LC LC ACQUITY UPLC H-Class ACQUITY UPLC CSH C mm 1.7 µm : THF/ 2/8 A 1 mm / 4/6 THF 2 mg/ml THF/ 2 THF 2 ACQUITY UPLC CSH C 18

ACQUITY UPLC H-Class/QDa Nihon Waters K.K., Tokyo, Japan TAG 1 3 1 1 8 9 TAG TAG TAG TAG TAG UV UV APCI ESI MS TAG Sn-2 Sn-3 Sn-1 C18: ACQUITY UPLC CSH C 18 ACQUITY UPLC H-Class 1. ACQUITY QDa TAG ACQUITY UPLC CSH ACQUITY UPLC H-Class/QDa 1

LC LC ACQUITY UPLC H-Class ACQUITY UPLC CSH C 18 2.1 15 mm 1.7 µm 1865298 55 2 : THF/ 2/8 A 1 mm / 4/6 THF 2 mg/ml THF/ 2 THF 2 ACQUITY UPLC CSH C 18 TAG Partition Number PN 1 TAG PN PN -2 2 PN B 1 mm / 9/1.4 ml / live 1 4 48 7.74 1: Scan ES 1.83e7.5 µl 25 µl A B. 35 65 6 13.5 8 2 6 14.5 1 11 16. 35 65 11 2 4. live 1 44 6.48 46 7.11 Time 5. 6. 7. 8. 9. 1. 11. C16: 1 PN =(161818)-2 2 =48 92.9 5 8.45 52 9.16 1: Scan ES 1.72e7 PN=(181818) -2 3 = 48 93.8 MS MS ACQUITY QDa ESI Scan 1.5 kv 1 V Low 45 V High 5 12 L/hr = 2-125 876.8 877.9 94.8 878.8 97.8 7 75 8 85 9 95 1 15 2. MS MS ESI ESI NH 4 APCI M Scan 1 V 45 V Scan 1 TAG 3 2 Scan TAG 2

プリカーサーイオン (C57H166NH 4 ) Sn-2 H 3 C CH2 フラグメント (C4H754) NH 4 Sn-3 Sn-1 C18: フラグメント (C4H734) CH2 H2C フラグメンント (C4H754) 3. 4 PN 4 PN 5 3 TAG 5 7.78 Tallow 119_34 48 7.74 1 46 7.11 44 5 8.45 6.48 3. 4. 5. 6. 7. 8. 119_37 42 1 5.83 6.48 7.14 7.86 8.5 3. 4. 5. 6. 7. 8. 119_4 7.14 1 7.78 6.53 5.91 7.88 8.51 38 4 5.31 3. 4. 5. 6. 7. 8. 119_42 1 7.76 8.5 6.28 7.1 7.11 8.15 3. 4. 5. 6. 7. 8. オリーブ油 52 9.16 9. 1. 11. 12. 13. ゴマ油 9.2 9. 1. 11. 12. 13. キャノーラ油 9.21 9. 1. 11. 12. 13. 9.2 54 8.71 9.86 牛脂 9. 1. 11. 12. 13. 1: Scan ES 1.83e7 1: Scan ES 1.36e7 1: Scan ES 1.37e7 1: Scan ES 1.5e7 Time 4. 1 1 61.6 599.5-2: Scan ES 4.43e6 コーン電圧 -C18:2 575.5 62.6 45 V -C16: 576.6 881.8 898.893.8 -C18:2 55 6 65 7 75 8 85 9 95 1 1: Scan ES 898.8 6.55e6 C18:2 NH4 C16: C18:2 C18:2 NH4 899.8 コーン電圧 C18:2 1 V 872.8 873.8 9.8 55 6 65 7 75 8 85 9 95 1 5. 3

TAG PN PN MW Adduct 44 16:2 18:1 18:1 C55H986 854.7 NH 4 872.8 63.5 573.5 573.5 44 16: 18:2 18:2 C55H986 854.7 NH 4 872.8 599.5 575.5 575.5 44 16: 18:1 18:3 C55H986 854.7 NH 4 872.8 599.5 577.5 573.5 44 18:1 18:1 18:3 C57H16 88.8 NH 4 898.8 63.5 599.5 599.5 44 18:1 18:2 18:2 C57H16 88.8 NH 4 898.8 61.5 61.5 599.5 46 16: 18:1 18:2 C55H16 856.8 NH 4 874.8 61.5 577.5 575.5 46 18:1 18:1 18:2 C57H126 882.8 NH 4 9.8 61.5 61.5 63.5 48 16: 18:1 18:1 C55H126 858.8 NH 4 876.8 63.5 577.5 577.5 48 18:1 18:1 18:1 C57H146 884.8 NH 4 92.8 63.5 63.5 63.5 5 16: 18: 18:1 C55H146 86.8 NH 4 878.8 65.6 579.5 577.5 5 18: 18:1 18:1 C57H166 886.8 NH 4 94.8 65.6 65.6 63.5 52 18: 18: 18:1 C57H186 888.8 NH 4 96.8 67.6 65.6 65.6 52 18:1 18:1 2: C59H116 914.8 NH 4 932.9 633.6 633.6 63.5 PN MW Adduct 42 18:2 18:2 18:2 C57H986 878.7 NH 4 896.8 599.5 599.5 599.5 44 16: 18:2 18:2 C55H986 854.7 NH 4 872.8 599.5 575.5 575.5 44 18:1 18:2 18:2 C57H16 88.8 NH 4 898.8 61.5 61.5 599.5 46 16: 18:1 18:2 C55H16 856.8 NH 4 874.8 61.5 577.5 575.5 46 16:1 18:1 18:1 C55H16 856.8 NH 4 874.8 63.5 575.5 575.5 46 18:1 18:1 18:2 C57H126 882.8 NH 4 9.8 63.5 61.5 61.5 48 16: 18:1 18:1 C55H126 858.8 NH 4 876.8 63.5 577.5 577.5 48 18:1 18:1 18:1 C57H146 884.8 NH 4 92.8 63.5 63.5 63.5 5 16: 18: 18:1 C55H146 86.8 NH 4 878.8 65.6 579.5 577.5 5 18: 18:1 18:1 C57H166 886.8 NH 4 94.8 65.6 65.6 63.5 52 18: 18: 18:1 C57H186 888.8 NH 4 96.8 67.6 65.6 65.6 52 18:1 18:1 2: C59H116 914.8 NH 4 932.9 633.6 633.6 63.5 PN MW Adduct 38 18:2 18:3 18:3 C57H946 874.7 NH 4 892.7 595.5 597.5 597.5 4 18:1 18:3 18:3 C57H966 876.7 NH 4 894.8 599.5 599.5 595.5 4 18:2 18:2 18:3 C57H966 876.7 NH 4 894.8 599.5 597.5 597.5 42 18:1 18:2 18:3 C57H986 878.7 NH 4 896.8 61.5 599.5 597.5 44 16:1 18:1 18:2 C55H986 854.7 NH 4 872.8 61.5 575.5 573.5 44 18:1 18:1 18:3 C57H16 88.8 NH 4 898.8 63.5 599.5 599.5 46 16:1 18:1 18:1 C55H16 856.8 NH 4 874.8 63.5 575.5 575.5 46 18:1 18:1 18:2 C57H126 882.8 NH 4 9.8 61.5 61.5 63.5 48 16: 18:1 18:1 C55H126 858.8 NH 4 876.8 63.5 577.5 577.5 48 18:1 18:1 18:1 C57H146 884.8 NH 4 92.8 63.5 63.5 63.5 5 16:1 18: 18: C55H146 86.8 NH 4 878.8 67.6 577.5 577.5 5 18: 18:1 18:1 C57H166 886.8 NH 4 94.8 65.6 65.6 63.5 5 18:1 18:1 2:1 C59H186 912.8 NH 4 93.8 631.6 631.6 63.5 52 18: 18: 18:1 C57H186 888.8 NH 4 96.8 67.6 65.6 65.6 52 18:1 18:1 2: C59H116 914.8 NH 4 932.9 633.6 633.6 63.5 54 18:1 18:1 22: C61H1146 942.9 NH 4 96.9 661.6 661.6 63.5 PN MW Adduct 44 12: 16: 18:1 C49H926 776.7 NH 4 794.7 577.5 521.5 495.4 44 14: 14: 18:1 C49H926 776.7 NH 4 794.7 549.5 549.5 495.4 44 18:1 14:1 16: C51H946 82.7 NH 4 82.7 577.5 547.5 521.5 44 14:1 18:1 18:1 C53H966 828.7 NH 4 846.8 63.5 547.5 547.5 46 14: 16: 16: C49H946 778.7 NH 4 796.7 551.5 523.5 523.5 46 14: 16: 18:1 C51H966 84.7 NH 4 822.8 577.5 549.5 523.5 46 16: 16:1 18:1 C53H986 83.7 NH 4 848.8 577.5 575.5 549.5 46 16: 16:1 2:2 C55H16 856.8 NH 4 874.8 63.5 61.5 549.5 46 16: 18:1 18:2 C55H16 856.8 NH 4 874.8 61.5 577.5 575.5 48 16: 16: 18:1 C53H16 832.8 NH 4 85.8 577.5 577.5 551.5 48 16: 18:1 18:1 C55H126 858.8 NH 4 876.8 63.5 577.5 577.5 48 18:1 18:1 18:1 C57H146 884.8 NH 4 92.8 63.5 63.5 63.5 5 16: 16: 18: C53H16 834.8 NH 4 852.8 579.5 579.5 551.5 5 16: 18: 18:1 C55H146 86.8 NH 4 878.8 65.6 579.5 577.5 5 18: 18:1 18:1 C57H166 886.8 NH 4 94.8 65.6 65.6 63.5 52 16: 18: 18: C55H166 862.8 NH 4 88.8 67.6 579.5 579.5 52 18: 18: 18:1 C57H186 888.8 NH 4 96.8 67.6 65.6 65.6 54 18: 18: 18: C57H116 89.8 NH 4 98.9 67.6 67.6 67.6 4

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