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5 昭和45年9月 719 7

6 720 日本消化器病学会雑誌 (8) 第67巻第9号

7 昭和45年9月 721 (9)

8 722 日本消化器病学会雑誌 (10) 第67巻第9号

12 1) Gates, G.S. et al.: The cell population of human liver. J. Lab. clin. Med., 57: 182, ) Daoust, R. & Cantero, A.: The numerical (14)

proportions of cell types in rat liver during carcinogenesis by 4-dimethylaminoazobenzene (DAB)., Cancer Reseach 19: 757, 1959. 3) Grant, H.G. & Rees, KR.

13 proportions of cell types in rat liver during carcinogenesis by 4-dimethylaminoazobenzene (DAB)., Cancer Reseach 19: 757, ) Grant, H.G. & Rees, KR.: The precan cerousliver; correlations of histological and biochemical change in rats during prolonged administration of thioacetamide and "butter yellow"., Proc. Roy. Soc. B., 148: 177, ) Wiener. J. et al.: An electron microscopic study of reticuloendothelial system blockade., Amer. J. Path., 45: ) Brausteiner, H. et al.: Elektronmikroskopische Beobachtungen an normalen leberschnitten sowie nach Gallenstauung, Histamin und Allyformiatvergiftung., Z. ges. exper. Med., 121: 254, ) Cossel, L.: Beitrag zur Ultrastruktur der Blutgewebsgrenze in der Leber., Beitr. path. Anat., 120: 153, ) Horiuchi, T: Electron microscopic observa tionon the sinusoid of the normal rabbit liver., Kobe J Med. Sci., 16: ) Tanikawa, K et al.: Fine structure of the reticuloendothelial cells in the normal rat liver: morphological classification., Kurume Med. J., 12: 139, ) Ito, T. and Nemoto, Uber die Kuf ferschensternzellen und die "Fettspeicher ungszellen"("fat-storing cells") in der Blut kapillarenwandder menschlichen Leber., Fol., anat. jap., 24: 243, ) Yamagishi, M.: Electron microscope studies on the fine structure of the sinusoidal wall and fat-storing cells of rabbit livers., Arch., hist. jap., 18: 223, ) Novikoff, A.B. & Essner, S.: The liver cell (a review). Some new approach to its study., Amer. J. Med., 29: 102, ) Snack, H. et al.: Die Bindegewebszellen des Disseschen Raumes in der menschlichen Leber bei Normalfallen und pathologischen Zu standen.,wien. klin. Wschr., 78: 715, ) Safran, A.P. & Schaffner, F.: Chronic passive constitusion of the liver in man. Electron microscopic study of cell atrophy and intra lobularfibrosis., Amer. J. Path., 50: 447, ) Nakane, P.K.: Ito's fat-storing cell of the mouse liver., Anat. Rec., 145: 165, ) Nicolescu, P. & Rouiller, Cr.: Beziehungen zuwischen den Endothelzellen der Leber sinusoideund den von Kupfferschen Stern zellen.,elektronen mikroskopische Unter suchung.z. Zellforsch., 76: 313, ) Bronfenmajer, S. et al.: Fat-storing cells (lipocytes) in human liver., Arch. Path., 82: 447, ) Wood, E.M.: An ordered complex of fila mentssurrounding the lipid droplets in de veloppingadipose cells., Anat. Rec., 157: 437, ) Ito, T. & Shibasaki, S.: Electron microscopic study on the hepatic sinusoidal wall and the fat-storing cell in the normal human liver., Arch. histol. jap., 29: 137, ) Popper, H.: Distribution of vitamin A in tissue as visualized by fluorescence microscopy., Physio. Rev., 24: 205, ) Suzuki, Y.: Electron microscopic study on fat drop formation in the liver cell cytoplasm.,j. Electromicroscopy, 9: 24, ) Ashworth, C.T. et al.: Lipid absorption, transport and hepatic assimitation studied with electron microscopy., Am. J. Phys., 198(6): 1326, ) Caesar, R.: Elektronmikroskopischer Nach weisvon Fettpartikeln im Disseschen Raum., Zellforsch., 54: 793, ) Murray, R.G. & Freeman, S.: The mor phologicdistribution of intravenously injected fatty chyle and artifical fat emulsion in rats and dogs., J. Lab. clin. Med., 38: 56, ) Parks. H.F.: On the uptake of chylomicrons by hepatic cells of mice., Anat. Rec., 142: 320, ) Palays, S.L. & Karlin, L.J.: An electron microscopic study of the intestinal villies (II. The pathway of fat absorption)., J. Biophy. Biochem. Cytol., 5: 373, ) Shirotani, H.: Histochemical studies on fat metabolism by intravenous administration of fatty chyle., Arch. Jap. Chir., 26: 38, (15)

14 34) Stein, O. & Stein, Y.: The role of the liver in the metabolism of chylomicrons, studied by electron microscopic autoradiography., Lab. Invest., 17: 436, ) R.H. & Berman, S.L.: Relations be tweenkupffer cells and liver cells, functional studies., Arch. Path., 5: 1020, ) Haan, D.J.: Die Speicherung saurer Vital farbstoffein den Zellen mit beiziehung auf die Probelem der Phagozytose und der Zell gers. Arch., 201: 393, ) o, N. & o, A.G.: re Verteilung und Speichrungs-mechanismus der Beyer 205 (Germanin) in der Geweben., Acta Physiol. Hung., 3: 537, ) Fujino, S.: Experimental studies on the fat metabolism by the use of radioactive phos phorus.,arch. Jap. Chir., 28: 2668, ) Izukura, T.: Histochemical studies on intra venouslyadministered fat emulsion., Arch. Jap. Chir., 26: 215, ) Sasaki, H. et al.: Toxicity testing of fat emulsions. II. Ultrastructural changes in the liver following administration of a new intra venousfat emulsion (Intralipid)., Am. J. clin. Nutr., 16: 37, ) Nakamura, M.: Electron microscopic study on the metabolism of intravenously infused fat emulsion., Arch. Jap. Chir., 29: 299, 1960.

15 Fine Structure of Hepatic Reticuloendothelial System and Lipid Uptake Kohko Yoshimura Second Department of Internal Medicine, Kurume University School of Medicine, Fukuoka (Director: Prof. Kunio Okuda) Electron microscopic studies were made in rats to elucidate fine structure of the fat-storing cell and the role of the R.E.S, cells on hepatic uptake of lipid. 1) Fat-storing cell. The fat-storing cell of normal rats is clearly diffrenciated from the R.E.S. cells lining the sinusoid in its fine structure. It is about 10ƒÊ diameter, is located in the intercellular space between two hepatocytes or in the Disse space and contains several fat droplets of fairly uniform size in the cytoplasm. However, about 30% of the fat-storing cells have no fat droplets. In rats, in which a fat emulsion was injected intravenously for two weeks, the fat-storing cell with no fat droplet disappeared and the fat droplets in the cytoplasm were significantly increased in number. The reticulum fibers were frequently, observed in close proximity to the fat-storing cell. The lack of microvilli in its cell membrane and very few pinocytic invaginations suggest that this cell has no vigorous phagocytic activity as does the littoral cell. 2) Role of the R.E.S. on hepatic uptake of lipid. Thirty minutes to three hours after forced of cream, numerous electron dense particles of lipid, measuring about 0.1ƒÊ, in dia meter,-probably chylomicra-were seen in the Disse space and they appeared to be taken up into the hepatocyte by pinocytosis. However, very few or practically no lipid particles, were seen coming into the R.E.S. cell. On the other hand, when a fat emulsion was administered intravenously, lipid droplets about 0.5ƒÊ to 1.0ƒÊ in diameter, showed to be phagocytized into the Kupffer cells by the process of pinocytotic invagination of the cell membrane or engulfed by the cytoplasmic projections soon after the infusion. However, no lipid drops were seen to be taken up into the hepatocyte at this time. Thirty minutes to two hours after the infusion, numerous small dense particles of lipids, about 0.1ƒÊ in diameter, were seen in the Disse space, being pinocytosed into the hepatocytes. They seemed to be liberated from the Kupffer cells in which fat drops have been picked up. When a fat emulsion was administered intravenously under the R.E.S. blockade, uptake of lipid into the Kupffer cell and the subsequent accumula tionof lipid in the hepatocyte were delayed. In the uptake of lipid by the hepatocyte, certain physicochemical propeties of the fat droplets may be the determining factors, and the R.E.S. seems to play a role in the disposal of intravenously introduced fat, probably conditioning it in such a way as to enable the hepatocyte to phagocytiz it in its altered form. (17)

STUDIES ON THE RELATION BETWEEN LATE DUMPING SYNDROME AND GLUCAGON RESPONCES TO GLUCOSE Taisuke MATSUI The First Department of Surgery, Nara Medical U

STUDIES ON THE RELATION BETWEEN LATE DUMPING SYNDROME AND GLUCAGON RESPONCES TO GLUCOSE Taisuke MATSUI The First Department of Surgery, Nara Medical University, Kashihara (Director: Prof. H. Nakano) This