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1
2 DNA
3 Plasmodium (P. falciparum) (P. vivax) (P. malariae) P. ovale 4 40%
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9 5.0 mg 2.5 g 10mM ph ml 1) 2) B nm / 520nm 400-1,000 3) 2. OptiMAL R FLOW NOW R ICT Malaria P.f./P.v. Binax OptiMAL R FLOW : Plasmodium falciparum lactate dehydrogenase - 7 -
10 15-20 NOW R ICT Malaria P.f./P.v. Binax : OptiMAL R HRP-2 histidine-rich protein 2 3. DNA PCR : PCR PCR DNA MPH MPH 18S rrna 18S rrna DNA DNA - PCR PCR PCR 13 /10 l - 8 -
11 312 MPH DNA DNA P.f. P.v. P.o. P.o.-v P.m P.f.: P.v.: P.m.: P.o. : P.o.-v: variant 18S rrna 1) 104 l 2) 4 IASR Vol.22: ; 18S rrna Pf Pv Pm Po J. Clin Microbiol. 33: ,
12 in vitro WHO Le Bras WHO WHO/WPRO, United Nations Avenue, P.O. Box 2932, 1000 Manila, Philippines 96 WHO WHO in vitro Well A mol/l/blood B mol/l/blood C mol/l/blood D mol/l/blood E mol/l/blood F mol/l/blood G mol/l/blood H mol/l/blood *SP = / 80:1 Drug sensitivity tests in malaria parasites
13 24 N 2 90%; CO 2 5%; O 2 5% 5ml 5mL EDTA parasitemia RPMI1640(-) RPMI g NaHCO g HEPES 5.95 g 950 ml 1N NaOH ph7.4 1,000 ml RPMI1640(+) RPMI1640(-) 10 25µg/ml
14 µM µM 1. (0.50µM) 20µL 2. 5% 500µL µm µm 25 20µL 5 500µL g 1,500rpm 3. RPMI g 1,500rpm RPMI % % 5 5% µM
15 11. 50% 100% IC 50 IC 100 IC µM IC µM µM WHO IC µM 0.16µ M 4. WHO IC 50 40nM FCR-3 SGE-1 IC 50 IC 100 1) DNA DNA ( ) 1994, ) DNA 191(1), 67-73, ) Rieckmann KH, Sax LJ, Campbell GH, Mrema JE : Drug sensitivity of Plasmodium falciparum -An in vitro microtechnique, Lancet, 7, 22-23, ) Le Bras J, Deloron P : In vitro study of drug sensitivity of Plasmodium falciparum - Evaluation of a new semi-micro test, Am. J. Trop. Med. Hyg., 32(3), , ) In vitro Test for susceptibility of P. falciparum to chloroquine and mefloquine. Annex 6 (Bruce-Chwatt LJ, Black RH, Canfield CJ, Clyde DF, Peters W, Wernsdorfer WH. Eds) in Chemotherapy of Malaria. Revised second edition. World Health Organization monograph series no. 27, Geneva: World Health Organization ) Werndorfer WH and Payne D : Drug sensitivity tests in malaria parasites, in
16 Malaria Vol. 2 Principles and Practice of Malariology (Werndorfer WH and McGregor M eds.) pp , (Churchill Livingstone, London) 7) Inaba H, Ohmae H, Kano S, Faarado L, Boaz L, Leafasia J, Suzuki M : Variation of incubation time in an in vitro drug susceptibility test of Plasmodium falciparum isolates studied in the Solomon Islands, Parasitology International, 50, 9-13,
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Summary
Summary Itiswell-knownthatpeopleare infected with malariavia mosquitos called Anopheles spp. The present paper describes the distribution of malaria on earth, historyofthedisease, biology, lifecycle, syndrome,
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