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5 ELISA, enzyme-linked immunosorbent assay; RAST, radioallergosorbent test; BHR, basophile histamine

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8 8) Tsuji H, Okada N, Yamanishi R, Bando N, Kimoto M, Ogawa T (1995) Measurement of Gly m BD 30 K, a major soybean allergen, in soybean products by sandwich enzyme-linked imunosorbent assay. Biosci Biotechnol Biochem 59 : ) Bush RK, Taylor SL, Nordlee JA, Busse WW (1985) Soybean oil is not allergenic to soybeansensitive individuals. J Alle Clin Immunol 76 : ) Crevel RWR, Kerfhoff MAT, Koning MMG (2000) Allergenicity of refined vegetable oils. Food Chem Toxicol 38 : ) Fremont S, Errahali Y, Bignol M, Metche M, Nicolas JP (2002) Allergenicity of oils. Alle Immunol (Paris) 34 : ) Fremont S, Errahali Y, Bignol M, Montagnon I, Metche M, Nicolas JP (2002) What about the allergenicity of vegetable oils? (2002). Internet Symp Food Alle 4 : ( ood-allergens. de). 13) Moneret-Vautrin DA, Hatahet R, Kanny G (1994) Risks of milk formulas containing peanut oil contaminated with peanut allergens in infants with atopic dermatitis. Pediatr Alle Immunol 5 : ) Moneret-Vautrin DA, Rance F, Kanny G, Olszewski A, Gueant JL, Dutau G, Guerin L (1998) Food allergy to peanut in France. Evaluation of 142 observations. Clin Exp Alle 28 : ) Zitouni N, Errahali Y, Metche M, Kanny G, Moneret-Vautrin DA, Nicolas JP, Fremont S (2000) Influence of refining steps on trace allergenic protein content in sunflower oil. J Alle Clin Immunol 106 : ) Errahali Y, Morisset M, Moneret-Vautrin DA, Kanny G, Metche M, Nicolas JP, Fremont S (2002) Allergen in soy oils. Allergy 57 : ) Ogawa T (2005) Soy Allergy. In: Soy in Health and Disease Prevention (Sugano M, ed), Taylor and Francis, Boca Raton. 18) Taylor SL, Hefle SL, Bindslev-Jensen C, Atkins FM, Andre C, Bruijnzeel-Koomen C, Burks AW, Bush RK, Ebihara M, Eigenman PA, Host A, Hourihane JOB, Isolauri E, Hill DJ, Knulst A, Lack G, Sampson HA, Moneret-Vautrin DA, Rance F, Vadas PA, Yungiuger JW, Zeiger RS, Salminen JW, Madsen C, Abbott P (2004) A concensus protocol for the determination of the threshold doses for allergenic foods: how much is too much? Clan Exp Alle 34 : ) Fukazawa T, Ishida S, Maruyama T, Yanagita T (2004) Determination of trace protein in oils and fats by florescence spectrophotometer. J Oleo Sci 53 : ) Foucard T, Edberg U, Malmheden Yman I (1997) Severe, even fetal, reactions to foodstuffs. Peanut and soya beans: underestimated allergens. Lakartidningen 94 : ) Moneret-Vautrin DA, Morisset M, Flabbee J, Kanny G, Kirch F, Parisot L (2002) Unusual soy oil allergy. Allergy 57 : ) Palm M, Moneret-Vautrin DA, Kanny G, Denery- Papini S, Fremont S (1999) Food allergy to egg and soy lecithins. Allergy 54 : ) Lavaud L, Perdu D, Prevost A, Vallerand H, Cossart C, Passemard F (1994) Baker's asthma related to soybean lecithin exposure. Allergy 49 : ) Andersen HL, Nissen I (1993) Presumed anaphylactic shock following Lipofundin infusion. Ugeskr Laeger 155 : ) Buchman AL, Ament ME (1991) Comparative hypersensitivity in intravenous lipid emulsions. J Parenterau Enteral Nutr 15 : ) Weidmann B, Lepique C, Heider A, Schmitz A, Niederle N (1997) Hypersensitivity reactions to parenteral lipid solutions. Suppo Care Cancer ) Awazuhara H, Kawai H, Baba M, Matsui T, Komiyama A (1998) Antigenicity of proteins in soy lecithin and soy oil in soybean allergy. Clin Exp Alle 28 : ) Gu X, Beardslee T, Zeece M, Sarath G, Markwell J (2001) Identification of IgE-biniding proteins in soy lecithin. Int Arch Alle Immunol 126 : ) Weangsripanaval T, Moriyama T, Kageura T, Ogawa T, Kawada T (2005) Dietary fat and an exogenous emulsifier increase the gastrointestinal absorption of a major soybean allergen, Guy m Bd 30K, in mice. J Nutr 135 : ) Cordle CT (2004) Soy protein allergy: incidence and relative severity. J Nutr 134 : ) Bush RK (2002) A new study of the allergenicity of soybean oil. FRI Newsuett 14(1) : 1. 32) Tattrie NH, Yaguchi M (1973) Protein content of various processed edible oils. J Inst Can Sci Technol Alime 6 : ) Porras 0, Carlsson B, Fallstrom SP, Hanson LA (1985) Detection of soy protein in soy lecithin, margarine and, occasionally, soy oil. Int Arch Alle Appl Immunol 78 : ) Klurfeld DM, Kritchevsky D (1987) Isolation and quantitation of lectins from vegetable oils. Lipids 22 : ) Doke S, Nakamura R, Torii S (1989) Allergenicity of food proteins interacted with oxidized lipids in soybean-sensitive individuals. Agric Biol Chem 53 : ) Chiu JT, Haydik TB (1991) Sesame seed oil anaphylaxis. J Alle Cuin Immunol 88 : ) Andersen HL, Nissen I (1993) Presumed anaphylactic shock after infusion of lipofundin. Ugekr Laeger 155 : ) Paschke A, Sunker K, Wigotzki M, Steinhart H (2001) Determination of the IgE-binding activity of soy lecithin and refined and non-refined soybean oils. J Chromato B Biomed Sci Appl 756 :

9 J Jpn Soc Nuts Food Sci 59 : (2006) Review Is Soybean Michihiro Oil Allergenic? Sugano*,1 (Received March 24, 2006 ; Accepted August 31, 2006) Summary : Soybean oil is ranked as one of the most allergenic soy foods. However, theoretical and circumstantial evidence suggests that the total protein content of edible soybean oil is clearly lower than the threshold level for inducing an allergic reaction. The reason for this discrepancy was therefore studied, and the findings suggested that the previously suggested possibilities that soybean oil contains either allergen proteins or hydroperoxides in sufficient amounts to cause or promote allergic reactions, or the abundance of linoleic acid in the oil as a cause, can all be dismissed. It seems likely that previous experiences with soybean oil produced in the past when the refining technology was unskilled have been unconsciously handed down. Therefore it is concluded that from a common sense viewpoint, soybean oil can no longer be regarded as a hazardous food, although care may be necessary with regard to the allergenicity of soybean lecithin preparations, because of its protein content and various uses. Key words : soybean oil, allergy, allergen, soy lecithin * Corresponding author ( 1 Professor Emeritus, Kyushu University and Prefectural University of Kumamoto, Najima, Higashi-ku, Fukuoka , Japan

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