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3 PHZ投 状 赤 血 球 数(reticulocyte 与 ア カ タ ラ セ ミア マ ウ ス の 血 中 カ タ ラ ゼ count)を 算 出 し,総 赤 血 球 数 と綱 状 赤 血 球 数 の 差 を 成 熟 赤 血 球 数 (erythrocyte 4) count)と 状 赤 血 球 数 算 出 傾(104個/ 3) a:成 熟 赤 血 球107個 当 りの カ タ ラーゼ 活 性 値(PU/107個) b:網 を用 い,シ ア ン メ ト ヘ モグ ロ ビン法 でヘ モ グロ ビン濃度 を求め た. 5) y:網 し た. ヘ モグ ロ ビン濃度 の測 定 Van kampen-zijilstra液 状 赤 血 球107個 当 りの カ タ ラーゼ 活 性 値(PU/107個) 網 状 お よ び 成 熟 赤 血 球 数 当 りの カ タ ラ ー ゼ 成 熟 お よ び網 状 赤 血 球 数 算 出値 と血 中 力 タ ラ ー ゼ 活 性 測 定 値 を用 い て 回 帰 直 線(2)を求 め,網 活性値 の 算出 状 お よ び 成 熟 赤 血 球 の 細 胞 数 当 りの カ タラ ー ゼ 単 位 血 液 量 当 りの カ タ ラー ゼ 活 性 は,溶 血 液 と して 測 定 す るの で,成 活 性 を算 出 し た. 熟 赤 血 球 カ タ ラー ゼ 活 実 性 と網 状 赤 血 球 カ タ ラ ー ゼ 活 性 の 和 と し て,次 の 式 で 表 し た. 1. z=ax+by (1) z/x=a+b y/x 血 中 の,ヘ 験 結 果 モ グ ロ ビ ン 濃 度,総 網 状 赤 血 球 含 有 比,網 (2) 赤 血 球 数, 状赤 血球数 お よび成 熟 赤 血球 数 の 算 出 値 の 経 日変 動 の 関 係 ただし Fig ) ヘ モ グ ロ ビ ン濃 度,総 赤 血 球 数,網 状赤 z:血 中 カ タ ラ ー ゼ 活 性 値(PU/ml) 血 球 含 有 比(網 状 赤 血 球 数/総 赤 血 球 数 比),網 x:成 熟 赤 血 球 数 算 出 値(104個/吋) 状 赤 血 球 数 お よ び 成 熟 赤 血 球 数 の 算 出値 に つ い 1 Chages red and treated in hemoglobin cells) ratio catalase with concentration ( - ), activity phenylhydrazine ( œ- œ), reticulocyte ( - ) (c) in the red cell count (š --š ) blood of Arrows;phenylhydrazine count normal ( - ) and mice injection (a), erythrocyte (A) and (reticulocytes/total count acatalasemic ( -- ) (b), mice (B)

4 Table 1 Lag correlation coefficients (ra) between three kinds of cell counts (reticulocyte count, eryth rocyte count and red cell count) and catalase activity in the blood of normal and acatalasemic mice k; lag correlation order, rc; lag correlation coefficient, **; null hypothesis of the popula tion coefficients p=0 is rejected within 1 % error. N; normal mice, A.; acatalasemic mice

5 Table 2 Catalase activities in erythrocytes and reticulocytes in the blood of normal and acatalasemic mice (Inducing stage) N.: normal mice, A.: acatalasemic mice Fig. 2 Catalase activity in reticulocytes (R) and erythrocytes (E) expressed as per cell (A) and per hemoglobin (B) in the blood of normal (Nor.) and acatalasemic (Act.) mice

6 Table 3 Catalase activities in erythrocytes and reticulocytes in the blood of normal and acatalasemic mice in inducing and recovery stage N.: normal mice, A.: acatalasemic mice, I: inducing stage, R: recovery stage, T: total Fig. 3 Chages in estimated catalase activity in the blood (PU/ml) in reticulocytes ( ) and erythrocytes (---) from inducing and recovery stages separate ly, total of reticulocytes and eryth rocytes( œ- œ), and actural blood catalase activity ( - ) of normal (A) and acatalasemic (B) mice

7

8 1) Takahara S: Progressive oral gangrene probably due to lack of catalase in the blood. Lancet (1952) 2, ) Ogata M, Sadamoto M and Takahara S: On the minimal catalatic activity in Japanese acatalasemic blood. Proc Jpn Acad (1966) 42, ) Ogata M, Tomokuni K and Takahara S: Catalatic activity of immature and mature red blood cells in Japanese acatalasemia. Acta Med Okayama (1969) 23, ) Ogata M and Satoh Y: Isoelectric focusing of catalase from acatalasemic mouse and human blood, and cultured human skin fibroblast, Electrophoresis (1988) 9, ) Ogata M, Suzuki K and Satoh Y: Characterization of human residual catalase of an acatalasemic patient by isoelectric focusing and sodium dodesyl sulfate-polyacrylamide gel electrophoresis foll owed by electrophoretic blotting and immunodetection. Electrophoresis (1989) 10, ) Ogata M, Tomokuni K, Watanabe S, Osaki H, Sadamoto N and Takahara S: Residual catalase in the blood of Japanese acatalasemia. Tohoku J Exp Med (1972) 107, ) Aebi H, Heiriger JP, Butler R and Hassig H: Two cases of acatalasemia in Switzerland. Experimentia (1961) 17, ) Aebi H and Cantz M: Uber die cellulare Verteitung der Katalase im Blut homozygoter and heterozygoter Defecttrager (Akatalasie). Humangenetik (1966) 3, ) Feinstein RN, Seaholm JE, Howard JB and Russell WL: Acatalasemic mice. Proc Natl Acad Sci Wasc (1964) 52, ) Ogata M, Inoue T, Tomokuni K and Takahara S: Catalase activity of immature and mature red cells from acatalasernic mouse mutant. Acta Heamatol (1970) 44, ) Feinstein RN: Perborate as substrate in a new assay of catalase. J Biol Chem (1949) 180, ) Feinstein RN, Howard JB and Savol R: Heat and Urea stability of blood catalase of catalase mutant mouse strains. Experimentia (1971) 27,

9 15) Ogata M, Fujii T and Takahara S: Properties of catalase protein in immature and mature red cells of acatalasemic and hypocatalasemic mouse mutants. Acta Med Okayama (1971) 25,

10 Catalase activity in the blood of acatalasemic mice treated with phenylhydrazine Masako IDEN Department of Public Health, Okayama University Medical School, Okayama 700, Japan (Director: Prof. M. Ogata) To clarify the enzymatic properties of catalase from the acatalasemic the enzymatic activity during maturation mice, the change in of reticulocytes was observed both in normal and anemic conditions. Catalase activity in the blood of acatalasemic mice retained its maximum level 5 days after final injection of phenylhydrazine. An intimate lag correlation coefficient was obtained between reticulocyte count and catalase activity in blood by one day shift in acatalasemic mice and three days shift in normal mice, which corresponded to the interval between maximum peaks of reticulocyte count and catalase activity. The erythrocytes and reticulocytes were also counted, and the catalase activity per eryth rocyte and reticulocyte in the blood of normal and acatalasemic mice was calculated. The catalase activity in blood due to reticulocyte retained its maximum level 5 days after injection of phenylhydrazine in normal and acatalasemic mice. The results revealed that catalase activity in acatalasemic mice was very low in reticulocyte and yet its degeneration by maturation was considerably great. Catalase activity per reticulocyte in the blood of acatalasemic mice was 55% of that of normal mice. Degeneration ratio expressed as (catalase activity in reticulocytes-that in erythrocytes)/catalase activity in reticulocytes was 88% in acatalasemic mice and 34% in normal mice. The difference in catalase activity between reticulocytes and erythrocytes blood of acatalasemic activity in erythrocytes in the mice was also higher than that of normal mice. Thus the catalase in the blood of acatalasemic mice was 10% of that of normal mice.

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