gtf Wnt5a-Ror2 IL-8 in vitro

Ozony Tron P. gingivalis SOD ELISA Location of main occluding areas and masticatory ability in patients with reduced occlusal support

12-Methacryloyloxydodecylpyridinium bromide MDPB MDPB PB PB EPMA Streptococcus mutans PB WPB WPB WPB EPMA WPB PB FS WPB PB FS

Streptococcus mutans nm WPB MDPB MDPB WPB EPMA WPB Streptococcus mutans WPB

Poly P Poly P Ca Poly P Poly P MC3T3-E 1 ALP p-nitrophenyl phosphate substrate, ph10.5 Fluoresceinisothiocyanate FITC annexin V propidium iodide PI flow cytometory FCM total RNA reverse transcriptase-polymerase chain reaction RT-PCR Poly P polyethyleneimine thin layer chromatography PEI-TLC plate MC3T3-E1 Poly P Pi mmol/l MC3T3-E1 mmol/l Poly P ALP MC3T3-E1 mmol/l Poly P FCM Poly P

PI annexin V Poly P MC3T3-E1 osteocalcin β- Poly P MC3T3-E1 Poly P PEI-TLC Poly P Poly PPi PPi Pi3 Pi4 MC3T3-E1 β- Poly P Poly P Emdogain p Poly P Poly P Poly P Poly P Poly P Poly P Pi PPi Pi3 Poly P in vivo Poly P Poly P Poly P Poly P MC3T3-E1 in vivo in vitro

gtf CSI dft CSI dftcsi Real-time PCR S. mutans S.sobrinus gtf gtf gtf B gtf C gtf D

gtf I S. mutans gtf B D gtf B D S. sobrinus S. mutans gtf I gtf S. mutans S. sobrinus gtf B gtf C gtf D dft CSI dftcsi Real-time PCR S. mutans S. sobrinus S. mutans gtf B gtf D gtf

Wnt5a-Ror2 TNFα IL-1 Wnt5a Wnt5a Ror2 Wnt5a RT-PCR D Wnt5a GST-soluble Ror2 GST-sRor2 GST Ror2 Wnt5a Mφ M-CSF RANKL TRAP HE Ror2 Wnt5a GST-sRor2

GST Wnt5a Ror2 Mφ Wnt5a Mφ M-CSF RANKL Mφ Wnt5a Ror2 Wnt5a Wnt5a Wnt5a-Ror2 Wnt 5a Ror2 GST-sRor2 GST-sRor2 Wnt Wnt5a Ror2 Wnt5a Ror2 Wnt5a Ror2 RT-PCR Wnt5a Ror2 Ror Ror2 GST-sRor2Wnt5a Wnt5a Ror2 Wn5a-Ror2 Ror2 Wnt5a-Ror2 Wnt

Wnt5a Ror2 Ror2 Wnt5a Wnt5a-Ror2

IL-8 AZM in vitro AZM IL -8 IL-8 TNF-α AZM AZM in vitro MMP EM AZM JOM Porphyromonas gingivalis LPS ng/ml IL-6 IL-8 PGE ELISA µg/ml IL-6 IL-8 PGE MMP

AMZ LPS IL-8 EM JOM LPS IL-8 LPS IL-6 PGE MMP-2 MMP-9 AZM LPS IL-8 ERK JNK p38mapk PKA PI3K PLCγ NF-κB IL-8 NF-κB PGE AZM LPS IL-8 in vitro PGEIL-6 IL-8 AZM LPS IL-8 AZM IL-8 IL-8 IL-6 IL-8 PGE

1.8-cineol 1.8-cineol 1.8-cineol mg/ml Candida albicans JCM 1842 Klebsiella pneumoniae JCM 1662 Prevotella intermedia ATCC 25611 Prevotella nigrescens ATCC 33563 Actinobacillus actinomycetemcomitans JCM 8577 Porphyromonas gingivalis ATCC 49417 Streptococcus mutans Ingbritt Streptococcus sobrinus 6715 Streptococcus salivarius JCM 5707 1.8-cineol 1.8-cineol Streptococcus salivarius

1.8-cineol 1.8-cineol 1.8-cineol

µm

R SS SEP g SEP P N P -N µ P -N P -N

SEP SEP SEP

AIC χ Fisher s exact probability test

cdna PCR in situ ISH real time PCR myelin basic protein MBP MBP

ISH MBP MBP real time PCR myelin basic protein MBP PCR real time PCR in situ

in vitro PG EIL-6 IL-8 in vitro Porphyromonas gingivalis LPS ng/ml PGEIL-6 IL-8 ELISA MEK DMSO LPS COX -2 - mg/ml COX COX Inhibitor Screening Assay Kit COX Inhibitor Screening Assay Kit AcPLA ERK ERK LPS COX R http : //www.r-project.org/

LPS PGE LPS PGE LPS IL-6 IL-8 COX-1 COX-2 LPS COX-2 mg/ml LPS cpla ERK cpla MEK LPS PGE MEK LPS IL-6 IL-8 LPS PGE LPS PGE ERK ERK cpla PGE LPS in vitro PGEIL-6 IL-8 ERK cplapge in vitro

-L-

VF VE QOL VE VE VE VF

Wallenberg VF

BMP in vitro in vivo

R SS SEP A SEP P - N A A

P -N A P -N P -N P -N P -N A P -N P -N A A SEP A

A A

A Ca bfgf bfgf in vitro Gin-1-1 WST-1 Gin-1 / µm DMSO bfgf ELISA Gin-1 mm bfgf Student t p

Gin-1 bfgf in vitro in vitro Gin-1

Candida albicans C. albicans JCM1542 MCZ NCCLS MCZ MIC80 MCZ RPMI µg/ml MCZ µl GC mmφmm stroke / CFU/ml C. albicans µl SCDLP C. albicans µg/ml MCZ MCZ MIC -µg/ml RPMI1640 - µg/ml

MIC MCZ C. albicans µg/ml µg/ml - MCZ MCZ

Ozony Tron Ozony Tron Ozony Tron

Ozony Tron Ozony Tron

P. gingivalis SOD ELISA SOD Porphyromonas gingivalis P. g. SOD SOD Fe-SODMn -SOD P. g. SOD Amano, A J Bacteriol 172 : 1457-63, 1990 P. gingivalis Fe-SOD Mn-SOD P. g. SOD P. g. SOD ELISA ELISA SOD SOD SOD microtiter plate ELISA P. gingivalis ATCC 33277 SOD ELISA SOD

pmolnmol P. g. SOD ELISA SOD SOD SOD SOD SOD ELISA SOD P. g. SOD ELISA P. g. SOD ELISA ELISA P. g. SOD pmolnmol P. g. SOD ELISA ELAISA P. gingivalis SOD SOD SOD ELISA SOD P. gingivalis P. g. SOD ELISA SOD P. gingivalis SOD P. gingivalis P. g. SOD ELISA SOD

Cambialistic SOD Fe Mn SOD P. g. SOD P. gingivalis P. gingivalis SOD

Location of main occluding areas and masticatory ability in patients with reduced occlusal support Eichner A1 Eichner B1 B4 C1 C3 mm PM M Eichner

Eichner A1 Eichner B C Eichner C1 Eichner Chi square test ; p Eichner A1 B1 C3 Eichner one-factor ANOVA ; p PM M Student t-test ; p Eichner