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1 , No. 169, *1 *2 * * a1970 (IHN) IHN (PVP-I) (Amend and Pietsch 1972) IHN PVP-I PVP-I IHN IHN IHN IHN 1990 B 8

2 (VHS) (EHN) IHN 2002 (ISA) ISA 2002b

3 , No. 169, Aeromonas salmonicida 1981, 1991a, 1191b, 1992, Nomura et al. 1992a, 1992b (IHNV)CSV OMV 1988, Yoshimizu et al. 1989

4 1. Aeromonas salmonicida ,101 1, , , (BKD) 1987 ELISA IHNV A. salmonicida (Groot and Alderdice 1985) (Kobayashi and Yamamoto 1987) IHNV IHNV IHNV (Yoshimizu et al., 1989)PVP-I 1961 (Amend 1974) PVP-I 10% (Fowler and Banks 1990, 1991, 1991) -4-

5 , No. 169, 2003 PVP-I 25 ppm 50 ppm 15 PVP-I (Capman and Rogers 1992) 0.5m Nomura BKD

6 -6- Renibacterium salmoninarum PVP-I BKD IHN PVP-I BKD PCR IFA ELISA ELISA (1990) (1988) Jorquera et al. (2002) (Kimura and Yoshimizu 1991, Muroga 2001) W sec/cm IHN

7 , No. 169, W sec/cm 2 Kasai and Yoshimizu mg/l

8 4 (Bullock and Stuckey 1975) BKD KDM-2 3 IFA PCR A. salmonicida (Stanley et al. 2002) Stanley et al. (2002) PCR DNA (NCBV) DNA A. salmonicida CBB (Cipriano and Bertolini 1988, Cipriano et al. 1992, Nomura et al. 1994) IHN Cipriano et al. (1996) A. salmonicida -T -T Bullock et al. (1997) -8-

9 , No. 169, 2003 Amend, D. F Comparative toxicity of two iodophors to rainbow trout eggs. Tran. Am. Fish. Soc., 103: Amend, D. F. and J. P. Pietsch Virucidal activity of two iodophors to salmonid viruses. J. Fish. Res. Bd. Canada, 29: Bullock, G. L. and H. M. Stuckey Aeromonas salmonicida: Detection of asymptomatically infected trout. Prog. Fish-Cult., 37: Bullock, G. L., S. T. Summerfelt, A. C. Noble, A. L. Weber, M. D. Durant and J. A. Hankins Ozonation of a recirculating rainbow trout culture system I. Effects on bacterial gill disease and heterotrophic bacteria. Aquaculture, 158: Chapman, P. F. and R. W. Rogers Decline in iodine concentration of iodophor during water hardening of salmonid eggs and methods to reduce this effect. Prog. Fish-Cult., 54: Cipriano, R. C. and J. B. Bertolini Selection for virulence in the fish pathogen Aeromonas salmonicida using Coomassie Brilliant Blue agar. J. Wildlife Diseases, 24: Cipriano, R. C., L. A. Ford, C. E. Starliper, J. D. Teska, J. T. Nelson and B. N. Jensen Control of external Aeromonas salmonicida: Topical disinfection of salmonids with chloramine-t. J. Aquat. Animal Health, 8: Cipriano, R. C., L. A. Ford, J. D. Teska, and L. E. Hale Detection of Aeromonas salmonicida in the mucus of salmonid fishes. J. Aquat. Animal Health, 4: Fowler, L. G. and J. L. Banks Iodophor toxicity to eggs and fry of fall chinook salmon. Prog. Fish-Cult., 52: Fowler, L. G. and J. L. Banks A safe level of iodophor for treating eggs of fall chinook salmon during water hardening. Prog. Fish-Cult., 53: Groot, E. P., and D. F. Alderdice Fine structure of the external egg membrane of five species of Pacific salmon and steelhead trout. Can. J. Zool., 63: IPN 25: : 9-20 Jorquera, M. A., G. Valencia, M. Eguchi, M. Katayose and C. Riquelme

10 Disinfection of seawater for hatchery aquaculture systems using electrolytic water treatment. Aquaculture, 207: Kasai, H. and M. Yoshimizu Disinfection of water for aquaculture. Fish. Sci., 68, Supl. 1: : : 2-6. Kimura, T. and M. Yoshimizu Viral diseases of fish in Japan. Ann. Rev. Fish. Dis., 1: : pp (BKD) 35 61p : Kobayashi, W. and T. Yamamoto Light and electron microscopic observations of sperm entry in the chum salmon egg. J. Exp. Zool., 243: Muroga, K Viral and bacterial diseases of marine fish and shellfish in Japanese hatcheries. Aquaculture, 202: p : a 446: b 27 (27 th Annual Eastern Fish Health Workshop) 169: Nomura, T Bacterial diseases of freshwater fishes of Hokkaido. Sci. Rep. Hokkaido Fish Hatchery, 48: (Oncorhynchus keta) (Oncorhynchus gorbuscha) (Oncorhynchus masou) (Onocorhynchus nerka) Aeromonas salmonicida 16:

11 , No. 169, a Aeromonas salmonicida 26: b Aeromonas salmonicida 26: Aeromonas salmonicida 27: Nomura, T., M. Yoshimizu and T. Kimura. 1992a. An epidemiological study of furunculosis in salmon propagation. In Proceedings of the Oji International Symposium on Salmonid Diseases (edited by T. kimura). Hokkaido University Press, Sapporo. pp Nomura, T., M. Yoshimizu and T. Kimura. 1992b. The epidemiological study of furunculosis in salmon propagation. NOAA Tech. Rep., 111: Nomura, T., M. Yoshimizu, S. Moki and Y. Ezura Existence of non-agglutinating Aeromonas salmonicida subsp. salmonicida in strains isolated from salmonids in Yamagata prefecture, Japan. Sci. Rep. Hokkaido Salmon Hatchery, 48: [PVP ], 3: Aeromonas salmonicida, Vibrio anguillarum V. ordalii Stanley, C., M. Hiney, D. Gilroy, R. Powell, D. Padley and P. Smith Furunculosis-inducing ability of microcosms seeded with Aeromonas salmonicida correlates with colony-forming ability but not with PCR and ELISA data. Aquaculture, 210: : : : : : :

12 21: Yoshimizu, M., M. Sami and T. Kimura Survivability of infectious hematopoietic necrosis virus in fertilized eggs of masu salmon and chum salmon. J. Aquat. Animal. Health., 1: : Yoshimizu, M., T. Nomura, T. Awakura, Y. Ezura and T. Kimura Prevalence of pathogenic fish viruses in anadromous masu salmon (Oncorhynchus masou) in the northern part of Japan, Physiol. Ecol. Japan, Spec. 1: IHNV IHNV 57:

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