BioWhittaker 108 BME 109 DMEM 109 DMEMF GMEM IMDM 112 L A 11 MEN NCTC RPMI BioWhittaker 1
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1 BioWhittaker 107 BioWhittaker 119 BioWhittaker 141
2 BioWhittaker 108 BME 109 DMEM 109 DMEMF GMEM IMDM 112 L A 11 MEN NCTC RPMI BioWhittaker 120 TheraPEAK MSCGM-CD 121 TheraPEAK FGM -CD 122 UltraCULTURE 12 PC-1, 124 UltraMEM 125 X-VIVO, 126 ProCHO CHO 128 PowerCHO, CHO 129 PowerCHO Advance Medium Chemically Defined 10 ProFreeze -CDM, NAO, 2X 10 UltraMDCK, 11 ProMDCK 2D 11 Pro29, 12 ProVero 1 12 ProPer 1, 1 PERMEXCIS 1 HL-1, 14 UltraDOMA 16 UltraDOMA-PF 17 ProDoma 17 Insect-XPRESS 18 ProNS0, 19 BioWhittaker 142 BSS 1X
3 BioWhittaker BioWhittaker 108 BME 109 DMEM 109 DMEMF GMEM IMDM 112 L A 11 MEN NCTC RPMI / BioWhittaker
4 / BioWhittaker BioWhittaker cgmp DMR DMR DMR WFI the Tissue Culture Association USP FTM TSB FTM TSB TSB FTM ph 2 / Kinetic-QCL
5 (Basal Medium EagleBME) Minimum Essential Medium (MEM) F 12-12A Basal Medium Eagle (BME) Cryoprotective Freezing Medium Earle's BSS L- L- 15% DMSO (11) , ml 7,000 ProFreeze -CDM12-769E (Dulbecco's Modified Eagle's MediumDMEM) DMEM L- HEPES 1.0 g/l 4.5 g/l UltraGlutamine I F 12-7F 12-7Q F F F F F BE12-604F/U F Q F Q / BioWhittaker
6 (Dulbecco's Modified Eagle's MediumDMEM) / BioWhittaker F Q DMEM Dulbecco's Modified Eagle Medium (DMEM) 4.5 g/l L- 4.5 g/l L , , F 4.5 g/l L , Q 4.5 g/l L ,200 BE12-604F/U F F F F F 12-7F 12-7Q F DMEMF12 DMEMF12 F12 DMEM 4.5 g/l UltraGlutamine 4.5 g/l L- 4.5 g/l L- 1.0 g/l L- 1.0 g/l 25 mm HEPES L- 4.5 g/l L- 4.5 g/l L- 4.5 g/l L- 4.5 g/l L , , , , , , , , ,200 L- HEPES.151g/L UltraGlutamine I F Q BE04-687Q BE04-687F/U F.151 g/l 2 8 6, Q L-15mM HEPES 2 8 9,800 BE04-687F/U1 DMEMF g/l Dulbecco's Modified Eagle Medium:F12 UltraGlutamine l (DMEMF12) HEPES 2 8 8,000 BE04-687Q.151 g/ll- HEPES ,
7 (Glasgow Minimum Essential Medium: GMEM) BHK F Glasgow Minimum Essential Medium L ,500 (Grace's Insect Medium) F () F () Grace's Insect Medium (Ham's Medium) CHO yeastolate FBS FBS 2 8 9, , F F F12 Ham's F12 Medium F10 Ham's F10 Medium L , ,500 / BioWhittaker
8 (Iscove's Modified Dulbecco's MediumIMDM) IMDM HEPES F F Q F Q L- HEPES / BioWhittaker F 2 8 7,000 L-25 mm HEPES Q 2 8 9, F Iscove's Modified Dulbecco's Medium 25 mm HEPES 2 8 7, Q (IMDM) L ,00 L-25 mm HEPES F 2 8 7,600 L-15 () (L-15 (Leibovitz) Medium) CO E L-15 2X L-15 Leibovitz's Modified Medium (2X) L- () *L- (1X) (2X) ml 7, F L ,000 L Q L-15 Leibovitz's Medium ,
9 5A (McCoy's 5A Medium) F 5A L-5 mm HEPES 2 8 8, F McCoy's 5A Medium L ,500 MEM (MEM Eagle/E-MEM) MEM () Hank's BBS Earle's BBS MEM-Hanks'12-127F12-17F CO2 L- L- HEPES HBSS EBSS NEAA,, B,, FBS UltraGlutamine I Q (Joklik's) 12-17F F G E (2X) F (10X) 450 ml 100 ml 12-76E 12-76F 100 ml 12-16F 12-16Q F Q F Q F Q BE02-002F F / BioWhittaker
10 MEM (MEM Eagle/E-MEM) F MEM Minimum Essential Medium Alpha Eagle with Earle's BSS L ,500 / BioWhittaker BE02-002F MEM Minimum Essential Medium Alpha Eagle UltraGlutamine I 2 8 8, F 2 8 6,500 L Q 2 8 8, F ,500 L Q , F 2 8 7, Q MEM MEM L ,500 Earle's BSS 12-16F Minimum Essential Medium Eagle 25mM HEPES , Q with Earles BSS L , E ml 6,400 L- 25 mm HEPES 10 µg/ml 12-76F 50 units/ml , µg/ml 2.5 µg/ml B 2.0% FBS MEM Earle's BSS F L-NaHCO ,400 Minimum Essential Medium Eagle with Earle's BSS (10X) E MEM ml 5,900 Earle's BSS L- BE12-668F Minimum Essential Medium Eagle () ,700 with Earle's BSS (2X) L G ml 8,600 MEM MEM Earle's BSS F L ,200 Minimum Essential Medium Eagle with Earles BSS 25mM HEPES 12-17F ,00 L-HEPES Q MEM Minimum Essential Medium Eagle Joklik's Formulation L ,
11 199 (Medium 199) 199 CO F F F Q F L- HEPES HBSS EBSS 1.4 g/l 2.2 g/l F F Q F F 199 Medium 199 Hank's BSSL- 1.4 g/l NaHCO Earle's BSSL- 25 mm HEPES2.2 g/l NaHCO Earle's BSSL- 25 mm HEPES2.2 g/l NaHCO L-25 mm HEPES 1.4 g/l NaHCO Earle's BSSL- 2.2 g/l NaHCO NCTC-109 (NCTC-109 Medium) 2 8 7, , , , ,200 / BioWhittaker 12-92E NCTC-109 NCTC-109 Medium Earle's BSSL ml 8,
12 RPMI 1640 (RPMI 1640 Medium) RPMI MOPS RPMI F / BioWhittaker L- L- HEPES MOPS UltraGlutamine I D- BE12-752F F E F 100 ml F BE12-115F/U1 BE12-115E F Q 100 ml F Q BE12-702F/U F L-165 mm MOPS F Q , E L-25 mm HEPES ml 6, F 100 units/ml 50 µg/ml 2 8 8, F RPMI ,000 RPMI 1640 Medium L-25 mm HEPES Q 2 8 9, F 2 8 5,500 L Q 2 8 7, F L ,000 BE12-752F L-D , F RPMI ,500 L Q RPMI 1640 Medium 2 8 7,
13 04-51Q Schneider's Drosophila Medium L ,400 (William's Medium) BE02-019F E William's Medium E L ,200 / BioWhittaker
14 Notes / BioWhittaker
15 BioWhittaker BioWhittaker 120 TheraPEAK MSCGM-CD 121 TheraPEAK FGM -CD 122 UltraCULTURE 12 PC-1, 124 UltraMEM 125 X-VIVO, 126 ProCHO CHO 128 PowerCHO, CHO 129 PowerCHO Advance Medium Chemically Defined 10 ProFreeze -CDM, NAO, 2X 10 UltraMDCK, 11 ProMDCK 2D 11 Pro29, 12 ProVero 1 12 ProPer 1, 1 PERMEXCIS 1 HL-1, 14 UltraDOMA 16 UltraDOMA-PF 17 ProDoma 17 Insect-XPRESS 18 ProNS0, 19 / BioWhittaker
16 42 / BioWhittaker
17 TheraPEAK MSCGM-CD TheraPEAK MSCGM-CD L- MSCGM-CD Poietics (hmsc) DMEM(10%FBS) Poietics (hmsc) * 84 MSCGM-CD BulletKit MSCGM-CD Mesenchymal Stem Cell Growth Medium BulletKit MSCBM-CD MSCGM-CD Mesenchymal Stem Cell Basal Medium Chemically- Defined MSCGM-CD SingleQuots MSCGM-CD Mesenchymal Stem Cell Growth Medium SingleQuots Supplements and Growth Factors 9, , ,000 / BioWhittaker
18 TheraPEAK FGM -CD TheraPEAK Collagen Production by NHDF-Ad grown in FGM-CD Collagen (µg) Donor l Donor ll Donor lll Donor lv FGM-CD DMEM + 10%FBS / BioWhittaker Fibroblast Ab 5B5 CD90 Collagen I FGM-CD DMEM+10%FBS TheraPEAK FGM -CD BulletKit TheraPEAK FGM -CD Fibroblast Growth Medium BulletKit 29, TheraPEAK FBM -CD TheraPEAK FBM -CD Fibroblast Basal Medium Chemically- Defined TheraPEAK FGM -CD SingleQuots TheraPEAK FGM -CD Fibroblast Growth Medium SingleQuots Supplements and Growth Factors , ,000 *
19 UltraCULTURE UltraCULTURE UltraCULTURE UltraCULTURE mg/ml UltraCULTURE 12-12A UltraCULTURE L- 200 mm L ml UltraCULTURE FDA UltraCULTURE UltraCULTURE HEL, N-10 HeLa HuL-1,2 HuK-1 HuS-1AT HEC HL-60 Raji EB- K-562 HNK HTC29 TT MB21 U18 FMA NS-1 L P88D1 P815 T B82 L RPL-1 RSP-2 RLG-1 Lym-1 RCR , MMQ GC, GH C A77 Rat-1 JTC-12 COS1, COS7 SV40 / BioWhittaker F UltraCULTURE UltraCULTURE Serum-free Medium, general purpose L ,500 L- 144 ProFreeze -CDM, NAO, 2X
20 / BioWhittaker PC-1, PC-1 PC-1 HEPES PC-1 PC-1 HeLa HTB-72 HTB-4 WISH WI-8 HEP2 MRC-5 BHK-21 CHO-K1 NRK C6 T9 ARL6T T STO VERO MDCK SIRC / 7722 PC-1 PC-1 Serum-free Medium for Primary Adherent Cells Chemically Defined () L ,000 L- 144 ProFreeze -CDM, NAO, 2X
21 UltraMEM UltraMEM 24% UltraMEM 10% <2% 1% ITS ITES17-88Z, 17-89Z UltraMEM UltraMEM L- ITES 2 20 µg/ml UltraMEM (%) CEF 2% FBS WI-8 % FBS RHMK 2 4% FBS MRC-5 % FBS RK % FBS BGMK 2 % FBS HEL 2 % FBS CMK 4% FBS VERO % FBS CRFK 2 % FBS L929 4% FBS MDCK 2 % FBS McCOY 4% FBS T 4% FBS CHO-K1 % FBS PK-15 4% FBS MK2 4% FBS / BioWhittaker 12-74F UltraMEM UltraMEM Reduced Serum Medium ITESL ,000 ITES 144 ITS
22 X-VIVO, / BioWhittaker X-VIVO LAKPBL TIL C X-VIVO 10 LAK ril-2/ml 1, /ml PBL X-VIVO 10 LAK ril-21001,000 PBL /ml X-VIVO 1021X X-VIVO 15 X-VIVO 15 X-VIVO 10 TILX-VIVO 15 CD + X-VIVO 15 PBLNK HUT-78 X-VIVO 20 X-VIVO 20 PBL LAK /ml LAK X-VIVO 20PBL TIL Q 04-80Q X-VIVO 10 X-VIVO 10 Serum-free Hematopoietic Cell Medium Chemically Defined L- L , , Q Q X-VIVO 15 X-VIVO 15 Serum-free Hematopoietic Cell Medium Chemically Defined L- L , , Q X-VIVO 20 X-VIVO 20 Serum-free Hematopoietic Cell Medium Chemically Defined L ,
23 X-VIVO, BE02-05Q BE02-054Q X-VIVO 15 X-VIVO 15 Serum-free Hematopoietic Cell Medium Chemically Defined L- L- 2 8, ,000 BE02-055Q X-VIVO 10 X-VIVO 10 Serum-free Hematopoietic Cell Medium Chemically Defined 2 8,000 / BioWhittaker
24 ProCHO CHO CHO ProCHO CHO CHO ProCHO 4 CHO ProCHO 5 CHO ProCHO AT CHO / BioWhittaker Q Q Q ProCHO 4 CHO ProCHO 4 Protein-free CHO Medium ProCHO 5 CHO ProCHO 5 Protein-free CHO Medium 0.1% Pluronic F-68 L- 0,1% Pluronic F68 L- 0.1% Pluronic F-68 L , , ,000 BE02-016Q ProCHO AT CHO ProCHO AT Serum-free Medium for Adherent CHO Cells L ,000 ProFreeze -CDM, NAO, 2X 10 ProHT 100X
25 PowerCHO, CHO CHO CHO PowerCHO 12 CHO CHO PowerCHO CHO PowerCHO ProCHO IgG lgg (ng/ml) 2,500 2,000 1,500 1,000 >90% CHO Q Q Q BE12-776Q PowerCHO 1 CHO PowerCHO 1 Serum-free Medium Chemically Defined PowerCHO 2 CHO PowerCHO 2 Serum-free Medium Chemically Defined PowerCHO CHO PowerCHO Serum-free Medium Chemically Defined PowerCHO GS CHO PowerCHO GS Serum-free CHO Medium Chemically Defined ProCHO 4 HEPESPluronic F-68 L- HEPESPluronic F-68 L- HEPESPluronic F-68 L- HEPESPluronic F-68 L- PowerCHO 1 PowerCHO 2 PowerCHO Commercial supplier A Commercial supplier B Commercial supplier C Commercial supplier D , , , ,000 / BioWhittaker L- 144 ProFreeze -CDM, NAO, 2X
26 PowerCHO Advance Medium Chemically Defined Non-animal Origin Medium PowerCHO Advance CHOPowerCHO Advance Regulatory friendly 2 8 / BioWhittaker Q ProFreeze -CDM, NAO, (2X) ProFreeze -CDM, NAO 2X FBS ProFreeze DMSO 1 DMSO 17.6 ml ml 2X PowerCHO Advance PowerCHO Advance Chemically Defined (NAO) , E ProFreeze -CDM 2X ProFreeze -CD Freeze Medium Chemically Defined (2X) (NAO) ml 25,
27 UltraMDCK, UltraMDCK - MDCK UltraMDCK UltraMDCK MDCK 2 MDCK Q ProMDCK (2D) ProMDCK 2D - MDCKNAO ProMDCK 2D 2D MDCK UltraMDCK UltraMDCK Serum-free Renal Cell Medium Chemically Defined L ,000 PDL / BioWhittaker , (2D) (2D) D DMEM +FBS Competitor S Passage Passage 2 Passage 1 Competitor G ProMDCK Competitor H Passage 6 Passage 5 Passage 4 Passage 8 Passage 7 Competitor L Q ProMDCK (2D) ProMDCK 2D Medium ,
28 Pro29, Pro Pro29 s 29 Pro29 a 29 / BioWhittaker Q Q Pro29 a Pro29 a Serum-free Medium Chemically Defined for 29 Adherent Cells 0.1% Pluronic F-68 Pro29 s Pro29 s Serum-free Medium Chemically Defined for 29 Cells in Suspension ProVero 1 ProVero 1MDCK Vero ProVero 1HEPES Vero 5.0 µg/l rhegf L- L- 144 ProFreeze -CDM, NAO, 2X , ,000 BE02-00Q ProVero 1 ProVero 1 Serum-free Medium L ,800 ProFreeze -CDM, NAO, 2X
29 ProPer 1, ProPer 1 PER.C6 HEPES PER.C6 ProPer 1 PER.C6 BE02-028Q ProPer 1 ProPer 1 Serum-free Medium Chemically Defined PERMEXCIS PER.C6 PCD// Permexcis PER.C6 PER.C6 Permexcis <200 ng/ml Pluronic F-68L- 0.1% Pluronic F-68 L- L- 144 ProFreeze -CDM, NAO, 2X ,000 (PER.C6 ) / BioWhittaker BE02-09Q PERMEXCIS PERMEXCIS Virus Production Medium Chemically Defined L ,800 L- 144 ProFreeze -CDM, NAO, 2X 10 UltraGlutamine
30 HL-1, HL-1 1 ml 0 µg HL-1 HEPES HL-1 HL-1 PX6Ag8.65 Sp2/0-Ag14 HL-1 FBS 100X HL-1 FBS 100X µg /ml / BioWhittaker HL-1 - HL-1 Serum-free Medium for Hybridoma and Hematopoietic Cells Chemically Defined HL-1 FBS 100X HL-1 Fetal Bovine Serum Substitute Chemically Defined (100X) Hank' BSS L- 1.4 g/l NaHCO , ml 17,500 L- 144 ProFreeze -CDM, NAO, 2X
31 HL-1, HL-1 U97 RaJi B MCF-7 (NIH) MCF-7 (MCF) NIH ZR-75 COLO 02 HSR J82 SW 178 SW780 CCL 119 CCL 21 C91/PL T Undesignated Undesignated MOLT- MOLT-4 NAMALWA THP-1 BB88 P815 P88D1 WeHi JLS-V5 70Z- B 70Z/.12 B S49 and variants T RAW09F1.1 T WeHi7 T I-10 EL4 T RL1 T BW5147. T LBRM- T Friend leukemia C57BL6 (C57 X DBA) L5178Y (DBA/2) VERO MDCK CHO K1 () GCL2 / B X B 8A1 CLLC undesignated WI-L2-729-HF2 undesignated LICR-LON-HMY2 HB44 Sp2/0-Ag14 HB45 Sp2/0-Ag14 HB56 NS-1 HB59 NS-1 HB60 PX6Ag NS-1 MI/9..4HL-2 NS-1 MI/ NS-1 ARB PU PX6Ag 8.65 BCS12 PX6Ag 8.65 BCS 2002 PX6Ag 8.65 undesignated NS-1 undesignated PX6Ag 8.65 TIB 175 / S194 TIB 104 / NS-1 TIB 105 / NS-1 TIB 109 / NS-1 TIB 128 / NS-1 TIB 166 / NS-1 TIB 168 / NS-1 RS / PX6Ag 8.65 T / BioWhittaker
32 UltraDOMA UltraDOMA UltraDOMA 0 µg/ml L- UltraDOMA 12-12A UltraDOMA UltraDOMA NS-1 SP-2 (0.5% FBS ) / BioWhittaker 12-72B UltraDOMA UltraDOMA Serum-free Hybridoma Medium L ,500 L- 144 ProFreeze -CDM, NAO, 2X 10 UltraGlutamine
33 UltraDOMA-PF UltraDOMA-PF UltraDOMA-PF UltraDOMA-PF UltraDOMA-PF L- HEPES UltraDOMA-PF NS-1 SP-2 (0.5% FBS ) CHO UltraDOMA-PF F UltraDOMA Protein-free Hybridoma Medium L- (1X) 2 8 9,500 ProDoma / BioWhittaker ProDoma ProDoma ProDoma HEPES BE02-029Q BE02-02Q ProDOMA 1 ProDOMA 1 Serum-free Hybridoma Medium Chemically Defined 0.1% Pluronic F-68 ProDOMA ProDOMA Serum-free Hybridoma Medium Chemically Defined L , ,
34 Insect-XPRESS Insect-XPRESS Spodoptera frugiperdasf9 Sf21 Sf9 Insect-XPRESS /ml Insect-XPRESS L- BEVS Insect-XPRESS 12-12A5050 / BioWhittaker 12-70F Insect-XPRESS 2 8 9,500 L- Insect-XPRESS Protein-free Insect 12-70Q Cell Medium ,000 ProFreeze -CDM, NAO, 2X
35 ProNS0, NS0 NS0 ProNS0 12CD ProNS0 CD NS0 NS0 2 ProNS0 CD ProNS0 12 CD NSO ProNSO lgg (mg/ml) ProNSO 1 CD ProNSO 2 CD 競合 1 競合 2 競合 / BioWhittaker Day 6 mg/ml ProNS0 NS0 IgG ml 200,000 NS0 0 ml 100 rpm Q J ProNS0 1 ProNS0 1 Protein-free Medium Chemically Defined ProNS0 ProNS0 Lipid Supplement Chemically Defined HEPESPluronic L- 5 ml/l , ml 7,000 L- 144 ProFreeze -CDM, NAO, 2X
36 Notes / BioWhittaker
37 BioWhittaker BioWhittaker 142 BSS 1X / BioWhittaker
38 BioWhittaker BioWhittaker cgmp WFI / BioWhittaker
39 BSS (1X) Q F F 10-54F 10-54Q F Q 04-15Q Q 10-54Q F F 10-54F F BSS 1X Hank's Buffered Saline Solution , , , , , ,500 6,500 / BioWhittaker
40 / BioWhittaker BE02-040E 17-88Z 17-89Z C Glucose Solution ITS 500X ITS (500X) ITES 500X ITES (500X) L- L-Glutamine, 200 mm 100 mg/ml ml 29. mg/ml0.85% NaCl ml 17, ml 18, ml 7, C ml 4, F L mm L-Glutamine, 200 mm 29. mg/ml0.85% NaCl , E ml 6, G L- L-Glutamine, Powder g 12, E ml 1, F Lymphocyte Separation Medium, , C MEM 100X MEM Eagle Vitamine Mixture (100X) ml 5,900 MEM 100X 1-114E MEM Non-Essential Amino Acid Solution (100X) 10 mm ml 6,200 BE17-855E 17-61E 15-61I 1-115E ProHT 100X ProHT Supplement (100X) Sodium Bicarbonate Solution, 7.5% Sodium Bicarbonate, Powder 100 mm Sodium Pyruvate Solution (100 mm) (100X) ProCHO (12-766Q12-029Q04-919Q BE02-016Q) ml 5, % ml 4, g 6, g/l ml 5, E 0.4% Trypan Blue, 0.4% Solution 0.85% NaCl ml 4, F 2.5% Hanks' BSS ,000 1:250 10X 2.5% E Trypsin 1:250 (10X) ml 6,
41 BE02-007E /EDTA 10X Trypsin/EDTA (10X) 5 g/l 1250 and 2 g/l Versene (EDTA) 0.5 g/l g/l Versene (EDTA) ml 7, E ml 4, F /EDTA 1X Trypsin/EDTA (1X) ,000 UltraGlutamine 200 mm 200 mm -L- 100X ().L- BE17-605E/U ml 4,200 UltraGlutamine I Supplement, 200 mm (L-) (100X) F (WFI) , Q , D L 170,000 Water for Cell Culture E L 200, F L 56,000 / BioWhittaker
42 / BioWhittaker CC-498 Ascorbic Acid 25.5 mg/ml ml 9,000 CC ml 22,000 9 mg/ml CC-4092 Bovine Brain Extract ml 9,000 CC-4009 CC-4202 CC-4107 CC-4068 CC-4205 CC-42 Bovine Pituitary Extract 00 mm Calcium Chloride 00 mm hegf hegf Human Epidermal Growth Factor hfgf hfgf Human Fibroblastic Growth Factor Human Transferrin NSF-1 NSF-1 Neural Survival Factor-1 1 mg/ml ml 15, mm ml 9, ml 19, ml 1, mg/ml ml 12, ml 16, E B ml 20, µg/ml B 17-86R Amphotericin B ml 7, L 50 mg/ml ml (screw cap vial) 56, Z 50 mg/ml ml (screw cap vial) 7, L ml 10 mg/ml 15 0 Gentamicin Sulfate (screw cap vial) 45, Z 10 mg/ml ml (screw cap vial) 2, Z 50 mg/ml ml (crimp top vial) 7,
43 F E F 100 ml 17-60E 100 ml E H 20 ml 100 ml R ml 5,000 /5,000 µg 10,000 /10,000 µg 20,000 /20,000 µg 25,000 /25,000 µg 25 µg/ml B L F 20,000 20,000 µg /ml 0.85% , E 10, ml 5,500-10,000 µg F Penicillin-Streptomycin Mixture /ml , % 17-60E 5,000 5,000 µg /ml 0.85% 10,000 10,000 µg 25 µg B/ml0.85% 25,000 25,000 µg /ml 29.2 mg L-/ml ml 5, E ml 7,000 - B H Penicillin-Streptomycin-Amphotericin B Mixture ml 5, R - -L- Penicillin-Streptomycin-L-Glutamine Mixture ml,000 / BioWhittaker
44 10-548E ACK 1X ACK Lysing Buffer (1X) , B -- Dextrose-Gelatin-Veronal ,100 / BioWhittaker (Dulbecco's Phosphate Buffered Saline) F (10X) Q (10X) Q F BE15-512D BE15-512F 110 L 150 L 110 L 1 50 L F Q 17-51F 17-51Q F 10X , mm (PO4) Dulbecco's Phosphate Buffered Saline Q (10X) , Q F Q 1X Dulbecco's Phosphate Buffered Saline (1X) 9.5 mm (PO4) 1 g/l 6 mg/l * 9.5 mm (PO4) 9.5 mm (PO4) , , , F 9.5 mm (PO4) , Q 9.5 mm (PO4) ,
45 12-747F Q (10X) F Q HEPES 17-77F HEPES 1M 4, g/l E HEPES Buffer (1M) 100 ml 14,000 10X Q 6,500 Phosphate Buffered Saline (10X) F 1X 67 mm (PO4) 5, Q Phosphate Buffered Saline (1X) 6,00 BE02-017F F E E PBS EDTA Phosphate Buffered Saline EDTA UltraSaline A UltraSaline A 5X Veronal Buffer (5X) Versene EDTA, 0.02% Versene (EDTA), 0.02% ph 7.5 HEPES 0.2 g/l (0.5 mm)dpbs , , ml 1, ml 5,000 / BioWhittaker
46 CF CF 2 = srbc () (srbc) / BioWhittaker = RBC HA HAI HA-HAI HAI HA 0-956J J 0-904J 0-956J 0-957J A Complement Guinea Pig Hemolysin, 5 ml CHICK 24HR RBC RUBELLA (), 5 ml GUINEA PIG COMPLEMENT, 5 ml GUINEA PIG RED BLOOD CELLS 40%, 60% Alsever SHEEP ERYTHROCYTES 40% BLOOD/60%ALSEVERS 5 ml 56, ml 6,
,,,, BioWhittaker 100 102 DMEM 102 DMEMF12 10 10 104 104 104 L-15 105 5A 105 MEN 105 199 107 NCTC-109 107 RPMI 1640 108 109 109 BioWhittaker 112 Thera
,, BioWhittaker 99 BioWhittaker 111 BioWhittaker 1 ,,,, BioWhittaker 100 102 DMEM 102 DMEMF12 10 10 104 104 104 L-15 105 5A 105 MEN 105 199 107 NCTC-109 107 RPMI 1640 108 109 109 BioWhittaker 112 TheraPEAK
,,,, BioWhittaker (DMEM) 90 DMEM:F L-15() 93 5A NCTC CM 95 RPMI BioWhittaker 1 13 BSS
,, BioWhittaker 85 BioWhittaker 99 BioWhittaker 1 BioWhittaker 131 ,,,, BioWhittaker 88 90 (DMEM) 90 DMEM:F1 91 91 9 9 9 L-15() 93 5A 93-93 199 95 NCTC-109 95 CM 95 RPMI1640 96 97 97 97 BioWhittaker 1
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Aqua Way Philic Aqua Way Cation Technology platform Cell-sheet engineering (1) Technology platform Cell-sheet engineering (2) 1) Collection of cells in a form of thin film 2) o suture required for transplantation
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3. 動物細胞を用いた機能性評価 1)PC-12 細胞を用いた神経突起伸長測定法 筑波大学大学院生命環境科学研究科 北アフリカ研究センター 礒田博子, 韓畯奎 はじめに PC-12 細胞 ( 図 1) は,1976 年 Greene らによってラット副腎髄質褐色腫より単離され株化された細胞である. その生存には神経成長因子 (NGF) を必要としないが, NGF により増殖を停止し神経線維を伸長し,
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