0.5mg (mg 2mg 3mg 50mg 00mg 4mg mg 0.5mg/g 0.5mg/g mg/g 2mg/g mg mg 00mg 5mg 0mg 0mg 0mg 65mg/g 5mg L- 200mg 4mEq/g 2.5mEq 5mEq 50mg 00mg 05mg 25mg 00

042007 6 4 2 2 7 4 283 2 0 7 337 3 4 9 84 3 0 5 355 4 4 5 82 4 7 5 249 5 3 3 5 4 22 75 2 0 6 3 7 3 7 9 4 5 4 76 4 0 6 5 5 2 5 7 22 2 3 0 9 9 790 6 7 2

0.5mg (mg 2mg 3mg 50mg 00mg 4mg mg 0.5mg/g 0.5mg/g mg/g 2mg/g mg mg 00mg 5mg 0mg 0mg 0mg 65mg/g 5mg L- 200mg 4mEq/g 2.5mEq 5mEq 50mg 00mg 05mg 25mg 00mg/g 50mg 0mg 30mg 00mg 250mg 00mg/g L- 265mg 00mg 45mg mg ) 25mg - 986mg/g 00mg/g 00mg/g 0mg 30mg 500mg/g 500mg/g 250mg 500mg 250mg 50mg 50mg 00mg 20mg/g 50mg 250mg 83.3mg/g 50mg 00mg/g 500mg 25mg 0mg 50mg 59mg/g 0mg 20mg 270mg/g 50mg/g 60mg/g 3.5mg/g 45mg/g 25mg/g 0mg/g 2.25mg/g 35mg 7530mg 6.75mg 30mg 6mg 800mg/g 20mg/g

0.5 mg 900 ml 2 50 5 20 ml 0.45 m 0 ml (V) 4 0.022 g 00 ml 5 ml 00 ml 5 ml 00 ml 4 ml / 4 : 7 252 ml 0 mol/l 2 ml 00 L B 2 B 2 A T A S 5 85 % C 20 H 32 O 5 % T 9 W S A AS C 4 W S : C : C 20 H 32 O 5 : : 280 nm : 4.6 mm 5 cm 5 m : 40 : // (3250 : 750 : 3) : B 2 B 2 9 : 00 L B 2 B 2 8000.5 : 00 L 6 B 2 B 2 2.0

C 20 H 32 O 5 : 352.47 (Z) - 7 - [(R, 2R, 3R) - 3 - - 2 - [(E) - (3S) - 3 - - - ] - 5 - ] - 5-3 (V) 4 ( ) 20000 ml 7 000.5 ml 0.5 ml 5 0 ml 7 000.5 ml 0 ml 275 280 nm ( 2 ) 20 745 cm 75 cm 60 cm 075 cm 970 cm 20 D : 79 85 ( 0. g (99.5)5 ml00 mm) 5 mg 5 ml ml 200 ml 0 L // / 00 : : 4 : 2 0 cm 4-50 3.0 0.5 g (V)4 99.0 0.05 g (99.5) 5 ml 0.04 mol/l : 0.04 mol/l ml 4.099 mg C 20 H 32 O 5 0.04 mol/l 000 ml NaOH : 39.997.5999 g 0.2 mol/l 5

mg 900mL 2 50 30 20mL 0.45m 0mL 05 4 0.02g 200mL ml 00mL 50L A T A S 30 80% C 22 H 3 NO 3 HC % AT WS AS C 9 2 W S C C 22 H 3 NO 3 HC 225nm 4.6mm 5cm 5m 25 500 0 ph 3.5 400mL 600mL 8 50L 2500 2.0 50L 6 2.0 C 22 H 3 NO 3 HC 99.0

2mg 900mL 2 50 30 20mL 0.45 m 0mL 05 4 0.02g 200mL 2mL 00mL 50L A T A S 30 80% C 22 H 3 NO 3 HC % AT WS AS C 9 2 W S C C 22 H 3 NO 3 HC 225nm 4.6mm 5cm 5m 25 500 0 ph 3.5 400mL 600mL 8 50L 2500 2.0 50L 6 2.0 C 22 H 3 NO 3 HC 99.0

3mg 900mL 2 50 30 20mL 0.45 m 0mL 05 4 0.02g 200mL 3mL 00mL 50L A T A S 30 75% C 22 H 3 NO 3 HC % AT WS AS C 9 2 W S C C 22 H 3 NO 3 HC 225nm 4.6mm 5cm 5m 25 500 0 ph 3.5 400mL 600mL 8 50L 2500 2.0 50L 6 2.0 C 22 H 3 NO 3 HC 99.0

50mg ph6.8 2900mL 2 50 30 20mL 0.45m 0mL 80 2 0.025g ph6.8 2 00mL 0mL ph6.8 2 50mL ph6.8 2 26nm A T(n) A S 30 85% C 2 H 29 N 3 O % AT S = W 80 AS C W S C C 2 H 29 N 3 O ( ) (C 2 H 29 N 3 O)99.0.

00mg ph6.8 2900mL 2 50 30 20mL 0.45m 0mL 5mL ph6.8 2 0mL 80 2 0.025g ph6.8 2 00mL 0mL ph6.8 2 50mL ph6.8 2 26nm A T(n) A S 30 85% C 2 H 29 N 3 O % AT S = W 360 AS C W S C C 2 H 29 N 3 O ( ) (C 2 H 29 N 3 O)99.0.

4mg 900mL 2 50 30 20mL 0.5µm 0mL 052 0.022g 95 00mL 5mL 50mL 5mL 25mL 20µL A T S 0.2 A T2 S2 30 80 C 9 H 8 ClFN 2 O 3 % A T + A T 2 = W s 8 A S + A S 2 C S C 9 H 8 ClFN 2 O 3 250nm 4.6mm 5cm 5µm 40 / 50mMpH4.05050 8 20µL 0.2 2 2.0 20µL 6 0.2 2.0% C 9 H 8 ClFN 2 O 3 99.0% 0.05mol/LpH4.0 003.0 000mL 3.4g 500mL ph4.0

N- mg 900 ml 2 50 45 20 ml 0.45m 0 ml N- 05 3 0.028 g 250 ml 2 ml 200 ml 30L N- A T A S 45 85 % N- (C 9 H 27 NO 8 S) () AT 8 = WS A C 5 S Ws N- C N- (C 9 H 27 NO 8 S) 20 nm 4.6 mm 5 cm 5 m 40 0.2 mol/l 7:3 N- 0 30 L N- 3000 2.0 30 L 6 N- 2.0 % N- N- (C 9 H 27 NO 8 S)99.0 %

0.5mg/g 2 900mL 2 50 5 30mL 0.45 m 20mL 5mL ml 05 4 0.022g 00mL 5L 00L 5 50 5mL ml 00 A T S 5 85 C 29 H 33 ClN 2 O 2 HCl W S A T W A S C 9 2 W S W g Cg C 29 H 33 ClN 2 O 2 HCl 24nm 4.6m 5cm 5m 40 3.0 0)0 550 450 5 00L 5000 2.0 00L 6 2.5 C 2 H 5 3 NHCl 2 20mL 20mL 97.0 0.3 50mL 2 20 ml 0.mol/L

0.mol/L ml37.65 C 2 H 5 3 NHCl

0.5mg/g 2.0 g ph4.0 0.05mol/L 900mL 2 50 5 20mL 0.45µm 0mL 054 0.022g 00mL 5mL ph4.0 0.05mol/L 00mL 5mL ph4.0 0.05mol/L 50mL 00µL A T A S 5 85 C 29 H 33 ClN 2 O 2 HCl () Ws A 9 T W A C 2 W S W T C T S (mg) g g C 29 H 33 ClN 2 O 2 HCl 24nm 4.6mm 5cm 5µm 40 3.0g 540mL 0 0mL 450mL 6 00µL 5000 2.0 00µL 6 2.5% 0.05mol/L ph4.0 00 3.0g 000mL 3.4g 500mL ph4.0 C 2 H 5 3 NH Cl 2g 20mL g (95) 20mL

97.0 0.3g 50mL 2% 20% ml 0.mol/L 0.mol/L ml 37.65mg C 2 H 5 3 NH Cl

mg/g.0 g ph4.0 0.05mol/L 900mL 2 50 5 20mL 0.45µm 0mL 054 0.022g 00mL 5mL ph4.0 0.05mol/L 00mL 5mL ph4.0 0.05mol/L 50mL 00µL A T A S 5 75 C 29 H 33 ClN 2 O 2 HCl () Ws A 9 T W A C 2 T S W S (mg) W T g C g C 29 H 33 ClN 2 O 2 HCl 24nm 4.6mm 5cm 5µm 40 3.0g 540mL 0 0mL 450mL 6 00µL 5000 2.0 00µL 6 2.5% 0.05mol/L ph4.0 00 3.0g 000mL 3.4g 500mL ph4.0 C 2 H 5 3 NH Cl 2g 20mL g

(95) 20mL 97.0 0.3g 50mL 2% 20% ml 0.mol/L 0.mol/L ml 37.65mg C 2 H 5 3 NH Cl

2mg/g 0.5g ph4.0 0.05mol/L 900mL 2 50 30 20mL 0.45m 0mL 054 0.022g 00mL 5mL ph4.0 0.05mol/L 00mL 5mL ph4.0 0.05mol/L 50mL 00 L A T A S 30 80 C 29 H 33 ClN 2 O 2 HCl W 9 = S A T W A C 2 T S W S W T g C g C 29 H 33 ClN 2 O 2 HCl 24nm 4.6mm 5cm 5m 40 3.0g 540mL 0 0mL 450mL 6 00L 5000 2.0 00L 6 2.5 0.05mol/L ph4.0 00 3.0g 000mL 3.4g 500mL ph4.0 C 2 H 5 3 NHCl 2g 20mL g

9520mL 97.0 0.3g 50mL 2 20 ml 0.mol/L 0.mol/L ml 37.65mg C 2 H 5 3 NHCl

mg 900mL 2 50 30 20mL 0.45µm 0mL 5mL 2mL 054 0.022g 00mL 5mL 00mL 5mL 50mL 5mL 2mL 00µL A T A S 30 80 C 29 H 33 ClN 2 O 2 HCl () A 9 W S T AS C 2 W S C (mg) C 29 H 33 ClN 2 O 2 HCl 24nm 4.6mm 5cm 5µm 40 3.0g 540mL 0 0mL 450mL 6 00µL 5000 2.0 00µL 6 2.5% C 2 H 5 3 NHCl 2g 20mL g 20mL 97.0 0.3g 50mL 2% 20% ml 0.mol/L

0.mol/L ml 37.65mg C 2 H 5 3 NHCl

mg 900mL 2 50 45 20mL 0.45µm 0mL 5mL 2mL 054 0.022g 00mL 5mL 00mL 5mL 50mL 5mL 2mL 00µL A T A S 45 75 C 29 H 33 ClN 2 O 2 HCl () A 9 W S T AS C 2 W S C (mg) C 29 H 33 ClN 2 O 2 HCl 24nm 4.6mm 5cm 5µm 40 3.0g 540mL 0 0mL 450mL 6 00µL 5000 2.0 00µL 6 2.5% C 2 H 5 3 NH Cl 2g 20mL g (95) 20mL 97.0 0.3g 50mL 2% 20% ml 0.mol/L

0.mol/L ml 37.65mg C 2 H 5 3 NH Cl

00mg 5 0mg 0 900mL 2 50 90 20mL 0.45m 0mL A A 5mL 50mL B 90 05 4 0.028g 00mL 2mL 50mL B 273nm AT AS 330nm AT2 AS2 90 80 C0H4N4O4 (%) AT AT2 360 WS AS AS2 C WS C C0H4N4O4 05 4 05 3 05 3 0.055g 05 4 0.028g 20mL 00mL 2mL 00mL A 20L ATAT2AT3 AT4 ASAS2AS3 AS4 90 80 C0H5NOHCl (%)

AT 8 WS AS C WS C C0H5NOHCl 90 75 C20H2NO4HCl (%) AT28 WS2 AS2 C WS2 C C20H2NO4HCl 90 75 C22H23NO7 (%) AT38 WS3 AS3 C WS3 C C22H23NO7 90 75 C7H2NOHCl (%) AT4 8 WS4 AS4 C WS4 C C7H2NOHCl 25nm 4.6mm 25cm 5m InertsilODS-2 40

- 000 532 454 8 20L 2000 2.0 20L 6.5% C20H2NO4HCl99.0 C22H23NO799.0 C7H2NOHCl99.0

65g 0.25g ph4.00.05mol/l 900mL 50 60 20mL 0.45m 0mL ml ph4.00.05mol/l 25mL 05 0.023g 50mL ml ph4.00.05mol/l 00mL ph4.0 0.05mol/L 249nm A T A S 60 70 C 7 H 20 N 2 SC 4 H 0 O 4 Ws AT = 80 WT As C Ws W T g C g C 7 H 20 N 2 SC 4 H 0 O 4 C 7 H 20 N 2 SC 4 H 0 O 4 526.65N,N- --[( -0- ) ] 96 00000 253256nm 28729nm 3230cm - 654 cm - 602 cm - 460 cm - 360 cm - 050 cm - 835 cm - 75 cm - 0.0g NN ml 50mL ml 200mL 0L 254nm 4.6mm 25cm m

40.56g 900mL 0.05mol/L ph 6.8 000mL 400mL 600mL ml 25mL 0L 0~30 mg ml 250mL 0L 0 0L 2.0% 0.5 0.5g05 99.0.0g 00 50mL 0.mol/L 0.mol/L ml52.67c 7 H 20 N 2 SC 4 H 0 O 4 0.05mol/LpH4.0 003.0 000mL 3.4 500mL ph4.0

5mg 7000900mL 2 00 90 20mL 0.45m 0mL 05 3 0.028g 50mL 2mL 7000 200mL 50L A T A S 90 80 C 22 H 32 O 3 AT = WS 8 AS C W S C C 22 H 32 O 3 244 nm 4.6mm 5cm 5m 25 4 6 50L 2000 2.0 50L 6 2.0% C 22 H 32 O 3 99.0%

L- 200mg ph6.8 900 ml 2 50 30 20mL 0.5 m 0mL 4mL 20mL 80 4 0.028g 0.molL 5 ml 00mL 5mL 20mL 4mL 20mL 50L A T A S 30 80 L C 8 H 2 CaN 2 O 8 % AT = WS 225 3. 040 AS C W S C L C 8 H 2 CaN 2 O 8 4.6 mm 5 cm 6m 40 4.0mmol 8 50L 000 2.0 50L 6 2.0 CaCO 3 99.0 ph6.8 2.g 000mL ph 6.8 4.0mmolL 0.5molL 8mL 000mL

4mEq/g C 6 H KO 7 937mg 900mL 2 50 5mL 0.45m 5mL 30 2 0.03g 00mL 20L A T A S 5 80% C 6 H KO 7 % A T W S 9003.42 A S C W S C C 6 H KO 7 5mm 5cm 0m 40 340 6 20L 500 20L 6 2.5%

2.5mEq 900mL 2 75 5mL 0.45m 5mL 30 2 0.09g 00mL 20L A T A S 30 80 C 6 H KO 7 % A T = W S 9003.42 A S C W S C C 6 H KO 7 5mm 5cm 0m 40 340 6 20L 500 20L 6 2.5%

5mEq 900mL 75 5mL 0.45m 5mL 30 2 0.09g 50mL 20L 60 80% 9006.284 6 7 5 5 0 40 340 6 20 500 20 6 2.5

50mg 900mL 2 50 30 60 80 20mL 20mL 0.45µm 0mL 0.027g 00mL 3mL 0.mL 285mL 25mL 535nm A T(n) A S 30 60 80 545 3565 85 Fe 23 A n T(n) T(i) = S + A W 900 0.2009 S i S 45 A = A C W S C Fe FeSO 4 7H 2 O 99.002.0

00mg 900mL 2 50 90 20 5 20mL 370.5 20mL 0.45µm 0mL 5mL 0mL 0.027g 00mL 3mL 0.mL (28) 5mL 25mL 535nm A T(n) A S 90 20 5 2050%3060% 80% n (Fe) (%) (n23) n A T(n) T(i) = S + A W 800 0.2009 S i S45 A = A C W S (mg) C (Fe) (mg) ( ) FeSO 4 7H 2 O 99.002.0

05mg 900mL 2 50 2 6 20mL 370.5 20mL 0.45m 0mL 2mL 2mL 0.027g 00mL 3mL 0.mL 285mL 25mL 535nm A T(n) A S 2 6 545 3565 75 Fe n=23 n AT(n) A (i) = W s 45 + T 40 0. 2009 A A S i= S C W S C Fe 0.2009 FeSO 4 7H 2 O 99.002.0

25, 900 ml,, 50. 60, 20 ml, 0. 45 m. 0 ml, ml, ml,. 05, 0.025 g,, 50 ml. ml, 00 ml,.,, 228 nm A T A S. 60 75. C 9 H 2 NSHCl AT W S 08 AS C WS (mg) C C 9 H 2 NSHCl (mg),, C 9 H 2 NSHCl99.0 %,. 5,, 05 4.. 224 225 0.20 % g05 4

00mg/g 0.5g 900mL 2 50 5 20mL 0.45m 0mL 05 3 0.028g 50mL 5mL 50mL 20L AT AS 5 80% C2H30FN3O22HCl (%) WS AT 900 WT AS C 5 WS WT g Cg C2H30FN3O22HCl 248nm 4.6mm 5cm 5m 40 0.05mol/L 73 5 20L 2000 2.0 20L 6.5 C2H30FN3O22HCl99.0

50mg 900mL 2 50 30 20mL 0.45m 0mL 05 3 0.028g 50mL 5mL 50mL 20L AT AS 30 85% C2H30FN3O22HCl (%) AT 900 WS AS C 5 WS C C2H30FN3O22HCl 248nm 4.6mm 5cm 5m 40 0.05mol/L 73 5 20L 2000 2.0 20L 6.5 C2H30FN3O22HCl99.0

0 mg 900mL 2 75 5 20 ml 0.45 m 0 ml 65 3 0.028 g 00 ml 2 ml 50 ml 0L AT A S 5 80 C 8 H 20 N 2 HC (%) AT = WS C 36 AS W S (mg) C C 8 H 20 N 2 HC 280nm 4.6mm 5cm 5m 40-5.g 8.5mL 000L 200mL 650L 50mL 5 0 L 3000.5 0 L 6.0 C 8 H 20 N 2 HC 300.83 99.0

30 mg 900mL 2 75 45 20 ml 0.45 m 0 ml 5mL 5mL 65 3 0.028 g 00 ml 2 ml 50 ml 0L AT A S 45 70 C 8 H 20 N 2 HC (%) AT = WS C 08 AS W S (mg) C C 8 H 20 N 2 HC 280nm 4.6mm 5cm 5m 40-5.g 8.5mL 000L 200mL 650L 50mL 5 0 L 3000.5 0 L 6.0 C 8 H 20 N 2 HC 300.83 99.0

00 mg ph.2 900 ml 2 00 20 20 L 0.5m 0 L 2mL ph 4.0 20mL 05 3 0.022g ph 4.0 00 L 5L ph 4.0 00 L 20L A T A S 20 5 C 0 H 7 N 3 O 6 S % AT = WS 450 AS C W S C C 0 H 7 N 3 O 6 S ph 6.8 ph 6.8 900 ml 2 00 90 20 L 0.5m 0 L 2mL ph 4.0 20mL 05 3 0.022g ph 4.0 00 L 5L ph 4.0 00 L 20L A T A S 90 85 C 0 H 7 N 3 O 6 S % AT = WS 450 AS C W S C C 0 H 7 N 3 O 6 S

( 20 nm) 4.6 mm 5 cm 5 m 30 6.8 g - 2.0 g 000 ml ph 3.0 930 ml 70 L 5 20 L 2000 2.0 20 2.0 ph 4.0 5.3 000 L 7. 000 L ph 4.0 ph 6.8 2. 000 L ph 6.8 C 0 H 7 N 3 O 6 S99.0

250mg 900mL 2 50 60 20mL 0.45m 0mL 0.028 00mL 0L 60 75 () 900 mg( ) mg( ) 270nm 4.6mm 5cm 5m. 40 72500 4: 5 0L 2.0 2000 0L 6 2.0

00mg/g 0.5 g ph 6.8 50900 ml 2 50 5 20 ml 0.45 m 0 ml 0.028 g 99.5 50 ml 5 ml ph 6.8 50 ml 0 µl A T A S 5 70 C 23 H 38 O WS AT 80 WT A S C W S W T 00mg/g g C g C 23 H 38 O 20 nm 4.6 mm 5 cm 5 µm 40 87 : 3 8 0 µl.0 0 µl 6.5 % C 23 H 38 O330.55(9E,3E)-6,0,4,8- -5,9,3,7- -2- (5E:5Z) 3:2

78cm - 442cm - 358cm - 58cm - () 0.02 g 4 ml ml 20 ml ml 0 ml 4 µl 3 mm 2 m 2-4977 µm 5 % 20 5 20 2 4 µl 4 8 mm ml ml µl. 4 µl 6 3.0 (2) 0.0 g 2 ml ml 20 ml ml 0 ml 0 µl 73 0 cm n (00) 20 90 20 99.0 % 0.7 g 25 ml 30

0.5mol/L 0 0.5mol/L ml65.28c 23 H 38 O ph 6.8 0.05mol/L 000mL 5.25g 000mL ph 6.8

L- 265L- 00 45mg 900 ml 2 50 30 20 ml 0.45 m 0 ml 2 ml 00 ml 05 3 L- 0.2 g 05 3 L- 0.045 g 05 3 0.02g 200 ml 2 ml 200 ml 0 L L- A Ta A Sa L- A Tb A Sb A Tc A Sc 30 L- L- 80 85 85 L- C 5 H 9 NO 4 ATa = WSa 225 ASa Ca W Sa L- C a L- C 5 H 9 NO 4 L- C 3 H 7 NO 2 ATb = WSb 225 ASb Cb W Sb L- C b L- C 3 H 7 NO 2 C 2 H 5 NO 2 ATc = WSc 225 ASc Cc W Sc C c C 2 H 5 NO 2 340 nm 455 4.6 mm 5 cm 5 m 45 0.5mm 2m.5 g 4.4 g 000 ml

9 300 ml o - 60 mg 0 ml 3-0.2 ml 500 ml 45.4 ml 0.9 ml 0L L- L-.5 0L 6 2.0 L- L- C 5 H 9 NO 4 99.0 L- L- C 3 H 7 NO 2 99.0 C 2 H 5 NO 2 99.0 3- C 3 H 6 O 2 S06.4 2g 20mL 97.0% 3g 00mL 0mL 50mL 0.5mol/L 0.5mol/L ml53.07c 3 H 6 O 2 S

mg 900mL 50 5 20mL 0.45µm 0mL 05 2 0.022g 200mL 2mL 200mL 00 µl A T A S 5 80 C 0 H 5 NHCl AT 9 = Ws As C 2 W S C C 0 H 5 NHCl 257nm 4.6 mm 5 cm 5 µm 40. g 00 750 (209) 000 ml 7 00 µl 4000 2.0 00 µl 6 3.0 % C 0 H 5 NHCl99.0%

25 900mL 50 45 20mL 0.45m 0mL ml 25mL 05 0.028 00mL ml 00mL 29nm A T A S 45 70 C 2 H 2 N 2 O 2 S AT 90 Ws As C Ws C C 2 H 2 N 2 O 2 S C 2 H 2 N 2 O 2 S248.30 3460cm - 3240cm - 63cm - 590cm - 278cm - 05cm - 828cm - 540cm - 7579 0.020g 25ml ml 20ml ml 25ml L 254nm 6mm 5cm m 25.36g 000mL ph 6.5 650mL 350mL

ml 25mL L 030% 0.02g 25mL ml 00mL L L 2.0.0% g05 99.0% 0.25g 0mL 40mL 00mL 5 0.mol/L 0.mol/L ml=2.45c 2 H 2 N 2 O 2 S

986mg/g g 900 ml 2 50 5 20 ml 0.45 m 0 ml - 05 3 0.022 g 20 ml 0L - A T A S 5 85 % - C 6 H 3 NO 2 % WS A T = 4500 WT A C S Ws - W T - g Cg - C 6 H 3 NO 2 20 nm 4.6 mm 5 cm 5m 30 3.33g - 0.63g 000mL ph2.2 750mL 250mL - 9 0L - 3000 2.0 0L 6-2.0% - - C 6 H 3 NO 2 99.0%

00mg/g 0.3 g 900 ml 2 50 5 20 ml 0.5 m 0 ml 5 ml 5 ml 0.028 g 50 ml 2 ml 00 ml 363 nm AT AS 5 85 % C 0 H N 4 NaO 5 S 3H 2 O (%) W = W S T AT 08. 68 AS C WS mg WT g C g C 0 H N 4 NaO 5 S 3H 2 O mg C 0 H N 4 NaO 5 S99.0 %

00mg/g 0.3g 900mL 50 5 20mL 0.5m 0mL 5mL 0mL 0.028g 50mL 2mL 00mL 363nm A T A S 5 85 C 0 H N 4 NaO 5 S3H 2 O WS AT = 08.677 WT AS C W S W T g Cg C 0 H N 4 NaO 5 S3H 2 O C 0 H N 4 NaO 5 S322.2799.0

0mg 900 ml 2 50 5 20 ml 0.5 m 0 ml 0.028 g 50 ml 2 ml 00 ml 363 nm AT AS 5 85 % C 0 H N 4 NaO 5 S 3H 2 O % AT = WS 36.68 AS C WS C C 0 H N 4 NaO 5 S 3H 2 O C 0 H N 4 NaO 5 S99.0 %

30mg 900 ml 2 50 5 20 ml 0.5 m 0 ml 5 ml 5 ml 0.028 g 50 ml 2 ml 00 ml 363 nm AT AS 5 80 % C 0 H N 4 NaO 5 S 3H 2 O % AT = WS 08.68 AS C WS C C 0 H N 4 NaO 5 S 3H 2 O C 0 H N 4 NaO 5 S99.0 %

500mg/g g 900 ml 2 50 5 20 ml 0.45 m 0 ml 05 2 0.028 g 50 ml 0 L A T A S 5 85 % C 8 H 5 NO 2 () WS AT = 800 W A C T S Ws W T 500mg/g (g) C g C 8 H 5 NO 2 220 nm 4.6 mm 5 cm 5m 25.0g 500mL 0mL.4g ph2.5 600mL 400mL 8 0L 4000 2.0 0L 6 3.0%

500mg/g g 900mL 2 50 5 20mL 0.45 m 0mL 05 2 0.028g 50mL 0 L A T A S 5 80% C 8 H 5 NO 2 (%) WS AT = 800 WT AS C Ws : (mg) W T : g : g C 8 H 5 NO 2 (mg) : 220nm 4.6mm 5cm 5m 25.0g 500mL 0mL.4g ph2.5 600mL 400mL 8 0L 4000 2.0 0L 6 3.0 C 8 H 5 NO 2 99.0%

250mg 900 ml 2 50 45 20 ml 0.45m 0 ml 05 2 0.028 g 00 ml 0L A T A S 45 80 % C 8 H 5 NO 2 () AT = WS 900 A C S Ws C C 8 H 5 NO 2 220 nm 4.6 mm 5 cm 5m 25.0g 500mL 0mL.4g ph2.5 600mL 400mL 8 0L 4000 2.0 0L 6 3.0%

500mg 900 ml 2 50 45 20 ml 0.45m 0 ml 5 ml 0 ml 05 2 0.028 g 00 ml 0L A T A S 45 85 % C 8 H 5 NO 2 () AT = WS 800 A C S Ws C C 8 H 5 NO 2 220 nm 4.6 mm 5 cm 5m 25.0g 500mL 0mL.4g ph2.5 600mL 400mL 8 0L 4000 2.0 0L 6 3.0%

250mg 900 ml 2 50 5 20 ml 0.45m 0 ml 05 2 0.028 g 00 ml 0L A T A S 5 80 % C 8 H 5 NO 2 () AT = WS 900 A C S Ws C C 8 H 5 NO 2 220 nm 4.6 mm 5 cm 5m 25.0g 500mL 0mL.4g ph2.5 600mL 400mL 8 0L 4000 2.0 0L 6 3.0%

50mg 900 ml 2 50 30 20mL 0.45m 0mL 5mL 50mL 7g 00mL 5L 50mL 275nm A T A S 30 80% % A T = Ws 900 As C Ws C

50mg 900mL 2 50 30 20mL 0.45m 0mL 4mL 20mL 0.028g 50mL 2mL 00mL 274nm A T A S 30 85 AT = WS 0. 8 AS C Ws C

00mg 900mL 2 50 30 20mL 0.45m 0mL 2mL 20mL 0.028g 50mL 2mL 00mL 274nm A T A S 30 85 AT = WS 0. 36 AS C Ws C

20mg/g g 900mL 2 50 5 20 ml 0.45m 0mL 22 00mL 5mL 50mL 348nm A T A S 5 85 Ws AT = W T A S C 90 W S W T g C g

50 900 ml 2 50 45 20 ml 0.45 µm 0 ml 0 ml 25 ml 22 00 ml 5 ml 50 ml 278 nm T S 45 75 A T W S 225 A S C s mg C H 2 Cl 2 N 2 O 5 980µg

250 900 ml 2 50 45 20 ml 0.45 µm 0 ml 2 ml 25 ml 22 00 ml 5 ml 50 ml 278 nm T S 45 70 A T W S 25 A S C s mg C H 2 Cl 2 N 2 O 5 980µg

784.8mg/g 83.3mg/g Fe0.05 0.6g 900 ml 2 50 90 20mL 0.45 0L 5mL 00mL 2mL mol/l 2mL 02mL,0- ph5.0 0003mL ph5.0 50 ml 50nm ATAS AB 90 80 Fe (%) =WS/WT AT-AB)/AS-AB C900 WS WT g C g Fe) 0.95gW 20mL mol/l 5mL 00mL ml FeW0.863 mg

470.9 50 900L 2 50 45 20L 0.45 0L 5mL 00 L 2L mol/l 2mL 02L,0- ph5.0 0003L ph5.0 50L 50nm ATAS AB 45 75 Fe (%) =WS AT-AB)/AS-AB C900 WS C Fe 0.95gW 20mL mol/l 5mL 00mL ml FeW 0.863mg

9mg/g g ph4.0 0.05mol/L 900mL 2 50 5 20mL 0.5µm 0mL 5mL 0.mol/L 00mL () 4 0.025g 0.mol/L 00mL 2mL ph4.0 0.05mol/L 5mL 0.mol/L 00mL 262nm A T A S 5 85 C 2 H 26 N 2 S 2 WS AT = 360 W A C T S W S W T g Cg C 2 H 26 N 2 S 2 C 2 H 26 N 2 S 2 99.0%

500 mg ph 6.8 2900 ml 2 75 30 20 ml 0.45 µm 0 ml 2 ml ph 6.8 2 00 ml 05 4 22 mg ph 6.8 2 00 ml 5 ml ph 6.8 2 00 ml cm 360 nm A T A S 30 70% C 8 H 4 N 4 O 5 S % A T = WS 2250 A S C W S C C 8 H 4 N 4 O 5 S C 8 H 4 N 4 O 5 S99.0%

25mg 6.8 (2) 900 ml 50 5 20 ml 0.45 m 0 ml 24 35 mg 25 ml 5 ml 50 ml 00 L 5 75 ( 24 40 4 ) AT S W 360 AS C S mg) ( 24 40 4 ) (mg) 220nm 4.6 mm 5cm 5m 40 (2000) 5 00L 3000 2.0 00L 2.0 (C 24 H 40 O 4 ) 99.0 %

0 ph4.00.05moll 900mL 2 50 45 20mL 0.45µm 0mL 05 2 0.022g ph4.00.05moll 00mL 5mL ph4.00.05moll 00mL 249nm A T A S 45 75% C 9 H 24 N 2 SHCl % AT W S 45 AS C W S C C 9 H 24 N 2 SHCl C 9 H 24 N 2 SHCl99.0% 0.05molLpH4.0 (00)3.0g 000mL 3.4g 500mL ph4.0

50 ph4.00.05moll 900mL 2 50 45 20mL 0.45µm 0mL 5mL ph4.00.05moll 25mL 05 2 0.022g ph4.00.05moll 00mL 5mL ph4.00.05moll 00mL 249nm A T A S 45 75% C 9 H 24 N 2 SHCl % AT W S 225 AS C W S C C 9 H 24 N 2 SHCl C 9 H 24 N 2 SHCl99.0% 0.05molLpH4.0 (00)3.0g 000mL 3.4g 500mL ph4.0

59mg/g 0.g ph4.00.05moll 900mL 2 50 20 20mL 0.45µm 0mL 05 2 0.03g 50mL 3mL ph4.00.05moll 00mL ph4.0 0.05molL 293nm A T A S 20 70% C 9 H 24 N 2 SC 4 H 0 O 4 % WS AT 54 WT AS C W S W T g C g C 9 H 24 N 2 SC 4 H 0 O 4 C 9 H 24 N 2 S C 4 H 0 O 4 99.0% 0.05molLpH4.0 (00)3.0g 000mL 3.4g 500mL ph4.0

0mg ph 4.0 0.05 mol/l 900 ml 2 50 30 20 ml 0.45m 0 ml 05 2 0.028 g 50 ml 2 ml ph 4.0 0.05 mol/l 00 ml ph 4.0 0.05 mol/l 285 nm A T A S 30 70% (C 24 H 26 N 2 O 4 ) % A = T Ws 36 A C S Ws (mg) C (C 24 H 26 N 2 O 4 ) (mg) C 24 H 26 N 2 O 4 406.47 -- -4- -3-[[2-o- ] ]-2-20 g 400 ml (G4) 70 2 4 99.5400 ml 4 (G4) 70 8 C 24 H 26 N 2 O 4 406.47 99.0 200000 222 226 nm24 245 nm 284 288 nm 37 32 nm 330 334 nm 2 05 2 3340 cm- 2920cm- 608 cm- 59 cm- 502 cm-454 cm- 257 cm- 224 cm- 0 cm- 65 mg 00 ml ml 0 ml ml 00 ml 20L

3.4 0.02 0. 0.3 240 nm 4.6 mm 5 cm 5m 55 2.72 g 900 ml ph 2.0 000 ml 650 ml 350 ml 4 9 2 ml 20 ml 20L 73 50 mg 0. g 00 ml ml 0 ml ml 00 ml 20L 5 20L 6 2.0 0.5% g05 2 0.0 g 30 ml 0.02 moll 0.02 moll ml 8.29 mg C 24 H 26 N 2 O 4 0.05 mol/l,ph4.0 (00)3.0 g 000 ml, 3.4 g 500 ml,ph4.0

20mg ph 4.0 0.05 mol/l 900 ml 2 75 30 20 ml 0.45m 0 ml 5mL ph 4.0 0.05 mol/l 0mL 05 2 0.028 g 50 ml 2 ml ph 4.0 0.05 mol/l 00 ml ph 4.0 0.05 mol/l 285 nm A T A S 30 80% (C 24 H 26 N 2 O 4 ) % A = T WS 72 A C S Ws (mg) C (C 24 H 26 N 2 O 4 ) (mg) C 24 H 26 N 2 O 4 406.47 -- -4- -3-[[2-o- ] ]-2-20 g 400 ml (G4) 70 2 4 99.5400 ml 4 (G4) 70 8 C 24 H 26 N 2 O 4 406.47 99.0 200000 222 226 nm24 245 nm 284 288 nm 37 32 nm 330 334 nm 2 05 2 3340 cm- 2920cm- 608 cm- 59 cm- 502 cm-454 cm- 257 cm- 224 cm- 0 cm- 65 mg 00 ml ml 0 ml ml 00 ml 20L

270mg/g 50mg/g 60mg/g 3.5mg/g 0.5g 900mL 2 50 5 20mL 0.45µm 0mL 4 30mg 05 2 7mg 20mL 80 4 7mg 20mL 05 3 9mg 00mL 5mL 2mL 50mL 0µL A Ta A Sa A Tb A Sb A Tc A Sc A Td A Sd 5 85% 85% 75% C 7 H 7 NO 2 Sa Ta = W A 450 WT ASa Ca C 8 H 9 NO 2 Sb Tb = W A 450 WT ASb Cb C 8 H 0 N 4 O 2 WSc ATc = 80 WT ASc Cc C 34 H 40 N 4 S 2 C 5 H 2 O 6 WSd ATd = 36 WT ASd Cd

W Sa W Sb W Sc W Sd W T g C a g C 7 H 7 NO 2 C b g C 8 H 9 NO 2 C c g C 8 H 0 N 4 O 2 C d g C 34 H 40 N 4 S 2 C 5 H 2 O 6 250nm 4.6mm 5cm 5µm 40 000mL 0.5mL ml ph2.5 800mL 44mL 80mL 3 0µL 5 3 0µL 6 3.0% C 7 H 7 NO 2 99.0% (C 34 H 40 N 4 S 2 C 5 H 2 O 6 )99.0% CH 3 (CH 2 ) 7 NH 2 29.24 n 20 D.426.432-80 -

45mg/g 25mg/g 0mg/g 2.25mg/g g 900mL 2 50 5 90 20mL 370.5 20mL 0.45µm 5 90 0mL 2 4 30mg 05 2 7mg 20mL 80 4 7mg 20mL 05 3 9mg 00mL 0mL 4mL 300mL 2 30µL A T()a A S()a A T()b A S()b A T()c A S()c A T(n)d A S(n)d 5 85% 85% 90 75% C 7 H 7 NO 2 Sa T()a = W A 50 WT AS()a Ca C 8 H 9 NO 2 Sb T()b = W A 50 WT AS()b Cb C 8 H 0 N 4 O 2 WSc AT()c = 60 WT AS()c Cc C 34 H 40 N 4 S 2 C 5 H 2 O 6 WSd AT(2)d AT()d = + 2 WT AS(2)d AS()d 45 Cd W Sa W Sb W Sc W Sd W T - 8 -

g C a g C 7 H 7 NO 2 C b g C 8 H 9 NO 2 C c g C 8 H 0 N 4 O 2 C d g C 34 H 40 N 4 S 2 C 5 H 2 O 6 250nm 4.6mm 5cm 5µm 40 000mL 0.5mL ml ph2.5 800mL 44mL 80mL 3 30µL 5 3 30µL 6 3.0% C 7 H 7 NO 2 99.0% (C 34 H 40 N 4 S 2 C 5 H 2 O 6 99.0% CH 3 (CH 2 ) 7 NH 2 29.24 n 20 D.426.432-82 -

35 75 30 6.75 900mL 2 50 45 20mL 0.45m 0mL 4 0.030g 05 0.07g 20mL 80 4 0.07g 20mL 05 3 0.09g 00mL 5mL 2mL 50mL 0L A Ta A Sa A Tb A Sb A Tc A Sc A Td A Sd 45 75 85 85 75 A = Ta Ws 450 A C Sa A = Tb Ws 450 A C Sb A = Tc Ws 80 A C Sc - 83 -

A = Td Ws 36 A C Sd 250 nm 4.6 mm 5 cm 5m 40 000mL 0.5mL ml ph 2.5 800mL 44mL 80mL 3 0L 5 3 0L 6 3.0 99.0 ) 99.0 ( - 84 -

30 6mg 900mL 2 50 45 20 ml 0.45m 0mL 5mL 0.mol/L 0 ml. 4 0.033g 0.mol/L 00 ml 0.033g 0.mol/L 50mL, 0 ml 0.mol/L 00mL 0 ml 0.mol/L 200mL 0L AT AS BT BS 45 85 80. WSATASCA90 WS CA C6H6N2O (mg) VSBTBSCB8 VS CB C20H2NO4HCl (mg) ( ) ( ) 250nm 4.6mm 5 5 000mL 0.85 ph2.5 2 0L 4-85 -

6 3.0% 45 00 95 45 00 5 85 95 5 80. - 86 -

20mg/g 800mg/g 0.5 900mL 50 5 90 20mL 0.45m 0mL 5 80 0.022 g 200mL ml 20mL 20L T S 8 0 4 2 W S A T = 45 W A C T S g 8 0 4 2 273nm 4.6mm 5cm m mm m 40 000 7 20L 3000.5 20L.0% 5 90 ml 20mL 05 0.022 g 50mL ml - 87 -

20mL 20L T5 T90 S 28 38 2 6 W A A S T90 T5 = 880 + 20 2 W T A S A S C g 28 38 2 6 280nm 4.6mm 5cm m mm m 40 20L 3000.5 20L.0% - 88 -

0.5mg E2 L280 2F06A 2mg 2 3E9A 3mg 3 3C056B 50mg M042B 00mg N086B 4mg 04002 N- mg KX89 0.5mg/g 20 0.5mg/g 022FBA mg/g 04ABD 2mg/g 0.2% 62092 mg GA mg 025BBC 00mg ZB5S 5mg 0mg 0mg 0mg 65mg/g J493 5mg 2400 200mg Y266 4mEq/g K2045 2.5mEq G2005 5mEq E2005 50mg 20060 00mg R283 05mg 375435 25mg F4270 00mg/g 50mg 27C25S 28B42S

0mg 29BW 30mg 23C24 00mg 00 24003 250 70603 00mg/g 0% 2XA30K - 265mg 66027A - 00mg 45mg 47 25mg 25 Y035-986mg/g 98.6% MMBZH67 00mg/g (AC-7) 0% 28006 00mg/g 3E4B2 0mg (AC-7) 0mg 26336 30mg (AC-7) 30mg 33033 500mg/g 50% PUAAA08 500mg/g 30 250mg 250mg PVAAA68 250mg 500mg 500mg PXAZF29 PYAAA02 50 46-50 35800A 00mg 25807A 20mg/g 992-50mg 50 NW037 83.3mg/g 250mg 250 NE092 25B58S 50mg 50mg 26B5S 00mg/g 2040 500 504798 25mg 3630 0mg 0 Y239 50mg 50 Y420 59mg/g 0% Y4 0mg 0 LJAZL3 20mg 20mg LKAZK64 270mg/g PL 3029 50mg/g

60mg/g 3.5mg/g 45mg/g PL 300 25mg/g 0mg/g 2.25mg/g 35mg HBIE 75mg 30 6.75mg 30mg A M608 6mg 800mg/g AP2 CC53 20mg/g

(ph) (rpm) 0.5mg.2, 4.0, 6.8 50 3907A mg.2, 4.0, 6.8 50 3908B 2mg.2, 4.0, 6.8 50 3908C 3mg.2, 4.0, 6.8 50 3908D 50mg 6.8.2, 4.0, 50 403A 00mg 6.8.2, 4.0, 50 403B 4mg.2, 4.0, 6.8 50 42B N- mg.2, 4.0, 6.8* 50 4409A 0.5mg/g.2, 4.0, 6.8 50 4709A 0.5mg/g 4.0.2, 6.8, 50 4709B mg/g 4.0.2, 6.8, 50 4709C 2mg/g 4.0.2, 6.8, 50 4709D mg.2, 4.0, 6.8 50 4709E mg.2, 4.0, 6.8 50 4709F 00mg.2 4.0, 6.8, 50 484A 5mg 0mg 0mg 0mg 65mg/g 4.0.2, 6.8, 50 492A 5mg.2, 4.0, 6.8 00 497A 0.7w/v% L- 200mg 2 6.8*.2, 4.0, 50 498A 4mEq/g.2, 4.0, 6.8* 50 4920A 2.5mEq.2, 4.0, 6.8* 75 4920C 5mEq.2, 4.0, 6.8* 75 4920D 50mg 2.2, 4.0, 6.8* 50 492A 00mg 2.2, 4.0, 6.8* 50 492B 05mg 2.2, 4.0, 6.8* 50 492C 25mg.2, 4.0, 6.8 50 4928A 00mg/g.2, 4.0, 6.8 50 4930A 50mg.2, 4.0, 6.8 50 4930B 0mg.2, 4.0, 6.8 75 493A 30mg.2, 4.0, 6.8 75 493B 00mg. 2, 6.0, 00 4942A 6.8* 250mg.2, 4.0, 6.8 50 4948A

00mg/g 6.8.2, 4.0, 50 4952A 2.0w/v% L- 265mg.2, 4.0, 6.8 50 4954A L- 00mg 45mg mg.2, 4.0, 6.8 50 50A 25mg.2, 4.0, 6.8 50 502A - 986mg/g.2, 4.0, 6.8 50 503A 00mg/g.2, 4.0, 6.8 50 504A 00mg/g.2, 4.0, 6.8 50 504B 0mg.2, 4.0, 6.8 50 504C 30mg.2, 4.0, 6.8 50 504D 500mg/g.2, 4.0, 6.8 50 505A 500mg/g.2, 4.0, 6.8 50 505B 250mg.2, 4.0, 6.8 50 505C 500mg.2, 4.0, 6.8 50 505D 250mg.2, 4.0, 6.8 50 505E 50mg.2, 4.0, 6.8 50 507A 50mg.2, 4.0, 6.8 50 508A 00mg.2, 4.0, 6.8 50 508B 20mg/g.2, 4.0, 6.8 50 509A 50mg.2, 4.0, 6.8 50 50A 250mg.2, 4.0, 6.8 50 50B 83.3mg/g.2, 4.0, 6.8 5053A 50mg.2, 4.0, 6.8 50 53B 00mg/g 4.0.2, 6.8, 50 54A 500mg 6.8.2, 4.0, 75 55A 25mg 6.8.2, 4.0, 50 56B 0mg 4.0.2, 6.8, 50 59A 50mg 4.0.2, 6.8, 50 59B 59mg/g 4.0.2, 6.8, 50 520A 0mg 4.0.2, 6.8, 50 52A 20mg 4.0.2, 6.8, 75 52B 270mg/g.2 4.0, 6.8, 50 522A 50mg/g 60mg/g 3.5mg/g 45mg/g.2 4.0, 6.8, 50 522B 25mg/g 0mg/g 2.25mg/g 35mg.2 4.0, 6.8, 50 522C

75 30 6.75mg 30mg.2, 4.0, 6.8 50 523A 6mg 800mg/g.2, 4.0, 6.8 50 525A 20mg/g 3.0w/v% 900mL ph.2 ph4.0 0.05mol/L ph6.8 ph6.8* McIlvaine 0.05mol/L 0.025mol/L ph6.8 2 ph6.8* 2.g 000mL ph6.8 McIlvaine 0.05mol/L 0.025mol/L ph 0 7 5 595

6 4 20 6 4 2 042007 mg 00µ L 00µ L 6 2.5% 2.5% 00mg 6.8 6.8 2

0.mol/L ml 37.65mg C 2 H 5 3 NH Cl

6 5 6 4 2 042007 250mg 0.028mg 28mg ph 6.8 6.8

250mg 900mL 2 50 60 20mL 0.45m 0mL 28 00mL 0L 60 75 () 900 mg( ) mg( ) 270nm 4.6mm 5cm 5m. 40 72500 4: 5 0L 2.0 2000 0L 6 2.0

75 30 6.75mg 30mg.2, 4.0, 6.8 50 523A 6mg 800mg/g.2, 4.0, 6.8* 50 525A 20mg/g 3.0w/v% 900mL ph.2 ph4.0 0.05mol/L ph6.8 ph6.8* McIlvaine 0.05mol/L 0.025mol/L ph6.8 2 ph6.8* 2.g 000mL ph6.8 McIlvaine 0.05mol/L 0.025mol/L ph 0 7 5 595

6 5 26 6 4 2 042007 mg AT WS AS C 9 2 WS WS mg mg C C 22H3NO3 HC mg A A T S 9 2

2mg AT WS AS C 9 2 WS WS mg mg C C 22H3NO3 HC mg A A T S 9 2 3mg AT WS AS C 9 2 WS WS mg mg C C 22H3NO3 HC mg A A T S 9 2

mg 900mL 2 50 30 20mL 0.45m 0mL 05 4 0.02g 200mL ml 00mL 50L A T A S 30 80% C 22 H 3 NO 3 HC % WS A A T S 9 2 W S 225nm 4.6mm 5cm 5m 25 500 0 ph 3.5 400mL 600mL 8 50L 2500 2.0 50L 6 2.0 C 22 H 3 NO 3 HC 99.0

2mg 900mL 2 50 30 20mL 0.45 m 0mL 05 4 0.02g 200mL 2mL 00mL 50L A T A S 30 80% C 22 H 3 NO 3 HC % WS W S A A T S 9 2 225nm 4.6mm 5cm 5m 25 500 0 ph 3.5 400mL 600mL 8 50L 2500 2.0 50L 6 2.0 C 22 H 3 NO 3 HC 99.0

3mg 900mL 2 50 30 20mL 0.45 m 0mL 05 4 0.02g 200mL 3mL 00mL 50L A T A S 30 75% C 22 H 3 NO 3 HC % WS W S A A T S 9 2 225nm 4.6mm 5cm 5m 25 500 0 ph 3.5 400mL 600mL 8 50L 2500 2.0 50L 6 2.0 C 22 H 3 NO 3 HC 99.0