sequence DNA RNA oligo PC base seq 18 Tm stop set ref enter 260 nm Tm 19 escape set-up 6
abs 230260280320595600 nm DNA 230260280320 nm DNA 260/280 260/230 RNA 230260280320 nm RNA 260/280 260/230 oligo 230260280320 nm / 260/280 260/230 protein 595 assay Set-up 595546562 nm set-up protein 280 meas 260280320 nm cell culture 600 nm OD 7
DNARNA DNA RNA 10 mm 260 nm 1.0 5040 μg/ml 33 μg/ml Base Sequence = A 260 DNARNA 5040 33 10 mm set-up 27 μg/ml set-up ng/μlμg/μlpmol/μl pmol phosphate pmol/μl PCR 1 μg/ml = 1 ng/μl = 0.001 μg/μl pmol/μl = μg/ml 1,000 pmol phosphate = μg/ml 315 DNA MW MWg/mol= da 312.2dC 288.2dG 328.2dT 303.2 MW RNA dt 303.2dU 298.2 5' 3' 17 2 MW 61 MW g/molna23.0k 39.1TEA102.2 8
260/280 DNA RNA 1.8 2.0 260 nm 280 nm 280 nm 260 nm 260/280 260 nm 280 nm 0.05 1 230 nm 230 nm Tris EDTA RNA 260/230 2.0 RNA 230 260 nm RNA 260/280260/230 10 mm set-up 9
260280 nm 320 nm set-up A 260 A 280 A 320 = A 260 A 320 = A 260 A 320 /A 280 A 320 = A 260 A 320 /A 230 A 320 27 SWIFT 17 260 nm 230 nm 260 nm280 nm 320 nm 10
595546562 nm 595 nm BSA 546 nm BCA bicinchoninic acidbca 562 nmbca 546 595 nm 2.000 0 1 0.0 0 2 2 3 2 6 0.95 1.00 11
UV280 nm 280 nm A 280 280 nm Christian Warberg Biochemisceh Zeitung 310, 3841941 260 nm mg/ml = 1.55 A 280 0.76 A 260 = 1 A 280 2 A 260 280 nm 320 nm set-up 2 260280 nm 2 260 nm 0 280 nm 2 0 1 BSA 1 = 1.115 0 0.8 mg/ml mg/ml= 1.115 A 280 A 280 HiTrap 12
600 nm 0.4 0.600 OD OD OD OD 1OD 600 = 8 10 8 cells / ml GeneQuant pro OD OD OD 2.0 set-up 10 mm 13
set-up set-up abs select enter stop No Sample No. 11 PrinterSerialPC None PCAuto Print print PrintSurvey Scan DNARNAoligo EnglishDeutschFrancaisEspanol Italiano 14
base seqtmabsdnarnaoligoprotein 595 assayprotein 280 meascell culture set ref 0.000... enter... set up print PC print 15
DNARNAoligo 8 Pathlength 105210.5 mm Units) 320 nm Use 320nm Dilution factor Insert sample μg/mlng/μlμg/μlpmol/μlpmol pmol/μlpmololigo 320 nmuse 320 nm 1 10,000 set ref enter DNA/RNA 230260280320 nm 260/230260/280 ------ stop oligo Default Factor Calculated Factor Use Default Factor Insert sample 33 μg/mlssdna 37 μg/ml set ref enter oligo 260/230260/280 stop 16
220 330 nm 10 230260280 320 nm select set-up select 0.1 0.20.51.02.02.5Auto enter DNA Determination Date : 28 May 2003 Name : Factor : 50.0 Units : μg/ml Pathlength : 10 mm Dilution Factor : 1 Use 320 nm : No No. 230 nm 260 nm 280 nm 320 nm 260/280 260/230 Conc. 1 0.230 0.471 0.255 ----- 1.850 2.045 23.5 Oligonucleotide Determination Date : 28 May 2003 Name : Dflt Factor : 33.0 Units : μg/ml Pathlength : 10 mm Dilution Factor : 1 Use 320 nm : No No. 230 nm 260 nm 280 nm 320 nm 260/280 260/230 Conc. 1 0.168 0.325 0.183 ----- 1.780 1.934 10.7 17
Base Sequence 8 Base Type) Phosphorylated Counter Ion) MW Other MW Theor. Abs MW Calculated MW) Calculated Factor DNARNA NaKTEA Other Other 1.0 1,000.0 enter ACG T/U 10 64delete cancelselect select AU/μmol pmol/μl μg/ml Base Sequence Date : 28 May 2003 Name : AGC, AGC, AGC, AGC, AGC Base Type : DNA Phosphorylated : Yes Counter Ion : Na Theor. Abs : 149.1 Calculated MW : 5051.0 Calculated Factor : 33.9 18
Tm 8 10 mer Primer Conc. Buffer Molarity Tm Calculated Tm Insert sample 1.0 100.0 pmol/μl 0.1 10.0 nearest neighbourtm set ref enter Tm enter Tm Tm nearest neighbour stop Tm Date : 28 May 2003 Name : AGC, AGC, AGC, AGC, AGC Phosphorylated : Yes Counter Ion : Na Primer Conc. : 1.0 Buffer Molaryty : 0.1 Calculated Tm : 57.0 deg.c No. 260 nm Meas. Tm 1 0.123 57.8 19
Protein 595 Assay 8 Method No. of Standards Wavelength Pathlength Units New Standards? Std#1 Insert Std#1 Std#2 Insert Std#2 Insert Sample Bradford 595Biuret 546BCA 562 3 27 105210.5 mm μg/mlmg/mlμgmg/ml set ref 0.0 100.0 1enter enter 0.0 100.0 2enter enter Y set ref enter stop 20
Protein 595 Date : 28 May 2003 Name : Method : Bradford No. of Standards : 6 No. Abs. Conc. 1 0.693 0.139 2 0.842 0.278 3 0.934 0.417 4 1.026 0.556 5 1.150 0.650 6 1.285 0.834 Slope : 0.746 Corre. Coeff : 0.996 Intercept : 0.593 No Abs. Conc. 1 0.995 0.520 2 0.162 21
Protein 280 Measurement 8 1Coeff 1 2Coeff 2 320 nm Use 320 nm Insert sample 280 nm0.01 10.00 1.55 260 nm0.01 10.00 0.76 320 nm set ref enter protein 280 means stop Protein 280 nm Measurment Date : 28 May 2003 Name Coeff. 1 : 1.115 Coeff. 2 : 0.000 Use 320 nm : No : No. 260 nm 280 nm 320 nm Conc. 1 0.123 0.275 ----- 0.307 22
8 Factor Insert sample 1.0 1000.0 2.0 set ref enter cell culture 600 nmod stop Cell Culture 600 nm Date : 28 May 2003 Name : Factor : 2.0 No. OD600 Result 1 0.857 1.714 23
>2.0A Absorbance >2.0A Invalid Curve Solution too dilute Too much light 1 Ref.1 error 2 Ref.2 error Lamp failure Protein 595Cell Culture2.000 A (100 μg/ml0.1 mg/ml) Lamp too hot Filter error Grating error 24
Seiko DPU-414 80 Seiko DPU-414 DIP SW On print Seiko DPU-414 set-up Print PC Windows 1 80-2109-02 Excel 2Hyperterminal 80-2109-02 Serial Hyperterm.exe GeneQunat pro OK Com 1 OK COM1 / 19,2008 1OK ASCII ASCII OK 2 25
Excel 26
10 mm 0.005 3.000 0.100 1.000 0.05 GeneQuant pro 15 mm 8.5 mm ng/μl 1 μl mm 5 125 > 2,000 10 80-2002-58 > 750 10 80-2002-77 > 70 10 80-2103-69 > 70 70 μl UV 10 80-3000-81 10 250 > 5 2 5 80-2103-68 100 2,500 3 > 3 100 0.5 80-2104-66 80-2104-67 1 dsdna 10 mmabs260 = 1.050 μg/ml = ng/μl 2 80-2109-87 3 DNA PCR 50 200 ng/μl 7 μl dsdna 2 μg/ml 0.020 7 μl 14 ng dsdna dsdna 20 μg/ml 0.0203 μl 60 ng dsdna 70 μl UV RNA DNA 70 μl 27
GeneQuant pro 5 nm 10 mm 20 mm 80-2103-69 70 μl 80-2103-68 7 10 μl 3 80-2104-6680-2104-67 70 μl UV 80-3001-81 70 μl 28
10 mm80-2103-68 5 mm 0.5 mm 0.1 M NaOH 0.1 M HCl Decon 4 10 1 320 nm A 260 0.100 29
80-2109-86 80-2109-96 72-0496-01 80-2071-87 71-1600-04 80-2109-02 80-2109-88 80-2109-13 30
GLP/GMP ISO 9001 cal check Good Laboratory PracticeGLP GLP 80-2109-88 230260 280320595 600 nm 260280 nm 260 nm NIST 2 80-2109-88 31
/ 1.6AT5 mm 20 mmfst2 32
36 80-2109-86 1 2 7 3 4 A B 2 5 C B 2 A 6 7 7 8 33
Fixed at 230, 260, 280, 320, 546562, 595 and 600 nm Survey scan : 220 330 nm Czerny - Turner configuration with 1,200 lines/mm holographic grating Automatic upon switch on 5 nm 1 nm 0.5 nm Long life borosilicate glass pulsed duterium lamp Silicon photodiode 0 to 3.000 A for 230, 260, 280 and 320 nm 0 to 2.000 A for 546562, 595 and 600 nm 1.0% or 0.005 A to 3.000 A, whichever is the greater 0.5% of absorbance value < 0.1% T at 220 nm using Acetone Centronics parallel as standard 9 pin serial via interface adapter lead 320400160 mm 4 kg 90 265 V AC, 50/60 Hz, 80 VA Safety standard EN 61010-1 EMC emissions EN 50081-1 Generic emissions part 1 EMC immunity EN 50082-1 Generic immunity part 1 Susceptibility standard IEC 801 Quality System Designed and manufactured in accordance with an ISO 9001 approved quality system 34
12 Biochrom Ltd., 22 Cambridge Science Park, Milton Road, Cambridge CB4 0FJ, UK 35
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