pp. β- MRI PRE FMRI in vitro FP Chalfie Tsien Design, synthesis and biological application of chemical probes for imaging protein functions Shin Mizukami and Kazuya Kikuchi Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, Yamadaoka, Suita, Osaka, Japan FP Fura- Ca Tsien FMRI
ab FlAsH, ReAsH Griffin et al. HaloTag Los et al. SNAP-tag O - Keppler et al. CLIP-tag SNAP-tag SNAP-tag Gautier et al. Chen et al. Q-tag Lin et al. His-tag Ni -NTA Guignetetal. D -tag Zn Ojida et al. a b kda GFP Da HaloTag kda A β- A β- K β- β-
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MRI HMRI F MRI F H NMR F FMRI F MRI HMRI FMRI FMRI FMRI MRI x y z NMR MRI NMR NMR T T T MRI T MRI T paramagnetic relaxation enhancement PRE PRE MRI a DOTA-DEVD-Tfb Gd-DOTA-DEVD-Tfb F NMR b c- Gd-DOTA- DEVD-Tfb b FNMR c FMRI
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