[PDF] 安心して機器をお使いいただくために AKTA

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1 ! One Point 1 20% % ml ÄKTAexplorer 10S, ÄKTAexplorer 10XT, ÄKTApurifier 10, ÄKTApurifier UPC 10 2µm 1 ml/min 0.35 MPa 2M NaOH ÄKTAexplorer, ÄKTApurifier ÄKTAexplorer 100, ÄKTApurifier 100, ÄKTApurifier UPC ÄKTA Sample Fiber UV Sample Fiber UV 10% Decon 90, Deconex 11, RBS M NaOH30% 2-23

2 ! UV UV-900 Check Lamp Intensity 210mm 85% 300nm 95% Check Lamp Run Time 200h ÄKTAexplorer, ÄKTApurifier Check Check Lamp Intensity 20% CheckCheck Lamp Run Time 4,000 2,000 4,000 2,000 Q A Check valve Q P-950 A Q A Q 280 nm254 nm A TEL Q ph A ph electrode, round tip, UPC Q UV UV A1 mau UV UV A2 UV-900 UV Q HiLoad A ÄKTA PEEK Union M6 female/1/16female SRTC SRTC-2 M6 female/m6 female Union 1/16female / M6 male Q HiPrep A ÄKTAexplorer 10S ÄKTApurifier HiPrep Back pressure 0.75 mmdelay Volume Q PC No System DiskWindows A USB 24

3 Q A P Q 5 Waste A 5 Q 1 ml/min 0.35 MPa A p.23 Q Frac-900Frac-920 A Q Superloop A1 O-ring O-ring A2 Superloop ÄKTAdesign UV User Club p.18 p.19 p.19 p.20 p.21 p.36 p.36 ÄKTAexplorer, ÄKTApurifier 25

4 ÄKTAFPLC ÄKTAFPLC PumpSeal 1 1 Injection Valve Accumulator Seal Frac FracTube Sensor Frac-900/920 1 UV Filter 1 UV Filter 1 Fraction UV P-920 Pump Seal FLOW FLOW Conductivity Cell Conductivity Cell PC Sample Pump Sample Pump Pump Switch valve A,B Fraction Collector ph 2 ph Aceton Frac Parameters Delay vol. UNICORN Fraction 1 Pump Seal 4 Pump Valve 2 Inlet Filter 4 Distributing Block INV Channel Plate INV On Line Filter Kit 1 26

5 ! One Point 1 2 Concentration 50%B 20ml/min ÄKTAFPLC UV UV 10% Decon 90, Deconex 11, RBS M NaOH30% 2-! Check Lamp Intensity R S 214.7mV Check Lamp Run Time Hg 2300h Zn 340H CheckCheck Lamp Intensity Hg RReference 150 mv280 nm CheckCheck Lamp Run Time Hg 280 nm 3,500 Zn Lamp Run Time 2,000 27

6 Q A Q ph A ph electrode, round tip, UPC ÄKTAFPLC Q UV UV A mau UV UV Q HiLoad A ÄKTA PEEK Union M6 female/1/16female SRTC SRTC-2 M6 female/m6 female Union 1/16female / M6 male Q PC No System DiskWindows A USB Q 5 Waste A 5 Q 1 ml/min 0.35 MPa A p.27 Q Frac-900Frac-920 A Q Superloop A1 O-ring O-ring A2 Superloop ÄKTAdesign UV User Club p.18 p.19 p.19 p.20 p.21 p.36 p.36 28

7 ÄKTAprime, ÄKTAprime plus 1 Piston cpl 3 Ballstopper & Seal 1 O-rings kit,p Glass Tube Gasket 3 CPU Injection Valve Gradient Valve Buffer Valve Mixer Fraction Collector Cond Monitor UV Monitor Pump FLOW FLOW Conductivity Cell Conductivity Cell ph UV Delay Vol. Fraction Drop Counter ÄKTAprime, ÄKTAprime plus! Check Lamp Intensity R S 214.7mV Check Lamp Run Time Hg 2300h Zn 340H CheckCheck Lamp Intensity Hg RReference 150 mv280 nm CheckCheck Lamp Run Time Hg 280 nm 3,500 29

8 ÄKTAprime, ÄKTAprime plus Q UV A UV 1 UV 1 M NaOH 1 ml / min 30 UV 96% System Wash method method Q A ÄKTAprime A System Wash method 28 Q A chart speed zero Rec on / off offup Q A UV 1 M NaOH Q A bdm/purge.asp Tel Upgrade Kit P-950,purge 50,000 Q A Q A Q 72 Change lamp or call service A UV UV 1 Q A Q no data A Q 62 Check that the tube position is OK A feed tube 30

9 Q Peak Integrate X A Peak Integrate X X Q RUN PrimeView A PC ÄKTAprime CheckOK Check communication. PC App. Connected! Check communication. Not Connected! PrimeView PC Q PrimeView Run Logbook A Properties Hide ViewWindow... Curves p.15? FAQ Q A Q ÄKTAprime UV A UV UV Set Parameters LampOn LampOff UV UV 1 UV ÄKTAprime, ÄKTAprime plus FAQ Q 1.5ml A 12 mm 175 Eppendorf tube holder Q PrimeView A 10 ManualRun 1 Q ÄKTAprime PrimeViewPC A PrimeView ÄKTAprime Q A PrimeViewPC PC ÄKTAprime PC ÄKTAprime RS-232C 1.8 m 5 m PC PC Tel Q ÄKTAprime REC-112 PrimeView A ÄKTAprime ver.2.01 PrimeView PrimeView PC PrimeView ver.2.01 ÄKTAprime Tel Q ÄKTAprime PC REC-112 A PC REC-112 ÄKTAprime ver.2.01 ver.2.01 PC ÄKTAprime Q 31

10 A ÄKTAprime Template Q ph A ph UV ph 88 µl PEEK 0.75 mm 10 cm 44.9 µl ph ph 4 2 M KNO3 =11 FAQ ÄKTAprime, ÄKTAprime plus Q A chart speed Q A 0.45 µm Q Template Result A Application Template Result AT Method Template MT Template Run Stored Method Result Application Template AT2003Aug1no1.RES Method Template MT2003Aug1no1.RES Run Stored Method 2003Aug1no1.RES Q PrimeView Evaluation Tile Tile A EditDelateChromatogram...Tile Q ÄKTAprime PC A PC ÄKTAprime 40 Copy Method PC p.12 PrimeView ÄKTAprime PC PC PrimeView PC Copy ÄKTAprime PC Method 40 PC PrimeView UVConductivity PC (LogBook) PC Integrate Excel Export Laptop PC p.33 ÄKTAprime 32

11 ÄKTAprime Yes Yes No Method Yes ÄKTAprime No ÄKTAprime plus No No ÄKTAprime No Yes Yes 50, PrimeView 5 upgrade WinXP/Laptop PRINTER FOR ÄKTA OA-Tap PrimeView 5 upgrade WinXP/Laptop PRINTER FOR ÄKTA OA-Tap Upgrade Kit P-950, purge ÄKTAprime Pump Yes Upgrade Kit P-950, purge No PrimeView 1 688,550 1 PrimeView 2 738,550 2 Method PC ÄKTAprime, ÄKTAprime plus ÄKTAdesign UV User Club p.18 p.19 p.19 p.20 p.36 p

12 ÄKTAxpress, ÄKTAcrossflow ÄKTAxpress ÄKTAxpress, ÄKTAcrossflow ÄKTAcrossflow ! One Point 2010 mm NaOH 20 34

13 1 In/Out 2 2 ÄKTAcrossflow In/Out System Control System Calibrate 2 In/Out 2 4 Monitor ZeroLS Start calibrate 5 Start calibrate 6 In/Out 2 ph 1 System Control System Caribrate 2 ph ph Measured Value Reference Value1 ph Read value1 3 ph ph Measured Value Reference Value2 ph Read value2 4 Close 35

14 User Club ÄKTA User Club Handbook Gel Filtration Principles and Methods HB09-02 Handbook Gel Filtration Principles and Methods Handbook Ion Exchange Chromatography & Chromatofocusing, Principles and Methods HB10-01 Handbook Ion Exchange Chromatography & Chromatofocusing, Principles and Methods Handbook Affinity Chromatography Principles and Methods HB08-01 Handbook Affinity Chromatography Principles and Methods Handbook Antibody Purification Handbook The Recombinant Protein Purification Handbook GST Gene Fusion System Handbook GST Gene Fusion System HB07-01 Handbook Protein Purification Protein PurificationPrinciples, High Resolution Methods, and Applications STREAMLINE Handbook Expanded Bed AdsorptionPrinciples and Methods Hydrophobic Interaction and Reversed Phase Chromatography, Principles and Methods Handbook Challenging Protein Purification HB13-01 Handbook HB05-02 Handbook HB06-03 Handbook HB12-02 User Club ÄKTA User Club ÄKTA PDF ÄKTA 1 /2 3 ÄKTA User Club ÄKTA 36

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