Table 1 Isolation of legionellae from 24 hr-hot water bath without chlorination 1) Sampling from hot bath water used more than 5 days 2) Samplig from

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1 Key words : legionella, 24hr-hot water bath, chlorination, hot water contamination

2 Table 1 Isolation of legionellae from 24 hr-hot water bath without chlorination 1) Sampling from hot bath water used more than 5 days 2) Samplig from freshly prepared hot water.

3 Table 2 Evaluation of water quality of 24 hr-bath Smell grade : 0 ; no smell, 1 ; faint smell, 2 ; obvious smell, 3 ; bad smell Transparency grade: 1 ; 100%, 2 ; %, 3 ; %, 4 ; % Fig. 1 Detection of total bacteria and Legionella pneumophila from unchlorinated 24hr-hot water bathes. Five water bathes were selected for monitors. L. pneumophila not isolated from A and C water bathes during 2week-monitoring period.

4 李 766 Table One ml water Fig. 2 Change of heated water bath Calculated added 2ppm to a 24hr concentration not detected. of bacterial at 41 Ž at and 3 Survival of bacteria solution(1-3 ~105 then, concentration in a added to chlorination the mixed with 9 ml of hot-tab mixture. 塩素は約1時間は1ppmを維持したが,4時には検出されなくなった.レ concentration monitored. after CFU/ml) 41 Ž. water 娜他 bath of and After 間後ジオネラが検出され the 3hr, たE,B,Dの3つの浴槽に1日/回行った(Fig.3).次塩素濃度が2ppmにした.その塩素投与を亜塩酸ナトリウムは初期有効なるように投入し,モニターの結果レジオネラは翌日には検出されなくなり,6日間のモニター期間中毎日分離を試みたがレジオネラは分離されなかった.そ 3軒の浴槽のレジオネラは週1回4週の後この間モニターを継続したがレジオネラは検出されなかった. 塩素投与を2日の2軒間に1回とした場合をEとB の浴槽を使用して実験した(Fig.4) 前に4,000cfu/mlの.投与レジオネラが検出された一軒では1日後には検出されなくなったが1週過中4日目に検出された.一間の経方一般細菌は二日目感染症学雑誌第71巻第8号

5 Fig. 3 Chlorination of three water bathes every 24 hr. Growth of bacteria under 2ppm chlorination program ever 24hr. Before, numbers of bacteria before chlorination. Three legionellae-positive bathes were used in this study. Water samples were collected just before chlorination and total bacteria and Leginella were counted. Arrow indicates chlorination. L. pneumophila not detected from E, B and D water bathes even after one month. Fig. 4 Chlorination of two water bathes every 48 hr. Growth of bacteria under 2ppm chlorination program every 48hr. Before, numbers of isolated bacteria before chlorination. Water samples were collected just before chlorination and total bacteria and Legionella were counted. Arrow indicates chlorination.

6 2) Mashiba K, Hamamoto T, Torikai K : Center for Disease control : Update : Outbreak of Legionaires' disease associated with a crusie ship. JAMA 1994 ; 272 : ) Barvaree JM, Fields BS, Feeley JC, Gorman GW, Martin WT : Isolation of protozoa from water associated with a legionnellosis outbreak and demonstration of intracellular multi- plication of Legionella pneumophila. App Environ Microbiol 1986 ; 51 : ) Yamamoto H, Hashimoto Y, Eaki T : Comparison of detection methods for Legionella species in environmental water by colony isolation, fluorescent antibody staining and polymerase chain reaction. Microbiol Immunol 1993 : 37 : ) Ikedo M, Yabuuchi E : Ecological studies of Legionella species. Viable counts of Legionella pneumophila in cooling tower water. Microbiol Immunol 1986 ; 30 :

7 Isolation of Legionella pneumophila from 24 hr-home Bath Water and an Eradication Trial of the Bacteria from the Bath Na LI1), Tooru AOYAMA1), Hiroshi HORI2) & Takayuki EZAKI3) Research Institute of Environmental Microbes 2)Technology of Research Branch of Coronal Industories, Ltd. 3)Department of Microbiology, Gifu University of Medicine Contamination of 24 hr home bath with Legionella pneumophila is recently well recognized. Eradication of the water-bath contamination from L. pneumophila and other bacteria is an important matter to prevent the infection because the 24 hr-bathing facility is widely accepted in Japanese houses. Among the 16 bathing water samples we tested, Legionella pneumophia isolated from 6 cases (37.5%) when the bething water not treated with disinfectants. Number of L. pneumophila increased up to 103 cfu/ml and total culturable bacterial counts reached to 105 cfu/ml within 5 days when the water not treated. We selected 5 water bathes among 6 positive cases to study the bactericidal effect of chorine. As a result we concluded that the growth of L. pneumophila in 24 hr-water bath could be stopped by the 2 ppm chlorination program every day.

semen quality or those without WBC in semen. In the patients with azoospermia and normal FSH levels (normogonadotropic azzospermia), the antibody (IgG

semen quality or those without WBC in semen. In the patients with azoospermia and normal FSH levels (normogonadotropic azzospermia), the antibody (IgG CLINICAL STUDIES OF UROGENITAL INFECTIONS WITH CHLAMYDIA TRACHOMA TIS Report 2. The Epidemiology of Chlamydial Infections in Okayama District in Japan and Detection of Antibodies to Chlamydiae in the Sera