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1 1, 1, 1, 1, 1, 1,2, 1,2, 1 1 DDBJ 2 AJACS :20-15:

2 DDBJ DDBJ DDBJ

3

4 DDBJ NCBI (GenBank) DDBJ EBI (EMBL-Bank) GEO DDBJ Omics ARchive(DOR) ArrayExpress DTA (DDBJ Trace Archive) DRA (DDBJ Read Archive)

5 DDBJ Omics Archive DRA Submitter 1 ( ) NCBI EBI DDBJ GEO AE DOR SRA ERA DRA

6 DDBJ Read Annota8on Pipeline Image data Instrumentation data Sequence + quality (fastq) >Seq1 AGTCGGGTGG.... base calling mass-ftp DRA [email protected] MiGAP() RNA-Seq () Plant genome annotations () SNP annotation pipelines NIG Contigs (Overlapping reads) Scaffolds (Supercontigs) Complete genome - Annotation De novo Assembly finishing/gap closure annotation Reference Genome Mapping + Contig + + mass-ftp MSS + Annotation

7 FASTQ Base Call FASTQ format Q-score (evaluation measure of base Sequence +ID GTCTCGACCAGCCTCGACAACCTC +ID49_20708_20H04AAXX_R1:7:300:39:401 hhuhhhhhyhhhhhhhehcaa`bskhh\xh Ref. Q score (Phred) ASCII dec 0 : : h ASCII Glyph Solexa (illumina 1.3 format) Q20 Q15

8 DDBJ PC Cluster Master node 100TB Slave nodes 33 nodes CPU Memory Time Maximum Job# Computer nodes 2*3.16GHz 4GB 168H 42

9 1

10 (Quality Score/Coverage ) OUTPUT EVALUATION STATISTICS Pipeline start DRA sequence file FASTQ format quality score coverage mapping tools common alignment file depth SAM/BAM format error rate mapped ratio maximum contig size assembly tools common sequence file FASTA format N50 contig size

11 DDBJ DOR DDBJ Manual Annotation Pipeline Tag Counting Structural, Functional Annotations Download results via FTP server Genome Mapping de novo Assembly FASTQ + metadata DRA or TRA(temporal) MSS formatted file

12 USER 1. data upload 2. pipeline ID DDBJ Read Archive 5. registration 4. analytical results 3. data analysis DDBJ Read Annotation Pipeline DRA / MSS formatted files DDBJ Mass Submission System 5. registration

13 DDBJ

14 DDBJ Read Annotation Pipeline User ID: guest ( )

15 FASTQREAD 1. List of data Download and view of DRA metadata 2.Select query files 3. Go to next

16 1. Select map or assembly 3. Go to next 2. Select a tool

17 1. Select files for a query set 2. Confirmation 3. Go to next

18 1. Specify preset, or download/upload 2. Specify an INSD accession number to retrieve data from DDBJ DB 3. Go to next

19 1. Go to next

20 1. Run 2. Go to status page

21 1. Screen login user 2. Goto Download page

22 A. Download evaluations B. Download output files

23 Viewer (Tablet) 1. Tablet

24 Mapping 1. ReferenceMapping.sam

25 reference 1. Mapping(.sam) 2.Reference

26 Mapping viewer 1.

27 Jun Mashima Toshihisa Okido Asami Nozaki Hitoshi Kunii Daisuke Ikumi Takeshi Konno Nobuhiro Hoshi Shouta Morizaki Acknowledgements Yoshio Tateno DDBJ Annotators, members DRA is part of the National project of integrating life science databases, and is supported by the Japan Science and Technology Agency Institute for Bioinformatics Research and Development.

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