术 资 1
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1 术 资 1
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10 MC1 MC1 BLAST CSK-1 FG4 M1-1 AB M1-2 AB M1-3 AB M1-4 AB M1-5 AB M1-6 AB M1-7 AB CUI zongjun et al. 24 hoers Environmental Science, 2002, 23(3): 36-39(EI) 10
11 MC1 Control Inoculated day 0 @ 11
12 ph days 12
13 MC1 Cellulose (100%) Residual straw (81%) Hemicellulose (97%) Lignin (51%) 13
14 PCS 400ml 50 4g 14
15 Days Cellulose (mg/l), Ethanol (mg/l) Acetate (mg/l), Lactate (mg/l) Acetate Glucose Glucose (mg/l) Lactate Cellulose Ethanol 1 0
16 MC1 16
17 DGGE MC1 Band Isolate Closest relative H G F M1-4 Virgibacillus pantothenticus M1-1 Bacillus licheniformis CSK-1 Clostridiumsp. FG4 Clostridium thermosuccinogenes E M1-6 Bordetella sp. B, C, D M1-5 Brevibacillus agri A M1-2,3,7 Pseudoxanthomonas sp. 17
18 DGGE 16S rdna band H isolate 4 Bacillus licheniformis isolate 1 B. subtilis B. pumilus B. circulan band C band D band B Bacillus brevis isolate 5 Brevibacillus agri Clostridium cellulovorans C. cellulolyticum C. thermocellum band G band F C. thermosuccinogenes Actinomyces bovis Myxococcus xanthus isolate 7 isolate 3 band A isolate 2 Pseudoxanthomonas broegbernens Stenotrophomonas maltophilia Alcaligenes faecalis Bordetella petrii isolate 6 band E 18
19 34 34 CSK-1 19
20 Cellulose-attaching microorganism Isolate CSK-1 attached to cellulose, and degraded cellulose under anaerobic condition Bacillus subtilis Clostridium termitidis Clostridium cellobioparum Clostridium papyrosolvens Clostridium cellulolyticum Bacteroides cellulosolvens Clostridium aldrichii 100 Acetivibrio cellulolyticus 100 Acetivibrio cellulosolvens CSK-1 Clostridium thermocellum 100 Clostridium thermosuccinogenes Clostridium stercorarium Clostridium thermolacticum Clostridium cellulosi Clostridium butyricum Clostridium leptum
21 21 Clostridium thermosuccinogenes F Utilization Acetate Glucose Cellobiose Cellulose Anaerobic growth Aerobic growth Closest relative Strain Bacillus licheniformis 1 Pseudoxanthomonas taiwanensis 3 Bordetella petri 6 Brevibacillus agri 5 Virgibacillus pantothenticus 4 Clostridium straminisolvens S MC1
22 CSK-1 Filter paper degradation (%) CSK1/Anaerobic CSK1/Aerobic No growth Community/Aerobic 8 day-cultivation 22
23 S S 1 0 S 3 68 S 4 0 S 5 58 S 6 53 S S F 71 S S
24 5 DGGE profile 24
25 Knockout communities Anaerobe Aerobe cellulolytic Non-cellulolytic S F Model community (SF356) Knockout community (S) 25
26 Filter paper degradation ph profile filter paper degradation (mg/ml) F 5 SF S 10 days ph days 8 10 S F SF
27 Oligosaccharides Acetate 1600 F concn. (mg/l) SF S days 3 5 SF356 6 F S days 27
28 4 Soluble saccharides Acetate Ethanol Lactate Acetate MC Cellulose (mg/l), Ethanol (mg/l) Acetate (mg/l), Lactate (mg/l) Glucose Glucose (mg/l) Lactate Cellulose Ethanol Days
29 * MC1 MC 8 * MC11 Clostridium straminisolvens CSK1 * MC1 * MC1 29
30 30
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独立栄養的炭酸固定代謝から紐解く古(いにしえ)
集団としての微生物の機能とその利用 微生物が集団で機能している環境 腸内 口腔 皮膚伝統的発酵食品製造酒類醸造有機物分解 ( 腐敗 コンポスト メタン生成など ) 廃水処理 微生物が集団で機能している環境 腸内 口腔 皮膚伝統的発酵食品製造酒類醸造有機物分解 ( 腐敗 コンポスト メタン生成など ) 廃水処理 腸内には約 100 兆個 重さにしておよそ 1kg もの細菌が少なくとも数百種類常在している
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12 20 10 14 00 12 43 2006 1 1994 1994 14 2 14 24 115 40 85 47 24 14 3 1990 17.4 47 8.1 1995 5.7 95 4 15 20 20 22 24 806 18 700 65 24 26 3,000 1990 2007 20 47 20 20 2007 28.2 5 1980 85 90 95 2000 1980 10
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80(4)70 80(4)70 852011 1 2 Cs Brown et al.,2008a 80(4)70 852011 3 H H C H H H C H H H C H O H O H O O O H H C H O O O H C O 4 2 + H 2 O HO + H + + pka=10.4 - HOCl HO/O- HOCl O - 3 - + O 3-5 131 1988 530200
3 JAS JAS JAS 4
1 2 3 JAS JAS JAS 4 5 6 7 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ph 102 106 K 31 32 13 3 15 79 33 34 35 36 37 20 6 H.22.3 http://www.caa.go.jp/foods/pdf/syokuhin19.pdf 38