QIAzol Lysis Reagent MaXtract High Density RNAlater RNA Stabilization Reagent QIAGEN QIAzol Lysis Reagent RNA RNase MaXtract High Density QIAzol QIAzo

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Vol.7November 216 3 DNA QIAamp DNA Mini Kit QIAcube QIAamp DNA Mini Kit 2 M Lung Kidney Liver Skin

5 kb QIAamp DNA Mini Kit (5) 5134 25, QIAamp DNA Mini Kit (25) 5136 111, QIAamp DNA Mini QIAcube

3 3 µg DNA QIAcube DNeasy Dellaporta DNA CTAB DNeasy Plant Mini Kit (5) 6914 26,5 DNeasy Plant Mini

1 Vol.7November DNA QIAamp DNA Mini Kit QIAcube QIAamp DNA Mini Kit 2 M Lung Kidney Liver Skin and muscle Whole blood Buffy coat Lymphocytes M QIAcube DNA 48.5 kb QIAamp DNA Mini Kit (5) , QIAamp DNA Mini Kit (25) , QIAamp DNA Mini QIAcube Kit (24) , 5 kb DNA DNA 3 µg 5 kb DNA DNA DNeasy Plant Mini Kit QIAcube DNA Absorbance µg DNA QIAcube DNeasy Dellaporta DNA CTAB DNeasy Plant Mini Kit (5) ,5 DNeasy Plant Mini Kit (25) , DNeasy Plant Maxi Kit (24) ,5 CTAB Wavelength (nm) DNA Dellaporta CTAB DNA DNA 26 nm Sample to Insight

QIAzol Lysis Reagent MaXtract High Density RNAlater RNA Stabilization Reagent QIAGEN QIAzol Lysis Reagent RNA RNase MaXtract High Density QIAzol QIAzol QIAzol Lysis Reagent (2 ml) 7936 38,5 MaXtract

5 ml) 1 5 µl/tube 12946 28,5 MaXtract High Density (2 x 2 ml) 1 75 µl/tube 12956 27,5 MaXtract High Density (1 x 15 ml) 1 6 ml/tube 12965 23,5 MaXtract High Density (25 x 5 ml) 5 2 ml/tube 12973 2,

2 QIAzol Lysis Reagent MaXtract High Density RNAlater RNA Stabilization Reagent QIAGEN QIAzol Lysis Reagent RNA RNase MaXtract High Density QIAzol QIAzol QIAzol Lysis Reagent (2 ml) ,5 MaXtract High Density (2 x 1.5 ml) 1 5 µl/tube ,5 MaXtract High Density (2 x 2 ml) 1 75 µl/tube ,5 MaXtract High Density (1 x 15 ml) 1 6 ml/tube ,5 MaXtract High Density (25 x 5 ml) 5 2 ml/tube , MaXtract MaXtract High Density MaXtract MaXtract RNA RNA RNAlater RNA Stabilization Reagent A C RT B C Number of freezethaw cycles RNAlater RNA Stabilization Reagent RNA RNAlater 2 8 GAPDH RNA A RNA RNA RNA B 2 25 RNA Cat. no. RNAlater RNA Stabilization Reagent (5 ml) 25 x 2 mg ,7 RNAlater RNA Stabilization Reagent (25 ml) 125 x 2 mg ,5 2 Product News Lite Vol. 7/216

3 AllPrep Kits Allprotect Tissue Reagent DNA/RNA/ AllPrep Kits DNA/RNA/ Allprotect DNA RNA RNA gdna gdna RNA AllPrep DNA/RNA AllPrep DNA/RNA Mini Kit (5) 824 6, AllPrep DNA/RNA Micro Kit (5) ,5 AllPrep DNA/RNA/Protein Mini Kit (5) 84 7, AllPrep DNA/RNA FFPE Kit (5) , Allprotect Tissue Reagent 7 12 Fold change in transcript level in vivo.1 2 h 4 h Allprotect 24 h 2 h 4 h PBS 24 h Allprotector DNA Allprotect Reagent RNA 25 Allprotect Reagent PBS Madh7 RT-PCR Allprotect Reagent RNA RNA Allprotect Allprotector 1 DNA RNA Allprotector DNA RNA Allprotector DNA RNA Allprotect Tissue Reagent (1 ml) ,5 Product News Lite Vol. 7/216 3

4 QIAGEN Plasmid Plus Kits Attractene Transfection Reagent Effectene Transfection Reagent DNA QIAGEN Plasmid Plus Kits QIAGEN Plasmid Plus Kit 2 * * * Midi/Maxi 2 Mega/Giga 4 5 pbluescript puc pgem pbr322 EU/µg DNA CompactPrep QIAGEN Plasmid Plasmid Plus S P M DNA QIAGEN Plasmid Plus Midi Kit 2 35 ml 1 25 µg 5 ml 3 1 µg 25/ / ,/127, QIAGEN Plasmid Plus Maxi Kit 8 13 ml <1 mg <2 ml 5 25 µg 25/ / ,/284, QIAGEN Plasmid Plus Mega Kit 5 ml <2.5 mg 5 ml <2.5 mg , QIAGEN Plasmid Plus Giga Kit 2.5 L <1 mg 2.5 L <1 mg , QIAvac 24 Plus 1 24 Spin Column ,5 QIAvac Holder Mega Giga , QIAvac Connecting System 24 VacValves , QIAvac DNA Attractene Transfection Reagent Effectene Transfection Reagent Relative transfection efficiency (%) Attractene Reagent L.4 µg DNA.5 µg DNA with enhancer.5 µg DNA without enhancer Transfection efficiency (1 4 x RLU) Effectene Reagent Reagent L Reagent F Transfection reagent I. Clavereau, D. Petitprez, and I. Van Seuningen, Unité INSERM 377, Place de Verdun, Lille Cedex, France pgfp green fluorescent protein HepG2 FACS Attractene Effectene F9 48 Effectene Attractene Transfection Reagent (1 ml) , Attractene Transfection Reagent (4 x 1 ml) , Effectene Transfection Reagent (4 x 1 ml) , Effectene Transfection Reagent (1 ml) ,5 Effectene Transfection Reagent (1 ml) ,399, 4 Product News Lite Vol. 7/216

TopTaq Master Mix Kit QIAGEN Multiplex PCR Plus Kit QIAGEN OneStep Ahead RT-PCR Kit TopTaq Master Mix Kit 4 3' 5' TopTaq Stabilizer 5' 3' PCR TopTaq Master Mix Kit (25) 243 27, TopTaq DNA Polymerase

5 TopTaq Master Mix Kit QIAGEN Multiplex PCR Plus Kit QIAGEN OneStep Ahead RT-PCR Kit TopTaq Master Mix Kit 4 3' 5' TopTaq Stabilizer 5' 3' PCR TopTaq Master Mix Kit (25) , TopTaq DNA Polymerase TopTaq Stabilizer TopTaq Stabilizer Primer Template DNA PCR QIAGEN Multiplex PCR Plus Kit M 1.25 U 2.5 U 3.75 U 5 U Standard Supplier A II QIAGEN M x Hot Start Taq Factor MP PCR GC 2 Q-solution QIAGEN Multiplex PCR Plus Kit (3) ,5 QIAGEN Multiplex PCR Plus Kit (1) ,5 Fast PCR RT-PCR QIAGEN OneStep Ahead RT-PCR Kit 1 QIAGEN GAPDH Competitor A Competitor B QIAGEN OneStep Ahead RT-PCR Kit (5) ,5 Hela RNA1 1 1 pg GAPDH 1 RT-PCR QIAGEN OneStep Ahead RT-PCR Kit 1 pg RNA QIAGEN OneStep Ahead RT-PCR Kit (2) ,5 QIAGEN OneStep Ahead RT-PCR Kit (2) , Product News Lite Vol. 7/216 5

6 mirneasy Mini Kit Micro Kit PCR mirna lncrna mrna RNA mirneasy Mini Kit Micro Kit 1 QIAcube.8 mirneasy Precipitation mirneasy 18 nt RNA RNA mrna mirna lncrna mrna QIAGEN PCR mirneasy Mini Kit (5)* , mirneasy Micro Kit (5)* ,5 OD RNA Jurkat 1 x 1 6 T mirneasy Mini Kit RNA OD 26 nm 23 nm * mirneasy RT 2 Profiler PCR Array mirna miscript PCR System lncrna RT 2 lncrna PCR System 4 PCR PCR PCR mrna RT 2 Profiler PCR Array mrna PCR lncrna RT 2 lncrna PCR Array lncrna PCR mirna miscript mirna PCR Array microrna PCR 96 2 PCR PCR x 4 1 PCR ,5 86,7 261,5 156,9 111,5 66,9 6 Product News Lite Vol. 7/216

7 mirneasy Serum/Plasma Kit mirna RNA mirneasy Serum/Plasma Kit (5) 2 µl 18nt mirna RNA 2 µl miscript mirna PCR Array mirneasy Serum/Plasma Spike-In Control mir-39 miscript PCR System mirna 14 µl QIAcube A Log1 p B Log p 1.74 Down-regulation Up-regulation RMRP PVT Log 2 NSCLC Log 2 NSCLC Upregulated Unchanged Downregulated Downregulated Upregulated mirna lncrna mirneasy Serum/Plasma Kit non-small cell lung cancer NSCLC 2 µl RNA mirna lncrna NSCLC A Human Serum/Plasma miscript mirna PCR Array B RT 2 PreAMP Human RT 2 lncrna Cancer Pathway Finder PCR Array RNA Application Note mirna qpcr mirna miscript mirna PCR Array Cat. no. mirneasy Serum/Plasma Kit (5) QIAzol C. elegans mir-39 miscript Primer Assay , mirneasy Serum/Plasma Spike-In Control C. elegans mir-39 mirna mimic 1 pmol/tube ,9 Product News Lite Vol. 7/216 7

QIAGEN 39 216 11 3 12 2 QIAGEN QIAGEN Check! www.qiagen.

com/qiagen-club Tel 3-689-73 E-mail epromo-jp@qiagen.

TopTaq (QIAGEN Group); FACS (Becton Dickinson and Company). RNAlater is a trademark of AMBION, Inc.

8 QIAGEN QIAGEN QIAGEN Check! QIAprep QIAGEN QIAGEN QIAprep 2. Spin Column 1 12, Tel epromo-jp@qiagen.com Trademarks: QIAGEN, QIAamp, QIAcube, QIAzol, AllPrep, CompactPrep, DNeasy, Effectene, miscript, TopTaq (QIAGEN Group); FACS (Becton Dickinson and Company). RNAlater is a trademark of AMBION, Inc., Austin,Texas and is covered by various U.S. and foreign patents. Cat. no. 216 QIAGEN, all rights reserved Forefront Tower II Tel: Fax: techservice-jp@qiagen.com /216

AllPrep DNA/RNA Micro プロトコールとトラブルシューティング ( /2008)

AllPrep DNA/RNA Micro プロトコールとトラブルシューティング ( /2008) July 2007 AllPrep DNA/RNA Micro DNA RNA 5 x 10 5 5 mg Sample & Assay Technologies DNA RNA 3 DNA RNA 11 DNA RNA 19 24 2 AllPrep DNA/RNA Micro 07/2007 DNA RNA RNA AllPrep DNA Spin Column DNA RNeasy MinElute