研究成果報告書
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2 2. Ohara, H., Matsumoto, H., Ito, K., Iwai, K., Sato, K. (2007) Comparison of quantity and structures of hydroxyproline-containing peptides in human blood after oral ingestionn of gelatin hydrolysates from different sources. J. Agric. Food Chem., 55, Ichikawa, S., Morifuji, M., Ohara, H., Matsumoto, H., Takeuchi, Y., Sato, K. (2010) Hydroxyproline-containing dipeptides and tripeptides quantified at high concentration in human blood after oral administration of gelatin hydrolysate. Int. J. Food Sci. Nutri. 61, Ichikawa, S., Morifuji, M., Ohara, H., Matsumoto, H., Takeuchi, Y., Sato, K. (2010) Hydroxyproline-containing dipeptides and tripeptides quantified at high concentration in human blood after oral administration of gelatin hydrolysate. Int. J. Food Sci. Nutri. 61, Shigemura, Y., Akaba, S., Kawashima, E.., Park E.-Y. Nakamura, Y., Sato, K. (2011) Identification of a novel food-derived collagen peptide, hydroxyprolyl-glycine, in human peripheral blood by pre-column derivatisation with phenyl isothiocyanate. Food Chemistry 5-20 g μm proly yl-hydroxyproline (Pro-Hyp) hydroxyprolyl-glycine (Hyp-Gly) 1-4) Pro o-hyp, Hyp-Gly 4, 5) ) Pro-H Hyp 1. Iwai, K., Hasegawa, T., Taguchi, Y., Morimatsu, F., Sato, K., Nakamura, Y., Higashi, A., Kido, Y., Nakabo, Y., Ohtsuki, K. (2005) Identification of food-derived collagen peptides in human blood after oral ingestion of gelatin hydrolysates. J. Agric. Food Chem., 53, , Shigemura, Y., Iwai, K., Morimatsu, F., Iwamoto, T., Mori, T., Oda, C. Taira, T., Park, E. Y., Nakamura, Y., Sato, K. (2009) Effect of prolyl-hydroxyproline (Pro-Hyp), a food-derived collagen peptide in human blood, on growth of fibroblasts from mouse skin. J. Agric. Food Chem., 57 (2), Pro-Hyp Pro o-hyp 7.
3 BALB/cAJ Jcl 10μL L 2,4-dinitrofluorobenzene (DNFB) 0.2% DNFB 9 18 Biomusher (Nippi) Pro-Hyp, HypGly (LC-MS/MS: 3200QTRAP, AB Sciex) multi reaction monitoring (MRM) H HPLC MRM 6-aminoquinolyl-N-hydroxy (AccQ) HPLC BalbC Shigemuraa et al., 2009 Pro-H Hyp Pro-Hyp PBS 75 5% Pro-Hyp p sccinimidil carbamate LC-MS/MS Shi igemura et al., 2009 Pro-Hyp, Hyp-Gly MTT T RN NA RT-PC CR (PEPT1, PEPT2, PHT1, Ci1),V 3(V) ) (Col5a3) p75ntr GAP PDH Pro-Hyp Pro-Hyp Hyp-Gly Hyp-Gly Pro-Hypp (WT) (CT) H Hyp-Gly Pro-Hyp DNFB Pro o-hyp DNFB Pto-H Hyp P Pro-Hyp DNFB DNF FB Pro-Hyp Pro-H Hyp
4 Pr ro-hyp Hyp-Gly Hyp-Gly Hyp-Gly Pro-H Hyp Pro-Hyp Pro-H Hyp Pro-Hyp Pro-Hy yp Pro-Hyp Pro-Hyp Pro-Hyp 6 Pro-Hyp Pro-Hyp 7 (Ogc) 1 (S Sub) GA APDH P PEPT1 RT-P PCR 8 (Ogc) 1 (S Sub) PEP PT2 P PHT1 RT-PC C. Pro-H Hyp Pro-H Hyp Pro-Hy yp 9 (Ogc) 1 (S Sub) Ci1 1 C Col5a3 RT-P PCR
5 7-9 PEPT1, PEPT2, Ci1 6 P Pro-Hyp PHT1 1 PHT1 His p7 75NTR P Pro-Hyp Pro-Hyp Pro-Hyp 10 p75ntr 10 p75ntr 11 Pro-Hyp, Hyp-Gly Pro-Hyp Pro-Hyp 4 1. Shigemura, Y., Akaba, S., Kawashima, E.., Park E.-Y. Nakamura, Y., Sato, K. (2011) Identification of a novel food-derived collagen peptide, hydroxyprolyl-glycine, in human peripheral blood by pre-column derivatisation with phenyl isothiocyanate. Food Chemistry, 129, (2 2011) グのための素素材 コラーーゲンペプチドを中心に.F Food Style 21, 15, Shigemura,Y., Nakaba, M., Shiratsuchi, E., Suyama, M.., Yamada, M., Kiyono, T. Fukamizu, K., Park, E. Y., Nakamura, Y.., Sato, K. (2012) Identification of food-derived elastin peptide, prolyl-glycine (Pro-Gly), in human blood after ingestion of elastin hydrolysate. J. Agric. Food Chem., 60, Shigemura, Y.., Kubomura, D., Sato, Y., Sato, K. (2014) Dose-dependent changes in the levels of free and peptide forms of hydroxyprolin ne in human plasma after collagen hydrolysate ingestion. Food Chemistry 159, ) Sato, K. (2011) Impact of extracellular matrix protein hydrolysatess on human health. 102nd AOCS Annual Meeting & Expo. Cincinnati, USA, May Sato, K. (2011) Food-derived collagen peptides in human blood-structure and biological functions. ICOFFF 2011, Taipei, Chinese Taipei, November (2 2012) 12
6 (2013) (2013) Satou, K. (2013) Incorporation of food-derived collagen peptides, Pro-Hyp and Hyp-Gly, into primary cultured fibroblast from mice skin possible trigger for growth of fibroblast. ISNFF Annual Conference, Taipei, Nov (2014) Pro-Hyp Sato, K. Proteins and peptides for complicated disease type. In Bioactive Food Proteins and Peptides. Applicationa in Human Health Hettirarachchy, N. S., Sato, K., Marshall, M. R., Kannan, A. Edit. CRC Press. Taylor & Francis Group, Boca Raton, FL, USA. pp Sato, K., Shigemura, Y. (2012) Effect of marine collagen peptide on skin condition. In Marine Cosmeceuticals Trends and Prospective Kim, S.-K. Edit. CRC Press, New York, pp Shiratsuchi, E., Nakaba, M., Shigemura, Y., Yamada, M., Sato, K. (2013) Fish-elastin hydrolysate. In Marine Proteins and Peptides Kim, S.-K. Edit. Wiley-Blackwell, pp pp (1) SATO, Kenji (2) SHIGEMURA Yasutaka (3)
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No. 21 2001 8 Abstract The purpose of this study is to analyze the current validity of the high commitment management model, which has been widely used in the USA and Europe. This model has shown efficiency
