[PDF] 2010年度 冬のラボ充実キャンペーン カタログPDF

Transcription

1 PD-10 Glutathione Sepharose 20 20% % OFF 10 10% % OFF Vivaspin % OFFF 20% OFF 5 NanoVue Plus ÄKTAprime plus

2 ライフサイエンス事業のあゆみ ライフサイエンスは 遺伝子 タンパク質 細胞レベルの基礎研究領域のみならず 医薬品の研究 開発や バイオ医薬品の製造におよぶ広範なバイオテクノロジー領域において 統合的な技術基盤を提供します 私た ちの技術基盤とは 機器 試薬 解析ソフトウェアに加えてそれらのシステムを有効に機能させるための技術 支援やアプリケーションの提供 機器のアフターサポートが統合されたトータルソリューションを意味し 単なる 物 の販売とは一線を画するものです 複雑な実験の連続から構成される研究の成功にはこれらが必須条件であ ることを私たちは実績を通じてお客様とともに学んできました 2010 ÄKTA avant 150 Biacore GE ヘルスケア ジャパン誕生 富士フイルムとグローバル提携 設立 VP-DSC 発売 VP-Cpillary DSC 発売 itc 200 発売 Auto-iTC 200 発売 1900 James Whatman が Whatman ブランド 製紙工場を設立 設立 Wove を 開発 ミニユニ FTA カード DNA サンプルの保存 精製用製品を発表 1993 Cyclopore を買収 Pharmacia Biosensor 社 設立 2006 Biacore 社設立 BIAcore を 発表 発売 D-DIGE 技術製品化 1943 LKB-Produkter 世界最初の Tiselius が 電気泳動法 を開発 Continuous UV モニター Uvicord l を発表 Tiselius が電気泳動法開発の 業績によりノーベル賞受賞 1986 設立 FPLC System Mono Q Mono S Mono P を発表 デキストランの発見 Porath がゲルろ過の現象を発見 Tiselius が Gel filtration の名称を提案 Sephadex を開発 1892 GE Affinity Chromatography の技術を発表 1879 W.D. クーリッジが 高真空熱陰極 X 線管 クーリッジ管 を発明 1

3 GE & Science Prize for Young Life Scientists GE Science GE & Science Prize for Young Life Scientists prize 2009 Regional Winner Q Q Grand Hotel Q & A Q

4 GE & Science Prize for Young Life Scientists 2 ÄKTAdesign 5 ÄKTAprime PC & 6 ÄKTAdesign UV 6 7 ÄKTAdesignBiacore OFF & HiTrap OFF 9 11 GST www.gelifesciences.co.jp

5 INDEX GST DNA OFF 10%OFF Mag Sepharose BMB

6 ÄKTAdesign ÄKTAprime Biacore ÄKTAdesign Biacore ÄKTAdesign ÄKTAdesign UNICORN Ver.4 UNICORN 5.20 P-960 Frac-950 Windows XP / Vista PC PC PC UNICORN 5.20 upgrade CU 950 UNICORN UNICORN 5.00 UNICORN OS Windows XP TEL UNICORN 5 FDA 21 CFR Part 11 P-950 A-900 UNICORN 5www.gelifesciences.co.jp UNICORN 4.1 UNICORN 5.2 P-950 P-950 P-960 A-900 A-900 A UV UV-900 P MPa A UV2UV3 3 1 UNICORN 1 UNICORN 2 UNICORN Main Menu Help About UNICORN 15 OFF 324, ,000

7 ÄKTA Prime PC & ÄKTAprime Rec-112 Prime View UV 1 PC PrimeView 5.00 PC PC (Windows XP) PrimeView 5.00 upgrade OA ÄKTAprime plusäktaprime AKTAprime Upgrade Kit P-950, purge TEL OFF 688, ,000 UV ÄKTAexplorer or ÄKTApurifier Check Lamp Intensity 210mm 85% 300nm 95% CheckCheck Lamp Intensity 20% Check Lamp Run Time 200h Check Check Lamp Run Time 4,000 2,000 4,000 2,000 Lamp Intensity 20 Lamp Run Time 4,000h 2,000h UV ÄKTApurifier UPC 20 OFF 253, ,000

8 ÄKTAprime or Biacore 10% % OFF PC 1 3 ÄKTAprime 2005 ÄKTAprime plus ÄKTAprime ÄKTAprime ÄKTAprime plus 10%OFF 10%OFF ÄKTAprime 160, ,000 Biacore X , ,000 Biacore T , ,000 Biacore , ,000 Biacore , ,000 Biacore A100 1,100, ,000 Biacore J 360, ,000 Biacore Q 412, ,000 Biacore X 400, ,000 ÄKTAprime plus 7www.gelifesciences.co.jp

9 & HiTrap OFF 9 20th anniversary ÄKTA or OFF GST

10 &20 HiTrap OFF HiTrap HiTrap 18 20% OFF 2 HiTrap th anniversary HiTrap HiTrap HiTrap HiTrap HiTrap 1 HiTrap HiTrap 1 HiTrap 2 Web 3 Web HiTrap 9www.gelifesciences.co.jp

11 & HiTrap 18 HisTrap HP HiTrap Protein G HP ml 1 5 ml 3 His-tag HiTrap 1 ml 1 Ni 2+ Ni Sepharose High Performance 40 mg His-tag /ml 1 Protein G HiTrap 5 ml 1 Protein G 25 mg IgG/ml HiTrap NHS-activated HP 5 1 ml 20 HiTrap NHS 10 μmol NHS/ml 4 HiTrap Q HP 5 1 ml 5 HiTrap Protein G HP 5 1 ml 6 HiTrap Q HP 5 5 ml 7 HiTrap Desalting 5 5 ml 8 HiTrap Heparin HP 5 1 ml Q Sepharose High Performance 50 mg HSA/ml Protein G 25 mg IgG/ml Q Sepharose High Performance 50 mg HSA/ml Sephadex G-25 Superfine ml heparin 3 mg antithrombin III /ml 9 HisTrap HP 5 5 ml 10 HiTrap NHS-activated HP 1 5 ml 11 HiTrap Protein G HP 2 1 ml 12 HiTrap rprotein A FF 1 5 ml 13 HiTrap SP HP 5 1 ml Ni Sepharose High Performance 40 mg His-tag /ml 1 14 HiTrap Heparin HP 1 5 ml NHS 10 μmol NHS/ml 15 HisTrap HP 1 5 ml Protein G 25 mg IgG/ml 16 HisTrap FF 5 1 ml Protein A 50 mg IgG/ml 17 HiTrap SP HP 5 5 ml SP Sepharose High Performance 55 mg ribonuclease 18 HiTrap Chelating HP 5 1 ml heparin 3 mg antithrombin III /ml Ni Sepharose High Performance 40 mg His-tag /ml 1 Ni Sepharose 6 Fast Flow 40 mg His-tag /ml 1 SP Sepharose High Performance 55 mg ribonuclease Iminodiacetic acid μmol Cu 2+ /ml 143 kda (His) 6 -tagged protein 2 20%OFF %OFF HisTrap HP 5 1 ml ,500 14,800/ HiTrap Protein G HP 1 5 ml ,500 47,600/ HiTrap NHS-activated HP 5 1 ml ,000 12,000/ HiTrap Q HP 5 1 ml ,000 12,000/ HiTrap Protein G HP 5 1 ml ,000 53,600/ HiTrap Q HP 5 5 ml ,000 25,600/ HiTrap Desalting 5 5 ml ,700 23,760/ HiTrap Heparin HP 5 1 ml ,000 12,000/ HisTrap HP 5 5 ml ,000 60,800/ HiTrap NHS-activated HP 1 5 ml ,500 10,800/ HiTrap Protein G HP 2 1 ml ,500 22,000/ HiTrap rprotein A FF 1 5 ml ,000 48,800/ HiTrap SP HP 5 1 ml ,000 12,000/ HiTrap Heparin HP 1 5 ml ,500 10,800/ HisTrap HP 1 5 ml ,600 16,480/ HisTrap FF 5 1 ml ,500 13,200/ HiTrap SP HP 5 5 ml ,000 25,600/ HiTrap Chelating HP 5 1 ml ,000 12,000/ 2 2

12 &ÄKTA or ÄKTA or GE OFF OFF 50 5 OFF OFF Superdex 75 10/300 GL ,000 Superdex /300 GL ,000 Superdex Peptide 10/300 GL ,000 Superdex 200 5/150 GL ,000 Superdex 75 5/150GL ,000 Superose 6 10/300 GL ,000 Superose 12 10/300 GL ,000 HiLoad 16/60 Superdex 75 pg ,000 HiLoad 16/60 Superdex 200 pg ,000 HiLoad 16/60 Superdex 30 pg ,000 HiLoad 26/60 Superdex 75 pg ,000 HiLoad 26/60 Superdex 200 pg ,000 HiLoad 26/60 Superdex 30 pg ,000 HiPrep 16/60 Sephacryl S-100 HR ,900 HiPrep 16/60 Sephacryl S-200 HR ,900 HiPrep 16/60 Sephacryl S-300 HR ,900 HiPrep 26/60 Sephacryl S-100 HR ,000 HiPrep 26/60 Sephacryl S-200 HR ,000 HiPrep 26/60 Sephacryl S-300 HR ,000 HiPrep 26/10 Desalting ,600 HiPrep 26/10 Desalting ,000 Mono Q 5/50 GL ,000 Mono Q 10/100 GL ,000 Mono Q HR 16/ ,590,000 Mono Q 4.6/100 PE ,000 Mono S 5/50 GL ,000 Mono S 10/100 GL ,000 Mono S HR 16/ ,590,000 Mono S 4.6/100 PE ,000 Mono P 5/50 GL ,000 Mono P 5/200 GL ,000 RESOURCE DUO 72-AS ,000 RESOURCE Q 1 ml ,000 RESOURCE Q 6 ml ,000 SOURCE 15Q 4.6/100 PE ,000 RESOURCE S 1 ml ,000 RESOURCE S 6 ml ,000 SOURCE 15S 4.6/100 PE ,000 HiLoad 16/10 Q Sepharose HP ,000 80!! HiLoad 26/10 Q Sepharose HP ,000 HiLoad 16/10 SP Sepharose HP ,000 HiLoad 26/10 SP Sepharose HP ,000 HiPrep Q FF 16/ ,000 HiPrep Q XL 16/ ,100 HiPrep SP FF 16/ ,000 HiPrep SP XL 16/ ,100 HiPrep DEAE FF 16/ ,000 HiPrep CM FF 16/ ,000 RESOURCE ETH 1 ml ,100 RESOURCE ISO 1 ml ,100 RESOURCE PHE 1 ml ,100 RESOURCE HIC ,000 SOURCE 15PHE 4.6/100 PE ,000 HiLoad 16/10 Phenyl Sepharose HP ,000 HiLoad 26/10 Phenyl Sepharose HP ,000 HiPrep Phenyl FF (low sub) 16/ ,000 HiPrep Phenyl FF (high sub) 16/ ,000 HiPrep Butyl FF 16/ ,000 HiPrep Octyl FF 16/ ,000 GSTPrep FF 16/ ,000 HisPrep FF 16/ ,600 HiPrep IMAC FF 16/ ,300 HiPrep Heparin FF 16/ ,100 HiScreen HiScreen Capto Q ,500 HiScreen Capto S ,500 HiScreen Capto MMC ,700 HiScreen Capto adhere ,700 HiScreen Capto DEAE ,500 HiScreen MabSelect ,400 HiScreen MabSelect Xtra ,400 HiScreen MabSelect SuRe ,700 HiScreen Butyl FF ,500 HiScreen Butyl-S FF ,500 HiScreen Octyl FF ,500 HiScreen Phenyl FF (high sub) ,500 HiScreen Phenyl FF (low sub) ,500 11www.gelifesciences.co.jp

13 & GST Glutathione Sepharose 4B PD-10 GST 35 ml GST GraviTrap New 1. Equilibrate 2. Load sample 3. Wash 4. Elute Glutathione Sepharose 4B PD ml LabMate PD-10 Buffer reservoir ml 1 PBS 2 3 PBS ml 600 μl GST SpinTrap Glutathione Sepharose 4B GST μl μl 30 GST Buffer Kit GST SpinTrapGST MultiTrap FFGST MultiTrap 4BGST GraviTrapGSTrap FFGSTrap 4BGSTrap HP 10 PBS, ph mm Tris-HCl, ph ml g 50 ml 27 Glutathione Sepharose 4B GST 20%OFF GST GraviTrap ,000 71,200 GST SpinTrap ,500 26,000 GST Buffer Kit ,000 28,000

14 &No.1 2-D Protein Extraction Buffer Trial Kit 6 2-D Protein Extraction Buffer Trial Kit II VI VI 2-D Protein Extraction Buffer Trial Kit 100 mg 2-D Protein Extraction Buffer Trial Kit Web 2-D Protein Extraction Buffer Trial Kit I VI 13www.gelifesciences.co.jp

15 & No.2 IgG 60 IgG IgG HiTrap Albumin and IgG Depletion Albumin & IgG Depletion SpinTrap IgG A B IgG A B IgG A HiTrap Albumin and IgG Depletion Albumin & IgG Depletion SpinTrap SpinTrap HiTrap Albumin & IgG Depletion ml μl %OFF 2-D Protein Extraction Buffer Trial Kit 6 for 10 ml ,000 20,000 2-D Protein Extraction Buffer I 1 for 50 ml ,000 24,000 2-D Protein Extraction Buffer II 1 for 50 ml ,000 32,000 2-D Protein Extraction Buffer III 1 for 50 ml ,000 32,000 2-D Protein Extraction Buffer IV 1 for 50 ml ,000 32,000 2-D Protein Extraction Buffer V 1 for 50 ml ,000 32,000 2-D Protein Extraction Buffer VI 1 for 50 ml ,000 32,000 Albumin & IgG Depletion SpinTrap ,500 49,200 HiTrap Albumin & IgG Depletion 2 1 ml ,000 67,200 Albumin & IgG Removal KitRPN6300 Albumin & IgG Removal Kit Web

16 & DNARNA A2500 Yield (µg) B5 A 9 B Mammalian Protein Extraction Buffer RIPA Buffer carbonic anhydrase 50 mm Tris-HCl ph mm NaCl1% NP-400.1% Nadeoxycholate 4 Relative activity MPEB RIPA Mammalian Protein Extraction Buffer Mammalian Protein Extraction Buffer Zymolase A1200 Yield (µg) B 25 Relative activity S. cerevisiae P. pastoris Yeast Protein Extraction Kit Glass beads and RIPA S. cerevisiae P. pastoris A50 mg 2 B alkaline phosphatase Glass beads RIPA Yeast Protein Extraction Buffer Kit 15www.gelifesciences.co.jp

17 & Yeast Protein Extraction Buffer Kit Yeast Suspension Buffer Zymolyase Yeast Protein Extraction Buffer DNARNA DNAtotal RNA 1 illustra tripleprep Kit DNAtotal RNA PCR 2-D DIGE illustra tripleprep Kit DNA purification RNA purification Protein isolation 1. Sample homogenization and lysis 2. DNA binding Flowthrough 3. DNA wash 1 6. RNA binding Flowthrough 4. DNA wash 2 7. DNase (optional) 10. Protein precipitation 5. DNA elution 8. RNA wash 11. Protein wash Lysis 5 30 min 9. RNA elution 12. Protein resuspension DNA purification <10 min RNA purification <10 or 20 min Protein isolation <30 min 20%OFF 1 Yeast Protein Extraction Buffer Kit /-20 25,000 20, ml Mammalian Protein Extraction Buffer 500 ml ,000 32,000 illustra tripleprep Kit /4 63,000 50,400

18 & Vivaspin MWCO Vivaspin % 5 70% Vivaspin 500 Vivaspin 2 Vivaspin 6 Vivaspin 20 12,900 24,300 26,300 18,300 20% OFF 10,400 19,500 21,100 14, µl ml 2 6 ml 5 20 ml 3, , , , , , www.gelifesciences.co.jp

19 & Klari-Flex New 90 mm 500, 1000 ml OFF 0.22 µm PES 250 ml ,000 13, µm PES 500 ml ,000 20, µm PES 1000 ml ,000 25, µm PES 250 ml ,000 13, µm PES 500 ml ,000 20, µm PES 1000 ml ,000 25, ,000 50, , ,000

20 OFF 10 OFF % % OFF 1 10% % OFF ECL Plus Western Blotting Detection Reagents for 1,000 cm 2 membrane PreScission Protease 500 units GST NEW mm Prepacked Disposable PD-10 Columns Glutathione Sepharose 4B 10 ml GST Full-Range Rainbow Molecular Weight Markers 250 µl ECL Western Blotting Detection Reagents for 4,000 cm 2 membrane www.gelifesciences.co.jp

21 8 6 Ficoll-Paque PLUS ml ExoSAP-IT 100 PCR Thrombin 500 units GST NEW Hyperfilm ECL18 24 cm 50 sheets ECL Advance Western Blotting Detection Kit NEW Hyperfilm ECL cm 100 sheets Protein G Sepharose 4 Fast Flow 5 ml ECL Western Blotting Detection Reagents for 2,000 cm 2 membrane Anti-Rabbit IgG, HRP-Linked Whole Ab Donkey 1 ml ECL HRP NEW Hybond-P30 cm 3 m 1 roll PVDF NEW 3MM Chr cm

22 ECL ECL 20 ECLECL PlusECL Advance Web Mag Sepharose 39 Vivaspin 17 Full-Range Rainbow Molecular Weight Markers Google scholar "Rainbow Molecular Weight Markers" ECL ECL ECL DualVue Western Blotting Markers 3 7 S-tagged ECL 21www.gelifesciences.co.jp

23 3MM Chr cm 18 HRP Anti-Rabbit IgG, 16 HRP-Linked Whole Ab Donkey 16 PVDF Hybond-P ,310 PVDF Google scholar"hybond-p" ECL / ECL Plus / ECL Advance HRP 18 1 ECL Plus Western Blotting Detection Reagents 1 27,300 ECL Plus ECL Google scholar "ECL Plus" ECL Western Blotting Detection Reagents 7 for 4,000 cm 2 membrane 15 for 2,000 cm 2 membrane ECL Hyperfilm ECL cm sheets sheets 8,720 ECLECL PlusECL AdvanceCDP-Star Google scholar "Hyperfilm ECL" ECL Advance Western Blotting Detection Kit 12 1,700 ECL Google scholar "ECL Advance"

24 NAME ECL Plus ECL ECL Advance ECL Plex ECL Plus ECL ECL Advance ECL Plex 18 D E T E C T I O N B L O T T I N G E L E C T R O P H O R E S I S A N T I B O D Y ECL P.34 P.34 P.35 P.35 P.35 P.36 P.36 P.37 P / SDS ECL DualVue Western Blotting MarkersRainbow Molecular Weight Markers 52 ECL DualVue Western Blotting MarkersRainbow Molecular Weight Markers SDS 72 PVDF HRP 36 HRP P.37 Tween X 5 1 Web Web Web LAS ImageQuant LAS ImageQuant LAS ,875,600 9,200, ImageQuant LAS 4010 ECL Plus PC SE260 TE77 PWR PC ImageQuant TL Web ImageQuant LAS X X ImageQuant LAS 4010 ImageQuant LAS 4010 X 1/2 3.1 µg ImageQuant LAS 4010 Tray Position160 secsensitivityhigh Hyperfilm-ECLX 60 secimagescanner III Transferrin ng Transferrin ng 23www.gelifesciences.co.jp

25 Web Web Protein Science Club Web PSC %OFF %OFF Full-Range Rainbow Molecular Weight Markers 250 µl RPN800E ,800 15,840/ Anti-Rabbit IgG, HRP-Linked Whole Ab Donkey 2 1 ml NA934-1ML 4 46,500 37,200/ Hyperfilm ECL18 24 cm 100 sheets ,600 38,880/ Hyperfilm ECL18 24 cm 50 sheets ,100 24,080/ Hybond-P30 cm 3 m 1 roll RPN303F 30,900 24,720/ 3MM Chr cm ,800 25,440/ %OFF 10%OFF Low-Range Rainbow Molecular Weight Markers 250 µl RPN755E ,000 16,200 High-Range Rainbow Molecular Weight Markers 250 µl RPN756E ,000 16,200 ECL DualVue Western Blotting Markers 25 gel loadings RPN ,500 18,500 Anti-Mouse IgG, HRP-Linked Whole Ab Sheep µl NA UL 4 8,200 7,400 Anti-Mouse IgG, HRP-Linked Fab2 Fragment Sheep 2 1 ml NA9310-1ML 4 51,600 46,500 Anti-Rabbit IgG, HRP-Linked Whole Ab Donkey µl NA UL 4 8,200 7,400 Anti-Rabbit IgG, HRP-Linked Fab2 Fragment Donkey 2 1 ml NA9340-1ML 4 51,600 46,500 Anti-Rat IgG, HRP-Linked Whole Ab Goat 2 1 ml NA ,500 41,900 Anti-Rat IgG, HRP-Linked Fab2 Fragment Goat 2 1 ml NA ,100 47,800 Anti-Mouse IgG, HRP-Linked Whole Ab Sheep 3 1 ml NXA931-1ML 4 46,500 41,900 Anti-Mouse IgG, HRP-Linked Whole Ab Sheep 2 1 ml NA931-1ML 4 46,500 41,900 Hybond-P20 20 cm 10 sheets RPN2020F 22,800 20,600 Hybond-P14 16 cm 15 sheets RPN1416F 20,100 18,100 Hyperfilm ECL5 7 inches 50 sheets ,800 25,100 Hyperfilm ECL8 10 inches 50 sheets ,100 27,100 Hyperfilm ECL8 10 inches 100 sheets ,900 45,900 Hyperfilm ECL24 30 cm 50 sheets ,900 45,900 Hyperfilm ECL35 43 cm 50 sheets ,000 66,600 2 IgG IgG IgM, IgE IgA IgG 3

26 18 ECL Western Blotting Detection Reagents ECL ECL ECL RI EIAELISA Amerlite HRP RI P 32 P 1980 dutp DIG 5 25www.gelifesciences.co.jp P DIG 1987 Amerlite Amerlite ECL Amerlite Amerlite RIA X Amerlite HRP EMBL

27 SN Enhanced Chemiluminescence ECL Direct ECL Direct 1989 ECL Direct 125I ECL Direct I ECL ECL ECL ECL Direct 1990 ECL 1990 ECL ECL ECL PCR ECL Professor Larry KrickaUniversity of Pennsylvania John Osborn Sian Godwin Karsten Fjarstedt Ale:ke Danielsson Catherine Howat Dr David Fernie Val Jones Penny Owen Dr Ian Hesslewood Linsey Cresswell Dr Mark Briggs Clifford Smith Martin Cunningham Jan Turner Lynne Goodman Caroline Kelly LLC Associates Ltd Linsey Cresswell LLC Associates Ltd Dr Ian Durrant Oxford Immunotec Ltd Dr Richard Cumming Ithaka Life Sciences Web ECL 18

28 18 GST 18 3 GST GST Klari-Flex 18 GST HRP GST ELISA GST 96-Well Detection Module 96 GST GST Detection Module GST 1-chloro-2,4-dinitrobenzene (CDNB) pgex GST 340 nm GST Vivaspin 17 GST Glutathione Sepharose 4B 5 16,100 4 GST PD-10 Empty Columns Google scholar"glutathione Sepharose 4B" GST PreScission Protease 2 3, GST Google scholar "PreScission Protease" GST Thrombin mg GST 10 units Thrombin GST Factor Xa Factor Xa pgex Vector pgex-3xpgex-5x-1pgex-5x-2pgex- 5X-3 GST 27www.gelifesciences.co.jp

29 18 Recombinant Protein Purification Handbook Principles and Methods GST ,000 40,000 GST His-tag Web Q&A ThrombinPreScission Protease Web QA Web 2 20%OFF %OFF Glutathione Sepharose 4B 10 ml ,500 21,200/ PreScission Protease 500 units ,000 18,400/ Thrombin 500 units ,400 8,320/ 2 10%OFF 10%OFF Glutathione Sepharose 4B 100 ml , ,500 Glutathione Sepharose 4B 3 10 ml ,000 55,800 Glutathione Sepharose 4B 5 10 ml ,000 85,500 GST 96-Well Detection Module 5 plates ,500 72,500 GST Detection Module ,400 78,700 Glutathione Sepharose 4 Fast Flow 25 ml ,000 84,600 Glutathione Sepharose High Performance 25 ml , ,600 Factor Xa 400 units ,000 94,500

30 18 A) GSTrap 4B C) Glutathione Sepharose Glutathione Sepharose Glutathione Sepharose 4 FF Glutathione Sepharose 4B GST Glutathione Sepharose 4 FF GST-His 6 (dimer 52 kda) GST-Hippocalsin (dimer 90 kda) GST-GFP-His 6 (dimer 110 kda) GST-Fibrinogen (dimer 110 kda) Protein GST-His 6 26 kda (Dimer 52) GST-Hippocalsin 45 kda (Dimer 90) GST-GFP-His 6 55 kda (Dimer 110) GST-Fibrinogen 55 kda (Dimer 110) Yield (%) Concentration (mg/ml) Low Load GST-tagged protein Concentration mg/ml Total load mg B) GSTrap FF GST-His 6 (dimer 52 kda) GST-Hippocalsin (dimer 90 kda) GST-GFP-His 6 (dimer 110 kda) GST-Fibrinogen (dimer 110 kda) Yield (%) Concentration (mg/ml) All purifications were performed with the following protocol: Columns: GSTrap 4B and GSTrap FF, 1 ml Binding/wash buffer: 10 mm sodium phosphate, 140 mm sodium chloride, 2.7 mm potassium chloride, ph 7.4 Elution buffer: 10 mm sodium phosphate, 140 mm sodium chloride, 2.7 mm potassium chloride, 10 mm glutathione, ph 7.4 Flow rates: Binding: 0.3 ml/min Equilibration: 1 ml/min System: ÄKTAexplorer 10 Detection: Absorbance, 280 nm, SDS-PAGE, Coomassie staining and Deep Purple staining Mass Spectrometry AGlutathione Sepharose 4B GSTrap 4B BGlutathione Sepharose 4 FF GSTrap FF C Glutathione Sepharose Characteristics Glutathione Sepharose Glutathione Glutathione High Performance Sepharose 4 Fast Flow Sepharose 4B Matrix Highly cross-linked Highly cross-linked 4% agarose 6% agarose 4% agarose Average 34 μm 90 μm 90 μm particle size Ligand mg glutathione/ml μmol glutathione/ml μmol concentration medium (based on Gly) medium glutathione/g washed and dried medium Binding > 10 mg recombinant > 10 mg recombinant > 5 mg recombinant capacity 1 glutathione glutathione glutathione S-transferase/ml medium S-transferase/ml medium S-transferase/ml medium Recommended < 150 cm/h cm/h < 75 cm/h flow rate 2 Chemical Stable to all commonly Stable to all commonly Stable to all commonly stability used aqueous buffers, e.g. used aqueous buffers, e.g. used aqueous buffers. 1 M acetate, ph 4.0 and 1 M acetate, ph 4.0, and Exposure to 0.1 M NaOH, 6 M guanidine 6 M guanidine hydrochloride 70% ethanol, or 6 M hydrochloride for 1 h for 1 h at room temperature guanidine hydrochloride at room temperature for 2 h at room temperature or to 1% (w/v) SDS for 14 d causes no significant loss of activity. ph stability Storage 4 C 30 C 4 C 30 C 4 C 8 C temperature Storage buffer 20% ethanol 20% ethanol 20% ethanol 1 The binding of GST-tagged proteins depends on size, conformation, and concentration of the protein in the sample loaded. Binding of GST to glutathione is also flow dependent, and lower flow rates often increase the binding capacity. This is important during sample loading. Protein characteristics, ph, and temperature, but also the media used may affect the binding capacity. 2 H 2 O at room temperature. 29www.gelifesciences.co.jp

31 Lens Cleaning Tissue mm %OFF %OFF mm ,200 7,360/ Whatman Paper DNA 1733 I

32 18 Prepacked Disposable PD-10 Columns 4 Prepacked Disposable PD-10 Columns PD-10 / Prepacked Disposable PD-10 Columns 4 44,800 PD Kathon Sephadex G-25Medium 8.3 ml 2.5 ml 5,000 1,000 PD-10 Spin Adapter Google scholar "PD-10" Prepacked Disposable PD-10 Columns 4 Spin Adaptor PD-10 Columns 4 PD ml PD-10 Column LabMate buffer reservoirs PD-10 Columns 25 ml LabMate buffer reservoirs Spin Adaptor PD-10PD Trap PD-10 Sephadex 96 PD-10 Sephadex G PD -10 P D Midi Tr ap G- 25 PD Mini Tr ap G- 25 PD Sp in Tr ap G- 25 PD Mult it ra p G- 25 PD Mini Tr ap G Sephadex G- 25 Sephade x G- 25 Sephadex G- 25 Sephadex G- 25 Sephadex G- 25 Sephadex G well / Mr 5, 000 5, 000 5, 000 5, 000 5, ml 8. 5 ml 5 ml 80 0 µl 80 0 µl 5 ml 8. 3 ml ml 2. 1 ml 50 0 µl 50 0 µl 2. 1 ml ml ml ml µl µl µl ml ml ml % % % % % % 90 % 90 % 90 % 85 % 85 % 75 % PD Midi Tr ap G Sephadex G ml 5.3 ml ml % 75 % 31www.gelifesciences.co.jp

33 Web PD-10 Web PD10 18 Vivaspin 17 PCR MicroSpin G-25 Columns Mini Dialysis Kit sample float 2 20%OFF %OFF Prepacked Disposable PD-10 Columns ,700 31,760/ %OFF 10%OFF PD-10 Empty Columns ,000 18,000 LabMate PD-10 Buffer reservoir ,700 4,300 PD SpinTrap G ,100 31,600 PD MultiTrap G well plates ,700 83,500 PD MiniTrap G ,300 39,000 PD MidiTrap G ,300 39,000 PD MiniTrap G ,300 39,000 PD MidiTrap G ,500 46,400 Collection plates 96 well, 500 ul, V-shaped bottom ,900 3,600 Mini Dialysis Kit 1 kda cut-off, up to 250 µl ,500 39,200 Mini Dialysis Kit 1 kda cut-off, up to 2 ml ,500 39,200 Mini Dialysis Kit 8 kda cut-off, up to 250 µl ,500 39,200 Mini Dialysis Kit 8 kda cut-off, up to 2 ml ,500 39,200 MicroSpin G-25 Columns ,500 24,800 2PD MultiTrap 96 Collection plates

34 18 Ficoll-Paque PLUS 8 Ficoll-Paque PLUS Ficoll-Paque PLUS Ficoll-Paque PLUS 8 8 4, g/ml 0.12 EU/ml T B Google scholar "Ficoll-Paque PLUS" Ficoll-Paque PLUS 1 10 EDTA 24 Ficoll-Paque PLUS 4 20% FicollPercoll Web Ficoll-Paque PREMIUM GMP ISO 13485:2003 Ficoll-Paque PLUS Regulatory Support File 1 1 Regulatory Support File Percoll PLUS / Percoll mg/ml 2 20%OFF %OFF Ficoll-Paque PLUS ml ,000 28,800/ %OFF 10%OFF Ficoll-Paque PLUS ml , ,500 Ficoll-Paque PREMIUM ml ,000 36,000 Ficoll-Paque PREMIUM ml , ,500 Ficoll-Paque PREMIUM ml ,000 36,000 Ficoll-Paque PREMIUM ml ,000 36,000 Ficoll PM g ,000 22,500 Ficoll PM g ,000 90,000 Percoll 250 ml ,000 18,900 Percoll 1 L ,000 38,700 Percoll PLUS 250 ml ,000 21,600 Percoll PLUS 1 L ,000 54,900 33www.gelifesciences.co.jp

35 DNA DNA DNA DNA ExoSAP-IT PCR ExoSAP-IT 9 3,260 PCR PCR 80 Google scholar "ExoSAP-IT" DNA illustra TempliPhi DNA Amplification Kit DNA 15 DYEnamic ET Terminator Cycle Sequencing Kit 9 Thermo Sequenase II GC 18 AutoSeq G-50 4 Web exosap Web Nucleic Acid Sample Preparation for Downstream Analyses Principles and Methods ,000 DNA DNARNA DNARNA 2 20%OFF %OFF ExoSAP-IT 100 US ,200 19,360/ %OFF 10%OFF ExoSAP-IT 500 US ,000 96,800 ExoSAP-IT 2,000 US , ,300 illustra TempliPhi 100 Amplification Kit 100 reactions ,300 48,000 illustra TempliPhi 500 Amplification Kit 500 reactions , ,900 DYEnamic ET Terminator Cycle Sequencing Kit templates US ,400 56,200 DYEnamic ET Terminator Cycle Sequencing Kit 2 1,000 templates US , ,800 AutoSeq G ,800 21,500 AutoSeq G ,900 75,600 AutoSeq G-50 1, , ,300 2Macintosh ABI Model 373A/373SABI PRISM 377/310/377-XL Windows NT/2000 ABI PRISM /3100-Avant /3730xl ABI 3130POP7

36 18 Protein G Sepharose 4 Fast Flow 14 Protein G Sepharose 4 Fast Flow Lab Packs 14 1, Protein G IgG Protein A IgG Google scholar "Protein G Sepharose 4 Fast Flow" nprotein A Sepharose 4 Fast Flow 599 Google scholar "Protein A Sepharose 4 Fast Flow" Protein A/G Protein G Protein A Protein A Protein G Protein G/A Immunoprecipitation Starter Pack Web Protein A/G Sepharose HiTrap MAbTrap Kit Protein G or Spin Protein G HP SpinTrap Protein A HP SpinTrap Protein A/G HP SpinTrap Buffer Kit 2 20%OFF %OFF Protein G Sepharose 4 Fast Flow 5 ml ,500 50,800/ 2 10%OFF 10%OFF Protein G Sepharose 4 Fast Flow 25 ml , ,300 Protein G HP SpinTrap ,300 21,900 Protein A/G HP SpinTrap Buffer Kit ,200 21,800 MAbTrap Kit ,500 24,800 Immunoprecipitation Starter Pack ,500 34,700 nprotein A Sepharose 4 Fast Flow 5 ml ,000 49,500 nprotein A Sepharose 4 Fast Flow 25 ml , ,500 Protein A HP SpinTrap ,300 19,200 35www.gelifesciences.co.jp

37 NanoVue Plus & NEW UP NanoVue Plus with Printer PC PC USB : mm W * D H : 4.5 kg µl 2 µl 200 1,100 nm nm A10 mm 5 nm 2 nm 0.5 nm DNA UV NanoVue 280 nm 260 nm 200 nm 300 nm NanoVue 1 NanoVue Plus with Printer NanoVue Plus with Printer 1 1,700,000 Nucleic Acid Sample Preparation for Downstream Analyses Principles and Methods ,000 1,700,000 Spare Paper for Printer 20 rolls ,800 NanoVue Plus with BluetoothNanoVue Plus with SD Card

38 GeneQuant 100 GeneQuant µl 45 mm mm GeneQuant mm GeneQuant 1300 GeneQuant PC 1 2 : mm W D H4.5 kg 5.0 µl GeneQuant ,100 nmgenequant nm A 5 nm 2 nm 1 nm GeneQuant 1300 UV Bradford BCA Lowry Biuret GeneQuant 100 UV Bradford BCA Lowry Biuret GeneQuant 1300 CyDye Tm GeneQuant 1300 with Printer classic GeneQuant 1300 with Printer classic , mm ,100 GeneQuant 100 with Printer classic GeneQuant 100 with Printer classic , mm ,100 GeneQuant 1300 with Bluetooth GeneQuant 100 classic www.gelifesciences.co.jp 900, ,000

39 ÄKTAprime plus ÄKTAprime plus His-tag W400 D450 H530 mm13 kg PC MPa10 bar145 psi90 W mm W D H13 kg ml/min 10 cp 10 ml/min5 cp 10 ml/min ÄKTAprime plus QÄKTAprime PrimeViewPC A PrimeView ÄKTAprime PrimeView UV Q A PC ÄKTAprime PC ÄKTAprime RS-232C 1.8 m 5 m Tel ÄKTAprime plus PrimeViewPC ÄKTAprime plus PrimeViewPC 1 1,800,000 1,800, nm UV/ 100 l500 l2 ml5 ml GE

40 39www.gelifesciences.co.jp Mag Sepharose GE Mag Sepharose Sepharose His-tag 50 mg/ml medium His Mag Sepharose Ni IgG 27 mg/ml medium Protein A/G Mag Sepharose Xtra Mag Sepharose Sepharose LC-MS New Sepharose His-tag Sepharose His Mag Sepharose NiGE = His-tag 20 mm 20 mm Web Mag Sepharose

41 His Mag Sepharose Ni 72-AS01-11 His Mag Sepharose Ni His Buffer Kit MagRack 6 Protein A Mag Sepharose Xtra 72-AS01-06 Protein A Mag Sepharose Xtra Ab Buffer Kit His Mag Sepharose Ni Protein A/G Mag Sepharose Xtra Web His-tag His Mag Sepharose Ni New 2 1 ml ,000 20,000 His Mag Sepharose Ni New 5 1 ml ,500 45,000 His Mag Sepharose Ni New 10 1 ml ,900 77,000 His Mag Sepharose Ni 1 New 1 72-AS ,000 50,000 His Mag Sepharose Ni 1 New 1 72-AS ,000 77,000 Protein A Mag Sepharose Xtra New 2 1 ml ,500 25,000 Protein A Mag Sepharose Xtra New 5 1 ml ,000 55,000 Protein G Mag Sepharose Xtra New 2 1 ml ,500 25,000 Protein G Mag Sepharose Xtra New 5 1 ml ,000 55,000 Protein A Mag Sepharose Xtra 1 New 1 72-AS ,000 65,000 Protein G Mag Sepharose Xtra 1 New 1 72-AS ,000 65,000 Protein A/G Mag Sepharose Xtra 2 New 1 72-AS ,000 60,000 Protein A/G Mag Sepharose Xtra 2 New 1 72-AS ,000 87,000 Protein A Mag Sepharose µl ,800 16,000 Protein A Mag Sepharose µl ,000 50,000 Protein G Mag Sepharose µl ,800 16,000 Protein G Mag Sepharose µl ,000 50,000 NHS NHS Mag Sepharose µl ,800 16,000 NHS Mag Sepharose µl ,000 50,000 TiO 2 Mag Sepharose µl ,600 12,000 TiO 2 Mag Sepharose µl ,200 44,000 1Mag Sepharose = 5 1 ml 2Mag Sepharose = 2 1 mlprotein A Mag Sepharose Xtra Protein G Mag Sepharose Xtra MagRack ,000 His Buffer Kit ,200 Ab Buffer Kit ,700 Protein A/G HP SpinTrap Buffer Kit ,800 NHS HP SpinTrap Buffer kit ,

42 NEW BioDirect Mail GE Technical Tips Technical Tips Pure Protein Club Downstream Protein Sciences Club Cellular Sciences ips/es Web Web GE Healthcare vol.2 GE vol.2 Web INDEX ÄKTAmicro 56 IN Cell Analyzer www.gelifesciences.co.jp

43 BMB GE BMB Web GE , ECL BMB Web

44 TEL FAX TEL FAX Q&AScience Program Web CGP