MALDI-TOF-MS Cooking Book Ver. 1.0 Prepared by T.Nirasawa 2-May-03
Table matrix mono MW for size (g) Parts No. (Daltonics) -cyano-4-hydroxy-cinnamicacid 189.17 # peptide (CCA) sinapinic acid (SA) 224.22 2,5-dihydroxy-benzoic acid (2,5-DHB) Super DHB (SDHB) 3-Hydroxypicolinic acid (3-HPA) α-cyano-4-hydroxycinnamic acid methyl ester (CNME) 2,3,4 -Trihydroxyacetophenone (2,3,4-THAP) 2,4,6 Trihydroxyacetophenone (2,4,6-THAP) 154.12 187.20 # peptide # protein # protein # glycopeptide # glycoprotein # oligosaccharide # polymer # glycopeptide # glycoprotein # oligosaccharid 139.11 203.20 168.15 168.15 6-aza-2-thiothymine (ATT) 143.02 trans-3-indole-acrylic acid (IAA) Dithranol (DIT) 187.20 226.23 #201345 #203073 #201346 #203074 #209813 # polymer # aromatic compound # polymer # aromatic compound
CCA in TA ( ) 4l (5pmol/l) 1l mix 1l on target #26755 MTP 384 massive target T #26993 MTP 384 massive target gold plated T #209519 MTP 384 target ground steel T F #209520 MTP 384 target plate polished steel T F
Double Layer Preparation CCA in acetone ( ) CCA in TA ( ) 4l (5pmol/l) 1l l mix 1l #209520 MTP 384 target plate polished steel T F
SA in TA ( ) 4l (10pmol/l) 1l mix 1l on target #26755 MTP 384 massive target T #26993 MTP 384 massive target gold plated T #209519 MTP 384 target ground steel T F #209520 MTP 384 target plate polished steel T F
Double Layer Preparation SA in EtOH ( ) SA in TA ( ) 4l (10pmol/l) 1l l mix 1l #209520 MTP 384 target plate polished steel T F
DHB in TA (10mg/ml) 4l (5pmol/l) 1l mix 1l on target #26755 MTP 384 massive target T #26993 MTP 384 massive target gold plated T #209519 MTP 384 target ground steel T F
DHB in TA (10mg/ml) 4l (10pmol/l) 1l mix 1l on target #26755 MTP 384 massive target T #26993 MTP 384 massive target gold plated T #209519 MTP 384 target ground steel T F
SDHB in TA (10mg/ml) 4l (10pmol/l) 1l mix 1l on target #26755 MTP 384 massive target T #26993 MTP 384 massive target gold plated T #209519 MTP 384 target ground steel T F
2,4,6-THAP in 50ACN (10mg/ml) 4l (10pmol/l) 1l mix 1l on target #26755 MTP 384 massive target T #26993 MTP 384 massive target gold plated T #209519 MTP 384 target ground steel T F #209520 MTP 384 target plate polished steel T F
DHB in 30%EtOH (10mg/ml) 4l (5pmol/l) 1l mix 1l on target #26755 MTP 384 massive target T #26993 MTP 384 massive target gold plated T #209519 MTP 384 target ground steel T F
AnchorChip STD ZipTip CCA stock 1mg/ml acetone CCA working( M ) 2:1= EtOH : acetone ( W ) 0.1%TFA in H 2 O ( R ) 6:3:1 EtOH : acetone : 0.1%TFA 0.53l 600m anchor Matrix ( M ) 1l 0.53l 600m anchor Matrix ( M ) 1l W 4l R 1l #209513 MTP AnchorChip 600/384 T F
Thin Layer Affinity AnchorChip Preparation CCA ( M ) 2l 600m anchor 1 2 CCA stock 97% acetone : 0.1% TFA CCA working( M ) 1:1 CCA stock : 97% acetone + 0.1% TFA ( W ) 0.1%TFA in H 2 O ( R ) 6:3:1 EtOH : acetone : 0.1%TFA 14l 600m anchor 3 W 35l W 35l anchor Thin Layer PSD,LIFT R 1l #209513 MTP AnchorChip 600/384 T F
DNA RNA oligo 3HPA(10 mg/ml)+ Diammonium hydrogen citrate (DAC 1 mg/ml) in H2O DNA(RNA) pmol/l 3HPA 1l 400m anchor NH4+ 1l 00m anchor MTP AnchorChip 400/384 T F
DNA RNA oligo 2,4,6 Trihydroxyacetophenone (2,4,6-THP) in 50% ACN (10mg/ml) 2,3,4 -Trihydroxyacetophenone (2,3,4-THP) in 50% ACN (10mg/ml) Diammonium hydrogen citrate (e.g. Fluka Part No. 09833) in 50% ACN (DAC 1 mg/ml) THAP 2:1= 2,4,6-THP: 2,3,4-THP+DAC DNA(RNA) pmol/l NH4+ THAP mix 1 #209520 MTP 384 target plate polished steel T F
Double stranded DNA 6-aza-2-thiothymine (ATT) DNA(DS) pmol/l NH4+ ATT 10mg/ml in 50% ACN 1 #209520 MTP 384 target plate polished steel T F
Polymer DHB in TA (10mg/ml) 4l polymer (20pmol/l) 1l mix 1l on target N2 Table 2
Polymer matrix in THF(10mg/ml) 8l polymer (20pmol/l) 2l mix 3l on target Table 2
Table Polymer matrix mono MW for size (g) Parts No. (Daltonics) Salt α-cyano-4-hydroxy-cinnamicacid 1 #201344 189.17 Tween 20 (CCA) 5 #203072 1 #201345 sinapinic acid (SA) 224.22 Polyacrylic acid 5 #203073 2,5-dihydroxy-benzoic acid PEG 1 #201346 NaCl 154.12 (DHB) PPG 5 #203074 KCl 2,4,6 Trihydroxyacetophenone 1 #206154 168.15 Poly(ethylene terephthalate) (2,4,6-THAP) 5 #206153 PMMA PS silver trifluoroacetate trans-3-indole-acrylic acid 1 #209803 187.20 Poly(butyl acrylate) silver nitrate (IAA) 5 #209801 Dendrimer Silver (I) acetylacetonate Polybutadiene Polystyrene PPG Dithranol PMMA 1 #209783 silver trifluoroacetate 226.23 (DIT) Poly(methyl acrylate) 5 #209785 silver nitrate Poly(ethylene terephthalate) Polycarbonate Fluka silver trifluoroacetate all-trans-retinoic acid 300.40 PS ( > 60kDa ) 95152 silver nitrate 9-nitro-anthracene Fullerene Fluka 223.23 (9NA) Dendrimer 72740 5-Chlorosalicylic acid Aldrich 172.57 Si oil (5CSA) C7,090-8 2 -(4-hydroxyphenylazo)benzoicacid 242.24 Polycarbonate Nylon Fluka 54793 (HABA) Tween 85 1,4-Bis(5-phenyloxazol-2-yl)benzene Polyisoprene Fluka 364.40 Silver (I) acetylacetonate (POPOP) Polybutadiene 15150 3-amino-quinoline Fluka 144.18 basic compound (3AQ) 7336 2,6-Dihydroxyacetophenone Fluka 152.15 (DHAP) 37468