産原27-50（6906）.indd

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1 原著産業動物臨床家畜衛生関連部門要約 PCR PCR BPSV PPV DNA PPV PPV, 材料及び方法発生の概要 : No C 出入口通路通路詰所 B A 出入口 ABC FAX inoshima@gifu-u.ac.jp

2 . A B mm ABC ウイルス学的検査 : Geno Plus Genomic DNA Extraction Miniprep System, Viogene, Taiwan DNA PCR TaKaRa Taq Hot Start Version Inoshima PPV pan parapoxvi

3 No. A B C. rus primerppp PPP RNA RT ReverTra Ace BVDV UTR Vilcek PCR Thermal Cycler, Applied Biosystems, U.S.A. PCR. UV Thermal QIAquick PCR Purification Kit, Qiagen, Germany Cycler Veriti, Applied Biosystems, U.S.A. BigDye 血清学的検査 : Terminator v. Cycle Sequencing Kit, Applied Biosystems, U.S.A. BPSV Chiba DyeEx. Spin Kit, Qiagen, BVDV Nose Germany KZCP MDBKSY Genetic Analyzer, Applied Biosystems, U.S.A. 病理組織学的検査 :No. BVDV HE High Pure Viral RNA Kit

4 BN15 1 : 100 Iwate : 100 Chiba 1 : 100 V660 1 : 100 BN : 200 Iwate : 200 Chiba 101 : 200 V : 200 BN : 300 Iwate : 300 Chiba 201 : 300 V : 300 BN : 400 Iwate : 400 Chiba 301 : 400 V : 400 BN : 500 Iwate : 500 Chiba 401 : 500 V : 500 BN : 554 BPSV : 554 Chiba 501 : 554 V : 554 IwateABChibaAB V AB BN LC No. Nose KZcp A B C 成績ウイルス学的検査成績 :PCR PPV bp PCR BPSV IwateAccession No. ABChibaAB VAB 69.. BPSV BN Bovine Nara LCBVDV RTPCR 血清学的検査成績 : BPSV 326

5 BVDV Nose KZCP 病理組織学的検査成績 : 考察 BPSV BPSV BPSV BPSV Huang BPSV No. 引用文献 Brown CC, Baker DC, Barker IK : Foot-and-mouth disease, Pathology of Domestic Animals, vol., Maxie MG ed, th ed,, Elsevier, Philadelphia () Yaegashi G, Sasaki I, Chiba S, Murakami K : Molecular analysis of parapoxvirus detected in eight calves in Japan, J Vet Med Sci,, () Inoshima Y, Morooka A, Sentsui H : Detection and diagnosis of parapoxvirus by the polymerase chain reaction, J Virol Methods,, () Vilek S, Herring AJ, Herring JA, Nettleton PF, Lowings JP, Paton DJ : Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis, Arch Virol,, () Kuroda Y, Yoshida M, Shibahara T, Matsui T, Nakane T, Hara H, Inoshima Y, Sentsui H : An epidemic of parapoxvirus infection among cattle: Isolation and antibody survey, J Vet Med Sci,, () Kodama K, Sasaki N, Fukuyama S, Izumida A, Ishii F : Studies on cytopathogenic bovine viral diarrhea virus recovery, identification, and properties of the isolated virus, Bull Nippon Vet Zootech Coll,, () Nagai M, Sato M, Nagano H, Pang H, Kong X, Murakami T, Ozawa T, Akashi H : Nucleotide sequence homology to bovine viral diarrhea virus (BVDV ) in the untranslated region of BVDVs from cattle with mucosal disease or persistent infection in Japan, Vet Microbiol,, () Brown CC, Baker DC, Barker IK : Parapoxviral infections, Pathology of Domestic Animalsvol., Maxie MG ed, th ed,, Elsevier, Philadelphia () Iketani Y, Inoshima Y, Asano A, Murakami K, Shimizu S, Sentsui H : Persistent parapoxvirus infection in cattle, Microbiol Immunol,, () Sentsui H, Murakami K, Inoshima Y, Shibahara T, Yokomizo Y : Isolation of parapoxvirus from a cow treated with interferon, Vet Microbiol,, () Huang T, Tulman ER, Diel DG, Khatiwada S, Sims W, Edwards JF, Wen X, Kutish GF, Rock DL, Delhon G : Coinfection with multiple strains of bovine papular stomatitis virus, Arch Virol,, () Mercer A, Fleming S, Robinson A, Nettleton P, Reid H : Molecular genetic analyses of parapoxviruses pathogenic for humans, Arch Virol suppl,, ()

6 Occurrence and Spread of Bovine Papular Stomatitis with Different Types of Lesions Among Japanese Black Calves Suspected of Having Foot-And-Mouth Disease Sayoko EIRAI ), Aki NAKANISHI ), Yukiko YASUKAWA ), Minako MORIYAMA ), Misako KONISHI ) ), ) and Yasuo INOSHIMA ) Nara Prefectural Livestock Hygiene Service Center, 6003 Tsutsui, Yamatokoriyama, , Japan ) National Institute of Animal Health, 315 Kannondai, Tsukuba, , Japan ) Faculty of Applied Biological Sciences, Gifu University, 11 Yanagido, Gifu, , Japan ) The United Graduate School of Veterinary Sciences, Gifu University, 11 Yanagido, Gifu, , Japan SUMMARY Excessive salivation, erosion of the hard palate, and ulcers on the tongue were observed in a four-month-old Japanese black calf that was suspected of having foot-and-mouth disease (FMD). Although FMD was ruled out due to the clinical lesions and epidemiological status over the next two weeks, lesions were observed in ten other calves, ranging from four to seven months in age, at the same farm as well. The lesions included reddening, erythema, papules, erosions, and ulcers in the mouth. Using PCR, bovine papular stomatitis virus (BPSV) was detected in the oral swab samples from eight of the ten affected calves. The nucleotide sequences of the partial envelope gene were identical for all eight samples, and similar to those of the BPSV isolated in Japan. In addition, seroconversion against BPSV was observed in four of the ten affected calves. Based on these results, we diagnosed this as a case of BPSV infection with different types of lesions. Key words : bovine papular stomatitis, foot-and-mouth disease, papules, parapoxvirus, ulcer. Correspondence to : Yasuo INOSHIMA (Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University) 11 Yanagido, Gifu, , Japan TEL FAX inoshima@gifu-u.ac.jp J. Jpn. Vet. Med. Assoc.,, ()

Key words : influenza, nested-pcr, serotype

Key words : influenza, nested-pcr, serotype Table 1 Sequence of PCR primers used to detect HA gene of influenza virus 524 清水 Fig. 1 Detection of PCR product 英明他 (lstpcr) Table 2 Results method Fig. 2