TEM with CCD Ver. 5.1 18 10 25 6 50 FILAMENT OFF 80 V () FILAMENT 2 25 FILAMENT OFF FILAMENT 1 min 2-5 L TEM(&CCD) FILAMENT ON CCD( ) - 1 -
3 TEM 3 CCD 3 4 5 6 7 (CCD ) 7 CCD 7 10 10 11 CCD &TEM 11-2 -
1. p3 return PAGE3 ( ) 2. 3. PAGE 3 P1: 28 2: 28 3: 28 4: 5: 50 150 1 2 TEM 1. () 2. ( ) 3. >htset_80 return ok htset[80] 4. HT () 80 kv COND. STIG SPOT SIZE BEAM CURRENT 40 BIOS OBJ. STIG 5. >load_ht return >run return start ht?(kv) 80 return stop ht?(kv) 200 return ht step?(kv 10) 1 return time limit?(min) 120 return HT ACCEL VOLTAGE 2 200 kv BRIGHTNESS FILAMENT : : : BRIT. TILT SHIFT X DEF X CCD 6. CCD CCD ON cool / off / warm OFF COOL 25 o C 30 7. Auto - 3 -
Open Auto 8. Heat Sink 20 50 cool Reset TEM 15 cm 9. (1kV/s ) DOWN BEAM CURRENT DOWN 10. 2 (TEM ) 1 2 11. 1 (!!) 2 2 1!!!! 12. AIR PUMP ( ) 13. 1 14. p3 return PAGE3 ( ) 15. 2 16. P4 32 ( 5 ) 17. 15 1 cm 15 10 cm P4 2 16 P1: 28 2: 28 3: 28 4: 35 5: 50 150 2-4 -
p1 p5 18. FILAMENT FILAMENT ON 4 BEAM CURRENT +1, 2, 3 ( 6) BIOS BIOS ) (kv) BEAM CURRENT 80 40 100 50 120 60 160 80 200 100 * FILAMENT * BIAS * LOW MAG GUN+SHIFT X or Y * MAG1 SELECTOR FOCUS SHIFT Y DEF Y MAG2 LOW MAG WOBBLER HT IMAGE X or Y : : DIFF SCREEN SHUTTER PHOTO SHIFT X or Y GUN 1. 20 K (MAG2 ) FOCUS PAGE1 DV 0 2. Z DEF X or Y 3. GUN DEF x DEF y SHIFT x SHIFT y - 5 -
CRT 16 4. BRIGHTNESS (X and Y ) 5. SpotSize 1 BRIGHTNESS 6. GUN SHIFT x SHIFT y() Y X 7. SpotSize 5 BRIGHTNESS 8. BRIGHT-SHIFT x SHIFT y () 9. 5 8 10. BRIGHTNESS COND. STIGM. DEF x DEF y 11. SpotSize 1 1. Mag1 LOW MAG ( ) 2. WOBBLER-IMAGE X IMAGE Y FOCUS ( FOCUS ) 3. WOBBLER-HT BRIGHT TILT DEF x DEF y WOBBLER-HT OFF (2-3 ) 4. DIFF 5. 0 1 4 (3 ) 6. 7. MAG1-6 -
1. 5 2. OBJ STGM DEF x DEF y 10 3. over under TEM 4. WOBBLER-STIGM BRIGHTNESS SHIFT (CCD ) 1. 2. PHOTO AUTO 3. 2s 4s 4. 5. PHOTO PHOTO 6. PHOTO ( ) 7. CCD 1. ON OFF COOL (TEM ) 2. 30 25.7 3. CCD TEM 10 50 K CCD 5000 4. Gatan Digital Micrograph 5. Image info Global info Specimen, operator CameraView(search) 6. CameraView search Autoexplosure - 7 -
Focus loupe Auto Survey Camera inserted 7. TEM SCREEN 8. CameraView Start camera 9. Yes 10. magnification voltage TEM mode imaging 11. OK 12. value 30000 ( 10000) Brightness start view 13. stopview 14. Advanced setting Process: Gain Normalized Bining Setup: Search/Focus Search 2 Focus Bining 1 CameraView(focus) 15. CameraView focus search 16. Focus loupe CameraAquire 17. Binning 1 AdvancedSetting 18. Start Acquire 19. magnification voltage TEM mode imaging 20. OK - 8 -
CCD 1. Display control 2. Edit menu Data Edit Add scale bar 3. tool 4. standard tool 5. Target Page Save 10 Voltage, Magnification 6. File menu Save As Gatan 16 MB/ CD-R User Data 7. TIFF, JPEG File menu Save Display As TEM CCD 1. camera inserted 2. TEM Screen 3. 20k FILAMENT FILAMENT ON - 9 -
1. CCD Camera Insert 2. SCREEN 3. BEAM CURRENT FILAMENT FILAMENT ON FILAMENT 4. 0 5. 2 6. 10cm 15 1cm 15() TEM 7. 8. PUMP AIR 9. 10. 11. AIR PUMP ( ) 12. 1 13. p3 return PAGE3 ( ) 14. P4 32 ( 5 ) 15. 2 15 1 cm 15 10 cm 16. FILAMENT ON FILAMENT ON 17. 1. FILAMENT BEAM CURRENT 2. FILAMENT ON ()FILAMENT OFF 3. 4. 5. CAMERA 90 6. ( ) ( ) 7. CAMERA 8. CAMERA - 10 -
9. 90 10. 50 >fun return >50 return 1. 2. 3. 4. 5. 50 6. TEM 7. EVAC CCD &TEM 1. CCD Camera Insert 2. SCREEN 3. Gatan Digital Micrograph 4. CCD cool off TEM OFF 5. CCD FIRST LIGHT OFF 6. OFF ON 7. PAGE P3 28 ( ) 11. FILAMENT BEAM CURRENT 12. FILAMENT ON ()FILAMENT OFF 8. 9. 2 10. 1cm 10 10cm 15( ) TEM - 11 -
11. N 2 12. PUMP AIR 13. 14. 15. Accelerator voltage () down BEAM CURRENT down 80kV () HT ( )OFF 16. ACD () 17. 18. 50 3597 keitatcm@mbox.nc.kyushu-u.ac.jp - 12 -