Lotus manual 1/1 (Lotus japonicus) Stougaard Lotus japonicus IMAIZUMI-ANRAKU HARUKO Tel 0298(38)8377 address
|
|
|
- とき いんそん
- 5 years ago
- Views:
Transcription
1 Lotus manual 1/1 (Lotus japonicus) Stougaard Lotus japonicus IMAIZUMI-ANRAKU HARUKO Tel 0298(38)8377 address onko@ss.abr.affrc.go.jp (Lotus japonicus) ( in vitro Mesorhizobium loti (3-1) (3-2) (3-2a) (3-2b) Spot inoculation (3-3) inoculation pouch (3-4) root hair curing 1
2 Lotus manual 2/2 ( ( ) *1 *2 2cm 2cm *3 1.5ml *4 1-2cm 8 1cm 1-2cm 16h Light/8h Dark 25 * (3-1) seedling 3 1) 2) 3) FALCONE *1; *2; (B-129 Gifu) 0.12g = approx. 100seeds *3; #500/ KIMIGAYO sandpaper K 120 *4; 2
3 Lotus manual 3/3 seedling (3-1) *5; (B-129 Gifu) 16h Light/8h Dark 3
4 Lotus manual 4/4 1 (standard petal) 1 (wing petals) 2 (keel (Figure 2-1) 2 [INOX No.5, Fontax ] TCPPN(2, 4, 5- trichlorophenoxypropionic acid, ) 70%(v/v) FALCONE2059(14ml) 15-20cm *1 *2 FALCON2059 (Figure 2-3) (Figure 2-2) (Figure 2-2A) stage4 (Figure 2-2B) F1 stage5 stage4 *3 *4 4
5 Lotus manual 5/5 10 (Figure 2-3A) (Figure 2-3B)*5 5mm 10mg/L TCPPN *6 * (Figure 2-4) (Figure 2-3C)*8 *9 *1; *2; F1 *3; *4; *5; *6; *7; *8; stage4 stage5 *9; 1.5cm (Figure 2-5) 3 F1 F1 5
6 Lotus manual 6/6 in vitro seedling (1) seedling (3-1) 2cm 2cm *1 1 2% hypochlorite, 0.02% tween20 *2 100 FALCONE ml 200 FALCONE ml *3 vortex 1 5 *4 shaker 10 *5 vortex 1 5 *6 shaker *7 *8 0.7%(w/v) *9 seedling (a) seedling seedling 9cm *10 seedling *11 (b) (inoculation pouch) seedling seedling 9cm h Light/ 8h Dark 4-5 *12 seedling *13 *1; *2; KANTO Chemical Co., INC. ; 5.0% of active chloline
7 Lotus manual 7/7 90% *3; FALCONE *4; vortex vortex *5; 10 *2 *6; *7; 2 O/N *8; or 2 (c-1) (c-2) (b) (c-2) (4 1 *9; 0.7% (IWAKI KAGAKU Co., LTD) Bacto Agar (Difco) *10; *11; *12; 1 *13; 7
8 Lotus manual 8/8 (3-2) in vitro *0 (3-2a) 150mm 24mm 1 0.9%(w/v) nitrogen-free *1 20ml YEM or TY * ,000 rpm, 2min, 4 2 OD bacteria/200µl *3 (Mesorhizobium loti JRL501) OD 610 = bacteria 2ml *4 200µl (3-1-6a) seedling 1 tube 5 seedling *5 seedling 16h Light/ 8h Dark, growth chamber *0; (Gifu, B-129) M. loti JRL501 growth chamber seedling/bacteria (3-1-6a) seedling bacteria log phase 10 *1; Nitrogen-free B&D Niftal 4 *2; YEM M. loti JRL501 TY YEM 4 *3; bacteria/ tube (yeast extract ) *4; *5; *4 *5 seedling (3-2b) Spot inoculation spot inoculation *0 (EIKEN KIZAI Co., LTD)1 35ml 0.9%(w/v) agar in nitrogen-free *1 Seedling (3-1-6a) seedling seedlings 5cm (MicroporeTM, 3M) 8
9 Lotus manual 9/9 *2 seedlings growth chamber 5-7 YEM or TY ml 12,000 rpm, 2min, µl *3 50µl (Pilot Black ink) (inoculation mixture) (Sea Plaque (R) GTG (R) Agarose, FMC Bio Products) 1%(w/v) 50µl 25µl *4 (2) *5 white tip µl (4) 10µl EH zone (emerging root hair zone; root hair *6 *7 *8 16h Light/ 8h Dark, growth chamber *0; Nod factor *1; *2; *3; Spot inoculation OD 610 = ml 200µl *4; Melt Temp.=65, Gel Temp.=26-30 *5; Spot inoculation *6; EH zone EH zone (a) (20 seedling (b) 2 *7; tip EH zone tip EH zone 9
10 Lotus manual 10/10 1 3µl *8; (3-3) Inoculation pouch (Leonard jar) (2-2) 1 1 inoculation pouch autoclavable inoculation pouch (CUL-JAR300; IWAKI Lab. Ware) KEEPER series (100mm 115mm; IWASAKI Industry INC.)*1 inoculation pouch 100% (50/50) *2 inoculation pouch inoculation pouch cm (a)1 inoculation pouch 8mm (10cm ) (inoculation pouch 3-4 (b) 6-7 *3 inoculation pouch autoclave Seedling (3-1-6b) seedling 1pouch 16seedlings 40seedlings *4 150ml YEM 500ml , 160rpm 300ml 7,000rpm, 10min, ml nitrogen-free *5 seedlings inoculation pouch 1 20ml 50ml growth chamber (16h Light/ 8h Dark, ) seedlings *6 pouch *1; autoclave *2; (50/50) 7-8mm 10m,
11 Lotus manual 11/11 *3; autoclave *4; 5-14 seedling *5; Inoculation pouch OD 610 = ml ml *6; pouch seedling (3-4) root hair curing Nod factor root hair curling Staining jar, Kartell 355, autoclavable mm 15min 3 80 seedling (3-1-6a) seedling *1 nitrogen-free *2 growth chamber (16h Light/ 8h Dark, ) 3-5 Nod factor (1-1.5cm) nitrogen-free Nod factor *3 growth chamber (16h Light/ 8h Dark, ) *4 *1; Seedling 1cm 0.5cm *2; *3; cells/ml medium Nod factor M root hair curling *4; Nod factor 6 11
12 Lotus manual 12/12 4 YEM (for Rhizobium) K2HPO4 MgSO4.7H2O NaCl Mannitol Na-Gluconate Yeast Extract 0.5 g/l 0.2 g/l 0.1 g/l 5.0 g/l 5.0 g/l 0.5 g/l Adjust to ph6.9 After autoclave, add 16% CaCl2 1µl/ml B&D Nitrogen-free nutrients (for Lotus japoicus) Stock sol. Chemical Stock sol. conc. g/l Final conc. A CaCl2.2H2O 2.0M mM B KH2PO4 1.0M mM C Fe-citrate 0.02M µM D MgSO4.7H20 0.5M mM Add 1ml stock solotuions to 2L of H2O Adjust to ph6.8 K2SO4 0.5M mM MnSO4.H20 2mM µM H3BO3 4mM µM ZnSO4.7H20 1mM µM CuSO4.5H2O 4mM µM CoSO4.7H2O 0.2mM µM Na2MoO2.2H2O 0.2mM µM 1/2 Nitrogen-free nutrients (Niftal) Stock sol. Chemical Stock sol. conc. g/50ml Final conc. 1 CaCl2.2H2O 2.0M mM 2 KH2PO4 1.0M mM 3 Fe-EDTA 0.02M mM 4 MgSO4.7H20 0.5M mM K2SO4 0.5M mM MnSO4.H20 2mM µM 5 H3BO3 4mM µM Add 1ml stock solotuions to 4L of H2O ZnSO4.7H2O 1mM µM CuSO4.5H2O 0.4mM µM CoCl2.6H2O 0.2mM µM Na2MoO2.2H2O 0.2mM µM 12
13 Lotus manual 13/13 Adjust to ph growth chamber (Spot inoculation) EYELA (FLI-301N) 5 80µE sec -1 m -2 (25,000Lx) growth chamber (in vitro inoculation in glass tube) BIOTRON LH-100-RDS 1 30µE sec -1 m µE sec -1 m -2 13
橡ミヤコグサ形質転換法・秋山
Page 1 2000 9 akiyama@biochem.osakafu-u.ac.jp VA 4 1999 9 2000 2 Plant J., 2, 487-496 (1992) Handberg K. and Stougaard J. Exp. Bot., 48,1357-1365 (1997) Stiller J. (Gresshoff PM) 4 80 5 10 20 ( ) < ecotype
温泉の化学 １
H O 1,003 516 149 124 2,237 1974 90 110 1km 2,400 ( 100 Mg 200 (98 ) 43,665 mg 38,695 mg 19,000 mg 2000 2000 Na-Ca-Cl 806 1970 1989 10 1991 4 ph 1 981 10,000 1993... (^^; (SO_4^{2-}) " " 1973-1987 1970
まえがき
JAAS0012006 1 -------------------------------------------------------------------------------------------------------- 1 2 --------------------------------------------------------------------------------------------------------
030801調査結果速報版.PDF
15 8 1 15 7 26 1. 2. 15 7 27 15 7 28 1 2 7:13 16:56 0:13 3km 45 346 108 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 3.10 3.11 3. 3.1 26 7 10 1 20cm 2 1 2 45 1/15 3 4 5,6 3 4 3 5 6 ( ) 7,8 8 7 8 2 55 9 10 9 10
0.45m1.00m 1.00m 1.00m 0.33m 0.33m 0.33m 0.45m 1.00m 2
24 11 10 24 12 10 30 1 0.45m1.00m 1.00m 1.00m 0.33m 0.33m 0.33m 0.45m 1.00m 2 23% 29% 71% 67% 6% 4% n=1525 n=1137 6% +6% -4% -2% 21% 30% 5% 35% 6% 6% 11% 40% 37% 36 172 166 371 213 226 177 54 382 704 216
10 117 5 1 121841 4 15 12 7 27 12 6 31856 8 21 1983-2 - 321899 12 21656 2 45 9 2 131816 4 91812 11 20 1887 461971 11 3 2 161703 11 13 98 3 16201700-3 - 2 35 6 7 8 9 12 13 12 481973 12 2 571982 161703 11
2010 1 1 7 2 16 2 2 06in 3 3 錆 錆 06in 4 4 0.1 5 5 HCl Cl NaOH H Cl O 2 H H 2 O NaCl Na OH H 2 O NaCl 2010 6 6 ph3.0 30 mg/l 30 1 O-157 H7 2010 7 7 100 Cl 80 60 40 20 HClO ClO CL ph 10 100,000ppm 12 01
論文
*, 1 1 Selection of superior Frankia spp. for rapid cultivation of Alnus hirsuta seedlings Maki Saito *, 1 and Mikio Hasegawa 1 4 2 4 28 9 30 cm 30 cm 70 % 5 Abstract: In order to develop the rapid cultivation
CH 2 CH CH 2 CH CH 2 CH CH 2 CH 2 COONa CH 2 N CH 2 COONa O Co 2+ O CO CH 2 CH N 2 CH 2 CO 9 Change in Ionic Form of IDA resin with h ph CH 2 NH + COO
CH 2 CH CH 2 CH CH 2 CH CH 2 CH 2 COONa CH 2 N CH 2 COONa O Co 2+ O CO CH 2 CH N 2 CH 2 CO 9 Change in Ionic Form of IDA resin with h ph CH 2 NH + COOH COOH COOH COO - CH 2 NH + + CH 2 NH COO - COO - COO
平成26年度 化学物質分析法開発報告書
2,2-2,2 -Iminodiethanol Diethanol amine CAS 111-42-2 C 4 H 11 NO 2 105.1356-105.0790-28 C 1) 1.0881 g/cm 3 1)
48 13 13 13 38 21 13 3 13 3.1 l l l l l l 4.1.1 5mm 0.5mm 5mm 10 500mL 500mL 500mL 20 1 200 / 4 5 6 3000G 20 G1118 R N 2 10-8 GG Rcm Nrpm 1μm 2 3000G 1 /1 4.1.2PCB PCB 5mm 0.5mm 5mm 10 500mL 500mL 500mL
後期化学_01_濃度
2011 ( ) 1 4 1 100 g g % * 1 100 g 1 g 1 % * 2 (1 g)/(100 g) = 0.01 = 1 % 100 g 100 g 99 g 100 g 1 g 1 % 2.5 g 50 g (2.5 g)/(50 g) = 0.050 = 5.0 % 100 ml g 1 %(w/w) wt% % %(w/v) % / 2 % SI 2 / % 100 ml
取扱説明書[N906i]
![取扱説明書[N906i]](/thumbs/39/20198420.jpg "取扱説明書[N906i]")
237 1 dt 2 238 1 i 1 p 2 1 ty 239 240 o p 1 i 2 1 u 1 i 2 241 1 p v 1 d d o p 242 1 o o 1 o 2 p 243 1 o 2 p 1 o 2 3 4 244 q p 245 p p 246 p 1 i 1 u c 2 o c o 3 o 247 1 i 1 u 2 co 1 1 248 1 o o 1 t 1 t
外因性内分泌攪乱化学物質調査暫定マニュアル（水質、底質、水生生物）
N- 1) 2) 1) GC/MS HPLC GC/MS 2) 0.01~0.05µg/l 0.5~50µg/kg GC/MS SIM GC/MS SIM GC/MS SIM a) b) -d 10 -d 10 -d 12 -d 12 c) d) 600 C 4 e) 1 99.999% f) a) b) - IX-1 - c) (ODS) 200-1,000mg 5ml 5ml 5ml d) e)
untitled
1 2 3 4 Alteration of program parameters Effect Increase hybridization temperature Can prevent false positive signals, decrease background, can also increase dynamic range Decrease hybridization temperature
HPLC 1M KH 2 PO 4 ph ml Anthranilic acid: AnA 600 ml ml AnA 1.0 ml / min Anthranilic acid= PRESSURE 140 kgf / cm 2
15 10-1-3. 1. HPLC 1M KH 2 PO 4 ph 3.0 50 ml Anthranilic acid: AnA 600 ml 1-1. 350 ml AnA 1.0 ml / min Anthranilic acid=137.14 PRESSURE140 kgf / cm 2 TOSOH TSK-GEL ODS- 80 Ts 1) mg / ml AnA in (φ4.6 250
平成26年度 化学物質分析法開発報告書
2,2-2,2 -Iminodiethanol Diethanol amine CAS 111-42-2 C 4 H 11 NO 2 105.1356-105.0790-28 C 1) 1.0881 g/cm 3 1)
各位 平成１７年5月13日
9000 1 6 7 8 8 9000 1960 1 2 2 3 3 1471 4 1362 5 2006 6 7 8 1967 9 1988 1988 10 1000 1348 5000 3000 2 11 3 1999 12 13 14 9000 A 15 9000 9000 9000 10000 16 6000 7000 2000 3000 6800 7000 7000 9000 17 18
3 1 2
Agilent 4200 TapeStation Agilent 4200 TapeStation 3 1 2 2 3 ScreenTape 3 Agilent 4200 TapeStation QC Agilent 4200 TapeStation Ready-to-use ScreenTape 8 PCR 96-well plate 1 96 1 qrt-pcr DNA RNA DNA / RNA
ブラキポディウム Bd21 形 質 転 換 の 手 順 Bd21 種 子 を 濾 紙 上 に 播 種 *1 4 dark 4 日 ~1 週 間 *1 <ブラキポディウムの 育 成 > 参 照 25 16-h Light で 育 成 3~4 日 間 実 生 を 土 壌 ポットに 植 え 替 え 22
ここに 公 開 する ブラキポディウム Bd21 形 質 転 換 の 方 法 は 理 化 学 研 究 所 バイオマス 研 究 基 盤 チームの 舞 岡 ブランチ( 横 浜 市 立 大 学 木 原 生 物 学 研 究 所 内 )で 実 施 している 方 法 です なお プロトコールを 作 成 するにあたり Sílvia C Alves, Barbara Worland, Vera Thole, John
Chromatin Immunoprecipitation (ChIP)
Chromatin Immunoprecipitation (ChIP) 山根美穂 020617 調整する試薬類 11 % Formaldehyde Solution Formaldehyde* 11 % (v/v) 100mM EDTA-Na (ph 8.0) EGTA-Na 0.5mM 50mM *use 16 % Formaldehyde (Methanol free) (PSI/ コスモバイオ
ml DNA DNA
ml DNA DNA ml PH HO DNADNA DNA LED LED CD CD ch 概 概 mll IC LED OK CD CDDVD CD LED LED LED The LED PVA Part LED LED PPT V LED AI LED V AlCu Fe Al Al PVA ml 概 概 CD CD OHP etc cm PET cm PET cm PET LED LED
無印良品 2012 自転車 カタログ
26 897895321,000 140cm 76.0cm 16.0kg H LED 3 263 897896025,000 140cm 76.0cm 16.5kg H 3 LED 20 8978984 8978977 19,800 134cm 73.0cm 15.0kg LED 2620 2620 8486656550 5536207483 14512372,100 8279999840 26 77342561,417
H22応用物理化学演習1_濃度.ppt
1 2 4/12 4/19 4/27 5/10 5/17 5/24 5/31 (20 ) (20 ) (10 ) (50 ) 3 (mole fraction) X = (mol) (mol) i n 1, n 2,, n x N i X i = n i = n i n 1 + n 2 + + n x N 4 (molarity, M) 1 dm 3 ( L) (mol) (mol/l) = 1 L
TEL:03-5253-1111 2478 TEL:03-3595-2337 FAX:03-3503-7964 Shigella sonnei 200g 300 500cm 0.2 0.3mm 25g 0.45 m Shigella sonnei 3mL 35 37 18 ml 0.1 ml 10 10 ml 40mL 0.1 200 L 25g 1 225mL 35 37 18 24 Shigella
20 57
56 20 57 58 59 12 60 ph 61 62 CCA 63 64 ( 1) 700 7.5 86% 54 17 71 2) 700 6.8 86% 50 15 65 3) 600 700 5.7 84% 36 14 50 550 650 4.2 80% 21 6 27 40 500 3.6 78% 16 7 23 3.6 78% 16 7 23 400 700 4.0 78% 18 7
Microsoft Word - 14_LCMS_アクリルアミド
3--2-3-Iodo-2-propynyl butylcarbamate (IPBC) Carbamic acid, butyl-, 3-iodo-2-propynyl ester Iodocarb CAS 55406-53-6 C 8 H 12 NO 2 I C (g/cm 3 ) (mmhg) log P ow 281.09 (280.9910) 64 68 1.51 1.57 (20 C)
CHEMOTHERAPY APR Fig. 1 Chemical structure of cefotetan (CTT, YM09330)
CHEMOTHERAPY APR. 1982 Fig. 1 Chemical structure of cefotetan (CTT, YM09330) VOL.30 S-1 CHEMOTHERAPY Fig. 2 Comparison of standard curves of CTT on various test organisms by cylinder plate method Column
<4D F736F F D F90858C6E5F C B B B838B>
Isobutyl alcohol IUPAC 2-methylpropan-1-ol 2--1-2-Methyl-1-Propanol, Isobutanol CH 3 H 3 C OH CAS 78-83-1 C 4 H 10 O log P ow ( C) ( C) (kpa) (g/l) 74.12 74.14 1) -108 2) 108 2) 1.2 2) 87 2) 0.8 2) (74.1214)
L- 19 L L- L-
0131001 15 1 31 49 35 145 14 5 19 4 23 15 3 10 7 15 634 1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 L- 19 L- 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49
後期化学_04_酸塩基pH
2011 ( ) ph H3O + H + H3O + HCl H3O + HCl + H2O H3O + + Cl HCl H + + Cl OH OH NaOH OH NaOH Na + + OH NH3 OH NH3 + H2O NH4 + + OH 1 H + OH H + H + * 1 NH3 HCl NH4Cl NH4Cl NH3 + Cl NH3 + HCl NH4 + + Cl.
平成26年度 化学物質分析法開発報告書
N,N- N,N-Dimethylacetamide Acethyldimethylamine CAS 127-19-5 C 4 H 9 NO 87.12 ~ 87.14 87.68413 163-165 C 1) - 18.59 C 2) 1 mg/l 25 C 3) 3.3 hpa 2 C 4) log P ow -.77 2).9429 2/4 C 1) 3.1 4) 1.31E - 8 atm-m
56cm 1 15 1960 2 8 2 2 1 2008 1992 2 1992 2 3562mm 3773mm 2 1980 1991 2008 2007 2003 5 2 3 2003 2005 2008 2010 2005 2008 2012 2010 2012 4 7 4 5 2 1975 1994 8 2008 NPO 2 2010 3 2013 2016 3 2008 2009 14
QIAquick Spin プロトコールとトラブルシューティング ( /2008)
March 2008 QIAquick Spin QIAquick PCR Purification Kit PCR 100 bp 10 kb QIAquick Nucleotide Removal Kit 17 40mer DNA 40 bp 10 kb QIAquick Gel Extraction Kit DNA 70 bp 10 kb Sample & Assay Technologies
09_organal2
4. (1) (a) I = 1/2 (I = 1/2) I 0 p ( ), n () I = 0 (p + n) I = (1/2, 3/2, 5/2 ) p ( ), n () I = (1, 2, 3 ) (b) (m) (I = 1/2) m = +1/2, 1/2 (I = 1/2) m = +1/2, 1/2 I m = +I, +(I 1), +(I 2) (I 1), I ( )
untitled
-1- -2- -4- -5- 20 30 12-6- 8 15 1-7- -8- -9- -10- 40 60-11- -12- 3 4 70 90-13- -14- 2 4 3 5-15- a 3-16- -17- -18- 128 kg 150 200g 100 200g 250 750g 2 1 94 89 89 81 72 390 97 97 83 83 75 97 97 100 86
2007 1 1 1 7 52 SS 52 1977 30 1955 8 1 1 2 3 1 1
2007 1 1 1 7 52 SS 52 1977 30 1955 8 1 1 2 3 1 1 2 1 3 32 1957 4 12 1 4 31 1956 3 3 11 32 SS 1955 8 3 1957 11 SS SS 24 1949 SS SS 20 1945 20 2 1 1 1 SS 1 2 SS SS 1 SS HI BHI HI HI 1930 Difco HI BHI BHI
64 3 g=9.85 m/s 2 g=9.791 m/s 2 36, km ( ) 1 () 2 () m/s : : a) b) kg/m kg/m k
63 3 Section 3.1 g 3.1 3.1: : 64 3 g=9.85 m/s 2 g=9.791 m/s 2 36, km ( ) 1 () 2 () 3 9.8 m/s 2 3.2 3.2: : a) b) 5 15 4 1 1. 1 3 14. 1 3 kg/m 3 2 3.3 1 3 5.8 1 3 kg/m 3 3 2.65 1 3 kg/m 3 4 6 m 3.1. 65 5
2 7 ( ) µ 2
1 2 3 4 5 (written by Dr. ) Stock Solution (one liter) in 4 Buffer A: 0.05 M glucose 20% (1.1 M) glucose 22.7 ml 0.025 M Tris-Cl ph8 1 M Tris (ph8.0) 12.5 ml 0.01 M EDTA 0.5 M EDTA 10 ml Buffer B: 5 M
橡Taro9-表紙、仕切.PDF
1.1 23 1.2 24 1.3 25 26 2.1 2.1.1 (a) (b) (c) (d) (e) (f) (g) (h) () () () () () () () () 27 (a) (b) (c) (d) 2.1.2 2.1 2.1 2.1.3 1 (2) (3) 2.2 2.2 5 0.5 5 10 0.52 10 0.5210 0.5 2 10 2.1.4 (1) (2 28 2.1.5
untitled
. 23 3 2 1 2 3 21 21 22 9 1 23 18 4 5 6 5 40 20 7 1 1 8 9 10 11 12 50cm 13 10 11 14 2011 3 11 15 16 ml mm tel 079-557-0039 & fax 079-557-1888 17 248-240- 98-48- 170-98- 98- ml 85-1,085-18 19 20 21 22 23
(1.2) T D = 0 T = D = 30 kn 1.2 (1.4) 2F W = 0 F = W/2 = 300 kn/2 = 150 kn 1.3 (1.9) R = W 1 + W 2 = = 1100 N. (1.9) W 2 b W 1 a = 0
1 1 1.1 1.) T D = T = D = kn 1. 1.4) F W = F = W/ = kn/ = 15 kn 1. 1.9) R = W 1 + W = 6 + 5 = 11 N. 1.9) W b W 1 a = a = W /W 1 )b = 5/6) = 5 cm 1.4 AB AC P 1, P x, y x, y y x 1.4.) P sin 6 + P 1 sin 45
untitled
NO. 2007 10 10 34 10 10 0570-058-669 http://www.i-nouryoku.com/index.html (40 ) () 1 NO. 2007 10 10 2.2 2.2 130 70 20 80 30 () () 9 10 () 78 8 9 () 2 NO. 2007 10 10 4 7 3 NO. 2007 10 10 40 20 50 2 4 NO.
untitled
-- -- -3- % % % 6% % % 9 66 95 96 35 9 6 6 9 9 5 77 6 6 5 3 9 5 9 9 55 6 5 9 5 59 () 3 5 6 7 5 7 5 5 6 6 7 77 69 39 3 6 3 7 % % % 6% % % (: ) 6 65 79 7 3 36 33 9 9 5 6 7 3 5 3 -- 3 5 6 76 7 77 3 9 6 5
, 360ml P , 360ml P , 360ml P , 40, 720ml P , 14, 2
3-1 3-1 20 2600, 20, 360ml P.35 3-2 3-2 16 2700, 16, 280ml P.35 3-3 3-3 20 2600, 20, 360ml P.35 3-4 3-4 19 2600, 19, 340ml P.35 3-5 3-5 20 2900, 20, 360ml P.35 3-6 3-6 18 3000, 18, 320ml P.35 3-7 3-7 18
PrmryCellCltrガイド_0620.indd
初代細胞培養製品ガイド HEK HEKa C-005-5C [1Vial (>500k cells)] HEKa-APF C-021-5C [1Vial (>500k cells)] Epilife in vitro 2 Epilife 60µM CaCl 2 HEKn C-001-5C [1Vial (>500k cells)] HEKn-APF C-020-5C [1Vial (>500k cells)]
RT-PCR プロトコール.PDF
Real -Time RT-PCR icycler iq Bio Rad RT-PCR RT-PCR 1 icycler iq Bio Rad icycler iq 30 2 Ready-To-Go T-Primed First-Strand Kit (amersham pharmacia biotech) Ready-To-Go T-Primed First-Strand Kit QuantiTect
Introduction ur company has just started service to cut out sugar chains from protein and supply them to users by utilizing the handling technology of
Standard PA-Sugar Chain Catalogue Masuda Chemical Industries Co., LTD. http://www.mc-ind.co.jp Introduction ur company has just started service to cut out sugar chains from protein and supply them to users
untitled
Kw 1-12 1-13 Kw van t Hoff 0 0 0 H S d ln K H ln K = + = RT R d(1/ T ) R ln KW 1/T H 0 /R H 0 = 51.0 kj mol -1 HCl H + + Cl - H2O H + + OH - [] [H + ] [OH - ]=10-14 M 2 25 OH - x OH - [OH - ]=x [H + ]
CAS NC(CH 2 ) 4 CN 1,4- C 6 H 8 N c.c d = Pa
1 200117 21512 CAS 111693 110 NC(CH 2 ) 4 CN 1,4- C 6 H 8 N 2 108.14 1 99 2-1. 2 1 295 159 c.c. 550 3 1.7-4.9 20 d 0.965 4 3.73 = 1 0.3 Pa 0.002 mmhg20 2 log Pow-0.32 m/z 41, 1.0 68 0.54 54 0.43 4 Koc9-16
untitled
er. 3.0SJ ver.3.0 SJ 3 2 1 ver.3.0 SJ 900 1200 () 400 2 ver.3.0 SJ OH OMe OMe O O O MeO O MeO OMe O O OMe O OMe OMe O O MeO O O H O O MeO OMe O H O OH OMe O CH 2 HO O O O O CH 3 OH HO OH OH HO OH O 1 3
DNA
1 1 1. 1 2. 2 3. 2 4. 2 2 1. 2 4 5 7 2. 7 3. DNA 8 4. 10 13 Plasmodium (P. falciparum) (P. vivax) (P. malariae) P. ovale 4 40% 200 7 40 48 72 1. - 1 - 2. DNA 3 3. 1) 2) 3) 4) 4. 1 1) - 2 - 2) EDTA 1) 1
MACS機器予算申請カタログ
MACS Instruments MACSQuant automacs MultiMACS gentlemacs MACSmix TM MACSQuant 3 MACSQuant Analyzer 10 MACSQuant VYBMACSQuant X MACSQuant X Coming soon! 384 well plate Laser Channel Filter (nm) Dye or Parameter
出展者マニュアル 01章 事務局からのお知らせ
Manual 2015 Exhibition 01-1 01-1-1 01-1-2 01-2 01-2-1 01-2-2 01-2-3 01-2-4 01-2-5 01-3 01-3-1 01-4 01-5 01-4-1 01-4-2 01-5-1 01-5-2 01 01-1-1 Exhibition Manual 2015 Exhibition Manual 2015 01-1-2.1 Exhibition
ネオマファーム施工標準軸組構造
注 1 注 2 注 2 SISI 1. 2. 30 35 40 50 15mm 303 (17kg/) 455 16mm, 18mm 250 (20kg/) 455 303 303 _ 6 455 650 10,465 910 2,730 1,820 1,820 910 2,275 1,365 415 450 650
-2-
-1- -2- -3- 1 2 Pseudomonas avenae 34 36 3 25 20 4 1 200 300 2 18 20 5 24 10 ml 20 12 24 3-4- 4 32 33 3 1 60 30 3cm 1 2 1 3 1 3 2 3 1 2 1 1 3 2 2 1 300 3 1 5 0 1 1 2 2 3 3 4-5- 1nl 2n2 3n3 4n4 4N 100 N
カタログ製造及び取り扱い
Aluminium Biphosphate, Solution 50 30kg AlHPO 2 4 3317.94 Aluminium Chloride, 6-Hydrate AlCl36H2O 241.43 Aluminium Metaphosphate AlPO3 3263.90 Aluminium Nitrate, 9-Hydrate AlNO3 3 9H2O 375.13 Aluminium
<4D F736F F D B BA908593B98AC782AB82E593E082CC88C091538AC7979D82C98AD682B782E992868AD495F18D908F912E646F63>
12 62 1800 14 3 11 14 4 14 4 30 ... 1... 1... 1... 2... 5... 6... 7... 10... 13... 18... 19 21 22 24 27 28 31 32 33... 34... 35... 37 2 1,650mm () H=2,500W=2,000 460m 750m 500m 42.1m 1.15m () 100% 80%
20 Cloning of the synthesizing enzyme gene of autoinducer regulating the biosynthesis of violacein
20 Cloning of the synthesizing enzyme gene of autoinducer regulating the biosynthesis of violacein 1115006 2009 3 3 4 1 6 6 7 12 13 2 14 14 14 18 20 3 22 22 22 24 27 28 29 30 Web 31 32 2 Pseudoalteromona
ブック 1.indb
20 29 29 18 21 29 10 30 31 10 11 12 30 13 10 30 14 11 30 15 12 16 13 17 14 18 15 19 16 20 17 21 18 10 20 29 82 83 84 85 86 87 88 20 10 89 20 12 11 90 20 13 12 91 20 14 13 92 20 14 14 93 15 15 94 15 16
17-05 THUNDERBIRD SYBR qpcr Mix (Code No. QPS-201, QPS-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4196K
17-05 THUNDERBIRD SYBR qpcr Mix (Code No. QPS-201, QPS-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4196K - 18 - [1] 1 [2] 2 [3] 3 [4] 5 [5] : RNA cdna 13 [6] 14 [7] 17 LightCycler TM Idaho
Tox
CytoTox 96 Non-Radioactive Cytotoxicity Assay No. TB163J 2000 8 G1780 I. 2 II. 2 III. A. 4 B. CytoTox 96 Assay 4 IV. A. 5 B. 5 C. LDH 5 V. A. 7 B. 8 C. LDH 8 D. 9 VI. A. 11 B. 12 VII. 13 VIII. 14 IX. A.
無印良品のスキンケア
2 3 4 5 P.22 P.10 P.18 P.14 P.24 Na 6 7 P.10 P.22 P.14 P.18 P.24 8 9 1701172 1,400 1701189 1,000 1081267 1,600 1701257 2,600 1125923 450 1081250 1,800 1125916 650 1081144 1,800 1081229 1,500 Na 1701240
1 2 http://www.japan-shop.jp/ 3 4 http://www.japan-shop.jp/ 5 6 http://www.japan-shop.jp/ 7 2,930mm 2,700 mm 2,950mm 2,930mm 2,950mm 2,700mm 2,930mm 2,950mm 2,700mm 8 http://www.japan-shop.jp/ 9 10 http://www.japan-shop.jp/
第18回海岸シンポジウム報告書
2011.6.25 2011.6.26 L1 2011.6.27 L2 2011.7.6 2011.12.7 2011.10-12 2011.9-10 2012.3.9 23 2012.4, 2013.8.30 2012.6.13 2013.9 2011.7-2011.12-2012.4 2011.12.27 2013.9 1m30 1 2 3 4 5 6 m 5.0m 2.0m -5.0m 1.0m