Agilent SPE SPE
...3...4...5...6...7...8 OPT...10 SCX...12 SAX...14 DVB...16 PS-DVB...17...18 C18EC...18 C18 ODS...19 C8...20 C2...21...21...22 (CN)...23...23...24 Si-SCX...25 Si-SAX...26...27 Florisil PR...27 A...28 B...29 N...29...30 C8/Si-SCX...31 SPE...32 Evidex...32...33...34 QuEChERS...35...36...36...37 96...38 2
SPE (SPE) HPLC SPE SPE Agilent SPE HPLC ISO-9001 SPE 96 3 www.agilent.com/chem/jp SPE 3
Agilent SPE < 2000 ( ) MeOH ( ) ACN () Et OAc ( ) THF ( ) CH 2 Cl 2 () Et 2 O () C5 (n-) C6 (n-) ic8 () CEX AEX n-bpc LSC RPC n-bpc LSC RPC SCX SAX OPT DVB PS-DVB OPT DVB PS-DVB Si-SCX C8/Si-SCX ( ) Si-SAX (WAX) C18 C18EC C8 C2 C18 C18EC C8 C2 Florisil PR A B N Florisil PR A B N EVIDEX 4
Agilent Bond-Elut Plexa Oasis HLB Strata-X Bond-Elut ENV Strata SDB-L Supelclean ENVI-Chrom P Bond-Elut Plexa PCX Oasis MCX Strata X-C Oasis MAX Bond-Elut C18 Discovery DSC-C18 Sep-Pak tc18 Strata C18-E Supelclean ENVI-18 Supelclean LC-18 Bond-Elut C18OH Sep-Pak C18 Strata C18-U InsertSep C18 Bond-Elut C8 Sep-Pak C8 Strata C8 InsertSep C8 Bond-Elut C2 Sep-Pak tc2 InsertSep C2 Discovery DSC-Ph Strata Phenyl (PH) Supelclean LC-Ph InsertSep PH Bond-Elut Certify-I Strata Screen-C () Bond-Elut SI Discovery DSC-Si Sep-Pak Silica Strata Si-1 Bond-Elut SI Discovery DSC-Si Sep-Pak Silica Strata Si-1 InsertSep SI Bond-Elut FL Sep-Pak Florisil Strata FL-PR Supelclean LC Florisil Supelclean ENVI Florisil InsertSep FL Bond-Elut NH2 Discovery DSC-NH2 Sep-Pak Amino Propyl Strata NH2 Supelclean LC-NH2 InsertSep NH2 Bond-Elut 2OH Discovery DSC-Diol Supelclean LC-Diol Bond-Elut CN-U Discovery DSC-CN Sep-Pak Cyano Propyl Strata CN Supelclean LC-CN InsertSep CN Bond-Elut AL-A,B,N Sep-Pak Alumina A, B, N Supelclean LC-Alumina A,B,N InsertSep AL A, B, N Bond-Elut SAX Discovery DSC-SAX LC-SAX Sep-Pak AccellPlus QMA Strata SAX InsertSep SAX Bond-Elut SCX Discovery DSC-SCX Sep-Pak AccellPlus SCX Strata SCX Supelclean LC-SCX InsertSep SCX Supelclean ENVI-Carb InsertSep GC Agilent OPT PS-DVB SCX SAX C18EC C18 C8 C2 Evidex Florisil PR A B N Si-SAX Si-SCX 5
Agilent HPLC LC/MS GC ( ) ( ) ( ) / GC 6
Agilent SPE Agilent SPE Agilent SPE ph 0 14 ph 7
A/N/B Log P < 1.5 / pka < 3 SAX Log P > 1.3 pka > 3 pka < 10 OPT Log P < 1.5 / pka > 10 SCX 0.1% 2% 1 ml/min (ph 7) 1 2 50 mm (ph 7) 5% 5 10% 2% 2 1 1 1 2% 0.1% 3% NH 4 OH 8
1 () SCX OPT SAX SCX (A) (N) (B) * 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 3 ml 2 ml 2 ml 2 ml 2 ml 2 ml 6 ml 3 ml 3 ml 5 ml 4 ml 4 ml 96 200 µl 200 µl 200 500 µl 200 µl 200 500 µl www.agilent.com/chem/jp * 9
Agilent OPT SPE Agilent Optimized Polymer Technology (OPT) OPT ( ) ph 0 14 OPT GC LC OPT ph pka 0.1% OPT OPT R N R O R 30 mg 1 ml 100 5982-3013 60 mg 3 ml 50 5982-3036 150 mg 6 ml 30 5982-3067 96 10 mg 5982-3096 10
OPT 140 120 100 (%) 80 60 40 20 0 OPT ( RSD) OPT ( 5989-8869EN) 11
Agilent SCX (SCX) SCX (log P) ph 0 14 SCX R 1 R 2 SO 3-30 mg 1 ml 100 5982-3213 60 mg 3 ml 50 5982-3236 150 mg 6 ml 30 5982-3267 SCX 25 12
3% (%) 120 100 80 60 40 20 0 105 105 107 94 91 94 92 97 94 88 () 2 () (3% ) ph SAX ( 5989-8945EN) 8 13
Agilent SAX Agilent (SAX) 3 SAX (log P) SAX ph 0 14 SAX N R 3 R 1 R 2 30 mg 1 ml 100 5982-3313 60 mg 3 ml 50 5982-3336 150 mg 6 ml 30 5982-3367 SAX 26 14
2 SAX SAX (%) 120 100 80 60 40 20 0 2% (2% ) SAX 100 98+/- 0.7 93+/- 0.6 93+/- 1.3 97+/- 1.0 98+/1 1.0 98+/- 1.3 (%) 80 60 40 20 0 1 2 ph SAX ( 5989-8944EN) 8 15
Agilent DVB DVB SPE C18 PS-DVB 100% DVB SPE ph 0 14 SPE DVB 30 mg 1 ml 100 5982-3113 60 mg 3 ml 50 5982-3136 150 mg 6 ml 30 5982-3167 16
Agilent PS-DVB PS-DVB (PS-DVB) (600 m2/g) C18 C8 (75 160 µm) PS-DVB [-CH - CH 2 - CH - CH 2 - CH - CH 2 -] n 500 mg 6 ml 30 5982-3465 1000 mg 6 ml 30 5982-3460 [-CH - CH 2 C - CH 2 - CH - CH 2 -] n - 17
Agilent SPE SPE 45 µm 60 Å SPE 3 Agilent () SPE () C18EC Si O Si (CH 2 ) 17 CH 3 O Si O Si(CH 3 ) 3 C18 C18EC (ODS) (EC) C18 C18EC 25% C18EC 100 mg 1 ml 100 5982-1311 200 mg 3 ml 50 5982-1332 500 mg 3 ml 50 5982-1335 1000 mg 6 ml 30 5982-1360 500 mg 6 ml 30 5982-1365 25 g 5982-1382 18
C18 C18 ODS ( ) ( ) SPE C18 24% Si O Si (CH 2 ) 17 CH 3 O Si OH (C18) C18 100 mg 1 ml 100 5982-1111 200 mg 3 ml 50 5982-1132 500 mg 3 ml 50 5982-1135 500 mg 6 ml 30 5982-1165 1000 mg 6 ml 30 5982-1160 25 g 5982-1182 19
Si O Si (CH 2 ) 7 CH 3 O Si OH C8 C8 ( ) () SPE SPE (C8) C8 100 mg 1 ml 100 5982-1011 200 mg 3 ml 50 5982-1032 500 mg 3 ml 50 5982-1035 500 mg 6 ml 30 5982-1065 25 g 5982-1082 20
C2 C2 SPE () 3 C8 C18 O Si Si O OH Si CH 2 CH 3 C2 100 mg 1 ml 100 5982-1411 200 mg 3 ml 50 5982-1432 500 mg 3 ml 50 5982-1435 500 mg 6 ml 30 5982-1465 1000 mg 6 ml 30 5982-1460 SPE () (pi-pi) O Si Si O OH Si 100 mg 1 ml 100 5982-1511 200 mg 3 ml 50 5982-1532 500 mg 3 ml 50 5982-1535 25 g 5982-1582 21
Agilent () SPE () O Si Si OH OH SPE () 1 () ph 100 mg 1 ml 100 5982-2211 200 mg 3 ml 50 5982-2232 500 mg 3 ml 50 5982-2235 500 mg 6 ml 30 5982-2265 1000 mg 6 ml 30 5982-2260 25 g 5982-2282 22
(CN) (CN) SPE () Si O Si O OH Si (CH 2 ) 3 CN (CN) 100 mg 1 ml 100 5982-1711 500 mg 6 ml 30 5982-1765 1000 mg 6 ml 30 5982-1760 SPE () Si O Si O OH Si (CH 2 ) 3 OCH 2 CH CH 2 OH OH 500 mg 3 ml 50 5982-1635 23
(NH 2 ) Si O Si (CH 2 ) 3 NH 2 O Si OH (NH 2 ) (NH 2 ) SPE () 2 -OH -NH -SH ph 7.8 (NH 2 ) 100 mg 1 ml 100 5982-1811 200 mg 3 ml 50 5982-1832 500 mg 3 ml 50 5982-1835 500 mg 6 ml 30 5982-1865 1000 mg 6 ml 30 5982-1860 25 g 5982-1882 24
Agilent ph (Si-SCX) Si-SCX SPE () 0.3 meq/g Si O Si O Si OH (CH 2 ) 2 SO 2 - H + (Si-SCX) SCX 100 mg 1 ml 100 5982-2111 200 mg 3 ml 50 5982-2132 500 mg 3 ml 50 5982-2135 500 mg 6 ml 30 5982-2165 1000 mg 6 ml 30 5982-2160 25
Si O Si (CH N + (CH 3 ) 3 CI 2 ) 3 O Si OH SAX (Si-SAX) Si-SAX SPE () pka ph 0.6 meq/g (Si-SAX) 100 mg 1 ml 100 5982-2011 200 mg 3 ml 50 5982-2032 500 mg 3 ml 50 5982-2035 500 mg 6 ml 30 5982-2065 1000 mg 6 ml 30 5982-2060 25 g 5982-2082 26
Agilent SPE SPE / Florisil PR Florisil PR Florisil PR 200 mg 3 ml 50 5982-4332 500 mg 3 ml 50 5982-4335 500 mg 6 ml 30 5982-4365 1000 mg 6 ml 30 5982-4360 25 g 5982-4382 27
A () A 50 200 µm A 500 mg 3 ml 50 5982-4035 1000 mg 6 ml 30 5982-4060 25 g 5982-4082 28
B () B 50 200 µm B 500 mg 3 ml 50 5982-4135 1000 mg 6 ml 30 5982-4160 25 g 5982-4182 N () N 50 200 µm (N O P S ) N 500 mg 3 ml 50 5982-4235 1000 mg 6 ml 30 5982-4260 25 g 5982-4282 29
100 mg 1 ml 100 5982-4411 250 mg 3 ml 50 5982-4432 500 mg 6 ml 30 5982-4465 25 g 5982-4482 30
C8/Si-SCX SPE SPE (C8) (Si- SCX) C8 Si-SCX UV LC/MS 3 C8/Si-SCX 100 mg 1 ml 100 5982-1911 200 mg 3 ml 50 5982-1932 500 mg 3 ml 50 5982-1935 500 mg 6 ml 30 5982-1965 1000 mg 6 ml 30 5982-1960 31
Agilent SPE Evidex SPE Evidex SPE SAMHSA ( ) / PCP () () / THC-COOH ( ) (RSD 5% ) Evidex SPE 200 mg 3 ml 50 5982-2332 400 mg 6 ml 30 5982-2364 GC EDDP DB-5ms EVDX (5% )- GC (mm) (m) (µm) ( ) DB-5ms EVDX 0.20 25 0.33-60 325/350 128-8522 32
SPE Agilent SPE 35 QuEChERS C18EC 25 g 5982-1382 C18 25 g 5982-1182 C8 25 g 5982-1082 25 g 5982-4482 25 g 5982-1582 25 g 5982-2282 (NH 2 ) 25 g 5982-1882 Si-SAX 25 g 5982-2082 A () 25 g 5982-4082 B () 25 g 5982-4182 N () 25 g 5982-4282 Florisil PR 25 g 5982-4382 33
SPE Agilent SPE 1 ml 50 5982-9301 SPE 3 ml 50 5982-9302 SPE 6 ml 50 5982-9303 SPE 35 ml 50 5982-9304 SPE 70 ml 50 5982-9305 SPE 1 ml 100 5982-9306 SPE 3 ml 100 5982-9307 SPE 6 ml 100 5982-9308 SPE 35 ml 100 5982-9309 SPE 75 ml 100 5982-9310 34
SPE QuEChERS QuEChERS Steven J. Lehotay CVUA Michelangelo Anastassiades QuEChERS "Quick, Easy, Cheap, Effective, Rugged and Safe" www.quechers.com QuEChERS 100 g 5982-8082 PSA ( 1 2 ) 25 g 5982-8382 25 g 5982-4482 C18 25 g 5982-1182 Si-SAX 25 g 5982-2082 www.agilent.com/chem/jp 35
12 16 x 100 mm 20 16 x 100 mm 5982-9110 5982-9120 20 5982-9120 5982-9100 Luer 5982-9104 5982-9105 5982-9103 36
/ 5982-9106 5982-9107 5982-9108 12 12 5982-9111 12 5982-9112 12 5982-9113 13 x 75 mm 12 5982-9114 13 x 100 mm 12 5982-9115 16 x 75 mm 12 5982-9116 16 x 100 mm 12 5982-9117 20 20 5982-9121 20 5982-9122 20 5982-9123 13 x 75 mm 20 5982-9124 13 x 100 mm 20 5982-9125 16 x 75 mm 20 5982-9126 16 x 100 mm 20 5982-9127 12 5982-9110 37
12 20 20 5982-9100 20 5982-9101 20 5982-9102 20 5982-9103 25 5982-9104 5982-9105 12 5982-9109 96 96 96 5185-5776 96 96 5185-5776 1. - 5185-5797 2. 5185-5786 3. 5185-5785 4. 5185-5783 5. 5185-5784 6. 5185-5798 7. 96 38
96 96 50 5042-1389 96 0.5 ml 120 5042-1385 96 0.5 ml 10 5042-1386 96 5065-4402 0.35 ml 96 O- 5185-5779 /96 Agilent 1 ml 12 mm 5185-5787 5185-5775 5185-5781 Agilent 0.5 ml 29 mm 5185-5780 2 mm 96 25 5185-5782 96 5185-5798 96 5185-5799 96 5185-5778 96 Luer 25 5185-5789 96 5185-5783 96 5185-5785 8 Luer 50 5185-5788 96 5185-5786 96 () 5185-5784 / 5185-5790 96 5042-1389 O- 5185-5779 39
Agilent SPE GC/LC Agilent J&W GC Über One GC Agilent ZORBAX Eclipse Plus LC 40 (LOD) (LOQ) www.agilent.com/chem/jp 0120-477-111 Agilent Technologies, Inc. 2008 Printed in Japan August 7, 2008 5989-9334JAJP