Live Cell Imaging EVOS FL Auto Imaging System with Onstage Incubator Invitrogen CellLight BacMam 2.0 Molecular Probes
EVOS FL Auto Imaging System with Onstage Incubator EVOS FL Auto Imaging System PCIncubator Incubator LED Water reservoir USB EVOS FL Auto Imaging SystemOnstage Incubator Sensor data cable jack USB control cable jack Power switch Power input jack Port 1: Air Port 2: N 2 Port 3: CO 2
EVOS FL Auto + 92cm 92cm Onstage Incubator 37 16 cm EVOS FL Auto 3 1. Incubate 2. Enable Environmentalchamber * 3. OK * X Y Z Time Lapse tab Enable Enviroment Chamber Adjusting/OK LED LED50,000 35mm 60mm T-25 40 0.1 CO2 0% 20% O2 0% 3780% RH
EVOS FL Auto Imaging System with Onstage Incubato EVOS FL Auto Imaging System LCD LED 50,000 5 60 mm X/Y 22 CCD CMOS 1/2 2,048 x 1,536 3.1 16 TIFF PNG 12 24 TIFF PNG JPEG BMP 1,280 x 960 WMV 16 TIFF PNG 12 24 TIFF PNG JPEG BMP 1,360 x 1,024 USB x 1 DVI USB AC 322 mm 472 mm 343 mm 920mm 920mm 20.0 kg Ordering Information Light Cube XY EVOS FL Auto 4 Ph, 10 Fl, 20 Fl, 40 Fl GFP, RFP, DAPI & 6,800,000 EVOS Onstage Incubator 35 mm 60 mm T-25 40 CO 2 0 20 % O 2 0 % 80 % 37 25 19 3.7 cm 37 16 20 cm 1.5 kg 10 kg Ordering Information EVOS FL Auto System EVOS Onstage Incubator 1 AMC1000 1,690,000 2015 9
CellLight BacMam 2.0 BacMam Invitrogen CellLight BacMam 2.0 - GFP/RFP/CFP Structure Targeting sequence CFP 440/480* GFP 488/510* RFP 555/584* 1 ml 1 ml 0.1 ml 1 ml 0.1 ml Actin Human actin C10582 C10506 C10583 C10502 Cytoplasm B10383 Endoplasmic Reticulum ER signal sequence of calreticulin and KDEL ER retention signal C10590 C10591 Early Endosomes Rab5a C10586 C10587 Late Endosomes C10588 C10589 Golgi Human Golgi-resident enzyme N-acetylgalactosaminyltransferase 2 C10592 C10593 Histones Histone 2B Histone 2B C10594 C10595 Lysosomes Lamp1 lysosomal associated membrane protein 1 C10596 C10507 C10597 C10504 Mitochondria Leader sequence of E1 alpha pyruvate dehydrogenase C10600 C10601 Nucleus SV40 nuclear localization sequence C10616 C10602 C10603 Peroxisomes Peroxisomal C-terminal targeting sequence C10604 Plasma Membrane Myristolyation/palmitoylation sequence from Lck tyrosine kinase C10606 C10607 C10608 Synaptophysin Synaptophysin C10609 C10610 Talin Human c-terminus of talin C10611 C10612 Tubulin Human tubulin C10613 C10509 C10614 C10503 BacMam 2.0 Transduction Control B10383 Null virus control C10615 1mL Ordering information CellLight control 1ml 0.1ml BacMam GFP transduction control 1ml B10383 BacMam Enhancer Kit 120ul 2 B10107 1mlBacMam 1 10 8 1101 10 7 100mm dish 2.5 35mm dish 12.5 96well 125 well BacMam テクノロジーで実績のある細胞種 ヒト : Astroglioma U373MG HEK 293 HeLa HepG2 HUVEC Keratinocytes MG63 MRC5 CRL-1973 W12 WI38 COS-7 CV-1 Vero Pancreatic β-cells Prenatal cardiomyocytes Primary dendritic cells Primary hepatic stellate cells Primary hepatocytes Primary neural cells Primary umbilical vein endothelial cell Bone marrow stem cells Umbilical cord blood stem cells HAEC HEKn HDFa SHSY-5Y RAW T84 Calu-3 全 75 種 ハムスター : ラット : マウス : CHO, CHO K1, CHO M1WT3, etc. BHK RGM I PC12 Primary rat chondrocytes Primary hepatic stellate cells Rat2 全 18 種 L929 Mouse pancreatic β-cells Mouse primary kidney cells N2a NIH 3T3 Primary rat hepatocytes Rat Brain Pericytes EL4 全 16 種 造血細胞 マクロファージには導入できません この他にラビット ラットカンガルー ブタ ウシ ヒツジ シカ キツネ イヌなどの細胞に導入実績があります 詳細についてはホームページをご覧下さい
Molecular Probes Molecular Probes Ex/Em MitoTracker Orange CM-H2TMRos 554/576 M7511 MitoTracker Orange CMTMRos 554/576 M7510 MitoTracker Red CM-H2XRos 579/599 M7513 MitoTracker Red CMXRos 579/599 M7512 MitoTracker Deep Red 644/665 M22426 MitoTracker Green FM 490/516 M7514 MitoTracker Red 581/644 M22425 JC-1 Image-iT LIVE Mitochondrial and Nuclear Labeling Kit 514/529 monomer form; 585/590 J-aggregate form 579/599 MitoTrackr Red CMXRos ; 350/461 Hoechst 33342 T3168 I34154 MitoTracker Red CMXRos Hoechst 33342 LysoTracker Blue DND-22 373/422 L7525 ph LysoTracker Yellow HCK-123 465/535 L12491 LysoTracker Green DND-26 504/511 L7526 LysoTracker Red DND-99 577/590 L7528 ph 1-5 ph LysoSensor Blue DND-167 373/425 L7533 ph LysoSensor Green DND-153 442/505 L7534 LysoSensor Yellow/Blue dextran 335, 381 /452, 521 L22460 LysoSensor Yellow/Blue DND-160 329, 384 /440, 540 L7545 Image-iT LIVE Lysosomal and Nuclear Labeling Kit NBD C6-ceramide, NBD C6-ceramide complexed to BSA BODIPY FL C5-ceramide BODIPY FL C5-ceramide complexed to BSA BODIPY TR ceramide BODIPY TR ceramide complexed to BSA 577/590 LysoTracker Red DND-99 350/461 Hoechst 33342 dye 466/536 505/511 589/617 I34202 N1154 N22651 D3521 B22650 D7540 B34400 ph 365+/-8nm450+/-33nm 510+/-20nm 365+/-8nm450+/-33nm 510+/-20nm Contains LysoTracker Red DND-99 Hoechst 33342 NBD C6-ceramide * nm 2 2
MitoFluor Far Red 680 LysoTracker Green DND-26 and Hoechst 33342 Madin-Darby(MDCK) BODIPY FL C5-ceramide LysoTracker Red DND-99 Hoechst 33258 Ex/Em TubulinTracker Green Reagent 494/522 T34075 Hoechst 33342 350/461 H1399 SYTO RNASelect Green Stain 490/530 S32703 SYTO 59 Stain 622/645 S11341 SYTO 82 Stain 541/560 S11363 SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit SYTO Green Fluorescent Nucleic Acid Stain Sampler Kit SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit - S11350 SYTO 40-45 Oregon Green 488 paclitaxel Oregon Green 488 paclitaxel Hoechst33342 RNA - S7572 SYTO 11-14, 16, 21, 24, 25 - S11360 SYTO 80-85 - S11340 SYTO 17, 59-64 ER-Tracker Blue-White DPX Dye 374/430-640 E12353 ER-Tracker Green 504/511 E34251 ER-Tracker Blue-White DPX ER-Tracker Red 587/615 E34250 Wheat germ agglutinin WGA conjugates 346/442 W11263 Alexa Fluor 488 495/519 W11261 Alexa Fluor 555 555/565 W32464 Alexa Fluor 594 590/617 W11262 Alexa Fluor 647 650/665 W32466 Alexa Fluor 680 679/702 W32465 WGA(wheat Germ Agglutinin) WGA FM 1-43 510/626 T3163 FITC FM 1-43 FX 510/626 F35355 FM 1-43 FM 4-64 558/734 T3166 FM 4-64 FX 565/744 F34653 FM 4-64 Image-iT LIVE Plasma Membrane and Nuclear Labeling Kit 590/617 WGA Alexa Fluor 594 ; 350/461 Hoechst 33342 I34406 CellTrace BODIPY TR Methyl Ester 598/625 C34556 Image-iT LIVE Intracellular Membrane and Nuclear Labeling Kit 598/625 CellTrace BODIPY TR Methyl Ester ; 350/461 Hoechst 33342 I34407 WGA Alexa Fluor 594Hoechst 33342 GFP CellTrace BODIPY TR Methyl Ester Hoechst 33342 * nm 650nm 650nm
Molucular Probes St. Paul Kitchen 1975Molecular Probes 1985 Molecular ProbesAlexa Fluor SYBR Qdot BacMam Click-iT 1978 年 2 月 Molecular Probes ハンドブック A Guide to Fluorescent Probes and Labeling Technologies ( 蛍光プローブと標識テクノロジーの手引き ) 初版をリリース 1979 年 7 月最初の BioProbes ニュースレターをリリース 1996 年 Oregon Green 色素を発売 2003 年 Molecular Probes ブランドが Invitrogen Corporation の一部に 2011 年 Molecular Probes の標識 検出テクノロジーは 当社の主要ブランドの 1 つであり続ける 1975 年 Molecular Probes が ミネソタ州セントポールの Richard & Rosaria Haugland のキッチンで誕生 1989 年現在のオレゴン州ユージーンへ移転 Cascade Blue 色素を発売 1985 年テキサス州プラノへ移転 Texas Red 色素を開発 オレゴン州ジャンクションシティへ移転 1999 年 Alexa Fluor 色素を発売 2008 年 Invitrogen Corporation と Applied Biosystems Inc. が合併し Life Technologies, Inc. が誕生 2010 年 9 月 Molecular Probes ハンドブック A Guide to Fluorescent Probes and Labeling Technologies ( 蛍光プローブと標識テクノロジーの手引き ) 第 11 版をリリース Molecular Probes QRMolecular Probes Web Molecular Probes Specra Viewer SpectraViewer www.thermofisher.com/spectraviewer www.thermofisher.com/tc For Research Use only.not for use in diagnostic procedures. 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Printed in Japan. MP070-B1509OB 108-0023 4-2-8 0120-477-392 jptech@thermofisher.com TEL 03-6832-6980 FAX 03-6832-9584 TEL 03-6832-9300 FAX 03-6832-9580 facebook.com/thermofisherjapan @ThermoFisherJP www.thermofisher.com