* * Fischer 344 Elastica-Masson trichrome NIH Image K + phenylephrine carbachol QOL quality of life
Fischer 344 camp, Elastica-Masson trichrome Photoshop Adobe
Louis, MO, USA) atropine sulfate mono- O2CO2 hydrate, potassium chloride Krebs-Henseleit ml organ bath 7T-15-240 mmnacl, KCl, NaHCO, MgSO 4, KH 2 PO 4, glucose, organ bath g Welch's -test KCl mm KCl K + K + Krebs-Henseleit NaCl, KCl mm Elastica-Masson trichrome carbachol propranolol 10-6 M n phenylephrine 10-8 M 10-4 M phenylephrine prazosin 10-7 M n phenylephrine K + prazosin 10-7 M propranolol 10-6 MK + carbachol 10-10 M 3 10-3 M carbamylcholine chloride, phenylephrine ver.5.0 NIH Image ver.1.63 hydrochloride, prazosin hydrochloride, propranolol hydrochloride Sigma chemical Co. (St. ascorbic acid, CaCl 2, ph Student's -test P atropine 10-7 Mn n n n n g /mm 2 n g /mm 2 n g / mm 2 n g / mm 2 n K +
會田 今野 石幡 片野 図２ ラット膀胱体部および三角部における 筋組織 線維組織の割合 縦軸 組織面積の割合 若齢ラット 老齢ラット N.S. not significant 内は実験例数 値は 平均値±標準誤差で表している 図３ ラット膀胱体部および三角部における 高濃度 K+ による最大収縮反応 縦軸 収縮張力 若齢ラット 老齢ラット N.S. not significant 内は実験例数 値は 平均値±標準誤差で表している 52
phenylephrine K + phenylephrine phenylephrine prazosin prazosin10-7 M prazosin prazosin10-7 M *P. vs phenylephrine carbachol K + phenylephrine n n P 6),7) EC 50 10-6 M 10-6 M phenylephrine 1 prazosin carbachol n n EC50 10-7 M, 10-7 M 4)
carbachol K + carbachol carbachol 8),9),10) 12) Elastica- KCl Masson trichrome
Ca 2+ Ca 2+ KCl KCl mm K + Ca 2+ 1 1 A K + phenylephrine carbachol carbachol EC50 phenylephrine Ordway 13) Fischer 344 Ordway 8) Fischer 344 phenylephrine, norepinephrine, clonidine acetylcholine bethanechol oxotremorine Suzuki 10) EC50 Fischer 344 phenylephrine Fischer 344 1 2 17) 1 14) phenylephrine 1 prazosin 1 KCl 1 1 A 1 3 10-5 3 10-4 M 15),16) Suzuki 10) 1 A EC50 2),3)
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Yamagata Med J 2005 23 (1) 49-57 Tomomi Aita, Kinuko Konno, Akira Ishihata*, Yumi Katano Course of Clinical Nursing, *Course of Organ Functions and Controls, Yamagata University School of Medicine, Yamagata, Japan ABSTRACT The urinary bladder dysfunction increases with aging. We previously reported that -adrenoceptors-mediated contraction increases in aged trigone. To further elucidate the mechanisms of age-related urinary bladder dysfunction, we studied the contractile response to phenylephrine and carbachol, in addition to histological examination. Urinary bladders from young (2-3 months) or aged (27 months) male Fischer 344 rats were isolated and the developed tension was recorded. In Elastica- Masson trichrome staining, smooth muscle and collagen were assessed quantitatively using NIH Image. The percentage of smooth muscle and collagen area was not different between young and aged rats. High K + -induced contractions were not significantly changed by aging. Phenylephrine elicited greater contractions in the aged body than those in young rats. In contrast, carbachol-induced contraction in trigone were not significantly changed by aging. It has been reported that relaxing response mediated by -adrenoceptors was decreased in the aged body. These results suggest that the facilitation of contractile response mediated by -adrenoceptors in both body and trigone and the decrease in relaxation to -adrenoceptors in body may be a cause of dysfunction in the aged urinary bladder. Key words : bladder, smooth muscle, aging, -adrenoceptors