秋田応用微生物研究会
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2 13:00-13:10 13:10-13:30 13:30-13:50 (Tricholoma auratum) 13:50-14:10 14:10-14:30 14:30-14:50 14:50-15:10 α-msh 15:10-15:30 Lewis 15:30-15:50 15:50-16:10 16:10-16:30
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4 - (Tricholoma auratum) - ) ( () (tanning ) 1,2) MC3T3-E1 3) MC3T3-E1 ( ) [Tricholoma auratum] 4) MC3T3-E1 (ALP) Hoechst MC3T3-E1 ALP 20 T. auratum MeOH MC3T3-E1 ALP (22E, 24R)-ergosta-7,22-diene-3β,5α,6β-triol (1) µm MC3T3-E µm MC3T3-E1 1 ergosterol (2) ergocalciferol (3) ALP µm 1 MC3T3-E1 ALP µm 2 3 ALP 1 MC3T3-E1 5 6 MC3T3-E1 1 µm 1 MC3T3-E1 1 1) 2) 3) 1. Hata K. et al., Biol. Pharm. Bull., 23, (2000). 2. Hata K. et al., J. Nat. Prod., 65, (2002). 3. Mizutani K. et al., Biochem. Biophys. Res. Commun., 253, (1998). 4. Hata K. et al., Biol. Pharm. Bull., 25, (2002).
5 (Coprinus cinereus) 1 AmutBmut KF3#2 F1 40 RAPD Random amplified polymorphic DNA RFLP Restriction fragment length polymorphism Mapmaker AmutBmut pin1, eln6 KF3#2 F1 40 RAPD #2 DNA HindIII CHEF kb DNA trp1 BAC Bacterial Artificial Chromosome DH10B 215 RAPD 24 RFLP 13 RFLP CHEF 13 DNA 13 DNA pin1 eln6 pin1 V eln6 XIII BAC / 174 BAC PUKKILA, P. J., 1992 Methods of genetic manipulation in Coprinus cinereus, pp in Culture collection and breeding of edible mushrooms, edited by S. T. Chang, P. G. Miles and J. A. Buswell. Gordon and Breach, Philadelphia.
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7 α-msh α-melanocyte stimulating hormone(α-msh) B16-BL6 α-msh B16-BL6 Autocrine motility factor (AMF) α-msh B16-BL6 Western blot AMF receptor(gp78)α -MSH Flow cytometry α-msh B16-BL6 Transwell chamber α-msh AMF B16-BL6 AMF receptor (gp78) B16-BL6 α-msh Forskolin camp α-msh 3kDa B16-BL6 cycloheximide α-msh α-msh B16-BL6 B16-BL6 AMF receptor autocrine Murata, J., Ayukawa, K., Ogasawara, M., et al., Int. J. Cancer, 80, (1999). Murata, J., Ayukawa, K., Ogasawara, M., et al., Invasion Metastasis, 17, (1997).
8 Lewis (Helicobacter pylori) Lewis Lewis Lewis Lewis LPS AG polyc Slip on-off Lewis Lewis mimicry Yokota, S, Amano, K., Shibata, Y, et. Al. Infect. Immun. 68, , 2000 Yokota, S., Amano, K., Fujii, N., Yokochi, T. FEMS Microbiol. Lett. 185, , , , 2001.Amano, K. Trends Glycosci. Glycotech. 14, , 2002 Review
9 () - (DO) DO DO Spodoptera frugiperda (Sf-9) vgfpuv 1-L (310 5 cells/ml) 1.0 L/min (20 28) MOI=20 Sf DO 1.2 Fig. 1 Sf Sf-9 Monod (1) 0 28 DO (3 K S )0.16 ppm (Fig. 2) q = q max K S S O2 + S O2 (1) q max : K S : Sf-9 6 (MOI=0.1) DO 28 DO 4 (Fig. 3A B) GFPuv 28 (Fig. 3C) 28 0 DO Viable cell density [10 6 cells/ml] : 20 : 28 A DO [ppm] : 20 : 28 2 B Time post infection [dpi] [mol/10 6 cells/h] q DO [ppm] Fig. 1 Effect of DO on q of un infected (open key) and infected (closed key) Sf 9 cells., 34;, 28;, 22;, calculation. [mol/l] K S 2 K S = 1.69 mol/l Temperature [] Fig. 2 Effect of temperature on un infected Sf 9 cells. GFPuv fluorescence intensity : 20 : 28 K S of 100 C Fig. 3 Sequential changes in (A) viable cell density, (B) DO, and (C) GFPuv in media of vgfpuv infected Sf 9 cell cultures at 20 and 28
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秋田応用微生物研究会
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