&
Novex 7iBlot BenchPro 4100 2
5 11 12 14 16 18 20 21 22 23 3
NuPAGE 5 Bolt & 8 10 11 NuPAGE 5 Bolt 8 10 11 2 New 1.5 200 BT* 30 400 TA* 12 BT* 8 TA* 0.3-2 6 500 6 500 16 1 2 4-25 4-25 2-8 2-8 35 min 35 min 90 min 90 min / 10 25 µl1 mm 37 µl1.5 mm 40 µl1 mm 25 µl1 mm 37 µl1.5 mm 25 µl1 mm 37 µl1.5 mm 210 10 510 : 8 x 8 cm : 8 x 13 cm : 8 x 8.3 cm : 8 x 8 cm : 8 x 13 cm : 8 x 8 cm : 8 x 13 cm 8%, 10%, 12%, 4 12% BT* 7%, 3 8%TA* 8%, 10%, 12%, 4-12% 4%, 6%, 8%, 10%, 12%, 4 20%, 4 12%, 14%, 16%, 18%, 8 16%, 10 20% *BT=TA= 4
NuPAGE NuPAGE NuPAGE ph NuPAGE NuPAGE 2150 NuPAGE ph ANuPAGE B - 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 35 12 NuPAGE - ANuPAGE 4-12 Bis-Tris B4-20 - Lanes1, 6, 7, 10 Mark12 Unstained Protein Standard Lane2 High Protein Load4 proteins-6 g /proteinlane3 Broad Range Standard Lanes 4,5E. coli extract Lane 8 SeeBlueR Pre- Stained Protein StandardLane 9MultiMarkR Multi-colored Protein Standard 2 8%, 10%, 12%, 4 12% 1.5 200 12 35 50 min 7%, 3 8% 30 400 8 60 min 150 NuPAGE NuPAGE 8 x 8cm8 x 13cm XCell SureLock Mini-Cell2 XCell4 SureLock Midi-Cell4 26100 NuPAGE XCell SureLock Mini-Cell NuPAGE XCell4 SureLock Midi-Cell EI0001 =SureLock! 2 WR0100 4 1100 5
NuPAGE NuPAGE 2 MES MOPS 12 MES MOPS 10% 20% 30% 8% 10% 4 12% 12% 3 8% 7% MES MOPS MES MOPS MES MOPS MES MOPS TA TA 2 2 2 2 2 2 500 2 290 500 2 2 290 2 116 40% 97 % of length of gel 50% 116 97 66 60% 55 66 1. NuPAGE & 70% 80% 3.5 55 * NuPAGE Novex Sharp LC5800, Pre-stained; LC5801, Unstained ** NuPAGE HiMark LC5688 90% 100% 3.5 3.5 3.5 Ordering information NuPAGE *8x8cm 1021,500 1.0 mm 1.5 mm 10 wells 12 wells 15 wells 17 wells 2D well IPG well 10 wells 15 wells 2D well 10% NP0301BOX NP0302BOX NP0303BOX NP0306BOX NP0315BOX NP0316BOX NP0317BOX 12% NP0341BOX NP0342BOX NP0343BOX NP0349BOX NP0346BOX 4 12% NP0321BOX NP0322BOX NP0323BOX NP0329BOX NP0326BOX NP0330BOX NP0335BOX NP0336BOX NP0337BOX 7% EA0355BOX EA03552BOX EA03555BOX EA0358BOX EA03585BOX 3 8% EA0375BOX EA03752BOX EA03755BOX EA0376BOX EA0378BOX EA03785BOX * XCell SureLock Mini-Cell5 NuPAGE * 8x8cm 24,600 1.0 mm 1.5 mm 10 wells 12 wells 15 wells 17 wells 10 wells 15 wells 10% NP0301PK2 NP0302PK2 12% NP0341PK2 NP0342PK2 4 12% NP0321PK2 NP0322PK2 NP0323PK2 NP0329PK2 NP0335PK2 NP0336PK2 3 8% EA0375PK2 EA03752PK2 * XCell SureLock Mini-Cell5 NuPAGE *8x13cm 1031,00032,800 Midi Gel Midi Gel ** 12+2 wells 20 wells 26 wells 12+2 wells 20 wells 26 wells 8% WG1001BOX WG1002BOX WG1003BOX WG1001A WG1002A WG1003A 10% WG1201BOX WG1202BOX WG1203BOX WG1201A WG1202A WG1203A 4 12% WG1401BOX WG1402BOX WG1403BOX WG1401A WG1402A WG1403A 3 8% WG1601BOX WG1602BOX WG1603BOX WG1601A WG1602A WG1603A * XCell 4 SureLock Midi-Cell5 ** Midi Gel WA0999 Bio-Rad Criterion Cell 6
NuPAGE XCell SureLock Mini-Cell 1 EI0001 68,400 XCell 1 kit EI9051 60,200 XCell SureLock & Xcell EI0001 +EI9051 1 kit EI0002 127,500 XCell4 SureLock Midi-Cell4 1 WR0100 117,800 PowerEase 500 Power Supply 1 EI8600 288,000 NuPAGE Sample Reducing Agent10X 250 µl NP0004 2,300 10 ml NP0009 8,800 NuPAGE 15 ml NP0005 4,000 NuPAGE LDS 4X 10 ml NP0007 2,000 250 ml NP0008 18,300 NuPAGE MOPS SDS 20X 500 ml NP0001 10,000 5 L NP0001-02 81,200 NuPAGE MES SDS 20X 500 ml NP0002 10,000 5 L NP0002-02 81,200 NuPAGE Tris-Acetate SDS 20X 500 ml LA0041 9,400 NuPAGE MOPS SDS NP0001, NP0004, NP0005, NP0007 NuPAGE MES SDS NP0002, NP0004, NP0005, NP0007 NuPAGE Tris-Acetate SDS LA0041, NP0004, NP0005, NP0007 1 kit NP0050 13,400 1 kit NP0060 13,400 1 kit LA0050 12,900 NuPAGE 20X 125 ml NP0006 4,000 1 L NP0006-1 24,500 NuPAGE Buffer and Reagent Kit, 1 kit SPNP1000 12,000 XCell SureLock EI0001+ EI9051 Xcell SureLock EI0001 Xcell EI9051 Xcell SureLock NuPAGE NuPAGE NuPAGE 4% 3-8% NuPAGE +TA Buffer* 6% 3-8% NuPAGE +TA Buffer 8% 7% NuPAGE +TA Buffer 10% 10% NuPAGE +MOPS Buffer 12% 10% NuPAGE +MOPS Buffer 14% 12% NuPAGE +MOPS Buffer 16% 12% NuPAGE +MES Buffer 18% 12% NuPAGE +MES Buffer NuPAGE 4-12% 3-8% NuPAGE +TA Buffer 4-12% NuPAGE +MOPS Buffer 4-20% 4-12% NuPAGE +MES Buffer 8-16% 4-12% NuPAGE +MOPS Buffer 10-20% 12% NuPAGE +MOPS Buffer 10% 10% NuPAGE +MES Buffer 16% 4-12% NuPAGE +MES Buffer 12% NuPAGE +MES Buffer 10-20% 4-12% NuPAGE +MES Buffer 18% 12% NuPAGE +MES Buffer 7
Bolt & Bolt NuPAGE 5 ph Bolt Bolt 2 1 Bolt 10 well 40 L 16 A Bolt 1 2 3 4 5 6 7 8 9 10 B B TX 1 2 3 4 5 6 7 8 9 10 2. Bolt 20LGST fusion proteinsbolt B TX WesternBreeze Lane 1: blend of 6 purified proteins Lanes 2 4, 6 7: 250 ng of various GST fusion proteins Lane 5: mix of GST fusion proteins from lanes4 and 6 Lane 8: p53-gst Lane 9: GST Lane 10: MagicMark XP 8
Ordering information Bolt Bolt Welcome Pack A4-12% 10well 1 kit B0412A 68,000 Bolt Welcome Pack B4-12% 15well 1 kit B0412B 68,000 Bolt Welcome Pack + iblot 1 kit B0412AIB1 198,000 Bolt Welcome Pack A Bolt Welcome Pack B Bolt Welcome Pack + iblot Bolt Mini Gel Tank Bolt 4-12% Bis-Tris Plus Gel 10well Bolt 4-12% Bis-Tris Plus Gel 15well Bolt MES SDS Running Buffer20 Bolt LDS Sample Buffer4 Bolt Sample Reducing Agent10 SeeBlue Plus2 Pre-staind Standard iblot iblot 1 10 10 500 ml 10 ml 10 ml 500 L 1 10 B4477599 BG04120BOX BG04125BOX B0002 B0007 B0009 LC5925 IB1001 IB301001 58,000 21,500 21,500 10,000 2,000 8,800 21,000 168,000 22,100 1 2 2 1 1 1 1 1 1 142,800 68,000 142,800 68,000 332,900 198,000 Bolt Bolt 8% Plus 10 wells 10 / BG00080BOX 21,500 Bolt 8% Plus 12 wells 10 / BG00082BOX 21,500 Bolt 8% Plus 15 wells 10 / BG00085BOX 21,500 Bolt 8% Plus 17 wells 10 / BG00087BOX 21,500 Bolt 10% Plus 10 wells 10 / BG00100BOX 21,500 Bolt 10% Plus 12 wells 10 / BG00102BOX 21,500 Bolt 10% Plus 15 wells 10 / BG00105BOX 21,500 Bolt 10% Plus 17 wells 10 / BG00107BOX 21,500 Bolt 12% Plus 10 wells 10 / BG00120BOX 21,500 Bolt 12% Plus 12 wells 10 / BG00122BOX 21,500 Bolt 12% Plus 15 wells 10 / BG00125BOX 21,500 Bolt 12% Plus 17 wells 10 / BG00127BOX 21,500 Bolt 4-12% Plus 10 wells 10 / BG04120BOX 21,500 Bolt 4-12% Plus 12 wells 10 / BG04122BOX 21,500 Bolt 4-12% Plus 15 wells 10 / BG04125BOX 21,500 Bolt 4-12% Plus 17 wells 10 / BG04127BOX 21,500 Bolt Bolt MOPS SDS 20X 500 ml B0001 10,000 Bolt MOPS SDS 20X 5 L B000102 81,200 Bolt MES SDS 20X 500 ml B0002 10,000 Bolt MES SDS 20X 5 L B000202 81,200 Bolt LDS 4X 10 ml B0007 2,000 Bolt LDS 4X 250 ml B0008 18,300 Bolt Sample Reducing Agent10X 250 ul B0004 2,300 Bolt Sample Reducing Agent10X 10 ml B0009 8,800 Bolt Bolt 1 B4477599 58,000 9
LaemmliSDS 3 Large proteins* (116 500 ) 4% 500 Mid-size proteins (20 2) Small proteins (3 ) Wide range (6 200 ) 6% 8% 10% 12% 14% 16% 18% 4 12% 8 16% 4 20% 10 20% 500 2 10% % of length of gel 20% 30% 40% 50% 60% 70% 80% 90% 100% 290 2 166 97 290 2 116 97 66 55 2 2 2 2 2 2 2 2 2 3. * HiMark LC5688 Novex Sharp LC5800, Pre-stained; LC5801, Unstained www.lifetechnologies.com/buyproteingels 10
Ordering information Rhinohide polyacrylamide gel strengthener concentrate, sufficient additive for 1 L of 30% acrylamide/bis-acrylamide37.5:1 200 ml R-33400 22,800 UltraPure TEMED 30 ml 15524-010 3,900 UltraPure Acrylamide 500 g 15512-023 24,600 UltraPure N,N-Methylenebisacrylamide 100 g 15516-024 14,000 UltraPure Tris 1 kg 15504-020 10,300 UltraPure 10% SDS Solution 4 x 100 ml 15553-027 7,300 1 L 24730-020 15,900 UltraPure SDS 500 g 15525-017 19,700 Cassettes, 1.0 mm 25/pack NC2010 18,000 Cassettes, 1.5 mm 25/pack NC2015 17,600 Combs, 1.0 mm, 10 well 25/pack NC3010 5,800 Combs, 1.0 mm, 12 well 25/pack NC3012 5,800 Combs, 1.0 mm, 15 well 25/pack NC3015 5,800 Combs, 1.0 mm, 2D well 25/pack NC3002 5,800 Combs, 1.5 mm, 10 well 25/pack NC3510 5,800 Combs, 1.5 mm, 15 well 25/pack NC3515 5,800 Combs, 1.5 mm 2D well 25/pack NC3502 5,800 Midi& Midi Cassette Combs, 12 + 2 well 10/pack WC1012 9,900 Midi Cassette Combs, 20 well 10/pack WC1020 9,900 Midi Cassette Combs, 26 well 10/pack WC1026 9,900 Denaturing agents UltraPure Urea 2 kg 15505-050 16,100 www.lifetechnologies.com/novexessentials 11
BN-PAGESDS Blue NativeBN-PAGE Coomassie G-250 SDS n- - -D- DDM G-250 2D BlueNative-PAGE 1 4-16% NativePAGE TM Gel CBB stain Western blotting analysis 3-12% NativePAGE TM Gel Western CBB stain blotting analysis () () High Mol. Wt Low Mol. Wt 1048 720 1236 1048 480 242 146 66 480 242 146 66 NativePAGE SDS-PAGE 20 20 Bovine mitochondrial extractnative PAGE 4-16% Bis-Tris SDS-PAGENuPAGE 4-12% Bis-Tris ZOOM SDS-PAGE SYPRO Ruby PVDF NADH-ubiquinone oxidoreductase 12
A B Native 1 2 3 4 5 1 2 3 4 5 1,236 1,048 1,236 1,048 1,048 720 480 720 480 720 480 242 146 66 242 146 66 242 146 66 20 20 20 4. NativePAGE 4 12% A NativePAGE 4 16% BNativeMARK lane 1 0.25%0.5%1.0%2.0%dodecylmaltoside 18 mg lanes 2 5 Colloidal Blue NativeMark Unstained LC0725 NativePAGE 4 16% Bis-Tris Gel Ordering information Native protein analysis BN-PAGE BN-PAGE 1 kit BN1002SP 158,700 BN-PAGE BN/SDS 1 kit BN1004SP 222,500 NativePAGE 3 12% Bis-Tris Gels (10 well) 10 gels BN1001BOX 23,500 NativePAGE 3 12% Bis-Tris Gels (15 well) 10 gels BN1003BOX 23,500 NativePAGE 4 16% Bis-Tris Gels (10 well) 10 gels BN1002BOX 23,500 NativePAGE 4 16% Bis-Tris Gels (15 well) 10 gels BN1004BOX 23,500 NativeMARK Unstained Protein Standard 250 µl LC0725 32,300 NativePAGE Running Buffer Kit 1 kit BN2007 23,000 NativePAGE Sample Prep Kit 1 kit BN2008 23,000 NativePAGE 20X Running Buffer 1 L BN2001 20,000 NativePAGE 20X Cathode Buffer Additive 250 ml BN2002 4,500 NativePAGE 4X Sample Buffer 10 ml BN2003 7,000 NativePAGE 5% G-250 Sample Additive 0.5 ml BN2004 7,100 NativePure NativePure 2 NativePure MammalianNativePure Gateway www.lifetechnologies.com/native 13
260 160 110 80 60 50 40 30 20 15 10 3.5 Novex Sharp Pre-stained LC5800 188 98 62 49 38 28 17 14 6 3 SeeBlue Plus2 LC5925 188 62 49 38 28 18 14 6 3 ~190 ~120 ~85 ~60 ~50 ~40 ~25 ~20 ~15 ~10 460 268 238 171 117 71 55 41 31 260 160 110 80 60 50 40 30 20 15 10 3.5 SeeBlue BenchMark Pre-stained HiMark Pre-stained Novex Sharp BenchMark Mark12 HiMark MagicMark XP LC5625 10748-010 LC5699 LC5801 10747-012 LC5677 LC5688 LC5602 / LC5603 220 160 120 100 90 80 70 60 50 40 30 25 20 15 10 200 116.3 97.4 66.3 55.4 36.5 31 21.5 14.4 6 3.5 2.5 500 290 240 160 116 97 66 55 40 220 120 100 80 60 50 40 30 20 Novex Sharp SeeBlue Plus2 SeeBlue BenchMark HiMark 12 10 9 10 9 12 3 1 2 2 3.5 2 4 2 4 2 10 190 30 4 NuPAGE NuPAGE NuPAGE Ordering information Novex Sharp 2 x 250 µl LC5800 22,000 SeeBlue Plus2 500 µl LC5925 21,000 SeeBlue 500 µl LC5625 21,000 BenchMark 2 x 250 µl 10748-010 19,300 HiMark 250 µl LC5699 27,000 14
Novex Sharp BenchMark Mark12 HiMark NativeMark 12 15 12 9 8 3.5 2 10 2 2.5 200 40 500 20 1,200 3 2050100 2 20 0 0 0 NuPAGE SDS-PAGE NuPAGE SDS-PAGE NuPAGE SDS-PAGE NativePAGE Ordering information Novex Sharp 2 x 250 µl LC5801 20,600 BenchMark 2 x 250 µl 10747-012 19,300 Mark12 1 ml LC5677 18,000 HiMark 250 µl LC5688 24,900 NativeMark 13 250 µl LC0725 32,300 MagicMark XP MagicMark XP IgG 5. A B Ordering information MagicMark XP 50 µl LC5603 9,800 250 µl LC5602 32,000 www.lifetechnologies.com/proteinstandards 15
Coomassie G-2501 SimplyBlue SafeStain SilverQuest Silver Staining Kit SYPRO Ruby Protein Gel Stain Coomassie Fluor Orange Protein Gel Stain Coomassie G-250 UV UV >7 ng BSA 0.3 ng 0.25-0.5 ng 4 8 ng Low Low Low Low 7 70 ng 0.3 10 ng 0.25 1,000 ng 4 1,000 ng Multiplex capability No No Yes No Yes Not recommended Yes Yes Yes Yes Staining membranes PVDF No SYPRO Ruby Protein Blot Stain 12 60 90 3 90 90 30-60 LC6060, LC6065 LC6070 S12001, S12000, S21900 C33250, C33251 No SYPRO Ruby Protein Gel Stain 3 SYPRO Ruby12 6 90SYPRO Ruby 6. SYPRO Ruby Protein Gel Stain 16
Coomassie SimplyBlue SafeStain SimplyBlue SafeStain Coomassie G-250 7 ng 7SimplyBlue SafeStain / 7. SimplyBlue SafeStain SilverQuest Silver Staining Kit SilverQuest Silver Staining Kit SilverQuest 900.3 ng 830 8. SilverQuest Silver Staining Kit Pro-Q Diamond Phosphoprotein Gel Staining Kit Pro-Q Diamond 1-16ng Pro-Q Diamond Stain SYPRO Ruby Stain y MW1 MW2 MW1 MW2 9. Pro-Q Diamond Phosphoprotein Gel Stain Ordering information SimplyBlue SafeStain 1 L LC6060 18,300 3.5 L LC6065 44,000 SilverQuest Silver Staining Kit 1 kit LC6070 36,100 SYPRO Ruby Protein Gel Stain 200 ml S12001 13,000 1 L S12000 43,300 5 L S21900 187,000 Coomassie Fluor Orange Protein Gel Stain 1 L C33250 19,200 Pro-Q Diamond Phosphoprotein Gel Staining Kit 200 ml P33301 23,000 1 L P33300 77,300 Pro-Q Emerald 300 Glycoprotein Staining Kit 1 kit P21857 41,600 17
iblot iblot 7 7 A iblot B 1 2 3 4 5 6 7 8 9 10 1 1 12 1 2 3 4 5 6 7 8 9 10 1 1 12 iblot 1-6 0.0625g, 0.125g, 0.5g, 1.0g, 2.0g, SW480 humn colon cancer cell lysatealexiscontrol 712 5l SellBlue Plus2 Pre-Stained Protein Standard 8-11 0.5l, 1.0l, 2.0l, 4.0l MagicMark XP Western Protein Standard iblot 18
iblot 8 Cu Cu +2 + 2e - 200 Ordering information iblot iblot 1 IB1001 168,000 iblot 8 8 cm : 8 13 cm 2 iblot 8 x 13 cm 10 IB3010-01 22,100 iblot 8 x 8 cm 10 IB3010-02 17,400 iblot PVDF PVDF 8 x 13 cm 10 IB4010-01 24,200 iblot PVDF PVDF 8 x 8 cm 10 IB4010-02 19,000 iblot DNA 8 x 13 cm 10 IB8010-01 22,000 iblot Xcell SureLock 7 7 14 x 8.5 cm 1-3 700 ml 9 x 9 cm 19
BenchPro 4100 BenchPro 4100 2 2 Ordering information BenchPro 4100 1 WP0001 680,000 BenchPro 4100 10 WP1001 16,000 BenchPro 4100 25 ml 50 WP3001 4,800 20
WesternBreeze WesternBreeze ECL Chemiluminescent Kit WesternDot Blotting Kit Qdot 625 BCIP/NBT CDP-Star ECL - pg pg 100 fg pg 100 fg pg pg Autorad/X Autorad/x UV- UV- / Ordering information WesternBreeze WesternBreeze 1 kit WB7103 46,200 WesternBreeze 1 kit WB7105 46,200 WesternBreeze 1 kit WB7107 48,200 WesternBreeze 1 kit WB7104 56,000 WesternBreeze 1 kit WB7106 56,000 WesternBreeze 1 kit WB7108 56,000 WesternBreeze 80 ml each WB7050 14,700 WesternBreeze 16X 2 x 100 ml WB7003 14,700 AP BCIP/NBT 250 ml WP20001 24,000 AP CDP-Star 100 ml WP20002 55,600 AP Nitro Block II 5 ml WP20003 12,600 ECL HRP 1 kit2 x 125 ml WP20005 17,800 2 2 x 100 ml WP20006 10,500 2 2 x 100 ml WP20007 10,500 ECL Novex ECL Chemiluminescent Kits 1 kit WP20005 17,800 WesternDot Blotting Kit WesternDot 625 Goat Anti-Mouse Western Blot Kit WesternDot 625 Goat Anti-Rabbit Western Blot Kit 1 kit 1 kit W10132 W10142 51,900 51,900 21
Pan - CD Cell junctiongap and tight 2,800 Primary Antibody Search Tool gene symbolgene IDconjugate 1 1 Lifetechnologies.com 2 3 Applications Host Conjugation Type Antibody Type Search by Reactivity Predicted Reactivity 22
Qubit 2.0 Fluorometer Qubit 2.0 Fluorometer 1µL 20µL DNARNA W13.3D21.3H4.0cm Qubit Protein Assay 7cm5cm USB CSV 200L 200L * n = + Ordering information Qubit Protein Assay Kit : 0.25-5 g 100 assays Q33211 9,900 500 assays Q33212 32,700 Qubit 2.0 Fluorometer 1 Q32866 170,000 23
Novex www.lifetechnologies.com/novex 201212 The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Coomassie is a trademark of BASF Aktiengesellschaft. Criterion is a trademark of Bio-Rad Laboratories, Inc. 2012, Life Technologies Japan Ltd. All rights reserved. Printed in Japan, 11/2012, PA006-A1212OB www.lifetechnologies.com/tc 108-0023 4-2-8 564-0052 10-28 TEL.0368329300FAX. 0368329580 TEL.0663891201FAX. 0663891206 www.lifetechnologies.com