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1 pp. ATP ε ATP ATP ATP ε Bacillus PS ATP ATP H H µ H ATP ATP ADP µ H ATP FoF -ATP FoF -ATP µ H H ADP ATP H Na On the regulatory role of the epsilon subunit in ATP synthase Yasuyuki Kato-Yamada Department of Life Science, College of Science, Rikkyo University,, Nishi- Ikebukuro, Toshima-ku, Tokyo, Japan FoF -ATP µ H H ADP ATP FoF H Fo c Fo ATP a b c H Fo F b α β γ δ ε α β γδε β ATP Fo FMg F ATP ATP F - ATPase FoF -ATP ATP H ATP µ H F -ATPase FoF
2 ATP a FoF -ATP b F -ATPase c Fo ATP FoF ATP ATP µ H John Walker F -ATPase X α β γ α β α β γ β ATP ADP γ α β Paul Boyer γ γ γ γ γεc ATP ATP γε F H c Fo F -ATPase γ ATP β γ ATP ATP ADP ATP ADP ATP ADP α ATP ATP ADP µ H ATP
3 ADP ATP ATP ATP ATP ATP γ a ATP ATP ATPase ph ATP b ATP ADP ε ε ε β N α C F Fo F F δ F ε Fo F ATP α β γ F F ATP a ATP γ b ATP ATPase F -ATPase ε a F -ATPase ε b F -ATPase ε
4 α β γ F ATPase ε FoF F -ATPase ATPase F -ATPase ε ε afof F -ATPase ATP ε ATP ε ε ATP F -ATPase ε Bacillus PS F -ATPase TF ε TF F -ATPase TF α β γ δ ε ATPase ε α β γ α β γε ATP µm µm ATP NADH ATP NADH nm ATP ATPase ε α β γ α β γ ADP ε α β γε ATP µm a ε ε ATP ε b TF ε ATP ATPase F -ATPase ε ε F -ATPase ε F -ATPase ε F -ATPase a F -ATPase ε F -ATPase ε ε ATP F - ATPase HPLC SDS-PAGE F -ATPase ε F -ATPase ε ATP TF ATPase ATP ε
5 F -ATPase ε b TFoF -ATP ATP ε F -ATPase ε ATP FoF ε ε ε F -ATPase ε ATP ε ATPase ATP ε ATPase µ H ε ε γ ATP ATP ε ε FoF ATP ATP Bacillus PS F -ATPase ATP ε ATPase ε ATP ATP ε ATP ATPase ATP ε Fo F ATP H FoF F -ATPase ε C α ATP ε C ATP F -ATPase ATP ε ATP ATP ε ε ATP ε ε Py- MOL http: PDB ID E FS ε ATP Bacillus PS ATP ε ε ATP ε C ATP ε ATP
6 HPLC UTP CTP GTP ADP ε ATP ε X ATP ε a ε - b ATP ε ε ATP HPLC ATP ε ε ATP Kd µm ADP µm GDP mm ATP ε ATP ATP Kd µm ATP ATP ATP ATP mm ATP Kd mm ATP HPLC F -ATPase ε ATP Kd µm ATP ε ATP Bacillus Bacillus subtilis ATP ε ATP Kd mm ATP ε ATP ε ATP ATP ε ATP ε a N C ATP PDB ID E Y b ATP ε
7 ε ATP ATPase RA a ε ATP b ATPase ε ε ATP ATP ATP ε C ATP ε HPLC ATP ATP F -ATPase ATPase RA ATP ATPase R ATPase RA ATP ATP ε ATP ε ATP ε ATP ATP ATP ATPase ATP
8 µ H ATP µ H ATP ε ATPase ATP ATP ATP µ H ε ε ATP ε ε ATP Bacillus ε ATP ATP ε KdmM ATP ATP ε ATP ε ATP ε ATP ε ATP ATP ATP ε CFP YFP ATP ATP ATP ATP ATP ATP ATP ATP ε Berg, J.M., Tymoczko, J.L., & Stryer, L.Biochemistry, th Ed., W.H. Freeman and Co., New York. Boyer, P.D.Annu. Rev. Biochem.,,. Kinosita, K., Jr., Yasuda, R., & Noji, H.Essays Biochem.,,. Yoshida, M., Muneyuki, E., & Hisabori, T.Nat. Rev. Mol. Cell Biol.,,. Senior, A.E., Nadanaciva, S., & Weber, J.Biochim. Biophys. Acta,,. Abrahams, J.P., Leslie, A., Lutter, R., & Walker, J.E. Nature,,. Noji, H., Yasuda, R., Yoshida, M., & Kinosita, K., Jr. Nature,,. Kato-Yamada, Y., Noji, H., Yasuda, R., Kinosita, K., Jr., & Yoshida, M.J. Biol. Chem.,,. Sambongi, Y., Iko, Y., Tanabe, M., Omote, H., Iwamoto- Kihara, A., Ueda, I., Yanagida, T., Wada, Y., & Futai, M. Science,,. Tsunoda, S.P., Aggeler, R., Yoshida, M., & Capaldi, R.A. Proc. Natl. Acad. Sci., U.S.A.,,. Yasuda, R., Noji, H., Kinosita, K., Jr., & Yoshida, M. Cell,,. Yasuda, R., Noji, H., Yoshida, M., Kinosita, K., Jr., & Itoh, H. Nature,,. Nishizaka, T., Oiwa, K., Noji, H., Kimura, S., Muneyuki, E., Yoshida, M., & Kinosita, K., Jr.Nat. Struct. Mol. Biol.,,. Ariga, T., Muneyuki, E., & Yoshida, M.Nat. Struct. Mol. Biol.,,. Adachi, K., Oiwa, K., Nishizaka, T., Furuike, S., Noji, H., Itoh, H., Yoshida, M., & Kinosita, K., Jr.Cell,,. Vasilyeva, E.A., Minkov, I.B., Fitin, A.F., & Vinogradov, A.D. Biochem. J.,,. Jault, J.-M. & Allison, W.S. J. Biol. Chem.,,. Matsui, T., Muneyuki, E., Honda, M., Allison, W.S., Dou, C., & Yoshida, M.J. Biol. Chem.,,.
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Kazumitsu Ueda, [Department of Applied Life Science, Kyoto University Graduate School of Agriculture, Kitashirakawa, Sakyo-ku, Kyoto 606-01, Japan] Molecular Mechanisms for Drug Transport by MDR1/P-glycoprotein
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Various function and toxicity of iron Key words 1) Hentze, M. W. et al: Cell, 117, 285-297 (2004) 2) Ganz, T.: Blood, 102, 783-788 (2003) 3) Nemeth, E. et al.: Science, 306, 2090-2093 (2004) 4) Hentze,
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4) H. Takayama et al.: J. Med. Chem., 45, 1949 (2002). 5) H. Takayama et al.: J. Am. Chem. Soc., 112, 8635 (2000). 6) H. Takayama et al.: Tetrahedron Lett., 42, 2995 (2001). 9) M. Kitajima et al: Chem.
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