Key words: Limulus amoebocyte lysate assay, Limulus lysate micro-technique procedure, Chromogenic substrate assay, Rapid detection of bacteriuria
|
|
- けいしょう きちや
- 4 years ago
- Views:
Transcription
1 Key words: Limulus amoebocyte lysate assay, Limulus lysate micro-technique procedure, Chromogenic substrate assay, Rapid detection of bacteriuria
2 Fig. 1 Preparation of chromogenic substrate LAL assay on urine specimens
3 Fig. 2 Standard curve of LAL assay by using E. coli (O111-B4) standard endotoxin Each point represents the means }the standard deviation. Table 1 Results of quantitative urine culture (228 samples) ND.*; not detected(<2 ~103CFU/ml)
4 Fig. 3 Comparison of the results of LAL assay on urine specimens including E. coli (ATCC 25922) according to various dilution rates and incubation times LAL assay was performed on undiluted urine specimens (N) and diluted to 1: 10 ( ), 1: 100 ( œ) and 1: 1000 ( ) with endotoxin free water. Fig. 4 Results of the absorbance values in LAL assay on diluted 58urine specimens (ND group)
5 Fig. 5 Results of the absorbance assay on diluted urine specimens group) values in LAL (GPC, YLO Fig. 6 Results of the absorbance values in LAL assay on diluted urine specimens (GNB group) Fig. 7 Comparison of the absorbance values in LAL assay on specimens of four urine groups (GNB, GPC, YLO and ND groups) (): number of urine isolated 105CFU/ml of bacteria p values compared GNB group with each GPC,YLO and ND group
6 Table 2 Comparison of the absorbance values in LAL assay between GNB group and other 3 groups Table 3 Correlation of the results of LAL assay with four groups a): GNB, GPC and YLO were the groups of a): GNB, Gram-negative bacteria ( 105CFU/ml) GPB, Gram-positive cocci ( 105CFU/ml) YLO, Yeast-like organism( 105CFU/ml) ND, Not detected(<2 ~103CFU/ml) b): N, Number of urine specimens c): Av, Average of absorbance values; SD, Standard deviation d): p values compared GNB group with each GPC, YLO and ND group urine contained 105 CFU/ml of each kind of bacteria in urine. ND was the group of urine not detected by the quantitative urine culture (<2 ~103CFU/ml) b): above 0.15 with OD values at 410nm Table 4 Results of LAL assay on urine specimens compared with quantitative culture a): Total No. of GPC, YLO and ND group
7
8 Detection of endotoxin in antibiotic solutions with limulus amobocyte lysate. Antimicrob. Agents Chemother., 23: , ) Iwanaga, S., Morita, T., Harada, T., Naka- mura, S., Kimura, T. & Sakakibara, S.: Chromogenic substrates for horseshoe crab clotting enzyme. Its application for the assay of bacterial endotoxins. Haemostatis.. 7: : , , ) Levin, J. & Bang, F. B.: The role of endotoxin in the extracellular coagulation of limulus blood. Bull. Johns Hopkins Hosp., 115: 265 2) Siegel, S. E. & Nachum, R.: Use of the limulus lysate assay (LAL) for the detection and quantitation of endotoxin. In Prespective in toxinology (Bernheimer, A. W. ed.) p.72-81, John Willy & Sons, New York, ) Case, M. J., Ryther, S. S., Novitsky, T. J.: 6) Jorgensen, J. H. & Jones, P. M.: Comparative evaluation of the limulus assay and the direct Gram stain for detection of significant bacteriuria,. Am. J. CLin. Pathol., 63: , ) Jorgensen, J. H., Carvajal, H. F., Chipps, B. E. & Smith, R. F.: Rapid detection of Gramnegative bacteriuria by use of the limuls endotoxin assay. Appl. Microbiol., 26: 38-42, ) Jorgensen, J. H. & Alexander, G. A.: Rapid ditection of significant bacteriuria by use of an automated limulus amoebocyte lysate assay. J. Clin. microbiol., 16: , ) Nachum, R. & Shanbrom, E.: Rapid detection of Gram-negative bacteriuria by limulus amoebocyte lysate assay. J. Clin. Microbiol., 10) Nachum, R. & Berzofsky, R. N.: Chromogenic limulus amoebocyte assay for rapid detection of Gram-negative bacteriuria. J. Clin. Microbiol., 21: , ) Prior, R. B. & Spagna, V. A.: Rapid evaluation of gonococcal and nongonococcal urethritis in men with limulus amoebocyte lysate and a chromogenic substrate. J. Clin. Microbiol., 17: 12) Kakinuma, A., Asano, T., Torii, H. & Sugino, Y.: Gelation of limulus amoebocyte lysate by an antitumor (1 3)-ƒÀ-D-glucan. Biochem. Biophys. Res. Communs., 101: , ) Obayashi, T., Tamura, H., Tanaka, S., Ohki, M., Takahashi, S., Arai, M., Masuda, M. & Kawai, T.: A new endotoxin-specific assay using recombined limulus coagulation enzymes and its clinical aplications. Clinical Chimica Acta, 149: 55-65, ) Friedman, R. B., Kwean, H. C. & Szczecinski, M.: Imporved sensitivity of chromogenic substrate assays for urokinase and plasmin. Thrombosis Reserch, 12: 37-46, ) Kass, E. H.: Asymptomatic infections of the
9 urinary tract. Trans. Assoc. Am. Physicians, 69: 56-64, ) Prior, R. B. & Spagna, V. A.: Application of a limulus test device in rapid evaluation of gonoconcal and nongonococcal urethritis in males. J. Clin. Microbiol., 14: , Evaluation of Screening of Bacteriuria -Rapid Detection of Gram-Negative Bacteriuria by Using the chromogenic Limulus Lysate Microassay- Takahiro NARITA, Kazuyuki SUGAHARA, Keizo YAMAGUCHI & Toshiaki USUI Department of Clinical Laboratory, School of Medicine Nagasaki University Hospital The use of Limulus Amoebocyte Lysate (LAL) assay in determining bacteriuria due to gramnegative organisms had been studied extensively. Many authors and workers have published papers concerning this study. And Iwanaga et al., in 1978, introduced the chromogenic substrate LAL assay. In this study, bacteriuria due to gram-negative bacteria (GNB) was detected by using chromogenic substrate LAL assay. On the basis of this method, micro-technique procedure was applied using small amount of urine specimens and solution composed of LAL and chromogenic substrate. We used 100- fold diluted urine on LAL assay, mixed and incubated at 37 Ž for 30 minutes and examined macroscopically by using spectrophotometer (microplate reader). Out of 228 urine samples tested, 31 out of the 32 samples (96.9%) were positive which correlated well with urine samples which containing more than 105 CFU/ml of gram-negative bacteria. Out of 8 urine samples which contained more than 104 CFU/ml up to 105 CFU/ml of GNB, 6 (75.0%) were positive. Out of 11 urine samples which contained less than 104 of GNB, 4 (36.4%) were positive. The most common organisms isolated from urinary tract infections are gram-negative organisms. So the LAL assay is a rapid, simple and reliable method in determining of clinically significant gram-negative bacteriuria.
Key words : candidemia, endotoxin, D-arabinitol, Candida antigen, serological examination
Key words : candidemia, endotoxin, D-arabinitol, Candida antigen, serological examination Fig. 1 The value of fungal index and D- arabinitol/creatinine. Fungal index was mesured by Endotoxin D and Endospecy
More informationKey words : R-plasmid, Urinary tract infection, E. coli Fig. 1. MIC distribution against E. coli isolated from urinary tract (366 strains) and isolation - frequencies of drug-resistant strains Table 1.
More information1272 CHEMOTHERAPY MAR. 1975
1272 CHEMOTHERAPY MAR. 1975 VOL. 23 NO. 3 CHEMOTHERAPY 1273 Fig. 2 Minimal inhibitory concentration of aminoglycosides against 50 strains of Klebsiella Fig. 1 Minimal inhibitory concentration of aminoglycosides
More informationCHEMOTHERAPY APRIL 1992 Table 2. Concentration of meropenem in human prostatic fluid Table 1. Background of 21 chronic complicated UTI cases * NB + BPH, NB + Kidney tumor, NB + Kidney tuberculosis Table
More informationFig. 1. Structures of NM394, NAD-358 and NAD-245 Fig. 2. Typical HPLC chromatograms of NM394 in human plasma by organic solvent extraction method (a): Blank plasma (b): Plasma spiked with NM394 and internal
More informationCHEMOTHERAPY
CHEMOTHERAPY VOL.41 S-2 Laboratory and clinical evaluation of teicoplanin CHEMOTHERAPY AUG. 1993 VOL.41 S-2 Laboratory and clinical evaluation of teicoplanin Table 1. Comparative in vitro activity of teicoplanin
More informationFig. 1 Chemical structure of TE-031 Code number: TE-031 Chemical name: (-) (3R, 4S, 5S, 6R, 7R, 9R, 11R, 12R, 13S, 14R)-4-[(2, 6-dideoxy-3-C-methyl-3-
Fig. 1 Chemical structure of TE-031 Code number: TE-031 Chemical name: (-) (3R, 4S, 5S, 6R, 7R, 9R, 11R, 12R, 13S, 14R)-4-[(2, 6-dideoxy-3-C-methyl-3-O-methyl-a-L-ribo-hexopyranosyl) oxy]-14-ethyl-12,
More informationFig.1 MICs of penicillins against 24 strains of B. pertussis Fig.2 MICs of cepherns against 24 strains of B. pertussis Fig.3 MICs of macrolides against 24 strains of B. pertussis Fig.4 MICs of nalidixic
More informationVisual Evaluation of Polka-dot Patterns Yoojin LEE and Nobuko NARUSE * Granduate School of Bunka Women's University, and * Faculty of Fashion Science,
Visual Evaluation of Polka-dot Patterns Yoojin LEE and Nobuko NARUSE * Granduate School of Bunka Women's University, and * Faculty of Fashion Science, Bunka Women's University, Shibuya-ku, Tokyo 151-8523
More information400 46 THE JAPANESE JOURNAL OF ANTIBIOTICS 65 6 Dec. 2012 LVFX 100 mg 3 / 7 150 mg 2 / 7 2 2006 2008 9 LVFX PK PD 2009 7 100 mg 1 3 500 mg 1 1 AUC/MIC
Dec. 2012 THE JAPANESE JOURNAL OF ANTIBIOTICS 65 6 399 45 2012 11 5 LVFX 500 mg 1 1 20 Chlamydia trachomatis C. trachomatismycoplasma genitalium M. genitalium LVFX 1 500 mg 1 1 7 22 22 C. trachomatis 17
More informationCHEMOTHERAPY JUNE 1986
VOL. 34 S-3 CHEMOTHERAPY Fig. 1 Structural formula of L-105 CHEMOTHERAPY JUNE 1986 VOL. 34 S-3 CHEMOTHERAPY Table 1 Antibacterial spectra of L-105 against gram negative anaerobic rods Inoculum 106 cells/ml
More informationCHEMOTHERAPY
CHEMOTHERAPY CHEMOTHERAPY Table 1 Antibacterial activity of BRL 28500 against standard strains of bacteria Fig, 1 Sensitivity distribution of ABPC-resistant E. coli isolated from urinary tract Fig. 2 Sensitivity
More informationKey words: Surfactant, Tween, Legionella
Key words: Surfactant, Tween, Legionella Fig. 1 Inhibitory Activity of Various Tween Against Legionella pneumophila Fig. 2 Inhibitory Activity of Various Tween Against Legionella bozemanii Fig. 3 Inhibitory
More informationCHEMOTHERAPY AUG. 1982 VOL. 30 NO. 8 CHEMOTHERAPY Fig.1 Relation between various-closis of cefazolin and detection rate of organisms in heart blood of dying mice with E. coli and P. aeruginosa infection
More informationFig. 1 Clinical findings and extent of inflammation area in female urethrocystitis Fig. 2 Classification and distribution of female patients with blad
Key words: Female with bladder irritability, Subjective symptoms, Pyuria, Bacteriuria Fig. 1 Clinical findings and extent of inflammation area in female urethrocystitis Fig. 2 Classification and distribution
More informationA Nutritional Study of Anemia in Pregnancy Hematologic Characteristics in Pregnancy (Part 1) Keizo Shiraki, Fumiko Hisaoka Department of Nutrition, Sc
A Nutritional Study of Anemia in Pregnancy Hematologic Characteristics in Pregnancy (Part 1) Keizo Shiraki, Fumiko Hisaoka Department of Nutrition, School of Medicine, Tokushima University, Tokushima Fetal
More informationsemen quality or those without WBC in semen. In the patients with azoospermia and normal FSH levels (normogonadotropic azzospermia), the antibody (IgG
CLINICAL STUDIES OF UROGENITAL INFECTIONS WITH CHLAMYDIA TRACHOMA TIS Report 2. The Epidemiology of Chlamydial Infections in Okayama District in Japan and Detection of Antibodies to Chlamydiae in the Sera
More informationCHEMOTHERAPY FEB Table 1. Activity of cefpirome and others against clinical isolates
VOL.39 S-1 CHEMOTHERAPY FEB. 1981 Table 1. Activity of cefpirome and others against clinical isolates VOL.39 S-1 CHEMOTHERAPY FEB. 1991 72 M, 55.5 kg 66 F, 53 kg Chronic bronchitis Bronchopneumonia Peak
More informationTable 1 Survival rates of infected mice given antibiotic doses producing peak serum a) S. aurcus Smith Challenge dose :7 ~10 (5% mucin) CFU/mouse. LD50: 1 ~103 (5% mucin) CFU/mouse. Table 2 Survival rates
More informationTable 1.Resistance criteria Fig.1.The resistance rates of piperacillin,ceftazidime, cefsulodin,imipenem,aztreonam,gentamicin,tobramycin,amikacin,isepamicin,fosfomycin and ofloxacin against 2,793 strains
More informationFig. 1 Chemical structure of DL-8280
Fig. 1 Chemical structure of DL-8280 Fig. 2 Susceptibility of cl in ical isolates to DL4280 Fig. 5 Susceptibility of clinical isolates to DL-8280 Fig. 3 Susceptibility of clinical isolates to DL-8280 Fig.
More informationTable 1.Concentration of gatifloxacin (Middle-ear) Table 2.Concentration of gatifloxacin (Paranasal sinuses) Table 3.Concentration of gatifloxacin (Tonsil) Table 4.No.of patients studied Table 5.Background
More informationTable 1. Antibacterial spectrum SBT ABPC ABPC CPZ : sulbactamiampicillin : ampicillin : cefoperazone
Table 1. Antibacterial spectrum SBT ABPC ABPC CPZ : sulbactamiampicillin : ampicillin : cefoperazone (inoculum size= 106 CFU/ml) (Ĉ-lactamase producer : 2 strains) Fig. 1. Sensitivity distribution of
More informationCHEMOTHERAPY JUN Citrobacter freundii 27, Enterobacter aerogenes 26, Enterobacter cloacae 27, Proteus rettgeri 7, Proteus inconstans 20, Proteus
VOL. 32 S-4 CHEMOTHERAPY Fig. 1 Chemical structure of sodium cefoperazone Fig. 2 Chemical structure of sodium cefoperazone CHEMOTHERAPY JUN. 1984 Citrobacter freundii 27, Enterobacter aerogenes 26, Enterobacter
More informationTable 1 Sensitivity distribution of clinical isolates 1. Escherichia coli Inoculum size: 106cells/ml 2. Klebsiella pneumoniae 3. Enterobacter cloacae 4. Serratia marcescens Inoculum size: 106cells/nil
More informationKey words:fatty acid,plant oil,staphylococcus aureus,skin care, atopic dermatitis
Key words:fatty acid,plant oil,staphylococcus aureus,skin care, atopic dermatitis growth was monitored at 660 nm with a biophotorecorder. Table 1.Relative inhibitory effects of fatty acids,plant oils,
More information2) Goetz, A., Tsuneishi, N.: Application of molecular filter membranes to the bacteriological analysis of water, J. Am. Water Works Assn., 43 (12): 943-969,1951. 3) Clark, H.F. et al.: The membrane filter
More informationVOL. 23 NO. 3 CHEMOTHERAPY 1067 Table 2 Sensitivity of gram positive cocci isolated from various diagnostic materials Table 3 Sensitivity of gram nega
1066 CHEMOTHERAPY MAR. 1975 Table 1 Sensitivity of standard strains VOL. 23 NO. 3 CHEMOTHERAPY 1067 Table 2 Sensitivity of gram positive cocci isolated from various diagnostic materials Table 3 Sensitivity
More informationClostridium difficile ciprofloxacin, ofloxacin, norfloxacin Bifidobacterium Lactobacillus Lactobacillus Bacteroides fragilis B. fragilis C. difficile
Clostridium difficile ciprofloxacin, ofloxacin, norfloxacin Bifidobacterium Lactobacillus Lactobacillus Bacteroides fragilis B. fragilis C. difficile Key words: temafloxacin, TA-167, Bacteroides fragilis,
More informationKey Words: Klebsiella pneumoniae, CEP-AIS, MIC, "MBC", MIC of drugs in combination
Key Words: Klebsiella pneumoniae, CEP-AIS, MIC, "MBC", MIC of drugs in combination Fig. 1. The following three kinds of overnight broth cultures of 10 strains of Kl. pneumoniae were inoculated on the agar
More informationVOL.32 S-7 CHEMOTHERAPY Table 1 MIC of standard strains of CTRX Fig. 2 Cumulative curves of MIC S. aureus (26 strains )
CHEMOTHERAPY OCT. 1984 Fig. I Chemical structure of CTRX VOL.32 S-7 CHEMOTHERAPY Table 1 MIC of standard strains of CTRX Fig. 2 Cumulative curves of MIC S. aureus (26 strains ) CHEMOTHERAPY Fig. 3 Cumulative
More informationKey words: bacterial meningitis, Haemophilus influenzae type b, Streptococcus pneumoniae, rapid diagnosis, childhood
Key words: bacterial meningitis, Haemophilus influenzae type b, Streptococcus pneumoniae, rapid diagnosis, childhood Fig.1 Distribution of the cases with bacterial meningitis by age and pathogens Chiba
More informationCHEMOTHERAPY aureus 0.10, Enterococcus faecalis 3.13, Escherichia coli 0.20, Klebsiella pneumoniae, Enterobacter spp., Serratia marcescens 0.78, Prote
aureus 0.10, Enterococcus faecalis 3.13, Escherichia coli 0.20, Klebsiella pneumoniae, Enterobacter spp., Serratia marcescens 0.78, Proteus mirabilis 3.13, Proteus vulgaris 1.56, Citrobacter freundii 0.39,
More informationb) Gram-negative bacteria Fig. 2 Sensitivity distribution of clinical isolates : E. coli Fig. 3 Sensitivity distribution of clinical isolates : Pseudomonas Fig. 1 Sensitivity distribution of clinical isolates
More informationTable1MIC of BAY o 9867 against standard strains
Table1MIC of BAY o 9867 against standard strains Fig.2Cumulative and Distribution Curves of MIC (S.aureus 54 strains) 106cfu/ml Fig.3Correlogram of MIC (S.aureus 54 strains) CHEMOTHERAPY 451 Fig.4Cumulative
More informationCHEMOTHERAPY APR Fig. 1 Chemical structure of cefotetan (CTT, YM09330)
CHEMOTHERAPY APR. 1982 Fig. 1 Chemical structure of cefotetan (CTT, YM09330) VOL.30 S-1 CHEMOTHERAPY Fig. 2 Comparison of standard curves of CTT on various test organisms by cylinder plate method Column
More informationCHEMOTHERAPY APRIL 1992 VOL. 40 S- 1 Table 1-1. Comparative in vitro activity of meropenem against clinical isolates CNS: coagulase-negative staphylococci CHEMOTHERAPY APRIL 1992 Table 1-2. Comparative
More informationVOL. 34 S-2 CHEMOTH8RAPY 913
VOL. 34 S-2 CHEMOTH8RAPY 913 914 CHEMOTHERAPY APR. 1986 Fig. 1 Chemical structure of T-2588 and T-2525 T- 2588 pivaloyloxymethyl (+ )- (6 R, 7 R)-7-[(Z)-2- (2-amino- 4-thiazolyl)-2-methox yiminoacetamido]-3-[(
More informationcoccus aureus Corynebacterium sp, Haemophilus parainfluenzae Klebsiella pneumoniae Pseudornonas aeruginosa Pseudomonas sp., Xanthomonas maltophilia, F
VOL.43 S-1 coccus aureus Corynebacterium sp, Haemophilus parainfluenzae Klebsiella pneumoniae Pseudornonas aeruginosa Pseudomonas sp., Xanthomonas maltophilia, Flavobacter- Table 1. Concentration of grepafloxacin
More informationCHEMOTHERAPY DEC Table 1 Antibacterial spectra of T-1982, CTT, CMZ, CTX, CPZ and CEZ 106 CFU/ml Note: P; Peptococcus, S; Streptococcus, G; Gaffk
VOL. 30 S-3 CHEMOTHERAPY imeumoniae, Serratia marcescens, Proteus sp, CHEMOTHERAPY DEC. 1982 Table 1 Antibacterial spectra of T-1982, CTT, CMZ, CTX, CPZ and CEZ 106 CFU/ml Note: P; Peptococcus, S; Streptococcus,
More informationCHEMOTHERAPY FEB Table 1 Background of volunteers
CHEMOTHERAPY FEB. 1985 Table 1 Background of volunteers Table 3 Reproducibility of saisomicln In the EIA and the RIA Fig.1 Comparison of the EIA with the RIA or bioassay of sisomicin Table 4 Blood levels
More informationVOL.35 S-2 CHEMOTHERAPY Table 1 Sex and age distribution Table 2 Applications of treatment with carumonam Table 3 Concentration of carumonam in human
CHEMOTHERAPY Fig. 1 Chemical structure of carumonam Disodium(+)-(Z)-CCE1-(2-amino-4-thiazoly1)-2-[[(2S, -(carbamoyloxymethyl)-4-oxo-1-sulfonato-3-azetidinyll -2-oxoethylidene] amino] oxy] acetate 3S)-2
More informationKey words: Antibodies to Leptospira, Tokyo, Uveitis
Key words: Antibodies to Leptospira, Tokyo, Uveitis Fig. 1 Distribution of Antibody Titers in Age Decade Fig. 3 Distribution of Antibody Titers in each Strain Fig. 2 Correlation between Antibody Titers
More informationVOL. 43 NO. 4
VOL. 43 NO. 4 Fig. 1. Frequency of Enterococcus species from complicated UTI, 1988-1992. the number * of Enterococcus species/the number of cases with complicated UTI. Fig. 3 Epidemiologic characteristics
More informationCHEMOTHERAPY Table 1 Clinical effect of Sultamicillin
CHEMOTHERAPY CHEMOTHERAPY Table 1 Clinical effect of Sultamicillin CHEMOTHERAPY Fig. 1 MICs of sultamicillin against respiratory pathogenic Branhamella catarrhalis 62 strains, inoculum size 106CFU/m1 Fig.
More informationTable 1. Antibacterial activitiy of grepafloxacin and other antibiotics against clinical isolates
Table 1. Antibacterial activitiy of grepafloxacin and other antibiotics against clinical isolates Table 2-1. Summary of patients treated with grepafloxacin for respiratory infection 1) Out: outpatient,
More information1) i) Barber, M. et al.: Brit. Med J, 2, 565, 19'49. ii) Barber, M.F.G. J. Hayhoe and J. E. M. Whithead: Lancet, 1120 `1125, 1949.-2) Bergey: Bergey's Manual of Determinative Bacteriology 7 th Ed: (1958).-3)
More information2 94
32 2008 pp. 93 106 1 Received October 30, 2008 The purpose of this study is to examine the effects of aerobics training class on weight loss for female students in HOKURIKU UNIVERSITY. Seventy four female
More informationVOL.42 S-1
CHEMOTHERAPY APR. 1994 VOL.42 S-1 CHEMOTHERAPY APR. 1994 Table 1. Criteria for evaluation of clinical efficacy by the Japanese Society of Oral and Maxillo-Facial Surgeons Grades of symptoms and numerical
More informationTherapy for Asthenopia in Cases of Convergence Insufficiency Hiroko TAKASAKI, C.O.J., Nobuko INAGAMI, C.O.J., and Kayoko TAKENAWA, C.O.J.. Orthoptic c
Therapy for Asthenopia in Cases of Convergence Insufficiency Hiroko TAKASAKI, C.O.J., Nobuko INAGAMI, C.O.J., and Kayoko TAKENAWA, C.O.J.. Orthoptic clinic, Department of Ophthalmology, (Director: Prof
More informationCHEMOTHERAPY JUNE 1993 Table 1. Background of patients in pharmacokinetic study
CHEMOTHERAPY JUNE 1993 Table 1. Background of patients in pharmacokinetic study VOL. 41 S 1 Table 2. Levels (Đg/ml or Đg/g) of S-1006 in serum, bile, and tissue (gallbladder) after oral administration
More informationCHEMOTHERAPY JUNE 1987 Table1 Media used *BHIB, brain heart infusion broth (Difco); /3 -NAD, S -nicotinamidoadeninedinucleotide (Sigma Chemical Co.);
VOL.35 S-2 CHEMOTHERAPY Fig.1 Chemical structure of carumonam CHEMOTHERAPY JUNE 1987 Table1 Media used *BHIB, brain heart infusion broth (Difco); /3 -NAD, S -nicotinamidoadeninedinucleotide (Sigma Chemical
More informationCHEMOTHERAPY APR Fig. 2 The inactivation of aminoglycoside antibiotics by PC-904 Fig. 3 Serum concentration of PC-904 (1) Fig. 4 Urinary recover
VOL.26 S-2 CHEMOTHERAPY Gentamicin (GM), Dibekacin (DKB), Tobramycin Fig. 1 Protein concentration and protein binding rate Table 2 Protein binding rate of PC-904 in serum of healthy adults, and patients
More informationepidermidis, Enterococcus faecalis, Enterococcus Klebsiella pneumoniae, Proteus mirabilis, indolepositive Proteus spp., Enterobacter spp., Serratia
epidermidis, Enterococcus faecalis, Enterococcus Klebsiella pneumoniae, Proteus mirabilis, indolepositive Proteus spp., Enterobacter spp., Serratia Table 3. Overall clinical efficacy of cefozopran in
More informationCHEMOTHERAPY Fig. 1 Chemical structure of CXM-AX
Fig. 1 Chemical structure of CXM-AX NOV. 1986 Fig. 2 Sensitivity distribution of clinical isolates organisms (106 cells/ml) a Smurcus 27 strains d) P.m irabilis 15 strains b Ecol i 27 strains 111.morganii
More informationKey words: HIV screening kit, window period, p24antigen, enzyme-linked fluorescent assay
Key words: HIV screening kit, window period, p24antigen, enzyme-linked fluorescent assay Fig.1 @ A principle of assay The assay comprises of two reactions. The first reaction, for the detection of anti-hiv-
More informationCHEMOTHERAPY Proteus mirabilis GN-79 Escherichia coli No. 35 Proteus vulgaris GN-76 Pseudomonas aeruginosa No. 11 Escherichia coli ML-1410 RGN-823 Kle
VOL. 29 NO.8 CHEMOTHERAPY 865 CHEMOTHERAPY Proteus mirabilis GN-79 Escherichia coli No. 35 Proteus vulgaris GN-76 Pseudomonas aeruginosa No. 11 Escherichia coli ML-1410 RGN-823 Klebsiella pneumoniae GN-69
More informationVOL.30 S-1 CHEMOTHERAPY Table 1 Antibacterial activity of CTT against standard strains Table 2 Antibacterial activity of CTT against standard strains
CHEMOTHERAPY APR. 1982 Fig. 1 Chemical structure of cefotetan (CTT, YM09330) VOL.30 S-1 CHEMOTHERAPY Table 1 Antibacterial activity of CTT against standard strains Table 2 Antibacterial activity of CTT
More informationThe Journal of the Japan Academy of Nursing Administration and Policies Vol 7, No 2, pp 19 _ 30, 2004 Survey on Counseling Services Performed by Nursi
The Journal of the Japan Academy of Nursing Administration and Policies Vol 7, No 2, pp 19 _ 30, 2004 Survey on Counseling Services Performed by Nursing Professionals for Diabetic Outpatients Not Using
More informationFig.2. Sensitivity distribution of clinical isolates of S. epidermidis (24 strains, 106 CFU/ml) Staphylococcus aureus Staphylococcus epider- midis Ent
Fig.2. Sensitivity distribution of clinical isolates of S. epidermidis (24 strains, 106 CFU/ml) Staphylococcus aureus Staphylococcus epider- midis Enterococcus faecalis Klebsiella pneumoniae, Morganella
More informationJpn. J. Med. Mycol. Vol. 21, , 1980 ISSN
Jpn. J. Med. Mycol. Vol. 21, 274-285, 1980 ISSN 0583-0516 Fig. 1. Separation and purification procedures of cellular mannans from C. albicans and S. cereaisiae Jpn. J. Med. Mycol. Vol. 21 (No. 4), 1980
More informationJournal of Geography 116 (6) Configuration of Rapid Digital Mapping System Using Tablet PC and its Application to Obtaining Ground Truth
Journal of Geography 116 (6) 749-758 2007 Configuration of Rapid Digital Mapping System Using Tablet PC and its Application to Obtaining Ground Truth Data: A Case Study of a Snow Survey in Chuetsu District,
More informationStudies of Foot Form for Footwear Design (Part 9) : Characteristics of the Foot Form of Young and Elder Women Based on their Sizes of Ball Joint Girth
Studies of Foot Form for Footwear Design (Part 9) : Characteristics of the Foot Form of Young and Elder Women Based on their Sizes of Ball Joint Girth and Foot Breadth Akiko Yamamoto Fukuoka Women's University,
More information名称未設定-1
Study on Clinical Factors Affecting the Fungal Culture Test - Relevance of Dry Mouth - Yoshiko YAMAMURA, Yukihiro MOMOTA, Hideyuki TAKANO, Koichi KANI, Katsumi MOTEGI, Fumihiro MATSUMOTO, Masayuki AZUMA
More information(43) Vol.33, No.6(1977) T-239 MUTUAL DIFFUSION AND CHANGE OF THE FINE STRUCTURE OF WET SPUN ANTI-PILLING ACRYLIC FIBER DURING COAGULATION, DRAWING AND
(43) Vol.33, No.6(1977) T-239 MUTUAL DIFFUSION AND CHANGE OF THE FINE STRUCTURE OF WET SPUN ANTI-PILLING ACRYLIC FIBER DURING COAGULATION, DRAWING AND DRYING PROCESSES* By Hiroshi Aotani, Katsumi Yamazaki
More informationKey words: E. coli O 157: H7, fosfomycin, verotoxin, mouse infection
Key words: E. coli O 157: H7, fosfomycin, verotoxin, mouse infection Table 1. Bacterial cell counts in feces of mice infected with Esclwrichia coli O 157: H7 NK2 before and during oral dosing with fosfomycin
More informationFig.1 Structural formulas of 6059-S(I) and decarboxylated product (11) Fig.2 Standard curve of 6059-S by agar well method Medium:Trypto-soy agar Diluent:0.1M Phosphate buffer,ph 7.0 Fig.3 Standard curves
More information_念3)医療2009_夏.indd
Evaluation of the Social Benefits of the Regional Medical System Based on Land Price Information -A Hedonic Valuation of the Sense of Relief Provided by Health Care Facilities- Takuma Sugahara Ph.D. Abstract
More informationudc-2.dvi
13 0.5 2 0.5 2 1 15 2001 16 2009 12 18 14 No.39, 2010 8 2009b 2009a Web Web Q&A 2006 2007a20082009 2007b200720082009 20072008 2009 2009 15 1 2 2 2.1 18 21 1 4 2 3 1(a) 1(b) 1(c) 1(d) 1) 18 16 17 21 10
More information2種の(1→3)-β-D-グルカン測定試薬の真菌に対する反応性の比較
Comparison of the reactivity of two kinds of (1 3)- -D-glucan measurement reagents to fungi Tadashi Matsubayashi and Tomoyuki Yamazaki Measurements of (1 3)- -D-glucan are widely used for the diagnosis
More informationTable 1 Epidemiologic characteristics in the elderly patients with sepsis Table 2 Main underlying disease
Key words: elderly patient, sepsis, multiple organ failure (MOF) Table 1 Epidemiologic characteristics in the elderly patients with sepsis 1985-1994 Table 2 Main underlying disease Fig. 1 The yearly frequency
More informationKey words: Streptococcus pyogenes, Streptococcus dysgalactiae subsp. equisimilis, rapid diagnosis
Key words: Streptococcus pyogenes, Streptococcus dysgalactiae subsp. equisimilis, rapid diagnosis Table 1 Strains evaluated Table 3 Comparison of sensitivity (CFU/mL) of 8 rapid diagnostic kits for detecting
More informationCHEMOTHERAPY
CHEMOTHERAPY CHEMOTHERAPY Table 1 Antibacterial activity of Sulbactam/CPZ against standard strains MIC mg/ml Inoculum size 106 CFU/ml * Sulbactam/CPZ= 1: 1 ** Concentration of Sulbactam+ CPZ CHEMOTHERAPY
More information1. Precise Determination of BaAl2O4 Cell and Certification of the Formation of Iron Bearing Solid Solution. By Hiroshi UCHIKAWA and Koichi TSUKIYAMA (
1. Precise Determination of BaAl2O4 Cell and Certification of the Formation of Iron Bearing Solid Solution. By Hiroshi UCHIKAWA and Koichi TSUKIYAMA (Central Research Laboratory, Onoda Cement Co., Ltd.,
More information320 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /., No.1, -,* -,/ (,**1) 8 * ** *** * ** *** E#ect of Superheated Steam Treatment on the Preservation an
320 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /., No.1, -,* -,/ (,**1) 8 * ** *** * ** *** E#ect of Superheated Steam Treatment on the Preservation and Sensory Characteristics of Buckwheat Noodles Kazuhiro
More informationA comparison of abdominal versus vaginal hysterectomy for leiomyoma and adenomyosis Kenji ARAHORI, Hisasi KATAYAMA, Suminori NIOKA Department of Obstetrics and Gnecology, National Maizuru Hospital,Kyoto,
More informationThe clinical characteristics of Mycoplasma pneumoniae pneumonia in children younger than 6 years old Nobue Takeda1,2),Tomomichi Kurosaki1),Naruhiko Ishiwada2),Yoichi Kohno2) 1)Department of Pediatrics,Chiba
More informationAdult Attachment Projective AAP PARS PARS PARS PARS Table
PARS PARS PARS PARS IWM IWM SATSAT Adult Attachment Projective AAP PARS PARS PARS PARS Table PARS PARS PARS PARS PARS AAP George, West, & Pettem Bowlby Bowlby George et al.,, Pp. - Geroge & West, PARS
More informationMikio Yamamoto: Dynamical Measurement of the E-effect in Iron-Cobalt Alloys. The AE-effect (change in Young's modulus of elasticity with magnetization
Mikio Yamamoto: Dynamical Measurement of the E-effect in Iron-Cobalt Alloys. The AE-effect (change in Young's modulus of elasticity with magnetization) in the annealed state of iron-cobalt alloys has been
More information市区町村別平均寿命の全国順位の変化からみた青森県市町村平均寿命の解析
Analysis of the life expectancy at birth of the municipalities in Aomori Prefecture by the change in a nationwide order of the life expectancy at birth Koichi Takemori 1) Seiji Mikami2) Naomi Kudo 1) Yutaka
More informationValidation of a Food Frequency Questionnaire Based on Food Groups for Estimating Individual Nutrient Intake Keiko Takahashi *', Yukio Yoshimura *', Ta
Validation of a Food Frequency Questionnaire Based on Food Groups for Estimating Individual Nutrient Intake Keiko Takahashi *', Yukio Yoshimura *', Tae Kaimoto *', Daisuke Kunii *', Tatsushi Komatsu *2
More informationKey words: Disinfectants, Gram negative rods, Bactericidal effect P. aeruginosa 1, P. fluorescens 20 P. putida 179, P. cepacia 216 P. maltophilia 227,
Key words: Disinfectants, Gram negative rods, Bactericidal effect P. aeruginosa 1, P. fluorescens 20 P. putida 179, P. cepacia 216 P. maltophilia 227, P. putrefaciens 279 A. faecalis 120, A. ordrans 114
More informationStudy on Application of the cos a Method to Neutron Stress Measurement Toshihiko SASAKI*3 and Yukio HIROSE Department of Materials Science and Enginee
Study on Application of the cos a Method to Neutron Stress Measurement Toshihiko SASAKI*3 and Yukio HIROSE Department of Materials Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa-shi,
More informationCHEMOTHERAPY NOV S. aureus, S. epidermidis, E. coli, K. pgeumoniae, E. cloacae, S. marcescens, P. mirabilis, Proteus, P. aeruginosa Inoculum siz
VOL.33 S-5 CHEMOTHERAPY 381 Fig. 1 Chemical structure of HAPA-B Chemical name 1-N-[(2S)-3-Amino-2-hydroxypropiony1]-4-0-(6-amino- 6-deoxy-a-D-glucopyranosyl)-6-013-deoxy-4-C-methyl- 3-(methylamino)-ƒÀ-L-arabinopyranosyl]-2-deoxystreptamine
More informationTable 1 Muscle weight (g) and those ratio (%) in M. quadriceps femoris Table 2 Cross-sectional area (mm2) and those ratio (%) in M, quadriceps femoris
Table 1 Muscle weight (g) and those ratio (%) in M. quadriceps femoris Table 2 Cross-sectional area (mm2) and those ratio (%) in M, quadriceps femoris Fig. 1 Comparison of cross-sectional area in human
More information366 12 THE JAPANESE JOURNAL OF ANTIBIOTICS 65 6 Dec. 2012 1 8 DNA 2,3 16 12 20 171 2008 12 2010 11 2 3,558 4.44% 1.65% 1.17% 90% 9 Escherichia coli -
Dec. 2012 THE JAPANESE JOURNAL OF ANTIBIOTICS 65 6 365 11 sita oxacin 1 1 1 1 1 1 2 2 3 3 1 1 1 2 3 2012 9 14 sita oxacin STFX 50 mg 10% 2008 1 2008 12 2010 11 2 STFX 1,452 91.4% 1,235/1,351 95.9% 466/486
More informationEQUIVALENT TRANSFORMATION TECHNIQUE FOR ISLANDING DETECTION METHODS OF SYNCHRONOUS GENERATOR -REACTIVE POWER PERTURBATION METHODS USING AVR OR SVC- Ju
EQUIVALENT TRANSFORMATION TECHNIQUE FOR ISLANDING DETECTION METHODS OF SYNCHRONOUS GENERATOR -REACTIVE POWER PERTURBATION METHODS USING AVR OR SVC- Jun Motohashi, Member, Takashi Ichinose, Member (Tokyo
More informationVOL. 23 NO. 3 CHEMOTHERAPY 1379 Table 1 Susceptibility of clinical isolated strains to Tobramycin
VOL. 23 NO. 3 CHEMOTHERAPY 1379 Table 1 Susceptibility of clinical isolated strains to Tobramycin 1380 CHEMOTHERAPY MAR. 1975 Table 2 Susceptibility of isolated Pseudomonas aeruginosa to various antibiotics
More informationRelationship between Men's Commuting Clothes and their Awareness toward Work Setsuko Yajiri*, Tomoko Takaoka**, Machiko Morita*** and Shigeo Kobayashi
Relationship between Men's Commuting Clothes and their Awareness toward Work Setsuko Yajiri*, Tomoko Takaoka**, Machiko Morita*** and Shigeo Kobayashi*** *Showa Womens' Junior College **Hokkaido Womens'
More informationAdams, B.N.,1979. "Mate selection in the United States:A theoretical summarization," in W.R.Burr et.al., eds., Contemporary Theories about the Family, Vol.1 Reserch - Based Theories, The Free Press, 259-265.
More informationJpn.J.Leprosy 67, (1998)
Jpn.J.Leprosy 67,287-291(1998) Fig.1. Macroscopic appearance of the cultured slides stained by the Ziehl-Neelsen method. Up to bottom: kept at 4 C, 18 weeks, cultured for 6 weeks, 8 weeks, 10 weeks, 12
More information988 CHEMOTHERAPY NOV. 1971
988 CHEMOTHERAPY NOV. 1971 VOL. 19 NO. 8 CHEMOTHERAPY 989 Effect of medium-ph and inoculum size on activity of SB-PC heart infusion agar, mcg/ml Sensitivity distribution of Staphylococci to SB-PC in surgical
More informationEVALUATION OF NOCTURNAL PENILE TUMESCENCE (NPT) IN THE DIFFERENTIAL DIAGNOSIS OF IMPOTENCE Masaharu Aoki, Yoshiaki Kumamoto, Kazutomi Mohri and Kazunori Ohno Department of Urology, Sapporo Medical College
More informationKey words : 7432-S, Oral cephem, Urinary tract infection Fig. 1. Chemical structure of 7432-S.
Key words : 7432-S, Oral cephem, Urinary tract infection Fig. 1. Chemical structure of 7432-S. Table 1. Clinical summary of acute uncomplicated cystitis patients treated with 7432-S UTI : Criteria by the
More informationA comparative study of the team strengths calculated by mathematical and statistical methods and points and winning rate of the Tokyo Big6 Baseball Le
Powered by TCPDF (www.tcpdf.org) Title 東京六大学野球リーグ戦において勝敗結果から計算する優勝チームと勝点 勝率との比較研究 Sub Title A comparative study of the team strengths calculated by mathematical and statistical methods and points and winning
More informationTable 1 Patients with various renal function * Ccr, Creatinine clearance ml/min per 1. 48 m2 ** C.V.D., Cerebral vascular disease ; C.R F., Chronic renal failure ; H.D., Hemoclialysis ; D., Dialyzer ;
More informationVOL.32 S-9 CHEMOTHERAPY Table 1 Minimum inhibitory concentrations of AC-1370, CPZ and CAZ Table 2 Efficacy of AC-1370 and CPZ against systemic infections in mice *Inoculum size: 106 cells/ml * 95% confidence
More informationTable 1 Antibacterial spectra of CPM and other antimicrobials against anaerobes Fig. 1 In vitro activity of CPM and other antibiotics against B. fragilis (136 strains) Fig. 2 In vitro activity of CPM and
More informationStaphylococcus sp. K.pneumoniae P.mirabilis C.freundii E. cloacae Serratia sp. P. aeruginosa ml, Enterococcus avium >100ƒÊg/ml
CHEMOTHERAPY SEPT. 1992 cefoperazone ceftazidime (CAZ), imipenem (IPM) Staphylococcus sp., Enterococcus (CPZ), faecalis, Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, Enterobacter cloacae,
More information1) K. J. Laidler, "Reaction Kinetics", Vol. II, Pergamon Press, New York (1963) Chap. 1 ; P. G. Ashmore, "Catalysis and Inhibition of Chemical Reactio
1) K. J. Laidler, "Reaction Kinetics", Vol. II, Pergamon Press, New York (1963) Chap. 1 ; P. G. Ashmore, "Catalysis and Inhibition of Chemical Reactions", Butterworths, London (1963) Chap. 7, p. 185. 2)
More information