a b Chroma Graphein Chromatography

Similar documents
Oxidative Dimerization of Tocopherols in Saturated and Unsaturated Triglycerides. III Formation of 5-(ƒÂ-Tocopheroxy)-ƒÂ-5-tocopherol and 5-(ƒÂ-Tocoph


ヒト血漿中オキシステロールの高感度分析法

Introduction ur company has just started service to cut out sugar chains from protein and supply them to users by utilizing the handling technology of

T05_Nd-Fe-B磁石.indd

* * 2

CHROMATOGRAPHY, Vol.32 No.3 (2011) Technical Review CoronaCAD Recent Developments in Charged Aerosol Detection Technology and Applications Kyoko Fukus

1998 The Japan Society for Analytical Chemistry 769 Analysis on mass-transfer phenomena in reversed-phase liquid chromatography Kanji MIYABE** **F acu

untitled

Jan THE JAPANESE JOURNAL OF ANTIBIOTICS XL-1 Table 1. Outline of administering doses, routes and sampling times *: 4 ml/hr/kg Bacillus subtilis

00) îSìyçzï®äwÇÃäÓëb-1.pdf


Fig. 3 Coordinate system and notation Fig. 1 The hydrodynamic force and wave measured system Fig. 2 Apparatus of model testing

LC-MS LC-MS NMR NMR LC-NMR [1] LC-NMR LC-NMR LC-NMR NMR [2] LC-NMR MHz NMR HPLC NMR LC-NMR LC-NMR LC-NMR WET water suppression enhanced through T1 eff

Developement of Plastic Collocation Method Extension of Plastic Node Method by Yukio Ueda, Member Masahiko Fujikubo, Member Masahiro Miura, Member Sum

<4D F736F F D F90858C6E5F C B B B838B>

alternating current component and two transient components. Both transient components are direct currents at starting of the motor and are sinusoidal

Table 1. Main specifications of VAD plant. Fig. 2. Typical operating pattern of low alloy steel.

こんにちは由美子です

Waters Sigma-Aldrich 1.0 mg/ml LC Waters Alliance HPLC ACQUITY UPLC H-Class Bio 30 cm Alliance HPLC UV ACQUITY UPLC TUV mm nm XBridge Protein B

日立金属技報　Vol.34

IPSJ SIG Technical Report Vol.2016-CE-137 No /12/ e β /α α β β / α A judgment method of difficulty of task for a learner using simple

<8B5A8F70985F95B632936EE7B22E696E6464>

Introduction Purpose This training course describes the configuration and session features of the High-performance Embedded Workshop (HEW), a key tool

(Shigen to Sozai) Vol.116 p (2000) 石炭灰フライアッシュからのゼオライトのアルカリ水熱合成と生成物の陽イオン交換特性 * 1 1 村山憲弘山川洋亮 2 3 小川和男芝田隼次 Alkali Hydrothermal Synthesis of Zeol


untitled

Fig. 2 Effect of oxygen partial pressure on interfacial tensions between molten copper and fayalite slag (Fe/Si0 2=1.23) at 1473 K. Fig. s Effect or o

δδ 1 2 δ δ δ δ μ H 2.1 C 2.5 N 3.0 O 3.5 Cl 3.0 S μ


Microsoft PowerPoint - LCテクノ　ポスター　塚本

untitled

JOURNAL OF THE JAPANESE ASSOCIATION FOR PETROLEUM TECHNOLOGY VOL. 66, NO. 6 (Nov., 2001) (Received August 10, 2001; accepted November 9, 2001) Alterna

東京都健康安全研究センター研究年報

20 12,, 59 q r Fig.2 [3] Fig.3 1cm Fig.2 Schematic of experimental apparatus for measuring interfacial thermal resistance. Fig.3 Surface morphol


スペースプラズマ研究会-赤星.ppt

xxxxEN.qxd

1..FEM FEM 3. 4.

Studies of Foot Form for Footwear Design (Part 9) : Characteristics of the Foot Form of Young and Elder Women Based on their Sizes of Ball Joint Girth

VOL. 25 NO. 6 CHEMOTHERAPY 1667

植物23巻2号

P036-P041


untitled

橡ﾏﾆｭｱﾙv4c.PDF

42 1 Fig. 2. Li 2 B 4 O 7 crystals with 3inches and 4inches in diameter. Fig. 4. Transmission curve of Li 2 B 4 O 7 crystal. Fig. 5. Refractive index

LC304_manual.ai

1 WSV SIR m/z Analyte RT (Min) SIR m/z Cone voltage (V) Ascorbic Acid (C) Thiamine (B1) Nicotinic Acid (B3) Pyridoxal (

CH 2 CH CH 2 CH CH 2 CH CH 2 CH 2 COONa CH 2 N CH 2 COONa O Co 2+ O CO CH 2 CH N 2 CH 2 CO 9 Change in Ionic Form of IDA resin with h ph CH 2 NH + COO

平成26年度 化学物質分析法開発報告書

I N S T R U M E N T A T I O N & E L E C T R I C A L E Q U I P M E N T Pressure-resistant gasket type retreat method effective bulk compressibility Fro

HPLC カラム 総合カタログ 2015

Fig. 1 Experimental apparatus.

CHEMOTHERAPY APR Fig. 1 Chemical structure of cefotetan (CTT, YM09330)

平常時火災における消火栓の放水能力に関する研究

SPring8菅野印刷.PDF

248 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /-, No./,,.2,/. (,**0) 12 * * * Microencapsulation of Glutamine with Zein by a Solvent Evaporation Metho

The Phase Behavior of Monooleoylglycerol-Water Systems Mivoshi Oil & Fat Co.. Ltd. Faculty of Science and Technology, Science University of Tokyo Inst

第６２巻　第１号　平成２４年４月／石こうを用いた木材ペレット

Fig. 1 Flow diagram of experimental apparatus employed Fig. 2 Porosity change during sulfurization of reduced sample pellets

薬物分析法の開発

微粒子合成化学・講義

Fig. 1. Schematic drawing of testing system. 71 ( 1 )


δf = δn I [ ( FI (N I ) N I ) T,V δn I [ ( FI N I ( ) F N T,V ( ) FII (N N I ) + N I ) ( ) FII T,V N II T,V T,V ] ] = 0 = 0 (8.2) = µ (8.3) G

Figure 1 Detection process of ELSD. All droplets enter the evaporation process Figure 2 Only small droplets enter the evaporation process Nebulization

Note; a: Pressure sensor, b: Semi-permeable membrane, c: O-ring, d: Support screen, e: Solution, f: Solvent. Fig. 2. Osmometer cell. Fig. 1. Schematic

平成26年度 化学物質分析法開発報告書

特-4.indd

untitled

London -van der Waals Coulomb Fe, Mn

Fundamental Study on the SOX Gas Sensor Utilizing Beta-Alumina with Sputtered Praseodymium Oxide Thin Films by Shinya YAO1*, Kenji MIYAGAWA1, Shigeru

Visual Evaluation of Polka-dot Patterns Yoojin LEE and Nobuko NARUSE * Granduate School of Bunka Women's University, and * Faculty of Fashion Science,


9) H. SCHMCLZRIED: Z. Elektrochem. 66 (l%1) p ) W. D. KINGERY et al.: J. Am. Chem. Soc., 42 (1959), p ) F. HUND: Z. Phys. Chem., 199 (195


燃焼圧センサ

<95DB8C9288E397C389C88A E696E6462>

無電解めっきとレーザー照射による有機樹脂板上へのCuマイクロパターン形成

〈論文〉興行データベースから「古典芸能」の定義を考える

原著03_高橋.indd

xEffect_SG_MV_Part2_E_xEffect_SG_MV_E

〈論文〉組織改革の成果に関する予備的調査--社内カンパニー制導入が財務的業績に与える影響


平成26年度 化学物質分析法開発報告書


橡

( ) ( ) 87 ( ) 3 ( 150mg/l) cm cm 50cm a 2.0kg 2.0kg

内容 1. セミ分取 HPLC システム 応用例 留意点 2. 超臨界流体クロマトグラフィー (SFC) を使用した分取の紹介

09_organal2

J. Jpn. Inst. Light Met. 65(6): (2015)


1 2 3

Safe harbor statement under the Private Securities Litigation Reform Act of 1995: This presentation may contain forward-looking statements that involv

平成25年度 化学物質分析法開発報告書

研究成果報告書

The Behaviors of Gluconic Acid Derivative in Hydration of CaO EAl2O3 Naomitsu TSUYUKI, Osamu HISASHI*, Tsugio MIYAKAWA and Junichi KASAI** Department

Salesforce DX.key

<Measurements of Isobaric Boiling Point Curves at High and Low Pressures> Received on July 12, 1968 ** Kazuo Kojima (Dept. Eng. Chem., Nihon Univ., To

Transcription:

a b Chroma Graphein Chromatography

(Stationary Phase) (Mobile Phase)

CHROMATOGRAPHY GAS SFC LIQUID GSC GLC Column Planar NP RP IEC SEC TLC Paper Normal Phase Reverse Phase GPC GFC Thin Layer Chromato.

Normal phase LS Reverse phase LS δ δ+ 30 µ Si - O - H Silica Gel

-COOH -OH -NH 2 C=O -OCH 3 -Cl

Polar solvent Non-Polar solvent

GRAM OF SIMPLE LIQUID COLUMN CHROMATOGRAPHY OH A + B + C OOOOOOOOOO OOOOOOOOOO OOOOOOOOOO OOOOOOOOOO OOOOOOOOOO OOOOOOOOOO OOOOOOOOOO OOOOOOOOOO Solvent(mobile or moving phase) Sample (A+B+C) Column Solid Particles (packing materialstationary phase) OOOOO A OOOO OOOOOOOOOO OOOOOOOOOO OOOOO B OOOO OOOOOOOOOO OOOOOOOOOO OOOOOOOOOO OOOOO C OOOO OOOOOOOOOO OOOOOOOOOO HO HO HO OH O OH Eluant (eluate)

(Normal Phase) (Reversed Phase)

O O O O Si O Si O Si O H O O O O Si O Si O Si O H O O O O O O O Si O Si O Si O H O O O O Si O Si O Si O H O O O (CH 2 ) 17 CH 3 (CH 2 ) 17 CH 3 (CH 2 ) 17 CH 3 : (ODS )

Types of Chromatography MOBILE PHASE LIQUID FORM AT L iquid-l iquid Chromatography (Partition) L iquid-solid Chromatography (Adsorption) STATIONARY PHASE Liquid Solid N ormal Phase Reverse Phase N ormal Phase Reverse Phase M obile Phase - N onpolar Stationary phase - P olar M obile Phase - P olar Stationary phase - N onpolar

FOUR BASIC LIQUID CHROMATOGRAPHY The 4 basic liquid chromatography modes are named according to the mechanism involved: 1. Liquid/Solid Chromatography (adsorption chromatography) A. Normal Phase LSC B. Reverse Phase LSC 2. Liquid/Liquid Chromatography (partition chromatography) A. Normal Phase LLC B. Reverse Phase LLC 3. Ion Exchange Chromatography 4. Gel Permeation Chromatography (exclusion chromatography)

LIQUID SOLID CHROMATOGRAPHY OH HEXANE Si - OH CH 3 - C CH 3 CH 3 OH CH 3 C-CH 3 CH 3 CH 3

Partitioning of the sample between 2 phases delays or retains some components more than others to effect separation. LIQUID-LIQUID CHROMATOGRAPHY ODPN(oxydipropionylnitrile) Normal Phase LLC Reverse Phase LLC NCCH 3 CH 2 OCH 2 CH 2 CN(Normal) CH 3 (CH 2 ) 16 CH 3 (Reverse) The stationary solid surface is coated with a 2nd liquid (the Stationary Phase) which is immiscible in the solvent (Mobile) phase

SELECTING AN OPERATING MODE Sample Type Positional isomers Moderate Polarity Molecules Compounds with Similar Functionality Ionizable Species Compounds with Differing Solubility Mixture of Varying Sized Molecules LC Mode LSC or LLC LSC or LLC LSC or LLC IEC LLC GCC

Schematic Diagram of Liquid Chromatography

Detectors 1. Ultraviolet Detector 200-400nm 254 nm 2. Reflective Index Detector Universal Detector

High Performance Liquid Chromatography

High Performance Liquid Chromatography

Chromatogram of Organic Compounds from Fermented Cabbage

Chromatogram of Orange Juice Compounds

Retention Time Time required for the sample to travel from the injection port through the column to the detector. Response D B A C 5 10 15 20 25 Retention Time

SELECTIVITY (α) Ratio of Net Retention Time of 2 components. (Equilibrium Distribution Coefficient) α = - X 2 X 0 X - 1 X 0

Selectivity Selectivity Response X 2 X 1 X 0 1 3 6 Retention Time

RESOLUTION EQUATION R = V - V 2 1 1/2(W + W ) 1 2 Response V 2 V 1 W W Volumes W 1 2 1 W 2

HEIGHT EQUIVALENT TO A THEORETICAL PLATE Length of a column necessary for the attainment of compound distribution equilibrium (measure the efficiency of the column). X Theoretical plates (N) = 16 ( ) Y 2 X Y

RESOLUTION

EXAMPLES OF THEORETICAL PLATE, SELECTIVITY AND HEIGHT EQUIVALENT TO A THEORETICAL PLATE 2 V 2 4 V 0 V 1 1 3 W 1 W 2 W W 3 4 V 3 V 4 V0 = 1.02(Minutes) V1 = 4.92 V2 = 6.59 V3 = 8.17 V4 = 9.14 W1 = 1.0 (Minutes) W2 =1.0W3 = 1.0 W4 =1.0

GENERAL FACTORS INCREASING RESOLUTION 1. Increase column length 2. Decrease column diameter 3. Decrease flow-rate 4. Pack column uniformly 5. Use uniform stationary phase (packing material) 6. Decrease sample size 7. Select proper stationary phase 8. Select proper mobile phase 9. Use proper pressure 10. Use gradient elution

Distribution Coefficient (Equilibrium Distribution Definition: Concentration of component A in stationary phase Concentration of component A in mobile phase Different affinity of these 2 components to stationary phase causes the separation.

2024 © docsplayer.net プライバシー | 利用規約 | フィードバック