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- 4 90,000-20 HPLC - UV-Vis ELSD LC/MS - - Agilent 1200 LC (RRLC) 1 RRLC Agilent 1200 LC 2
2 ZORBAX HT 1.8 µm Poroshell 120 Agilent 1200 RRLC HPLC HPLC 20 Poroshell 120 (4 90,000 ) Agilent 1200 RRLC 1200 UV 1200 RRLC 10 300 mm 1.0 4.6 mm 1.5 10 µm Agilent LC HPLC Web www.agilent.com/chem/rrlc_mt 3
1.8 µm HT Agilent 1200 RRLC 100 C HPLC 20 A 10 1 2 3 4 5 1 2 9 8 7 6 2,000 / Agilent 1200 RRLC 2 50 1 2 3 1 3 2 2,000 / Agilent 1200 RRLC : : ZORBAX SB RRHT C18 2.1 mm x 50 mm 1.8 µm : 2.4 ml/min : A - B - : 0-35% B 0.38-95% B 0.46-95% B 0.47-35% B : 95 C : 550 bar : DAD SL 80 Hz [mau] 400 300 200 100 1 2 3 0 0 0.1 0.2 0.3 0.4 4 5 6 1. 0.088-2. 0.139-3. 0.196-4. 0.246-5. 0.264-6. 0.290-7. 0.330-8. 0.364-0. 0.394-7 8 9 197 4
Poroshell 120 Agilent 1200 RRLC 4.6 x 150 mm Poroshell 120 3 60 C 4 90,000 1200 (ELSD) ( ) RRLC UV-Vis Agilent 1200 RRLC UV-Vis 0.4 0.3 11 pg 0.2 1200 SL Plus [mau] 0.1 0-0.1-0.2 (S/N ) 3.2 VWD = 91.2 = 28.5-0.3 0 0.5 1 1.5 2 2.5 3 35 30 25 BSA ZORBAX RRHT SB-C18 (2.1 x 150 mm 1.8 µm) 700 [mau] 20 15 10 5 = 694 LC GC 0 5 10 15 20 25 30 35 40 5
1200 RRLC : : 5 ml/min : 1 ml SL : 120 µl 320 µl 600 800 µl : 0.05 5 ml/min (600 bar) : 4 SL Plus : 0.1 40 µl (1500 µl ) : < 0.25 % RSD (5 40 µl) < 0.5 % RSD (2 5 µl) < 1.5 % RSD (0.5 1 µl) : 2 2 ml x 108 : < 0.004 % SL : 0.1 100 µl (1800 µl ) : < 0.25 % RSD (5 100 µl) < 1 % RSD (1 5 µl) : 2 ml x 100 : 4 40 C CTC HTC PAL : 24 12 2 ml x 324 CTC HTS PAL : 24 18 2 ml x 648 SL Plus : 16 (80) 4 (16) 6 (24) 6
SL : - 10 C 100 C 2 () 2 /6 2 /10 8 /9 2 /6 SL Plus :* ± 0.15 x 10-5 AU 230 nm : 2.5 AU : 160 Hz * SL :** ± 0.8 x 10-5 AU 254 750 nm : 2.0 AU : 80 Hz ** ASTM E 1657-98 SL :** ± 0.8 x 10-5 AU 254 750 nm : 2.0 AU : 80 Hz ** ASTM E 1657-98 RFID LOD: 10 fg (Ex 250 nm Em 400 nm) : 37 Hz : 5 µl/min - 5 ml/min 0.7 6000 LC/MS 6100 Quadrupole LC/MS ( ) 6200 TOF LC/MS 6300 LC/MS 6400 Triple Quadrupole LC/MS 6500 Q-TOF LC/MS 7
RRLC 1 2 Agilent 1200 RRLC RRLC HPLC RRLC 14 15 150 mm 2.1 4.6 mm 140 ZORBAX 1.8 µm RRHT ZORBAX RRHT 3.5 5.0 µm ZORBAX 5.0 3.5 1.8 µm HPLC RRLC LC Poroshell 120 SB-C18 EC-C18 50 150 mm 2.1 4.6 mm [mau] 1400 1200 1000 800 600 400 200 Δt < 2% Δt < 2% 0 0 2 4 6 8 RRLC HPLC 1.0 1.5 10 : : 1100 1200 RRLC ( 600 800 µl) : ZORBAX RRHT 4.6 x 250 mm 5 µm XDB-C18 HPLC Web www.agilent.com/chem/rrlc_mt 8
Agilent 1200 RRLC Agilent 1200 RRLC Agilent 1200 RRLC : 8 15 300 mm 2.1 4.6 mm 6 Agilent ChemStation ( 4 Agilent 6100 LC/MS - 9
ChemStation Agilent ChemStation - Agilent ChemStation - Agilent ChemStation Agilent ChemStation Agilent 1200 RRLC Agilent 6100 LC/MS 5 - (ESA CAD III ) Agilent LC GC CE CE/MS LC/MS A/D Agilent 10
Agilent 1200 2007 LC/GC 1 Agilent EZChrom Elite 44,2% 22,6% 8,6% 8,2% 5,6% 4,8% 3,8% Waters PerkinElmer Thermo Electron Corp Applied Biosystems Shimadzu Scientific Varian SMART / Agilent OpenLAB Agilent OpenLAB 1 1 Agilent Agilent Agilent Agilent ACE ( ) Agilent 11
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