Corning Cell Culture Surfaces
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1 Corig Cell Culture Surfaces
2 Corig...2 Corig BioCoat...3 Corig PureCoat...8 ECM Mimetic...8 Corig PureCoat ECM Mimetic...8 Corig Corig Sythemax Corig Corig Corig PureCoat Corig Primaria Corig CellBIND i vitro i vitro Deis Kukel Microscopy, Ic.
3 100 PYREX Matrigel BioCoat ECM mimetic peptide Corig Falco Corig ECM extracellular matrix i vivo Corig Matrigel Corig BioCoat 2 ECM Mimetic Corig ECM Mimetic PureCoat ECM Mimetic Corig
4 ISO9001 Corig Corig Egelbreth-Holm-Swarm EHSEHS IV / i vitro i vivo w i vivo Xeograft w ES/iPS w w i vivo 25 Corig / LDEV/LDHV LDEV PCR 3 Corig LDEV/LDHV ScietificSupportJP@corig.com Corig i vitro T4-2 β -catei- GM130- gree- ES H9 Corig HUVEC-2 Corig 2
5 Corig BioCoat cgmp BioCoat Corig Falco BioCoat I BioCoat I 24 Corig Getest BioCoat BioCoat NG-108 / Corig HC * GFR ES i vivo GFR GFR Stem Cell Techologies mtesr 1 ES/iPS 8-12 mg/ml mg/ml 8-12 mg/ml 8-12 mg/ml / ml ml ml ml ml ml GFR ml ml ml HC ml ml ml HC ml GFR ml BioCoat mm 60 mm 100 mm 24 * HC High Cocetratio 3
6 Corig - D - - L - RGD i situ 0.15 M NaCl 1 mm CaCl mm CAPS ph 11.0 dh 2 O 1 mg/ml 10 mm ph 7.7 dh 2 O ph DPBS µ g/ml dh 2 O 5% mg/ml / mg mg µg µg mg mg mg BioCoat mm 60 mm 100 mm 150 mm mm 4 8 T-25 T-75 T-150 T mm 60 mm 100 mm 150 mm 12 mm 35 mm 4 8 T-25 T-75 T-150 T mm 60 mm 12 mm 4
7 Corig Corig PuraMatrix I I I II i vivo i vivo 1% w/v ph mg/mL 20 mm ph N 3-4 mg/ml 0.02 N 8-11 mg/ml 2 mm 2-4 mg/ml 15 M 3-4 mg/ml / ml mg mg mg mg mg mg BioCoat mm 60 mm 100 mm 150 mm T-25 T-75 T-150 T mm 4 8 5
8 Corig III IV IV V VI V ed-to-ed Egelbreth-Holm- Swarm 10 mm mg/ml 10 mm mg/ml 0.05 M mg/ml 10 mm mg/ml 1 M 1.25 mm Tris mg/ml ph / mg mg mg mg mg BioCoat mm 60 mm 100 mm T-25 T-75 T mm T-75 6
9 Corig / - - / - - / / Corig Matrigel ES ips Corig BioCoat PDL / BioCoat PLO/ BioCoat / Egelbreth-Holm- Swarm Egelbreth-Holm- Swarm Egelbreth-Holm- Swarm -D- Egelbreth- Holm- Swarm EHS -L- Egelbreth- Holm- Swarm EHS Egelbreth-Holm- Swarm EHS 0.05 M Tris-HCl 0.15 M NaCl mg/ml ph M Tris-HCl 0.15 M NaCl mg/ml ph M Tris-HCl 0.15 M NaCl mg/ml ph / mg mg mg BioCoat mm 60 mm 100 mm 150 mm T-25 T mm 12 mm
10 Corig PureCoat ECM Mimetic Corig ECM Mimetic cgmp Corig ECM Mimetic cgmp ISO Corig ECM Mimetic I Corig Sythemax Falco Corig CellSTACK Corig ECM Mimetic Corig PureCoat ECM Mimetic Corig PureCoat 2 PureCoat ECM Mimetic w Corig PureCoat ECM Mimetic RGD α -5 w Corig PureCoat ECM Mimetic I α -2 I Corig PureCoat ECM Mimetic I I ECM 8
11 Corig PureCoat 5 10 Corig PureCoat ECM Mimetic Corig PureCoat ECM Mimetic 5 CD73 CD90 CD105 CD34, 45, 11b, 19, HLA DR Fibroecti mimetic Corig PureCoat ECM Mimetic CD73 CD90 CD105 CD34 CD45 CD11b CD19 HLA-DR ECM Corig -521 BioLamia -521 Corig -521 α 5 β 2 γ 1-521hESC ipsc hpsc -521 ROCK PSCs hpsc -521 ES / Oct-4 DAPI 9
12 Corig PureCoat Corig Sythemax Corig Sythemax RGD Sythemax mtesr mtesr1 Corig Sythemax II- 5 ips Oct-4 Corig Sythemax H7 ES Nkx2.5 α - ECM Mimetic Corig PureCoat ECM Mimetic Corig PureCoat ECM Mimetic I Corig Sythemax Corig -521 ready-to-use I ready-to-use ROCK RGD GFOGOR I RGD SF XF AF XF XF XF XF AF SF XF XF XF SF SF SF XF XF / mg µg µg mg mg 6 24 T-75 T Corig CellSTACK T-75 T * SF = XF = AF = 10
13 Corig PureCoat Corig Corig ready-to-use Corig Corig TRAP Corig AW264.7 Corig Ultra-Low Attachmet Corig Corig CellSTACK TC 384 HT Corig Stripwell mm 100 mm T-25 T-75 Corig CellSTACK 1 11
14 Corig PureCoat Corig PureCoat Corig Primaria Corig Primaria X ESCA Corig CellBIND Corig PureCoat Corig PureCoat Corig Primaria Corig CellBIND / / Falco mm T-75 T-175 Falco mm T-75 T-175 Falco mm 60 mm 100 mm T-25 T-75 Corig mm 60 mm 100 mm T-25 T-75 T-150 T-175 T-225 Corig HYPERFlask Corig CellSTACK Corig HYPERStack Corig Corig 12
15 I IV - PDL, PLL PDL/ P-L / PuraMatrix PureCoat ECM Mimetic PureCoat ECM Mimetic I Sythemax -521 CellBIND PureCoat PureCoat ECM ECM Mimetics Aortic edothelial cells, BAEC Bile duct cells (epithelial) Boe marrow cells (boe resorptio, osteoclast) Brai microvessel (edothelial) Cardiomyocytes; cardiac (edothelium, progeitor cells) Coloocytes (epithelial) Dorsal root gaglia Embryoic cortical euros Embryoic sympathetic euros Edothelial cells; edothelial coloy formig cells Erythrocyte culture (parasite developmet stages [asexual, sexual]) Hepatocytes Hippocampal euros Huma periodotium (periodotal ligamet) Huma osteoclast precursors (osteoclast, pit formatio) HUVEC (edothelial) HVSMC Keratiocytes Mammary epithelial cells; breast cells (lumial, myoepithelial ad edothelial) Microvascular, BME (edothelial) Mouse spleic T-cells Muscle cells, myoblasts, myogeic cells, myotubes Neuroal cells (cortical, cerebellar graule, astrocytes, sesory, sympathetic) Oligodedrocytes (glial; precursors) Osteoblasts Pacreatic islet, eoatal (3- to 5-day-old) rat islets of lagerhas Parotid aciar cells Peripheral blood moouclear cells Postatal mouse vestibular gaglio euros Schwa cells (glial) Sertoli cells (spermogeic) Skeletal muscle cells (myocytes, myotubes) Smooth muscle cells (edothelial, aortic, vascular) Urothelial cells Valvular iterstitial cells Zygote ad blastocyst developmet stages 13
16 I IV - PDL, PLL PDL/ P-L / PuraMatrix PureCoat ECM Mimetic PureCoat ECM Mimetic I Sythemax -521 CellBIND PureCoat PureCoat ECM ECM Mimetics ARH-77 (lymphoblast) BHK-21 (fibroblast) Breast cacer cells (established cell lies) C2C12 (myoblast) Cell immobilizatio (Gi-1, Nasal epithelial cells, Molt-4 ad K562 huma leukemia cells, Sf9 Cells) Chiook Salmo Embryo Cells (CHSE-214) CHO, CHO-1, CHO-K1 (epithelial, edothelial, trasfected fusio protei) COS-7 (fibroblast, trasfected) Dorsal Root Gaglia (trasfected) H1299 (trasfected-huma o-small cell lug carcioma cell lie) HEK-293 (trasfected, epithelial), EcoPack2-293, HEK-SRAtet cells, Livig Colors HEK-ZsGree proteasome sesor (trasfected) HeLa HepG2 (hepatocyte), Hep3B (hepatoma) HT-1080 (epithelial) hfob 1.19, MG63 (osteoblast cell lies) Huma MOLT-4, drosophila S2 (biomaterial ad tissue egieerig applicatios) Keratiocytes (huma eoatal) L929 (fibroblast, trasfected) LCAP (prostate cacer cell lie) MCF7 (epithelial) MCF-10A (epithelial) MDA-MB-231 MDA-MB 435 MM41 (skeletal myoblasts, trasfected) MRC5 N2AB-1 (euroblastoma) NIH/3T3, 3T3 (fibroblast) PC-3, PC-12 RTG-2 (raibow trout goad cells) RAW (macrophage; osteoclast differetiatio, pit formatio) SH-SY5Y SK-MEL-28 U266 (lymphoblast) U937 (moocyte) Vero cells 14
17 I IV - PDL, PLL PDL/ P-L / PuraMatrix PureCoat ECM Mimetic PureCoat ECM Mimetic I Sythemax -521 CellBIND PureCoat PureCoat ECM ECM Mimetics Huma embryoic stem cell (hesc) Huma iduced pluripotet stem cell (hipsc) hmscs (boe marrow derived, adipose derived) Huma retial progeitor cells (RPE) resc; rat edothelial progeitor cells Neuroal stem cell (itestial/eteric) i vitro ECM ECM Mimetics hesc (cerebral orgaoid model) hesc (pacreatic) hesc, hipsc (cardiomyocytes) hesc, hipsc, mesc (Germ Cell Layers: ectoderm, mesoderm, edoderm; hematopoietic progeitor; defiitive differetiatio; cardiomyocytes) hesc, hipsc, mesc, mipsc (edothelial) hesc, hipsc (itestial orgaoids) hesc, hipsc (euroal) hesc (osteogeic) hesc, hipsc (smooth muscle) hesc, mesc (lug epithelial) hesc, mesc, resc (hepatocyte, hepatocyte-like) Huma NPCs (differetiatio to euroal cells) hpscs, mpscs (real progeitor cells, real tubular cells, edoderm) mesc (hematopoietic) mesc, Chicke (cardiomyocytes) mesc, resc, mipsc (euroal, progeitor) mpscs (ier ear sesory epithelia) hesc, hipsc (retial pigmet epithelial) 15
18 i vitro I IV - PDL, PLL PDL/ P-L / PuraMatrix PureCoat ECM Mimetic PureCoat ECM Mimetic I Sythemax -521 CellBIND PureCoat PureCoat ECM ECM Mimetics hadscs; adipose (edothelial) Cardiac progeitor cells (cardiomyocyte) Colo (epithelial orgaoids) Hair follicle (melaocytes, euros, smooth muscle) Hepatic progeitor cells (hepatic, biliary cells) Itestial (orgaoids, crypt-villus) Keratiocytes (epidermal) Lug (sphere) Mammary epithelial cells MSC (cardiomyocyte, chodrocyte, hematopoietic, hepatocyte, euro, osteocyte, spheroid) MSC (edothelial progeitors) Muscle (skeletal) Neural progeitor/stem cells (euro, astrocytes, euroblast) Pacreatic (edocrie) Preatal rat cells (euro, glial cells) Retial (retial euro) Salivary glad Stomach (gastric uits) 16
19 I IV - PDL, PLL PDL/ P-L / PuraMatrix PureCoat ECM Mimetic PureCoat ECM Mimetic I Sythemax -521 CellBIND PureCoat PureCoat ECM ECM Mimetics 4T1 (mouse breast cacer cell lie) Cardiac fibroblast Hep3B (hepatoma; toxicity/drug screeig) MCF-7 (epithelial) MCF-10A (epithelial) MDA-MB-231 MDA-MB-361 HeLa HT-1080 (epithelial) hesc, Rat (edothelium) Huma melaoma cell lies SBCL2 (RGP), WM-115, (VGP) ad 451-LU (MM) ad keratiocytes (spheroid model) Mouse embryoic pacreatic progeitors (orgaoid) MSCs, Ovaria cacer cells (OCC) Primary rat hepatocytes Rat hepatocyte progeitor cells (spheroid) SK-MEL-28 cells MEFs (stromal fibroblast) U266 (lymphoblast) Surface selectio guide NCBI Web Referece 17
20 Begiig-to-ed Solutios for Cell Culture At Corig, cells are i our culture. I our cotiuous efforts to improve efficiecies ad develop ew tools ad techologies for life sciece researchers, we have scietists workig i Corig R&D labs across the globe, doig what you do every day. From seedig starter cultures to expadig cells for assays, our techical experts uderstad your challeges ad your icreased eed for more reliable cells ad cellular material. It is this expertise, plus a 160-year history of Corig iovatio ad maufactur ig excellece, that puts us i a uique positio to offer a begiig-to-ed portfolio of high-quality, reliable cell culture cosumables. Corig CORNING Icorporated For a listig of trademarks, visit us at All other trademarks are the property of their respective owers Tel Fax CLSJP@corig.com Tel ScietificSupportJP@corig.com 2018 Corig Icorporated CLS R B
150903_Corning Cell Culture Surfaces.indd
Corning Cell Culture Surfaces ....................................................... 1.................................................. 2 Corning.................. 2 Corning BioCoat....................................................
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