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Image Windows Preferences Edit, Preferences MetaMorph, MetaVue Image Windows Preferences Edit, Preferences Image Windows Preferences 1. Windows Image Placement: Acquire Overlay at Top Left Corner: 1 Acquire 2 Acquire Molecular Devices Japan KK/ Imaging Team (1/12)

Cascade from Top Left Corner: Cascade from Current Image: Set Anchor Default Image Zoom: Acquire Time Lapse Normal Size 100% Default Palette Size: 8 Auto Enhances 64 Molecular Devices Japan KK/ Imaging Team (2/12)

Default LUT: LUT Set by Wavelength Copy annotation when duplicating: Edit, Duplicate anotation Start recording command history when MetaMorph starts: MetaMorph (Command) History Window Window, History Window MetaMorph Window Molecular Devices Japan KK/ Imaging Team (3/12)

2. Processing Enable automatic annotations: (Anotation) Redraw during iterations: Interpolate when Duplicating with Zoom: Duplicate with Zoom(Edit, Duplicate) 100% Movie Preference: Plays movies slower but uses less memory: Movie Stack Plays movies faster but uses more memory: Movie Stack temp Color Model used to display pixel values: RGB, HIS, HSL Display, Color Separate Enable CPU usage compatibility mode: MetaMorph CPU 100% Windows Molecular Devices Japan KK/ Imaging Team (4/12)

3. Open Open Previous/Next to: File, Open Special Open Previous Open Next 1 (Same Windows) (New Window) Open Select Plane dialog after opening a stack: STK Build Stack Stack, Select Plane Molecular Devices Japan KK/ Imaging Team (5/12)

4. Save Query for Close Options on Close All: Close All Close All Options: Close All Query for Close Options on Close All Close All (Close without saving open images) 1 (Exit and query each image for saving) Close All Images (Query all images at once, then close all) Query for Exit for Options on Exit: File, Exit Close All Exit Options: Query for Exit for Options on Exit Exit (Exit without saving open images) 1 (Exit and query each image for saving) Close All Images (Query all images at once, then exit) Molecular Devices Japan KK/ Imaging Team (6/12)

Save Thumbnails for Multi-Dimensional Applications: Apps, Multi Dimensional Acquisition Built Multi Dimensional Image from Sequential Images SEQ Notify user if some information will be lost during the save: MetaMorph Tiff MetaMorph Stack Molecular Devices Japan KK/ Imaging Team (7/12)

5. Measure Objects Enable Elliptical Fourier Parameters: Object Log Elliptical Fourier Parameter Measure All Regions: Integrated Morphometry Analysis Region Region Region Region Draw object borders: Color: Draw centroid mark: Color: Log Summery data on single line: Summery Log Redraw image during measurement: Draw failed classifier objects: Fill holes in objects: Integrated Morphometry Analysis Hole Area, Hole Area Hole Molecular Devices Japan KK/ Imaging Team (8/12)

Warning user when measurement will be erased: Exclude objects if centroid is not in the current region: Exclude objects that touch the edge of the image: Molecular Devices Japan KK/ Imaging Team (9/12)

6. Journals Display missing/extra data messages on journal playback: Load old Digital4 journal acquisition setting in to setting list: Digital4 Molecular Devices Japan KK/ Imaging Team (10/12)

7. Region Labels Draw labels next to regions: Region Label Location: Region Label Color: Region (Same as region) (Custom color) Label Background: Draw labels inside of region boundary: Region Region Draw labels with respect to the image zoom: Molecular Devices Japan KK/ Imaging Team (11/12)

8. Stack Buffering Disk swapping: RAM RAM Quick file loading: UIC 3-21 JPR 6 TEL 03-5282-5266 FAX 03-5282-5262 Molecular Devices Japan KK/ Imaging Team (12/12)