明海大学歯学雑誌 44‐2☆/学術大会抄録(44‐2)26
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1 26 S : QOL Fujifilm 3 Fujifilm 13 W 7 D 10 H cm FinePix REAL 3D w3m, Fujifilm 3D, Armonicos Epipolar RGB Correction using Color Information, CCI BRGBmax RGBmin2 Y R G B CCI RGB stereo lithography STL Poisson surface reconstruction
2 S Crystaleye Spectrophotometer CIELAB genie SultanHealthcare elite stone Zhermach 9 IP67 ABS 3 SPSS Ver.20.0 IBM 9 A SD CV CV L* R 0.456, a* R 0.639, b* R 0.594, c* R p 0.01 a* b* Magnolol honokiol Porphyromonas gingivalis TNF-α Porphyromonas gingivalis LPS TNF-α LPS nuclear factor kappa-b NF-κB Magnolol honokiol eugenol eugenol Murakami bis-eugenol eugenol Escherichia coli LPS magnolol honokiol P. gingivalis LPS RAW264.7 P. gingivalis LPS TNF-α magnolol honokiol 1Magnolol, honokiol, eugenol bis-eugenol eugenol 2 RAW cell counting kit-8 3 LPS Porphyromonas gingivalis ATCC LPS TNF-α TNF-α 4 TNF-α mrna TaqMan probe/primer mixture PCR GeneAmp SDS 5700 TNF-α ELISA kit 5 COX-2 mrna PCR
3 26 S25 6 NF-κB IκB-α IκB-α IκB-α Western blot NF-κB NF-κB ELISA-based assay kit LPS RAW264.7 TNF-α μm LPS TNF-α magnolol, honokiol 50 μm RAW264.7 LPS TNF-α eugenol, bis-eugenol Magnolol honokiol TNF-α COX-2 NF-κB magnolol honokiol IκB-α NF-κB p65, p50, p52 NF-κB Magnolol honokiol NF-κB TNF-α CT CBCT A CBCT CBCT 3 Position 1 3 CT Veraview epoces 3 Df FOV field of view 40 mm 40 mm i-view CBCT Student s t-test SPSS 1 CBCT 6 n 0.1 mm 2 CBCT Position Position Position Position 1 Position 2 p 0.01 Position P P Position 1 Position 2, Position 1 Position 3 p 0.05 Position 2 Position 3
4 S A-PD B- PD 2 1 Kennedy A-PD B-PD 1 3 Okutsu g t 5 1 A-PD B-PD A-PD N, B- PD N 1 A-PD N, B-PD N 3 A-PD B-PD A-PD B-PD A-PD B-PD A-PD B-PD A-PD B-PD A-PD B-PD CBCT CT CBCT Hellman
5 26 S27 Hellman AA Hellman CBCT 90 kv 4mA 33 3 VPA PLUS A A A A 1 C 1 A A B C CBCT Veraview epocs 60, 70, 80 kv 8, 16, 32, 48, 64, 96, 160 mas Fujifilm ST-VI PTW Diamentor E2 Fujifilm Computed Radiography CRFCR XL-1 DICOM PC ROI CR EIZO FlexScan EV 2116 W 5 70 kv mas mas 70 kv 8 mas 160 mas 20
6 S kv 70 mas 4.8 cgycm cgycm cgycm 2! Sprague-Dawley 6 EKG EMG AD MA Cz-P 3/P4 EEGEOG 10 mg/kg :00 7 D712 D1216 D16 EMG, EOG, EEG, EKG 4 epochs 200 μspulse, 0.2 Hz 5 3 THTH latency, duration, AUC 5 3 QWB1-3 QS1-3 QWA1-3 TH QWB1 QS1 TH D7 TH D12 TH D EEG D7 δ δ, θ, α, β D16 δ D7 δ D Mangostin Mangosteen, scientific name Garcinia mangostana, is a tree found in South East Asia, and pericarps of the fruit have been used in folk medicine for the treatment of many
7 26 S29 human illnesses such as skin and wound infections, and inflammatory diseases. Xanthone extracts from G. mangostana have been reported with chemoprevention effects against the chemically induced colon cancer via the reduction of c-myc expression, suppression of tumor growth and metastasis in a mouse model of mammary cancer, and a recent report showed the inhibition of prostate cancer growth by α - mangostin, the main constituent of the G. mangostana xanthones. However, it is unclear whether α -mangostin induces cell death to oral cancer. Then, this study examined the impact of α -mangostin against human oral squamous cell carcinoma (HOSCC). At first, we analyzed the expression of c- myc in 5 HOSCC cells. The expression level of c-myc mrna was maximum in SAS cells, whereas, it was minimum in HSC-4 cells. Hence, SAS cells were treated with α -mangostin. The α -mangostin slightly induced cell death to SAS cells, but not strongly. Synergistic effects by the combined treatment of α -mangostin and anti-cancer drugs were reported. Then, we attempted to evaluate the synergistic effect on cell growth when cytokine, TNF related apoptosis inducing Ligand (TRAIL), was used with α -mangostin. It was demonstrated that the combined treatment of α - mangostin and TRAIL in SAS cells led to apoptosis via the activation of caspases-9, -3/ 7. Furthermore, this apoptosis was induced by G1 cell cycle arrest in SAS cells. These data suggested that the combination therapy by α -mangostin and TRAIL might be a great useful with a tremendous amount of potential as an anti-oral cancer drug. keratoepithelin keratoepithelin kera RGD M-CSF monocyte/macrophage kera TGF-β1-inducible gene-h 3 BIGH3 mrna fluorescent differential display 1RANKL BIGH3 TGF-β RANKL TGF-β 2 BIGH3 TRAP 3 BIGH3 RGD RANKL 4 α Vβ 3 β 3 RGD kera RGD kera BIGH3 mrna RANKL RGD mrna RANKL kera kera RANKL kera kera Kera
8 S kera a-pdt a-pdt in vitro a-pdt a-pdt in vitro mm 16 mm, SLA TSVB16, Zimmer Dental, USA Aggregatibacter actinomycetemcomitans ATCC A. a. 2 1 Control 2 MB0.01 Ondine Biopharma Corp, Canada nm 220 mw PeriowaveTM, Periowave Dental Technologies, Canada 1 4 a-pdt MB A. a MC3T3-E1RIKEN Cell Bank CellTiter-Glo Luminescent Cell Viability Assay Promega, USA5 MB a-pdt 1 A. a MB a-pdt 2 MB a-pdt 3 MB a-pdt MB a-pdt MB a- PDT MB a-pdt a- PDT MB KRS-10-2 KRS-10F-2 CA-R,Y,B,P1,P2,B1,B2
9 26 S31 40 g 4g 0.4 g VM , 1, 2 cm KRS KRS-10 F B 3030 CM-600 d, KONICA MINOLTA KRS-10-2 KRS- 10 F-2 CA-Y 0.5 cm 1.91 CA-B1 1 cm 0.26 CA-R, Y, B CA-Y KRS cm 2cm CA-P1, P2, B1, B2 CA-B1 0.5 cm 1cm 1.61 KRS-10-2 KRS-10F-2 CA-R,Y,B 0.5 cm 1, 2 cm CA-P1, P2, B1, B American Association for Cancer Research : AACR CSCs CD44, Aldehyde dehydrogenase ALDHABCG2 CD44 HSC-2, HSC-3 epidermal growth factor receptor ; EGFR EGFR CD44 CD EGFR EGFR OH
10 S negative Porphyromonas gingivalis LPS 2 LPS 1 Eugenol p-cresol, p-hydroxyanisole pha magnolol, honokiol resveratrol 4-allylphenol Parkway ScientificorcinolEugenol p-cresol pha 2 RAW Porphyromonas gingivalis ATCC Yoshimura LPS Escherichia coli O 111 B 4 LPS LBL 3 Real-time PCR COX-2 COX-2 Western blot TNF-α ELISA kit R&D systems 4 NF-κB ELISA kit Activemotif NF-κB κb IκB-α IκB-α IκB-α Ser 32 Western blot p-cresol pha RAW E. coli LPS NF-κB COX-2 ortho-bisphenol magnolol, honokiol P. g. LPS NF-κB COX-2 TNF-α polyphenol resveratrol P. g NF-κB COX-2 4-allylphenol, orcinol primary T CXCL9, CXCL10, CXCL11 CXCR3 CXCR3 1 T Th1 T CXCL9, CXCL10, CXCL 11 SCCVII SCVII CXCL9, CXCL10, CXCL11 SCCVII blastcidin ELISA negative control
11 26 S33 CXCL9, CXCL10, CXCL11 CXCL9 387 pg/ml /10 5 cells, CXCL pg/ml /10 5 cells,cxcl1137pg/ml /10 5 cells in vitro C3H/HeJ htert HGEP Ca9-22, HSC-2, HSC-3, HSC-4HGEP TNF-α IL-1β IFN-γ NaB Ca9-22 HSC-4 HSC-3, HSC-4 IL-1β,IFN-γ,NaB α 5 sirna htert HSC-3, HSC-4 Ca9-22 dysplasia-carcinoma sequence mm GS 7
12 S cm cm cm cm 3 P cm cm cm cm P 0.05 QOL 3 A B C 3 D E F Saita STAI Stait-Trait Anxiety Iventory O-HIP14 Oral Health Impact Profile QOL VAS A C STAI O-HIP14 B VAS A C B Mineral Trioxide Aggregate MTA Mineral Trioxide Aggregate
13 26 S35 MTA MTA MTA MTA DSPP, Wnt 15 6 SD MTA 7, 14, 21 5 MTA 7, 14, 28 EDTA HE DSPP, Wnt MTA 14, 21 MTA Dentin bridge Wnt MTA MTA MTA Wnt MTA QLF mm 2.2 mm CaCl2, 2.2 mm NaH2PO4, 50mM ph , 50, 100, 200 μm 0.9 mm CaCl2 2 H2O, 3 mm KH2PO4, 130 mm KCl, 20 mm HEPES, 0.68 mm 10 ppmf, ph QLF QLF-D Biluminator TM 2, Inspektor Research Systems ΔQ Px ΔQ Student t 50 μm ΔQ 100 μm 200 μm ΔQ
14 S X X CCD ADX 4000 IP CCD PC PC E/F InSight 1/3 1/ m 1mSv 2m
gtf Wnt5a-Ror2 IL-8 in vitro Ozony Tron P. gingivalis SOD ELISA Location of main occluding areas and masticatory ability in patients with reduced occlusal support 12-Methacryloyloxydodecylpyridinium bromide
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