PCR StepOnePlus /StepOne PCR
PCR PCR StepOnePlus StepOne PCR PCR
Features At A Glance StepOne StepOnePlus / 48 96 FAM /SYBR Green dyes VIC /JOE dyes ROX dyes 1. NED /TAMRA dyes VeriFlex Block StepOnePlus StepOne PCR 48 StepOne 96 StepOnePlus 1 PCR 1 PCR PCR 2 99.7 9 logs 30 L 10 RNase P StepOne StepOne 96 StepOne StepOnePlus PCR StepOne StepOnePlus 3 48StepOne 4 96 StepOnePlus PCR LED FAM SYBR Green VIC JOE ROX SNP / StepOnePlus TAMRA 40 Fast PCR LCD USB E StepOnePlus VeriFlex PCR 6 StepOne StepOnePlus
StepOnePlus StepOne USB LAN Local Area Network E E StepOnePlus StepOne 5 LAN 4 1. 2. 3. 4. LAN 5. 2. 3. LAN 4. StepOne 5
StepOnePlus StepOne PCR 5 4 CT PCR SNP E & PowerPoint Excel jpeg 5. StepOne Multiple Plots 1 4 Multiple Plots SNP 6. StepOne PCR StepOne LAN
StepOnePlus StepOne FAM VIC TaqMan MGB VIC TAMRA SYBR Green I StepOnePlus TAMRA TaqMan MGB SYBR Green I 1 StepOnePlus StepOne - - - CT CT StepOnePlus StepOne SNP microrna StepOnePlus StepOne Fast 2 Fast 40 PCR Fast 10 Amplification Plot Allele Discrimination Plot (SNP Assay: SNP Assay 1) 4.1 3.6 3.1 ΔRn 1 Allele 2 2.6 2.1 1.6 1.1 0.1 0.6 0.1 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Cycle 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Allele 1 Homozygous 1/1 Heterozygous 1/2 Homozygous 2/2 Undeterminded 7. PCR StepOne 10 18S rrna TaqMan 9 log 8. SNP CYP2C19*2 TaqMan SNP Genotyping Assays StepOnePlus
VeriFlex 96 StepOnePlus PCR 6 VeriFlex VeriFlex PCR PCR VeriFlex PCR 6 VeriFlex VeriFlex (96 ) PCR VeriFlex StepOnePlus StepOne PCR StepOnePlus StepOne 5,000 10,000 DNA 99.7 StepOnePlus StepOne 79log 3.4 Melt Curve 10 Multiplex Assays 2.9 Derivative Reporter (-Rn ) 2.4 1.9 1.4.9 ΔRn 1 0.1.4 -.1 0.01 65.0 70.0 75.0 80.0 85.0 90.0 95.0 Tm: 79.2 Temperature ( C) 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 Cycle 9. PCR 18S GAPDH 3 Tm 10. StepOnePlus TaqMan 18S rrna VIC TGF- FAM cdna 48
2 < depth: 48.5 cm < depth: 48.5 cm < height: 51.8 cm > > < height: 51.8 cm > > < > width: 24.6 cm < > width: 24.6 cm StepOne 48 3 StepOnePlus StepOnePlus 96 4 VeriFlex
PCR / StepOnePlus StepOne PCR PCR StepOnePlus StepOne PCR 1 StepOnePlus StepOne StepOne StepOnePlus Ambion Applied Biosystems RNA Ambion Applied Biosystems RNA Ambion RNA TaqMan MicroRNA Assays microrna TaqMan TaqMan MicroRNA Assays MicroRNA Human Mouse Rat Arabidopsis Drosophila C. elegans mirna.appliedbiosystems.com TaqMan SNP Genotyping Assays TaqMan SNP Genotyping Assays 450 250 HapMap SNPs 70,000 csnps 160,000 2,500 Drug Metabolism Genotyping Assays Custom TaqMan SNP Genotyping Assays SNP TaqMan SNP Genotyping Assays www.allsnps.com Primer Express Applied Biosystems Primer Express v3.0 TaqMan PCR SYBR Green I Primer Express StepOnePlus StepOne
TaqMan Gene Expression Assays 700,000 PCR TaqMan Gene Expression Assays TaqMan Assays PCR PCR Custom TaqMan Gene Expression Assays TaqMan Gene Expression Assays www.allgenes.com TaqMan Gene Expression, SNP Genotyping, and MicroRNA Assays TaqMan Assays Gene Expression* SNP Genotyping MicroRNA TaqMan Yes Yes Yes InventoriedMade-to-Order Custom TaqMan Assays Yes Yes No Human > 2,024,000 > 4,000,000 > 300 Mouse > 179,000 > 10,000 > 240 Rat > 128,000 > 180 Drosophila melanogaster > 38,000 > 50 Arabidopsis thaliana > 95,000 > 40 C. elegans > 90,000 > 60 Canine > 6,000 N/A Rhesus macaque > 70 N/A * mirna Gene copy number Mitochondrial HapMap drug metabolism genotyping SNP TaqMan 11.
TaqMan Fast Universal PCR Master Mix TaqMan Universal PCR Master Mix Power SYBR Green PCR Master Mix 96 StepOnePlus /StepOne PCR Material List 11 Applied Biosystems StepOnePlus /StepOne PCR MicroAmp 48-Well Optical Adhesive Film 25 4375928 MicroAmp 48-Well Optical Adhesive Film 100 4375323 MicroAmp 96-Well Optical Adhesive Film 25 4360954 MicroAmp 96-Well Optical Adhesive Film 100 4311971 MicroAmp Fast Optical 48-Well Reaction Plate 20 4375816 MicroAmp Fast Optical 96-Well Reaction Plate with Barcode (0.1 ml) 20 4346906 MicroAmp Fast Optical 96-Well Reaction Plate with Barcode (0.1 ml) 200 4366932 8 MicroAmp Fast 8-Tube Strip, 0.1 ml 125 4358293 MicroAmp Optical 8-Cap Strip 300 4323032 MicroAmp Fast 48-Well Tray 10 4375282 MicroAmp 96-Well Tray for Veriflex Blocks 10 4379983 MicroAmp 48-Well Base Adaptor 5 4375284 Power SYBR Green PCR Master Mix 5mL 4367659 TaqMan Gene Expression Master Mix 5mL 4369016 TaqMan Genotyping Master Mix 10mL 4371355 TaqMan Fast Universal PCR Master Mix 250 4352042 TaqMan One-Step RT-PCR Master Mix Reagents Kit 200 4309169 High Capacity cdna Reverse Transcription Kit 200 4368814
Ordering Information StepOne PCR StepOnePlus PCR StepOnePlus PCR * StepOne-01 StepOnePlus-01 *StepOne PCR PCR http://www.appliedbiosystems.jp NOTICE TO PURCHASER: The StepOne [and StepOnePlus ] Real-Time PCR System is [Systems are ] covered by one or more of US Patents Nos. 5,038,852, 5,333,675, 5,656,493, 5,475,610, 5,602,756, 6,703,236, 7,238,517, 6,814,934, and corresponding claims in their non-us counterparts, owned by Applera Corporation. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5' nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. http://www.appliedbiosystems.jp The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Applied Biosystems, AB (Design), Primer Express and VIC are registered trademarks and Applera, FAM, JOE, MicroAmp, NED, StepOne, StepOnePlus, TAMRA and VeriFlex are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc. SYBR is a registered trademark of Molecular Probes, Inc. 2011, Life Technologies Japan Ltd. All rights reserved. Printed in Japan, 12/2011, SDS024-B1112OB 108-0023 4-2-8 564-0052 10-28 TEL.03 6832 9300 FAX. 03 6832 9580 TEL.06 6389 1201 FAX. 06 6389 1206 http://www.lifetechnologies.co.jp