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1 Stratagene Products Mx3000P/Mx3005P PCR

Mx3000P/Mx3005P Mx3000P PCR 5FRET Mx3005P PCR 401403 Mx3000P PCR 401449 Mx3005P PCR SYBR Green I TaqMan PCR

2 Mx3000P/Mx3005P Mx3000P PCR 5FRET Mx3005P PCR Mx3000P PCR Mx3005P PCR SYBR Green I TaqMan PCR PCRThreshold line ROX TM Tm 12Multiple Experimental Analysis) OS 21 CFR part11 96PCR 0.2ml 96 Mx3000P 4 Mx3005P 5 TaqMan SNP 1

3 QPCR Solutions Mx3000P/Mx3005P Mx3000P / Mx3005P PMT 0.2 ml 96 PMT Mx3000P/Mx3005P Mx3000P/Mx3005P PMT PMT Mx3000P/Mx3005P PMT PCR 8 5 Mx3000P Level Take Next Multiplex QPCR to to the 96-well plate 2

4 Mx3000P/Mx3005P 2 ROX TM ROX TM 2 PCR 2 5 1FAM TM HEX TM ROX TM Cy TM 5 ALEXA Fluor 350 DNA5 PCR(ALEXA Fluor Alien, FAM TM - enos, HEX TM -HFE, ROX TM - CFTR, Cy TM 5 - Cyclophilin) Ct Mx3000P/Mx3005P PMT B7U SYBR Green I PCR 9 PCR CGH Mx3000P 4 Mx3005P 5 PCR 3

QPCR Solutions PCR PCR PCR PCR PCR Mx3000P/Mx3005P

(HEX TM DNA (FAM TM Mx3000P/Mx3005P PMT Mx3000P/Mx3005P

HEX TM ROX TM Cy5 TM (%) FAM TM 2278 11 6 3 0.

5 QPCR Solutions PCR PCR PCR PCR PCR Mx3000P/Mx3005P Mx3000P/Mx3005P 2PCR Mx3005P FRET Duplex real-time PCR DNA (HEX TM DNA (FAM TM Mx3000P/Mx3005P PMT Mx3000P/Mx3005P Mx3005P DNA 2 FAM TM HEX TM 2 FAM TM HEX TM Ct S/N FAM TM HEX TM ROX TM Cy5 TM (%) FAM TM % HEX TM % ROX TM % Cy5 TM % 4

6 MxPro PCR SYBR Green I PCR EvaGreen TM mirna SNPs ROX TM Assay name SYBR Green I PCR TaqMan ID Ready-to-Use TaqMan 2 3 PCR PCR PCR Run PCR Adaptive MxPro adaptive Threshold XML instrument data 5

7 QPCR Solutions Mx3000P/Mx3005P MxPro Amplification plot PCR SYBR & Multiplex Dissociation curve Raw data (first derivative) Standard curve Consolidated reports Standard curve ( Genotyping & SNP MxPro ΔΔ Ct Comparative quantification Relative quantity chart ( PCR Multiple Experimental Analysis Multiple Experiment Analysis Threshold line 12 Multiplex Experimental Analysis3 Threshold line IRC Inter-run calibrators 6

Mx3000P/Mx3005P PMT 1 3.5 /96/1 100V 10A 50-60Hz 10 60 µl / 200 µl PCR 96 Mx3000P Mx3005P 4 /1 5 /1 4 5 FRET Ramp Rate 7 10 350 750 nm 350 700 nm 0.25 2.

8 Mx3000P/Mx3005P PMT /96/1 100V 10A 50-60Hz µl / 200 µl PCR 96 Mx3000P Mx3005P 4 /1 5 /1 4 5 FRET Ramp Rate nm nm / cm54 cm45 cm20 kg PC Microsoft Windows Vista Pentium III Mx3000P Mx3005P ALEXA Fluor nm 440 nm FAM TM /SYBR Green I 492 nm 516 nm TET 517 nm 538 nm HEX TM /JOE TM /VIC TM 535 nm 555 nm Cy TM nm 568 nm TAMRA TM 556 nm 580 nm ROX TM /Texas Red 585 nm 610 nm Cy TM nm 665 nm 7

9 QPCR Solutions Mx3000P/Mx3005P Mx3000P Mx3005P SYBR Green I PCR TaqMan PCR PCR 4 5 PCR LNA PCR FRETPCR * * PCR ** ** Tm SYBR Green/ TaqMan SNP SNP SNP Tm PicoGreen /RiboGreen EvaGreen TM mirna FRET * FRET PCR ** Mx3000P/Mx3005P SYBR Green I TaqMan Mx3000P/Mx3005P 8

Agilent 2100 PCR RNA PCR RNA FFPE RNA PCRRNA PCR Agilent 2100RNA

HPRT13' 5' PCRRIN RNA Agilent 2100 SYBR Green I SYBR Green I 2 2

300, 315 bp 100, 105 bp Agilent 2100 900, 1000 bp 473, 500 bp 300,

10 Agilent 2100 PCR RNA PCR RNA FFPE RNA PCRRNA PCR Agilent 2100RNA Chip Agilent 2100 RNA RNA RIN RNA Integrity Number PCR RIN RNA HPRT13' 5' PCRRIN RNA Agilent 2100 SYBR Green I SYBR Green I 2 2 SYBR Green I Tm SYBR Green I Tm 9 marker 900, 1000 bp 473, 500 bp 300, 315 bp 100, 105 bp Agilent , 1000 bp 473, 500 bp 300, 315 bp 100, 105 bp Agilent 2100 Agilent Agilent 2100 Agilent 2100

11 QPCR Solutions Agilent 2100 Lab-on-a-Chip Agilent 2100 Agilent 2100 Labon-a-Chip Chip 7,000 1 DNA RNA Agilent 2100 Chip 21 CFR Part11 1 II DNA II RNA RIN=9.2 RNA RIN= ng DNA II RNA RIN (RNA Integrity Number)RNA 50pg Total RNA II Protein 10

12 PCR SYBR GreenPCR SYBR GreenPCR Brilliant II SYBR Green QPCR Master Mix Brilliant II SYBR Green QPCR Low ROX TM Master Mix Brilliant II FAST SYBR Green QPCR Master Mix 400 SYBR Green 1-Step RT-PCR Brilliant II SYBR Green QRT-PCR Master Mix, 1-Step Brilliant II SYBR Green QRT-PCR Low ROX TM Master Mix, 1-Step 400 SYBR Green 2-Step RT-PCR Brilliant II SYBR Green QRT-PCR AffinityScript Master Mix, 2-Step 400 PCR PCR Brilliant II QPCR Master Mix Brilliant II QPCR Low ROX TM Master Mix Brilliant II Fast Master Mix Step RT-PCR Brilliant II QRT-PCR Master Mix, 1-Step Brilliant II QRT-PCR Low ROX TM Master Mix, 1-step Step RT-PCR Brilliant II QRT-PCR AffinityScript Master Mix, 2-Step 400 PCR Brilliant Multiplex QPCR Master Mix 200 cdna AffinityScript QPCR cdna Synthesis Kit 50 Mx3000P/Mx3005P Mx3000P/Mx3005P Replacement Bulb Assembly Well QPCR Plates, Semi-Skirted Well QPCR Plates, Non-Skirted Mx3000P/Mx3005P Strip Tubes Mx3000P/Mx3005P Optical Strip Caps Single PCR Reaction Tubes (Without Caps) MxP Perfect Fit Frame 1 RNA Stratagene QPCR Reference Total RNA, Human 25 µg Stratagene QPCR Reference Total RNA, Mouse 25 µg MVP RNA 11 Adult and Fetal Human Total RNA and Poly(A)+ RNA Mouse and Rat Total RNA and Poly(A)+ RNA

13 QPCR Solutions SideStepPCR Brilliant SYBR Green SideStep QPCR Master Mix SideStep II SYBR Green QRT-PCR Master Mix, 2-Step SideStep II QRT-PCR Master Mix, 1-Step SideStep II QRT-PCR Master Mix, 2-Step SideStep Lysis and Stabilization Buffer SideStep II Cell Lysate Analysis Kit SideStep II QPCR cdna Synthesis Kit 50 RNA Total RNA Absolutely RNA Miniprep Kit Absolutely RNA Microprep Kit Absolutely RNA Nanoprep Kit Absolutely RNA 96 Microprep Kit 2 RNA Absolutely RNA FFPE Kit Absolutely RNA FFPE Kit 50 RNA Absolutely RNA mirna Kit 50 PCR DNA Micro Extraction Kit StrataPrep DNA Gel Extraction Kit StrataPrep PCR Purification Kit StrataClone Ultra PCR Cloning Kit StrataClone Blunt PCR Cloning Kit StrataClone PCR Cloning Kit 20 mirna mirna QRT-PCR Master Mix kit mirna QPCR Master Mix High-Specificity mirna QRT-PCR Detection Kit High-Specificity mirna QPCR Core Kit mirna 1st Strand cdna Synthesis Kit Escherichia coli Poly (A) Polymerase mirna Specific Forward Primer (200µl) µm PCR MycoSensor QPCR Assay Kit 50 QPCR Alien QRT-PCR Inhibitor Alert Alien Reference RNA QRT-PCR Detection Kit (FAM TM detection) Alien Reference RNA QRT-PCR Detection Kit ( VIC TM detection)

Mx3000P/Mx3005P QPCR System Service Program Mx3000P/Mx3005PPCR 1 1 Mx3000P/Mx3005PPCR 1 401464-1 Mx3000P/Mx3005P 1 401464-2

14 Mx3000P/Mx3005P QPCR System Service Program Mx3000P/Mx3005PPCR 1 1 Mx3000P/Mx3005PPCR Mx3000P/Mx3005P Mx3000P/Mx3005P Mx3000P/Mx3005P 3 * * QC Mx3000P/Mx3005PPCRQC Mx3000P/Mx3005PPCR QC Mx3000P/Mx3005PPCR QC Mx3000P/Mx3005P QC 1 13

QPCR Solutions 21 CFR Part 11 MxPro ET MxPro21 CFR Part11 audit trail 21 CFR Part11 Mx3000P/Mx3005PPCR 401467 MxPro ET Software Activation Mx3000P/Mx3005PPCR 401468 MxPro ET Software Multi-User

15 QPCR Solutions 21 CFR Part 11 MxPro ET MxPro21 CFR Part11 audit trail 21 CFR Part11 Mx3000P/Mx3005PPCR MxPro ET Software Activation Mx3000P/Mx3005PPCR MxPro ET Software Multi-User License Agreement 6 Beacon Designer PCRSYBR Green TaqMan FRETPCR / Beacon Designer / TaqMan Mfold* / Entrez dbsnp BLAST GenBank SNPSNP BLAST * 14

* This is a Licensed Real-Time Thermal Cycler(s) or Licensed Real-Time Temperature Cycling I nstrument(s) under ABI s United States Patent No. 6,814,934 and corresponding claims in non-u.s. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics.

16 * This is a Licensed Real-Time Thermal Cycler(s) or Licensed Real-Time Temperature Cycling I nstrument(s) under ABI s United States Patent No. 6,814,934 and corresponding claims in non-u.s. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed expressly, by implication or by estoppel under any other patent claim. Absolutely mrna, AffinityScript, MxPro, and SideStep are trademarks of Stratagene in the United States. Absolutely RNA, Brilliant, FullVelocity, Mx3000P, and Mx3005P are registered trademarks of Stratagene in the United States. Agilent is a registered trademark of Agilent Technologies. Excel and PowerPoint are registered trademarks of the Microsoft Corporation. SYBR is a registered trademark of Molecular Probes. Scorpions is a trademark of DxS Ltd. TaqMan is a registered trademark of Roche Molecular Systems, Inc. TRI Reagent is a registered trademark of Molecular Research Center, Inc. FAM, TET, HEX, VIC, JOE, TAMRA, and ROX are trademarks of Applera Corporation or it s subsidiaries in the US and certain other countries. Cy 3 and Cy 5 are trademarks of Amersham Biosciences. RiboGreen is a registered trademark of Molecular Probes _japan@agilent.com Stratagene, an Agilent Technologies company, 2009 Printed in Japan, August 7, JAJP Stratagene Products

AriaMx PCR AriaMx PCR PCR AriaMx PCR 0.2 ml 96 6 PCR Ct SYBR Green I PCR Tm TaqMan PCR DNA HRM TaqMan AriaMx 8 Multiple Experiment Analysis PDF RDML P

AriaMx PCR See Deeper. Reach Further. AriaMx PCR AriaMx PCR PCR AriaMx PCR 0.2 ml 96 6 PCR Ct SYBR Green I PCR Tm TaqMan PCR DNA HRM TaqMan AriaMx 8 Multiple Experiment Analysis PDF RDML PCR ROX Rn MIQE