164 種苗放流の現状 2009 Oncorhynchus mykiss , Paralichthys olivaceus Pagrus major 34,

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1 163 日本の希少魚類の現状と課題 59(2): 在来渓流魚 (イワナ類,サクラマス類 ): 利用, 増殖, 保全の現状と課題 Present status and future conservation of fluvial charr (Salvelinus spp.) and salmon (Oncorhynchus spp.) populations in Japanese mountain streams Salvelinus 2 6 Oncorhynchus masou 3 Salvelinus malma malma S. m. miyabei S. leucomaenis leucomaenis Oncorhynchus masou masou 1a S. l. pluvius 1b S. l. japonicus S. l. imbrius O. m. ishikawae O. masou subsp. Kawanabe, 1989 図 1. Salvelinus leucomaenis pluvius a Oncorhynchus masou masou b a b Fausch et al., 2009 渓流魚の水産的価値 t 89.2 t 81.5% 79.8%

2 164 種苗放流の現状 2009 Oncorhynchus mykiss , Paralichthys olivaceus Pagrus major 34, ,000 在来個体群の現状と保全上の問題点 Kawamura et al., 2007 Kubota et al., 2007 Kikko et al., 2008a, b Sato et al., Wofford et al., 2005 Kawamura et al., 2007 Kubota et al., 2007 Sato et al., 2010 Sato and Harada, 2008 Sato, 2006 Morita and Yamamoto, 2000 Sato and Harada, Kawamura et al., 2007 Kubota et al., 2007 Kikko et al., 2008a, b Frankham et al., 2002 DNA

3 165 ゾーニング管理 ; Yamamoto et al., 2004; Kubota et al., 2007; Kikko et al., 2008a, b; Sato et al., 2010; 2011 図 2.

4 166 在来個体群保全の最近の研究と実践の事例 PVA Tsuboi et al % 78.1% % 3.4% 2.8% 図 3. おわりに Fausch et al 引用文献 Fausch K. D., B. E. Rieman, J. B. Dunham, M. K. Young and D. P. Peterson Invasion versus isolation: trade-offs in managing native

5 167 salmonids with barriers to upstream movement. Conserv. Biol., 23: Frankham R., J. D. Ballou and D. A. Briscoe Introduction to conservation genetics. Cambridge University Press, Cambridge. 617 pp pp 2011 DNA : 1 4 Kawamura K, M. Kubota, M. Furukawa and Y. Harada The genetic structure of endangered indigenous populations of the amago salmon, Oncorhynchus masou ishikawae, in Japan. Conserv. Genet., 8: Kawanabe, H Japanese char(r(r))s and masu-salmon problems: a review. Physiol. Ecol. Japan, Spec. vol., 1: Kikko, T., Y. Kai, M. Kuwahara and K. Nakayama. 2008a. Genetic diversity and population structure of white-spotted charr (Salvelinus leucomaenis) in the Lake Biwa water system inferred from AFLP analysis. Ichthyol. Res., 55: Kikko, T., M. Kuwahara, K. Iguchi, S. Kurumi, S. Yamamoto, Y. Kai and K. Nakayama. 2008b. Mitochondrial DNA population structure of whitespotted charr (Salvelinus leucomaenis) in the Lake Biwa water system. Zool. Sci., 25: pp : Kubota, H., T. Doi, S. Yamamoto and S. Watanabe Genetic identification of native populations of fluvial white-spotted charr Salvelinus leucomaenis in the upper Tone River drainage. Fish. Sci., 73: Morita K. and S. Yamamoto Occurrence of a deformed whitespotted charr, Salvelinus leucomaenis (Pallas), population on the edge of its distribution. Fish. Manag. Ecol., 7: pp pp pp pp tokei/census/fc/2008/index.html , Sato, T Occurrence of deformed fish and their fitness related traits in Kirikuchi charr Salvelinus leucomaenis japonicus, the southernmost population of the genus Salvelinus. Zool. Sci., 23: Sato, T. and Y. Harada Loss of genetic variation and effective population size of Kirikuchi charr: implications for the management of small, isolated salmonid populations. Anim. Conserv., 11: Sato, T, T. Demise, H. Kubota, M. Nagoshi and K. Watanabe Hybridization, isolation and low genetic diversity of Kirikuchi charr, the southernmost populations of the genus Salvelinus. Trans. Am. Fish. Soc., 139: pp : pp pp Tsuboi, J., T. Iwata, K. Morita, S. Endou, H. Oohama and K. Kaji. In press. Strategies for the conservation of isolated populations: lessons from Japanese stream salmonids. Freshw. Biol., 58. Yamamoto, S., K. Morita, S. Kitano, K. Watanabe, I. Koizumi and K. Maekawa Phylogeography of white-spotted charr (Salvelinus leucomaenis) inferred from mitochondrial DNA sequences. Zool. Sci., 21: naisuimen/2310/naisuimen29.html DNA Wofford J. E. B., R. E. Gresswell and M. A. Banks Influence of barriers to movement on within-watershed genetic variation of coastal cutthroat trout. Ecol. Appl., 15: Tomoyuki Nakamura [email protected] Daisuke Kishi, Tetsuya Tokuhara : [email protected]. lg.jp, [email protected] Hitoshi Kubota [email protected] Takeshi Kikko kikkou-takeshi@pref. shiga.lg.jp Jun-ichi Tsuboi [email protected]

6 168 59(2): ネコギギ: 積極的保全に向けたアプローチ Pseudobagrus ichikawai: toward active conservation strategies Pseudobagrus ichikawai 1957 Okada and Kubota, IB 13 cm 4, Watanabe, 1994b, cm 6 7 cm 3 10 cm Watanabe, 1994a, 生息上の脅威河川改修災害災害復旧工事 Sagawa et al., 2011 ダム堰堤建設 2005 外来種と魚病 Pseudobagrus nudiceps

7 cm html Watanabe, 水質汚染流量減少底質変動違法採集保護対策と展望保護管理指針から積極的な保全へ三重県の場合 A B C 4 A B A

8 生態調査に基づく実効的な対策美濃加茂市の場合 1990 Watanabe, 1994b; Watanabe, Watanabe, 生息場所の大規模改変にあたって豊川の場合 129 m cbr.mlit.go.jp/shitara/ 10

9 謝辞引用文献 pp : pp papers/pdfs/nekoconguidv5-3s.pdf pp pp I 1999 pp (6) 9 20 Okada, Y. and S. Kubota Description of a new cat-fish Coreobagrus ichikawai, with an emendation of the genus Coreobagrus Mori. Japan. J. Ichthyol., 5: Sagawa, S., T. Tashiro, J. Negishi and Y. Kayaba Effects of catastrophic floods on daytime interstitial habitat of bagrid catfish Pseudobagrus ichikawai. Rikunomizu (Limnology in Tokai Region of Japan), 44: (8) pp (5) Watanabe, K. 1994a. Growth, maturity and population structure of the bagrid catfish, Pseudobagrus ichikawai, in the Tagiri River, Mie Prefecture, Japan. Japan. J. Ichthyol., 41: Watanabe, K. 1994b. Mating behavior and larval development of Pseudobagrus ichikawai (Siluriformes: Bagridae). Japan. J. Ichthyol., 41: Pseudobagrus ichikawai pp 1997 pp (6) 2 8 Watanabe, K Diel activity and reproductive territory of the Japanese bagrid catfish, Pseudobagrus ichikawai. Environ. Biol. Fish., 81: Watanabe, K Sudden crash of a local population of the endangered bagrid catfish Pseudobagrus ichikawai. Ichthyol. Res., 56: pp Katsutoshi Watanabe [email protected] Seiichi Mori [email protected]

Furukawa et al. (2004) fms13 Takagi et al. (2001) Tru-17 16 7) PCR 8) 3 DNA 3 9 10 HE 11) PIC 12) 13) ( ) 1 4 PIT 14) 2011 5 1 6 11 DNA mtdna msdna DN

Furukawa et al. (2004) fms13 Takagi et al. (2001) Tru-17 16 7) PCR 8) 3 DNA 3 9 10 HE 11) PIC 12) 13) ( ) 1 4 PIT 14) 2011 5 1 6 11 DNA mtdna msdna DN 1 2 3 4 DNA ms DNA 1) mt DNA 2) 2 DNA DNA 2011 3 5 3 1 DNA ALC 3) 523 820 822 2 DNA 3 4 msdna 5) Kai et al. (2005) 11 6) f61 f100 f60 f112 f86 f1770 f178 f160 f153 f204 f65 Cui et al. (2005) 3 Cst-6 Cst-7

164 種 苗 放 流 の 現 状 2009 Oncorhynchus mykiss , Paralichthys olivaceus Pagrus major 34,

164 種苗放流の現状 2009 Oncorhynchus mykiss , Paralichthys olivaceus Pagrus major 34,