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21 1) -1 2) -2 10 m 5 7 23 6 1)2) 3) -1-2 H23.4.25

-15-20 4 75 5 1) -1 (5) (3) (3) (2) (1) (1) (1) (1) (1) (1) (1) (1) (1) (1) (1) (1) 1) ( 23 6 8 ) 2) 22 3) 23 6 17 0617 3 4) 23 7 11 0711 1

2007 2010 11 2011 4 2012 7 18 30 15 13 2 2 HA N RT-PCR BLAST B19 18 30 1 1 D9 17 29 1 1 E 3 5 B19 D9 2011 6 E B19 2011 4 7 WHO 17 4 23.5

PCR 56 AG 7 ELISA 1740/41 (Adenovirus not typed)elisa 2012 11 PCR 2012 4 2013 2 52 PCR ( 2 ) 2 1 C4 PCR ( 1 1)DNA QIAamp Viral RNA Mini Kit(QIAGEN) DNA template BLAST PCR526 2ELISA PCR 5

PCR PCR (2 ) ELISA 40/41 PCR 41 ELISA 40/41 2 PCR PCR 110 copy/reaction ELISA PCR ELISA PCR 1 PCR 5 6 2 5 7 11 12 40/41 41 PCR

NV AstVNV AiV 40/41 AstV RNA 1 8 8 1975 AiV RNA ABC 1989 NV AstVAiV RT-PCR 23 4 25 2 NV 32 AstVAiV cdna PCR PCR 1 PCR (bp) AstV AC1AC230 230 AiV 1st CC 519 2nd C94246 224 PCR AstV DW 10ExTaq buffer(takara) 2.5mM dntp Primer AC25M Primer AC23025M ExTaq (Takara) cdna Total 94 3min 94 30sec 50 15sec 72 30sec 72 5min 4 40 AiV 1st PCR DW 10ExTaq buffer(takara) 2.5mM dntp Primer C10M Primer C10M ExTaq (Takara) cdna Total 2nd PCR DW 10ExTaq buffer(takara) 2.5mM dntp Primer C94b10M Primer 246k10M ExTaq (Takara) 1stPCR 10 Total 18.48l 2.5l 2.0l 0.2l 0.2l 0.125l 1.5l 25.0l 34.75l 5.0l 4.0l 0.5l 0.5l 0.25l 5.0l 50.0l 34.75l 5.0l 4.0l 0.5l 0.5l 0.25l 5.0l 50.0l

(1st2nd ) 95 2min 94 20sec 55 30sec 40 72 1min 72 7min 4 AstVAiV2 3 AstV 32 2 230bp 2 BLAST AstV AstV 1 0 0 9 AiV 30 2 AstV AiV AstV AiV 32 301 2 0 6.3 0 2 2.4 0 1AstV 2 23 4 25 2 84 3 AstV 12-84 24 2 1 10 12-109 24 2 8 09 M 1 2 3 4 5 6 7 8 9 M AstV PCR 10 NV 80 3 11 4 AstV 2.4 2 AstV RT-PCR AstV AiV 19871998 12 37 12 32.4 12 11 10 NV NVAstV 0.26.4 AiV AiV NV AiV 500bp MDNA MakerLane1:12-84Lane2:12-109 Lane8:Negative controllane9positive control 2 1 22 2,138-145, 3,,12,29-32

(HCHO) HCHO 1) HCHO 1. 5 ( 3 2 ) 2. (1) HCHO () HCHO 400 ppm4 ppm (2) () HCHO HCHO (400 ppm) 20 ml 0.3 ml 2.0 g 4 ppm (3) 15 g 0.3 ml 0.2 ml 100 ml (4) 5 g 25 ml 3. UV-2450 4. (1) 2 g 3 ml 180 ml 200 ml ( 1.) 2 g 50 ml 3 ml 20 ml 200 ml 1. (2) 0.2 g 5 ml 0.3 ml 9030 2.0 g 3 (2,000 rpm10min) 20 ml ( 2.) 0.2 g 5 ml 0.3 ml 9030 2.0 g 3 (2,000 rpm) 10min 20 ml 2.

5. HCHO () HCHO 5 ml 5 ml 4030 5 ml 5 ml 5 ml 5 ml HCHO 415 nm 1. (1) 5 ( 1.) 1. 2) A 75 μg/g B 75 μg/g C 75 μg/g A 75 μg/g B 75 μg/g (2) 2 g 800 μg N = 3 ( 2.) 2. HCHO (n = 3) A 79.8 70.9 B 94.8 75.9 C 96.3 72.4 A 86.7 73.8 B 88.7 83.7 2. (1) 0.2 g 80 μg N = 3 ( 2.)HCHO HCHO (4 ppm) HCHO (4 ppm) 2 HCHO 5 70 100% HCHO () HCHO 1) Masaki S, et al, 59, 2010 2) ( 49 34 ) *%

1 19 11 15 115001 22 12 24 25 12 13 2 (1)HPLC 1) (2) 2) OTC OTC Agilent HPLC1260 LC-10

OTC 5- OTC 2) ( )

23 27 39 DRC 40 DRC 45 46 51 52 DRC 55 56 DRC 59 60 63 66 75 80 DRC 85 98 111 121 133 138 139 140 152 178 181 184 208 232

Na Al Ca Ti V Cr Fe Co Ni Cu Zn As Rb Cd Sb 23 27 39 DRC 40 DRC 45 46 51 52 DRC 55 56 DRC 59 60 63 66 75 80 DRC 85 98 111 121 133 137 139 140 152 178 181 186 208 232

39 10 1 500km 50 70 (db) 75dB 60 75 (75 ) 18 75 24 75 5 20 24 22 5 NL-06(RION ) A Slow 25m 20 1 V km/h l mt s 20 LA,Smax 10 LA,Smax nn = 10 LA,Smax,i i db 20 24 70dB

20 24 70dB 20 24 20 24 300 19 21 700 N700 20 300 700 N700 300 700 N700 700 N700 20 24 300 700 N700 700 N700 270 /h http://www.env.go.jp/ 22 5 300 700

24 1.2km 2 2m COD 13 18 6 1 5 COD 19 23 1 3 ) 3 2 19 4 23 3 1 3 COD( TN( TP)( NO 3 -N)( NO 2 -N) ( NH 4 -N) ( PO 4 -P) 8 1 20 a 1 3 CODTNTP 2-12-3 COD 5.0mg/L TN 3 TP 1920

COD 2-2 TN NO 3 -NNO 2 -NNH 4 -N 3-13-2 NO 3 -NNO 2 -NNH 4 -N 3 TN 3040 CODTNTP a NO3-N 4-14-6 COD 11 1 TN TP a 11 1

a COD NO 3 -N COD NO3-N 2 3 CODTNTP 5-15-3 COD TN 2 2 3 TN

80 NO 3 -N TP 2 COD TP TN 1 CODTN TP 2 NO3-N 3 COD TP 4 TN 2

49 (

9

- - -

1)

(NP)C 15 H 24 O 200 NP NP200517,000t 20096,000t20050.8t20093.6t NP22812 24 371 24822248 127NP 0.0006~0.002mg/L0.0007~0.001mg/L GC/MS 25121321 14BZnNP NPNPGC/MS NP Agilent GC(Agilent6890) MS(JMS-GCmate)GC DB-5MS(0.25mm 0.25 m 30m) NP 4- PCB 5000 () () NP 10mg 100mL 100 NP (1g/mL)

24 8 127 NP GC/MS 1 ( TIC) 1() NP 2 13 NP 13 2 NP5 NP6 NP9 NP10 SIM 13 NP NP12 TIC GC 8/min 8/min 4/min 1.5/min NP ()NP9 NP10 NP5 NP6 2 400V NP12 TIC ( 191) 4/min 1.5/min 1 NPTIC 400V 500.2V NP12 NP12 2 TIC191IC

( IC)( 2 ) 500.2V 191 IC NP12 TIC NP 13 NP5 NP6 TIC NP GC/MS NP HP http://www.env.go.jp/

1 23 9 24 6 24 2 23 8 25 2 1 4 ( )(3000rpm,10min NDR-2000 himac CR 20G 23 24 25 24 2 23 24 25 1 12.0 31.834.5 7380

57.7

23 3 1 23 NaI (-1) ( GC2020) NaI 1cm50cm 100cm (-2) NaI ( TCS-172B) -1-2 -1-1

(I-131)(Cs-134Cs-137) (I-131)(Cs-134 Cs-137) 0.03 0.08Sv/h -2-2 14Bq/kg 15Bq/kg 100Bq/kg (I-131)(Cs-134Cs-137) -3 0.07 0.13Sv/h 0.23Sv/h -3 (Cs-134) 27Bq/kg(Cs-137) 45Bq/kg 72Bq/kg 8,000Bq/kg (Cs-134Cs-137)