Welcome to a ore Colorful World BD BD Horizon Brilliant Violet x 1, Lymph x 1, CD3+ CD8 PerCP-Cy5-5-A CD4+ 5 1

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1 Brilliant Violet マルチカラー トレーニング キャンペーン BD Brilliant Violet 2 ( ) 1 ( ) P12 Brilliant Violet

2 Welcome to a ore Colorful World BD BD Horizon Brilliant Violet x 1, Lymph x 1, CD3+ CD8 PerCP-Cy5-5-A CD FSC-A 2 25 x 1, CD3 APC-H7-A CD4 Brilliant Violet 51-A CD25 Brilliant Violet 421-A CD25+++ CD25++ CD25 Brilliant Violet 421-A CD25+++Foxp3+ CD45RA FITC-A CD4 Brilliant Violet 51-A Foxp3 PE-A CD25 Brilliant Violet 421-A CD127 PE-CF594-A CD127 dim CD25 high CD25 Brilliant Violet 421-A -149 CD25 Brilliant Violet 421-A CD45RA- CD45RA CD45RA FITC-A 1 5 Count CCR7 PE-Cy7-A 1 5 BD Horizon Brilliant Violet Treg 9 PBC anti-human CD3-APC-H756275anti-human CD4-Brilliant Violet anti-human CD8-PerCP-Cy anti-human CD25-Brilliant Violet anti-human CD127-PE-CF anti-human CD45RA-FITC anti-human CD197 CCR7 -PE-Cy BD Transcription Buffer Set test / tests anti-human Foxp3-PE5682 CD3 CD4 CD4 T 2

3 BD TEL x 1, 25 2 Splenocyte x 1, 25 2 x 1, FSC-W 15 1 SSC-W 15 1 Singlet Singlet FSC-A x 1, FSC-H x 1, SSC-H x 1, x 1, CD3e- CD3e+ CD8a PE-A CD8a+ B22 PE-Cy7-A CD45R/B22+ NK , CD3e APC-Cy7-A 1 5-1, CD CD4 Alexa Fluor 488-A -1,45 B22-NK ,561 NK11 Brilliant Violet 421-A Ly-6G PE-CF594-A Ly-6G+Ly-6C LY-6G+ Ly-6G-Ly6-C Ly-6G+ CD11b APC-A CD11b+ -5, Ly-6C Ly-6G Brilliant Violet 51-A Ly-6C Ly-6G Brilliant Violet 51-A 2 BD Horizon Brilliant Violet TBNK 8 BALB/c ouse BD FcBlock553142anti-mouse CD3e-APC-Cy anti-mouse CD4-Alexafluor anti-mouse CD8a-PE anti-mouse NK1.1-Brilliant Violet anti-mouse CD45R/B22-PE-Cy anti-mouse Ly-6C/Ly-6G-Brilliant Violet anti-mouse Ly-6G-PE-CF anti-mouse CD11b-APC Cell Viability Solution

4 BD Horizon Brilliant Violet Dyes BD Horizon Brilliant Violet BD BV421 BV51 BV65 BV65 BV711 5 BV786 3PE T / PFA EDTA nm BD D Horizon V45 BD Horizon o i o BV BD Horizon o V5 BD Horizon BD Horizon BV51 BV65 BD Horizon BV65 BD Horizon BV711 BD Horizon BV Wavelength (nm) 4

5 Count Count Count Count Count CD25 Brilliant Violet 421-A CD25 FITC-A CD25 PE-A CD25 APC-A CD25 V45-A x 1, CD25++ CD x 1, x 1, x 1, x 1, CD25 Brilliant Violet 421-A CD25 FITC-A CD25 PE-A CD25 APC-A CD25 V45-A CD25 anti-human CD4-PerCP-Cy anti-human CD25 Brilliant Violet FITC 5699PE557138APC56987BD Horizon V CD4 BV421 BV65 BV BV51 BV65 BV65 APC AF7 BV711 AF7 PerCP-Cy 5.5 BD BD Brilliant Violet Web Page tech_cell@bd.com 1 * BV , / 5 BV / 5 BD FACSVerse BD LSRBD LSRFortessa BD FACSAria BD Influx BD FACSCanto BD FACSVerse BD LSRBD LSRFortessa BD FACSAria BD Influx BD FACSCanto BD FACSJazz BV / 2 BD LSRBD LSRFortessa BD FACSAria BD Influx BV / 2 BD LSRBD LSRFortessa BD FACSAria BD Influx BV / 5 BD LSRBD LSRFortessa BD FACSAria BD Influx BV / 6 BD LSRBD LSRFortessa BD FACSAria BD Influx * 5

6 Akt ps473, s V45 5 tests , Alkaline Phosphatase Annexin V B4-78 V45 1 tests , V45 5 tests , V5 5 tests , B7-H4 IH43 BV421 5 tests , Bcl-2 Bcl-2/1 V45 5 tests , Bcl-6, s K V45 5 tests , BrdU 3D4 V45 5 µg , Caspase-3, Active Form CD1d s 1B1 CD2 RPA-2.1 CD3 CD3e CD3 olecular Complex CD4, s C92-65 V45 5 tests , Cyno s UCHT1 SP C11 5A2 BV421 5 µg , BV51 5 µg ,* BV421 1 tests , BV tests , BV421 1 tests , BV tests , BV51 5 tests , PB 1 µg , V45 5 tests , V45 12 tests , V45 3 tests , V5 1 tests , V5 25 tests , BV421 5 tests , BV51 5 tests , BV65 5 tests , PB 1 µg , V45 12 tests , V45 3 tests , V5 1 tests , V5 25 tests , BV421 5 µg , BV51 5 µg , BV65 5 µg , BV711 5 µg , PB 1 µg , V45 1 µg , V45 25 ug , V5 1 µg , V5 25 ug , s 17A2 V45 5 µg , s RPA-T4 SK3 RPA-T4 L2 PB Pacific Blue * GK1.5 R4-5 BV421 1 tests , BV tests , BV51 1 tests , BV51 25 tests , BV65 1 tests , BV65 25 tests , PB 1 µg , V45 5 tests , V45 12 tests , V45 3 tests , V5 1 tests , V5 25 tests , BV51 5 tests , BV65 5 tests , V45 5 tests , V5 5 tests , BV421 5 µg , BV65 5 µg , BV711 5 µg , BV786 5 µg ,* BV51 5 µg , PB 1 µg , V45 1 µg , V45 25 ug , V5 1 µg , V5 25 ug , Rat OX-35 V45 5 µg , BV421 1 tests , UCHT2 BV711 5 tests , CD5 V45 5 tests , BV421 5 µg , s BV51 5 µg , V45 5 µg , CD7 -T71 BV421 1 tests , BV421 1 tests , BV tests , BV51 5 tests ,* RPA-T8 PB 1 µg , V45 12 tests , V45 3 tests , CD8 V5 1 tests , V5 25 tests , RPA-T8 V45 5 tests , SK1 V5 5 tests , Rhe, SK1 V5 5 tests , BV51 5 µg , BV65 5 µg , BV711 5 µg , BV786 5 µg ,* CD8a s PB 1 µg , V45 1 µg , V45 25 ug , V5 1 µg , V5 25 ug , Rat OX-8 V45 5 µg , CD9 -L13 V45 5 tests , BV421 1 tests , CD1 HI1a BV51 5 tests , BV65 1 tests , CD11a s 2D7 BV421 5 µg , BV421 5 µg , BV51 5 µg , BV65 5 µg ,* BV711 5 µg , s 1/7 CD11b V45 1 µg , V45 25 ug , V5 1 µg , V5 25 ug , Rat WT.5 V45 5 µg , BV421 1 tests , BV51 5 tests , BV65 1 tests , CD11b/AC-1 ICRF44 BV65 25 tests , PB 1 µg , V45 12 tests , V45 3 tests , BV421 1 tests , B-ly6 BV711 5 tests , V45 12 tests , V45 3 tests , CD11c BV421 5 µg , BV51 5 µg , s HL3 BV65 5 µg ,* BV711 5 µg , V45 5 µg , CD13 W15 BV421 1 tests , BV51 5 tests , V45 12 tests , ΦP9 V45 3 tests , V5 1 tests , V5 1 tests , V5 25 tests , CD14 5E2 V45 5 tests , Cyno Cyno, 5E2 PB 1 µg , Bab, Dog s rmc5-3 V45 5 µg , 6

7 CD15 CD16 W6D3 HI98 3G8 BV65 1 tests , BV711 5 tests , V45 5 tests , V5 5 tests , BV421 1 tests , BV tests , BV711 5 tests , PB 1 µg , V45 12 tests , V45 3 tests , V5 25 tests , V5 1 tests , V45 5 tests , BV65 1 tests ,* BV65 25 tests ,* B73.1 V45 5 tests , CD16/CD32 s 2.4G2 BV421 5 µg , V45 5 µg , CD18 Cyno, Pig, 6.7 BV421 1 tests , Rab s C71/16 BV421 5 µg , BV421 1 tests , BV tests , HIB19 V45 12 tests , V45 3 tests , V5 1 tests , V5 25 tests , BV51 1 tests , BV51 25 tests , BV65 1 tests , BV65 25 tests , BV65 1 tests , SJ25C1 CD19 BV65 25 tests , BV711 1 tests , BV tests , BV786 1 tests ,* BV tests ,* BV421 5 µg , BV51 5 µg , BV65 5 µg ,* s 1D3 BV711 5 µg , BV786 5 µg ,* V45 1 µg , V45 25 ug , 2H7 BV421 1 tests , L27 V45 5 tests , CD2 2H7 BV711 5 tests , L27 V45 5 tests , V45 5 tests , Cyno, Bab, Pig V45 5 tests , CD21 B-ly4 BV421 1 tests , Cyno, BV711 5 tests , Bab, Pig BV421 5 µg , CD21/CD35 s 7G6 BV51 5 µg ,* BV65 5 µg ,* -L233 BV421 1 tests , CD23 BV421 5 µg , s B3B4 BV51 5 µg , BV421 1 tests , L5 BV51 5 tests , CD24 BV65 1 tests , BV421 5 µg , s 1/69 BV51 5 µg ,* BV65 5 µg ,* BV421 1 tests , -A251 BV tests , V45 12 tests , V45 3 tests , BV65 1 tests , CD25 2A3 BV65 25 tests , BV711 5 tests , BV421 5 µg , s PC61 BV51 5 µg ,* BV65 5 µg ,* V45 5 µg , BV421 1 tests , BV tests , V45 12 tests , V45 3 tests , -T271 V5 1 tests , V5 25 tests , V45 5 tests , CD27 BV51 25 tests , BV51 1 tests , BV65 1 tests , BV65 25 tests , L128 BV65 1 tests , BV65 25 tests , BV711 5 tests , BV786 1 tests ,* BV tests ,* s LG.3A1 V45 5 µg , BV421 1 tests , BV51 5 tests , BV65 1 tests , BV65 25 tests , CD28.2 CD28 BV711 5 tests , V45 5 tests , V45 5 tests , s BV421 5 µg , CD29 s, Rat Ha2/5 V45 5 µg , CD3 BerH8 BV421 1 tests , CD31 W59 BV65 5 tests , V45 5 tests , s EC 13.3 BV421 5 µg ,* BV51 5 µg , CD32 s 2.4G2 BV65 5 µg ,* BV421 5 tests , CD33 W53 BV711 5 tests , V45 5 tests , CD BV421 1 tests , s RA34 BV421 5 µg , CD36 CB38 V45 5 tests , CD37 -B371 V45 5 tests , HIT2 BV421 1 tests , BV tests , BV65 1 tests , HB7 CD38 BV65 25 tests , HIT2 V45 5 tests , s 9/CD38 also known as Ab9 BV421 5 µg , CD4 5C3 V45 5 tests , s BV421 5 µg , CD41 s WReg3 BV421 5 µg , CD41a HIP8 V45 5 tests , 1G1 BV421 1 tests , CD43 CD44 s S7 BV65 1 µg G44-26 s I7 BV421 1 tests , BV51 5 tests , V45 5 tests , BV51 5 µg , V45 1 µg , V45 25 µg , V5 1 µg , V5 25 µg , 7

8 CD45 BV51 1 tests , V45 12 tests , HI3 V45 3 tests , V5 1 tests , V5 25 tests , s 3-F11 V45 5 µg , V5 5 µg , Rat OX-1 V45 5 µg , Rhe, V45 5 tests , D V5 5 tests , CD45.1 s A2 BV65 5 µg ,* V45 5 µg , BV421 5 µg , CD45.2 s 14 V45 5 µg , V5 1 µg , V5 25 µg , BV51 5 µg ,* CD45R s RA3-6B2 V45 1 µg , V45 25 µg , BV421 5 µg , CD45R/B22 s RA3-6B2 PB 1 µg , V5 1 µg , V5 25 µg , BV421 5 tests , BV51 5 tests , HI1 BV65 5 tests , V45 12 tests , CD45RA V45 3 tests , V5 5 tests , 5H9 V45 5 tests , Rat OX-33 V45 5 µg , CD45RB s 16A BV421 5 µg , BV421 1 tests , CD45RO UCHL1 BV65 25 tests , BV65 1 tests , CD47 B6H12 V45 5 tests , CD48 TÜ145 BV421 1 tests , s H48-1 BV421 5 µg , CD49b s DX5 BV421 5 µg , V45 5 µg , CD49d Cyno, Bab, Dog, Bov 9F1 BV711 5 tests , CD49f GoH3 BV421 1 tests , BV tests , BV421 1 tests , BV tests , BV51 5 tests ,* NCA16.2 BV65 25 tests , CD56 BV65 1 tests , BV711 5 tests ,* B159 V45 12 tests , V45 3 tests , CD59 Rat TH9 V45 5 µg , CD61 s, Rat 2C9.G2 BV421 5 µg , BV65 1 tests , DREG-56 BV65 25 tests , V45 12 tests , CD62L V45 3 tests , BV421 5 µg , s EL-14 BV51 5 µg , BV65 5 µg ,* V45 5 µg , CD63 H5C6 V45 5 tests , CD BV421 5 tests , V45 5 tests , CD66 B1.1/CD66 BV421 1 tests , CD66b G1F5 BV421 1 tests ,* V45 5 tests , CD69 FN5 BV421 1 tests , BV65 1 tests , V45 5 tests , BV421 5 tests , BV421 5 µg , s H1.2F3 BV51 5 µg , V45 5 µg , CD7 s FR7 BV421 5 µg , BV421 5 µg , CD71 s C2 BV51 5 µg , BV65 5 µg , BV421 1 tests , CD73 AD2 BV tests , V45 5 tests , s TY/23 V45 5 µg , CD79a H47 BV421 5 tests , BV51 5 tests , L37.4 V45 12 tests , CD8 V45 3 tests , s 16-1A1 BV421 5 µg , V45 5 µg , HB15e BV421 5 tests , CD83 BV51 5 µg ,* s ichel-19 BV65 5 µg ,* BV711 5 µg ,* BV421 1 tests , BV tests , V45 12 tests , 2331 FUN-1 V45 3 tests , CD86 BV711 5 tests , BV51 5 µg , s GL1 BV65 5 µg ,* V45 1 µg , V45 25 µg , BV51 5 tests , BV65 1 tests , BV65 25 tests , CD9 5E1 Cyno, Bab, Pig, BV421 1 tests , Dog CD9.1 s, Rat OX-7 V45 5 µg , BV65 5 µg ,* CD9.2 s V45 5 µg , V5 5 µg , BV421 1 tests , V45 5 tests , BV421 5 tests , CD95 Cyno, Bab, Pig, Dog DX2 BV711 5 tests , V45 5 tests , s Jo2 BV421 5 µg , CD96 6F9 BV711 5 tests , Ber-ACT8 BV711 5 tests ,* CD13 BV421 5 µg , s 29 BV51 5 µg , BV51 5 tests ,* CD BV65 5 tests , V45 5 tests , CD16 s 429 VCA.A V45 5 µg , BV421 5 tests , CD17a H4A3 BV51 5 tests , V45 5 tests , s 1D4B V45 5 µg , PB Pacific Blue *

9 BV421 1 tests , YB5.B8 BV tests , 14D2 BV65 5 tests , CD117 BV421 5 µg , s 2B8 BV65 5 µg , BV711 5 µg , V45 5 µg , CD121b s 4 BV421 5 µg , BV421 5 tests , ik-β3 CD122 BV51 5 tests , s T-Beta 1 BV421 5 µg ,* BV421 5 tests , CD123 9F5 BV51 5 tests , BV711 5 tests , BV421 1 tests , BV tests , BV51 5 tests , BV65 5 tests , HIL-7R-21 BV65 5 tests , CD127 BV711 5 tests , BV786 5 tests ,* V45 5 tests , s SB/199 BV421 5 µg , SB/199q V45 5 µg , CD132 AG184 BV421 5 tests , BV711 5 tests , CD135 s A2F1.1 BV421 5 µg , BV421 5 tests , I15 BV51 5 tests , BV711 5 tests , CD138 V45 5 tests , BV421 5 µg , s BV51 5 µg , BV65 5 µg , BV711 5 µg , CD14a αr1 BV421 5 tests , s APA5 BV421 5 µg , CD141 1A4 BV711 5 tests , CD H1 V45 5 tests , s 11D4.1 BV421 5 µg , BV711 5 tests , P1H12 CD146 V45 5 tests , s E-9F1 V45 5 µg , CD147 HI6 BV421 5 tests , s RL73 BV421 5 µg , CD15 A12 BV421 5 tests , s Q38-48 BV421 5 µg , CD152 BNI3 BV421 5 tests , CD158a HP-3E4 BV711 5 tests , CD161 DX12 BV421 5 tests , CD162 s 2PH1 BV421 5 µg , CD163 GHI/61 BV421 5 tests , CD166 3A6 BV421 5 tests , BV421 5 tests , CD183 1C6/CXCR3 BV711 5 tests , s CXCR3-173 BV421 5 µg , 12G5 BV421 5 tests , CD184 BV421 5 µg , s 2B11/CXCR4 V45 5 µg , CD193 5E8 BV421 5 tests , BV51 5 tests , BV421 5 tests , CD194 1G1 BV51 5 tests , BV65 5 tests , V45 5 µg , CD195 2D7/CCR5 BV421 5 tests , V45 5 tests , CD196 11A9 BV421 5 tests , BV65 5 tests , BV421 5 tests , 1553 V45 12 tests , CD197 V45 3 tests , s 4B12 BV421 5 µg , V45 1 µg , CD2 RC OX-14 BV65 5 tests , V45 5 tests , CD29 DCN46 V45 5 tests , CD221 1H7 BV421 5 tests , CD223 s C9B7W BV711 5 µg ,* CD235a GA-R2 HIR2 BV421 5 tests , CD271 C BV421 5 tests , V45 5 tests , CD276 s IH32 BV421 5 µg , CD278 B56 BV421 5 tests , DX29 BV421 5 tests , BV421 5 tests , EH12.1 CD279 BV51 5 tests , s J43 BV421 5 µg , CD294 B16 BV421 5 tests , V45 5 µg , CD314 s CX5 BV421 5 µg , BV65 5 tests , 9E2/Nkp46 BV711 5 tests , CD335 NKp46 BV786 5 tests ,* BV421 5 µg , s 29A1.4 V45 1 µg , V45 25 ug , CD338 5D3 BV421 5 tests , BV421 5 tests , RF8B2 CXCR5 BV51 5 tests , s 2G8 BV421 5 µg , ERK1/2 pt22/py24, s, Rat 2A FLK-1 s Avas 12alpha1 FoxP3 s 259D/C7 F23 PB 5 tests , BV421 5 tests , BV421 5 µg , V45 5 µg , V45 12 tests , V45 3 tests , V45 1 tests , BV421 5 µg , V45 5 µg , G-CSF BV D2-21C11 BV421 5 tests , Granzyme B GB11 V45 5 tests , H-2K b s AF BV421 5 µg , Histone H3 ps28 s HTA28 V45 5 tests , HLA-A,B,C G V45 5 tests , HLA-A2 BB7.2 V45 5 tests , BV421 1 tests , BV tests , BV51 5 tests , V45 5 tests , HLA-DR G46-6 V5 1 tests , V5 25 tests , I-A/I-E s 5/ BV65 25 tests , BV65 1 tests , BV421 5 µg , V5 5 µg , I-A b s AF BV421 5 µg , IFN-α2b 7N4-1 V45 5 tests , BV421 5 tests , BV65 5 tests , IFN-γ B27 V45 12 tests , V45 3 tests , V5 5 tests , s XG1.2 V45 5 µg , BV421 5 tests , G2-193 Ig, κ Light Chain V45 5 tests , s V45 5 µg , BV421 5 tests , IA6-2 BV51 5 tests , V45 5 tests , IgD V5 5 tests , BV51 5 µg ,* s 11-26c.2a BV65 5 µg ,* V45 5 µg , IgE s R35-72 BV51 5 µg ,* IgG G BV421 5 tests , V45 5 tests , 9

10 IgG1 s A85-1 Ig G2-127 s R6-6.2 BV421 5 µg , V45 5 µg , BV421 5 tests , BV51 5 tests ,* BV65 5 tests ,* V45 5 tests , BV421 5 µg , BV51 5 µg ,* V45 5 µg , IL-1 s JES5-16E3 V45 5 µg , IL-12 p4/p7 C11.5 V45 5 tests , s C15.6 V45 5 µg , IL-13 JES1-5A2 V45 5 tests , BV421 5 tests , N IL-17A V45 5 tests , s TC11-18H1 V45 5 µg , IL-17F O V45 1 tests , V45 25 tests , BV421 5 tests , BV51 5 tests ,* IL-2 V45 5 tests , s JES6-5H4 BV421 5 µg ,* V45 5 µg , IL-4 8D4-8 BV421 5 tests , V45 5 tests , s 11B11 BV421 5 µg , V45 5 µg , IL-6 Q2-13A5 V45 5 tests , s P5-2F3 V45 5 µg , ILT7 CD85g 17G1.2 BV421 5 tests , Invariant NK T-Cell 6B11 BV51 5 tests ,* BV711 5 tests ,* Ki-67 B56 V45 5 tests , KLRG1 s 2F1 BV421 5 µg , V45 5 µg , Lgr5 8F2 BV421 5 tests , Light Chain, λ JDC-12 BV421 5 tests , V45 5 tests , LPA-1 s DATK32 BV421 5 µg , Ly-49AB6 s A1 V45 5 µg , BV421 5 µg , Ly-6A/E s D7 V45 5 µg , V5 1 µg , V5 25 ug , BV421 5 µg , Ly-6C s AL-21 BV65 5 µg ,* V45 5 µg , BV65 5 µg , LY-6G s 1A8 V45 5 µg , BV421 5 µg , BV421 5 µg , Ly-6G and Ly-6C s RB6-8C5 BV51 5 µg , V45 1 µg , V45 25 µg , IP-1β D BV421 5 tests , V45 5 tests , Nestin Rat 25/NESTIN V45 5 tests , BV421 5 µg , NK-1.1 s PK136 BV51 5 µg , BV65 5 µg ,* V45 5 µg , Nkp46 9E2/Nkp46 V45 5 tests , Oct- 2, s 9A2 BV421 5 tests ,* p38 APK pt18/py182, s, Rat 36/p38 pt18/py182 PB 5 tests , Pax-5, s 1H9 BV51 5 tests , RORγt s Q BV421 5 µg , S6 ps235/ps236 N7-548 V45 5 tests , Siglec-F s E5-244 BV421 5 µg , Sox2, s O3-678 V45 5 tests , SSEA-1 s C48 BV421 5 tests , V45 5 tests , SSEA-3 C-631 also known as C631 BV421 5 tests , SSEA-4 C813-7 V45 5 tests , Stat1 py71, s 14/P-STAT1 PB 5 tests , Stat3 py75, s 4/P-STAT3 PB 5 tests , Stat5 py694 47/Stat5 py694 PB 5 tests , Stat6 py641 18/P-Stat6 V45 5 tests , Streptavidin BV421 1 µg BV51 1 µg BV65 1 µg BV711 1 µg V45 1 µg , V5 1 µg , T-bet, s O4-46 V45 5 tests , BV421 5 µg , TCR β Chain s H BV51 5 µg ,* BV65 5 µg , V45 5 µg , TCR γδ B1 BV421 5 µg , s GL3 BV421 5 µg , TCR Vα 2 s B2.1 BV421 5 µg , V45 5 µg , TCR Vβ 13 s R12-3 V45 5 µg , TER-119/ Erythroid cell s TER-119 V45 5 µg , V5 5 µg , TGF-β1 TW4-9E7 BV421 5 tests ,* BV421 5 tests , ab11 TNF V45 5 tests , s P6-XT22 V45 5 µg , BV421 5 tests , TRA-1-6 Antigen TRA-1-6 V45 5 tests , PB Pacific Blue *

11 BV421 5 µg , V45 1 µg , Ham IgG1, κ A19-3 BV51 5 µg , BV65 5 µg , BV711 5 µg , V45 1 µg , BV421 5 µg , Ham IgG1, λ1 G BV51 5 µg , BV711 5 µg , BV65 5 µg , BV421 5 µg , PB 1 µg , B81-3 Ham IgG2, κ V45 1 µg , V5 1 µg , Ha4/8 BV421 5 µg , Ham IgG2, λ1 Ha4/8 BV51 5 µg , BV65 5 µg , Ham Ig, λ1 G235-1 V45 1 µg , s Ig, κ BV421 5 µg , s IgA, κ V45 1 µg , BV421 5 µg , V45 1 µg , V5 1 µg , BV51 5 µg , X4 BV65 5 µg , s IgG1, κ BV65 5 µg , BV711 5 µg , BV786 5 µg , OPC-21 PB 1 µg , V45 1 µg , OPC-173 V45 1 µg , BV421 5 µg , s IgG2a, κ BV51 5 µg , G PB 1 µg , V45 1 µg , V5 1 µg , BV421 5 µg , BV51 5 µg , s IgG2b, κ V45 1 µg , V5 1 µg , BV711 5 µg , s IgG3, κ J66 V45 1 µg , BV421 5 µg , V45 1 µg , s Ig, κ G V5 1 µg , BV711 5 µg , BV51 5 µg , BV421 5 µg , BV65 5 µg , Rat IgG1, κ R3-34 BV711 5 µg , BV51 5 µg , V45 1 µg , BV421 5 µg , Rat IgG1, λ A11-1 BV51 5 µg , BV65 5 µg , V45 1 µg , BV421 5 µg , BV51 5 µg , BV65 5 µg , BV65 5 µg , Rat IgG2a, κ R35-95 BV711 5 µg , BV786 5 µg , PB 1 µg , V45 1 µg , V5 1 µg , BV421 5 µg , Rat IgG2a, λ B39-4 BV65 5 µg , V45 1 µg , Rat IgG2b, κ R35-38 BV421 5 µg , BV786 5 µg , A95-1 V45 1 µg , V5 1 µg , BV51 5 µg , Rat IgG2b, κ BV65 5 µg , R35-38 BV65 5 µg , BV711 5 µg , V45 1 µg , BV421 5 µg , Rat Ig, κ R4-22 BV51 5 µg , BV65 5 µg , BV711 5 µg , 7-AAD Staining Solution 2. ml , Fixable Viability Stain 45.1 mg , Violet Proliferation Dye mg , Transcription Factor Buffer Set 1 tests , 25 tests , man FoxP3 Buffer Set 1 tests , ouse Foxp3 Buffer Set 1 tests , Foxp3 BD Cytofix / Cytoperm Fixation / Permeabilization Solution Kit 25 tests , BD Cytofix / Cytoperm Plus Fixation / Permeabilization Solution Kit with BD GolgiStop 25 tests , Breferdin A BD Cytofix / Cytoperm Plus Fixation / Permeabilization Solution Kit with BD GolgiPlug 25 tests , onencin BD Cytofix / Cytoperm Fixation / Permeabilization Solution 125 ml , BD Cytofix Fixation Buffer 1 ml , BD Perm / Wash Perm/Wash Buffer 1 ml , Stain Buffer(FBS) 5 ml , Stain Buffer(BSA) 5 ml , BD PharmLyse Lysis Buffer(1X concentrate) 1 ml , 11

12 Brilliant Violet マルチカラー トレーニングキャンペーン BV 1 tech_cell@bd.com TEL Brilliant Violet T Sirigen Group Ltd. Cy T Amersham Bioscience Corp. Alexa Fluor olecular Probe Inc. BD BD Becton, Dickinson and Company 213 BD R

2 A2B, 0/A2B IgM AF647 0 T ,000 C3/AFP IgG Annexin V AF488 0 T ,000 PE 0 T ,000 APC 2 T ,000 FITC 2 T ,000 PE 2 T 60

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