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- のぶあき わくや
- 5 years ago
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1 2010
2 Caspase DNA DNA V13240 Vybrant Apoptosis Assay Kit #1 *Alexa Fluor 488 annexin V/SYTOX Green* *50 assays* V13241 Vybrant Apoptosis Assay Kit #2 *Alexa Fluor 488 annexin V/ propidium iodide* *50 assays* V13242 V13243 V13244 V23201 Vybrant Apoptosis Assay Kit #3 *FITC annexin V/propidium iodide* *50 assays* Vybrant Apoptosis Assay Kit #4 *YO-PRO-1/propidium iodide* *200 assays* Vybrant Apoptosis Assay Kit #5 *Hoechst 33342/propidium iodide* *200 assays* Vybrant Apoptosis Assay Kit #7 *Hoechst 33342/YO-PRO-1/ propidium iodide* *200 assays* V35112 Vybrant Apoptosis Assay Kit #8 *R-phycoerythrin annexin V/ SYTOX Green* *50 assays* V35113 Vybrant Apoptosis Assay Kit #9 *allophycocyanin annexin V/ SYTOX Green* *50 assays* V35114 Vybrant Apoptosis Assay Kit #10 *allophycocyanin annexin V/ C12-resazurin/SYTOX Green* *50 assays* V35116 Vybrant Apoptosis Assay Kit #11 *Alexa Fluor 488 annexin V/ MitoTracker Red CMXRos* *50 assays* V35123 Vybrant Apoptosis Assay Kit #13 *PO-PRO-1/7- aminoactinomycin D* *for flow cytometry* *200 assays* Single Channel Annexin V/ Dead Cell Apoptosis Kit *Alexa Fluor 488 annexin V/SYTOX Green* *50 assays* *for flow cytometry* Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit *Alexa Fluor 488 annexin V/propidium iodide* *50 assays* *for flow cytometry* FITC Annexin V/ Dead Cell Apoptosis Kit *FITC annexin V/propidium iodide* *50 assays* *for flow cytometry* Membrane Permeability/ Dead Cell Apoptosis Kit *YO-PRO -1/ propidium iodide* *200 assays* *for flow cytometry* Chromatin Condensation/Dead Cell Apoptosis Kit *Hoechst 33342/propidium iodide* *200 assays* *for flow cytometry* Chromatin Condesation/Membrane Permeability/Dead Cell Apoptosis Kit. *Hoechst 33342/YO-PRO -1/propidium iodide* *200 assays* *for flow cytometry* PE Annexin V/ Dead Cell Apoptosis Kit *with SYTOX Green* *50 assays* *for flow cytometry* APC Annexin V/Dead Cell Apoptosis Kit *with APC annexin V and SYTOX Green* *50 assays* *for flow cytometry* Metabolic Activity/Annexin V/Dead Cell Apoptosis Kit *with C12 resazurin, APC annexin V, and SYTOX Green* *50 assays* *for flow cytometry* Mitochondrial Membrane Potential/Annexin V Apoptosis Kit *Alexa Fluor 488 annexin V/MitoTracker Red CMXRos* *50 assays* *for flow cytometry* Violet Membrane Permeability/Dead Cell Apoptosis Kit *with PO-PRO -1 and 7- aminoactinomycin D* *200 assays* *for flow cytometry*
3 92 / Invitrogen Synechocystis sp. PCC 6803 BODIPY FL Synechococcus sp. PCC 7942 DAPI Anabaena cylindrica CellTracker Green BODIPY BODIPY FL University College, London Mary Sarcina DNA DNA M camptothecin Jurkat annexin V propidium iodide SYTOX Green Dye Chapter
4 / PS V PS Vybrant V V Propidium iodide fluores V D A Propidium iodide fluores V D A Alexa Fluor 488 fluorescenc e Alexa Fluor 488 fluorescenc e Jurkat T 1μM external Alexa Fluor 488 annexin V (A13201) propidium iodide Vybrant #2 A V D Platform Laser (nm) Ex/Em Alexa Fluor 350 FC, I UV, / μl A23202 Pacific Blue FC, I / μl A35122 Alexa Fluor 488 FC, I / μl A13201 FITC FC, I / μl A13199 Alexa Fluor 555 FC, I / μl A35108 Alexa Fluor 568 FC, I / μl A13202 Alexa Fluor 594 FC, I / μl A13203 Alexa Fluor 647 FC, I / μl A23204 R-PE FC, I 488, , 546, 565/ μl A35111 APC FC, I / μl A35110 FC = flow cytometry; I = imaging; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm. 98
5 SYTOX A ADvance d SYTOX A ADvance d / 2010 Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit F2N12S F2N12S PS 2 Shynkar, V.V., et al. J. Am. Chem. Soc : V A B 262, , , ,608 A+D A+D 131, ,072 65,536 65,536 L L , , , , , , , , D D A L 10 3 L A Chapter ,200 2,500 3,750 5, ,200 2,500 3,750 5,000 F2N12S ratio 585 nm/530 nm F2N12S ratio 585 nm/530 nm Jurkat 10 M 4 B D A C F2N12S 405nm 585nm 530nm SYTOX AADvanced 488nm 695nm A B F2N12S 2 C D SYTOX AADvanced F2N12S 2 585/530nm A L D Platform Laser (nm) Ex/Em Violet Ratiometric Membrane Asymmetry Probe/ Dead Cell Apoptosis Kit# FC 405 [F2N12S] 405/530 [ ], 405/585 [ ] 100 μl A35137 # SYTOX AADvanced dead cell stain Ex/Em = 546/647 nm) 98+
6 / V V (PO-PRO.-1, YO-PROR-1, TO-PROR-3) 3 Platform Cell status Laser (nm) Ex/Em PO-PRO -1 Iodide FC, I Live /455 1mL P3581 YO-PRO -1 Iodide FC, I Live /509 1mL Y3603 TO-PRO -3 Iodide FC, I Live /661 1mL T3605 FC = flow cytometry; I = imaging; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm. Dapoxyl 50 nm LysoTracker Blue-White DPX 100 nm MitoTracker CMXRos Huygens Scientific Volume Imaging Basal 5 min 10 min 20 min MitoTracker MitoTracker MitoTracker DNA in situ JC-1 NIH 3T3 H2O2 J- Ildo Nicoletti Perugia University Medical School MitoTracker Red CMXRos 579 nm 599 nm MitoTracker Orange CM Ros MitoTracker Red CM-H2XRos MitoTracker Orange CM-H2 Ros MitoTracker MitoTracker Orange MitoTracker Red GFP GFP MitoTracker JC nm 590 nm nm TMRM TMRE 99
7 JC-1 red fluoresce nce JC-1 red fluoresce nce / Image-iT LIVE MitoProbe calcein AM calcein CoCl2 calcein AM calcein AM calcein CoCl2 calcein calcein Image-iT LIVE MitoTracker Red CMXRos A B JC-1 green fluorescence C C SelectFX Alexa Fluor 488 C C C Alexa Fluor Science 292, ; 2. Science 289, ; 3. Trends Cell Biol 10, ; 4. Eur J Biochem 252, ; 5. J Histochem Cytochem 44, ; 6. Cytometry 35, ; 7. Proc Natl Acad Sci U S A 77, ; 8. Int Rev Cytol 122, JC-1 green fluorescence MitoProbe JC-1 M34152 Jurkat Jurkat M JC-1 PBS 488nm 530nm 585nm A B M camptothecin p Chapter 4 Platform Cell status Fixable Ex/Em MitoTracker Green FM FC, I Live No 490/ x 50 μg M7514 MitoTracker Orange CMTMRos FC, I Live Yes 554/ x 50 μg M7510 MitoTracker Orange CM-H 2 TMRos FC, I Live Yes 554/ x 50 μg M7511 MitoTracker Red CMXRos FC, I Live Yes 579/ x 50 μg M7512 MitoTracker Red CM-H 2 XRos FC, I Live Yes 579/ x 50 μg M7513 JC-1 FC, I Live No 514/529 5 mg T3168 MitoProbe DiOC 2 (3) Assay Kit FC Live No 482/497 1 kit M34150 MitoProbe DiIC 1 (5) Assay Kit FC Live No 638/658 1 kit M34151 MitoProbe JC-1 Assay Kit FC Live No 514/529 1 kit M34152 Tetramethylrhodamine, methyl ester, perchlorate (TMRM) FC, I Live No 549/ mg T668 Tetramethylrhodamine, ethyl ester, perchlorate (TMRE) FC, I Live No 549/ mg T669 Dihydrorhodamine 123 FC, I Live No 507/529 1 ml D23806 MitoProbe Transition Pore Assay Kit FC Live No 494/517 1 kit M34153 Image-iT LIVE Mitochondrial Transition Pore Assay Kit I Live No 494/517 1 kit I35103 HCS Mitochondrial Health Kit HCS Live Yes 550/580 1 kit H10295 FC = flow cytometry; HCS = high-content screening; I = imaging; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm
8 / Caspase Caspase Vybrant FAM Caspase caspase caspase FLICA FLICA caspase caspase FMK FLICA FAM FLICA caspase FMK FLICA FLICA caspase Vybrant FAM Caspase caspase caspase Vybrant FAM Caspase caspase FLICA caspase FLICA caspase L A D Platform Caspase target Fluorescent molecule Vybrant FAM Poly Caspases Assay Kit FC Generic Fluorescein 1 kit V35117 Vybrant FAM Caspase-3 and -7 Assay Kit FC 3, 7 Fluorescein 1 kit V35118 Vybrant FAM Caspase-8 Assay Kit FC 8 Fluorescein 1 kit V35119 Image-iT LIVE Green Poly Caspases Detection Kit I Generic Fluorescein 1 kit I35104 Image-iT LIVE Green Caspase-8 Detection Kit I 8 Fluorescein 1 kit I35105 Image-iT LIVE Green Caspase-3 and -7 Detection Kit I 3, 7 Fluorescein 1 kit I35106 Image-iT LIVE Red Poly Caspases Detection Kit I Generic Rhodamine 1 kit I35101 Image-iT LIVE Red Caspase-3 and -7 Detection Kit I 3, 7 Rhodamine 1 kit I35102 FC = flow cytometry; I = imaging. 101
9 97 98 / Hoechst UV V13244 Platform Cell status Laser (nm) Ex/Em Hoechst FC, I Live UV 350/ ml H3570 Vybrant DyeCycle Violet Stain FC Live / μl V35003 Propidium Iodide FC, I Fixed UV, 405, 488, / ml P3566 Chromatin Condensation Apoptosis Kit, with Hoechst and PI Chromatin Condensation/Membrane Permeability/Dead Cell Apoptosis Kit, Hoechst 33342, YO-PRO -1, and PI Violet Chromatin Condensation/Dead Cell Apoptosis Kit, with Vybrant DyeCycle Violet and SYTOX AADvanced FC = flow cytometry; I = imaging; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm. FC Live UV/ /461 (apoptotic) 535/617(dead) FC Live UV/ /461 (live) 491/509 (apoptotic) 535/617 (dead) FC Live 405/ /437 (apoptotic) 546/647 (dead) 1 kit V kit V kit A35135 Chapter
10 / DNA DNA TUNEL 1,2 DNA DNA laddering DNA TUNEL DNA TUNEL TdT DNA BrdUTP BrdU anti-brdu APO-BrdU TUNEL BrdU Alexa Fluor 488 anti-brdu Click-iT TUNEL Click-iT 2 APO-BruU TUNEL TUNEL HL-60 DNA ChromaTide BODIPY FL-14-dUTP TUNEL Zbigniew Darzynkiewicz Cancer Research Institute, New York Medical College Platforms # of assays APO-BrdU TUNEL Assay Kit, with Alexa Fluor 488 anti-brdu FC 60 1 kit A23210 APO-BrdU TUNEL Assay Kit, with FITC anti-brdu FC 60 1 kit KHO1001 Click-iT TUNEL Alexa Fluor 488 Imaging Assay I/HCS kit C10245 Click-iT TUNEL Alexa Fluor 594 Imaging Assay I/HCS kit C10246 Click-iT TUNEL Alexa Fluor 647 Imaging Assay I/HCS kit C10247 FC = flow cytometry, HCS = high-content screening, I = imaging. 103
11 / DNA Invitrogen Vybrant Vybrant 2 3 C12- MitoTracker Red CMXRos Hoechst YO-PRO -1 SYTOX Green Vybrant Vybrant 10 4 D 10 4 PI fluorescence V Live Apoptotic Dead A YO-PRO -1 fluorescenc e Jurkat T 10 M 4 V SY TOX Green fluorescenc e Jurkat T 10 M 2mM 4 V35114 Viable cell stain Apoptotic cell stain Single Channel Annexin V/Dead Cell Apoptosis Kit, with Alexa Fluor 488 annexin V and SYTOX Green Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit, with PI None None Annexin V-Alexa Fluor 488 Annexin V-Alexa Fluor 488 Dead cell stain Excitation (nm) SYTOX Green 488 V13240 PI 488 V13241 Chapter 4 FITC Annexin V/Dead Cell Apoptosis Kit, with PI None Annexin V-FITC PI 488 V13242 Membrane Permeability/Dead Cell Apoptosis Kit, with YO-PRO -1 and PI None YO-PRO -1 dye PI 488 V13243 Chromatin Condensation Apoptosis Kit, with Hoechst and PI Hoechst (dim) Hoechst (bright) PI UV/488 V13244 Chromatin Condensation/Membrane Permeability/Dead Cell Apoptosis Kit, with Hoechst 33342, YO-PRO -1, and PI Hoechst (dim) YO-PRO -1 dye Hoechst 3342 (bright) PI UV/488 V23201 PE Annexin V/Dead Cell Apoptosis Kit, with SYTOX Green None Annexin V-PE SYTOX Green dye 488 V35112 APC Annexin V/Dead Cell Apoptosis Kit, with SYTOX Green Metabolic Activity/Annexin V/Dead Cell Apoptosis Kit, with C 12 resazurin, APC annexin V, and SYTOX Green None Annexin V-APC SYTOX Green dye C 12 -resazurin (red) Annexin V-APC SYTOX Green dye 488/633 V /633 V35114 Mitchondrial Membrane Potential/Annexin V Apoptosis Kit, with MitoTracker Red and Alexa Fluor 488 annexin V MitoTracker Red Annexin V-Alexa Fluor 488 None 488 V35116 Violet Annexin V/Dead Cell Apoptosis Kit, with Pacific Blue annexin V/SYTOX AADvanced None Annexin V-Pacific Blue SYTOX AADvanced 405/488 A35136 Violet Membrane Permeability/Dead Cell 1 Apoptosis Kit, with PO-PRO -1 and 7-aminoactinomycin D None PO-PRO -1 SYTOX AADvanced 405/488 V35123 Violet Chromatin Condensation/Dead Cell Apoptosis Kit, with Vybrant DyeCycle Violet and SYTOX AADvanced Vybrant DyeCycle Violet (dim) Vybrant DyeCycle Violet (bright) SYTOX AADvanced 405/488 A
12 / DNA 3 G0/G1 1 S DNA DNA G2/M 1 DNA DNA DNA- Vybrant DyeCycle Vybrant DyeCycle DNA Vybrant DyeCycle DNA DNA DNA DNA Vybrant DyeCycle Vybrant DyeCycle Vybrant DyeCycle 488nm 405nm Vybrant DyeCycle Violet 405nm UV Vybrant DyeCycle Green - 488nm Vybrant DyeCycle Orange 488nm 532nm Vybrant DyeCycle 2009 FxCycle FxCycle 488nm 108
13 H3-Alexa Fluor / p Vybrant DyeCycle Violet Jurkat DNA G 0 /G 1 G 2 /M S 440/40 405nm TF-1 0.1% Triton X-100/ PBS/1% BSA Zenon Alexa Fluor 488 Rabbit IgG labeling reagent H3[pS10] G FxCycle Violet Stain ph3 Platform Cell status Laser (nm) Ex/Em Vybrant DyeCycle Violet Stain FC Live UV, / assays V35003 Vybrant DyeCycle Green Stain FC Live / assays V35004 Vybrant DyeCycle Orange Stain FC Live 488, / assays V35005 Vybrant DyeCycle Ruby Stain FC Live 488, 633/ / assays V assays V10309 FC = flow cytometry; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm. Platform Cell status Laser (nm) Ex/Em FxCycle Violet Stain FC Fixed UV, / assays F10347 FxCycle Far Red Stain FC Fixed 633/ / assays F10348 FC = flow cytometry; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm. Chapter
14 / DNA Click-iT EdU Click-iT EdU BrdUEdU 5- -2'- DNA DNA EdU DNA EdU EdU Alexa Fluor 488 S Click-iT EdU DNA BrdU DNA EdU Click-iT DNA EdU 114
15 C lick-it lexa Fluo r fluorescence nti-brdu Alexa Fluor fluorescence / Click-iT Click-iT EdU Assay Kit DNA S DNA BrdU, EdU BrdU EdU (Alexa Fluor 488 azide) DNA EdU BrdU -BrdU DNA A AAD fluorescence A AAD fluorescence Click-iT EdU for Flow Cytometry Jurkat EdU Click-iT EdU Alexa Fluor 488 Assay Kit for Flow Cytometry FACScan BrdU Chapter 4 EdU EdU ( ) EdU ( ) EdU 30 ( ) Click-iT EdU for Flow Cytometry EdU GFP -GFP PE EdU 131
16 / Click-iT EdU DNase HCl 3 Click-iT EdU for Imageing Kit Click-iT EdU BrdU Click-iT EdU Click-iT EdU Alexa Fluor 594 Hoechst3342. EdU BrdU 160 μg/g ( ~20 mg) BrdU HCl DNase Anti-BrdU Alexa Fluor 594 Hoechst Click-iT EdU for HCS Assay HeLa 10 M EdU 2 Click-iT EdU Alexa Fluor 488 azide ( ) Alexa Fluor DAPI 5 Click-iT EdU for Imaging Kit, in vivo 2 Alexa Fluor 488 Imaging Kit Edu 1 ~250 l DAPI Sima Zacharek, Children's Hospital Boston 6 Muntjac ( Click-iT Edu Alexa Fluor 647 HCS assay kit 10 M EdU 45 DNA Far Click-iT EdU Alexa Fluor 647 High-Throughput (HCS) Assay Alexa Fluor 350 goat Anti-mouse IgG green fluoresecent lectin HPA from Helix pomatia, Alexa Fluor 488 Peroxisomes peroxisome antibody Alexa Fluor 555 donkey rabbit IgG 7 Click-iT EdU Alexa Fluor 488 Imaging Kit DAPI Olympus FluoView FV nm 488 nm UPLSAPO 60x (NA:1.35) 1.28 m/slice DIC DNA EdU Alexa Fluor 488 dye DAPI Ferhan Ayaydin Cellular Imaging Laboratory, Biological Research Center, Szeged, Hungary 132
17 / Imaging Imaging Imaging Imaging EdU 5mg 5mg 5mg 5mg Alexa Fluor azide Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 594 Alexa Fluor 647 ComponentG Hoechst 3342 Hoechst 3342 Hoechst 3342 Hoechst 3342 Assay 50 coverslips 50 coverslips 50 coverslips 50 coverslips / 495/ / / /670 nm Hechst:350/461 Hechst:350/461 Hechst:350/461 Hechst:350/461 HCS HCS HCS HCS HCS HCS EdU 255 g 1.28 mg 255 g 1.28 mg 255 g 1.28 mg Alexa Fluor azide Fluor azide 488 Alexa Fluor 488 Alexa Fluor 594 Alexa Fluor 594 Alexa Fluor 647 Alexa Fluor 647 ComponentG Hoechst 3342 Hoechst 3342 Hoechst 3342 Hoechst 3342 Hoechst 3342 Hoechst 3342 Assay 2x96 test 10x96 test 2x96 test 10x96 test 2x96 test 10x96 test /495/ / / / / /670 nm Hechst:350/461 Hechst:350/461 Hechst:350/461 Hechst:350/461 Hechst:350/461 Hechst:350/461 Flow Cytometry Flow Cytometry Flow Cytometry EdU 10mg 10mg 10mg Alexa Fluor azide Component J Component K Alexa Fluor 488 Click-iT Edu Cell Cycle 633-red, Click-iT Edu Cell Cycle 488-red Alexa Fluor 647 Click-iT Edu Cell Cycle 405-blue Propidium iodide Pacific Blue Click-iT Edu Cell Cycle 633-red Click-iT Edu Cell Cycle 488-red Imaging Kit l 50 Platform Click-iT EdU Pacific Blue Flow Cytometry Assay Kit FC 50 assays A10034 Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit FC 50 assays C35002 Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit FC 50 assays A10202 Click-iT EdU Alexa Fluor 488 HCS Assay HCS 2 plates C plates C10351 Click-iT EdU Alexa Fluor 555 HCS Assay HCS 2 plates C plates C10353 Click-iT EdU Alexa Fluor 594 HCS Assay HCS 2 plates C plates C10355 Click-iT EdU Alexa Fluor 647 HCS Assay HCS 2 plates C plates C10357 Click-iT EdU Alexa Fluor 488 Imaging Kit I 50 coverslips C10337 Click-iT EdU Alexa Fluor 594 Imaging Kit I 50 coverslips C10339 Click-iT EdU Alexa Fluor 647 Imaging Kit I 50 coverslips C10340 Click-iT EdU Microplate Assay MP 400 assays C10214 EdU (5-ethynyl-2 -deoxyuridine) NA 50 mg A mg E10187 Chapter 4 FC = flow cytometry; HCS = high-content screening; I = imaging; MP = microplate; NA = not applicable
18 / (SE) SE SE (5(6)-CFDA SE CFSE) CFDA SE PKH26 CFDA SE 8 10 CellTrace Oregon Green 488 fluorescence 0 CellTrace 488 C ( ) 5 in vivo CFDA SE Invitrogen Vybrant CFDA SE 10 1 CFSE DMSO CellTrace CFSE CFSE 2010 CellTrace Violet Cell Proliferation Kit CFSE GFP CFSE 10 cells of Number Parent Generation 2 Generation 3 Generation 4 Generation 5 Generation 6 Generation 7 Generation 8 Generation 9 Generation Platform Laser (nm) Ex/Em CellTrace CFSE Cell Proliferation Kit FC, I / x 50 μg C34554 CellTrace Violet Cell Proliferation Kit FC, I /450 9 vials C
19 Number of cells counted Number of cells counted Number of cells counted Number of cells counted / Redox LIVE/DEAD LIVE/DEAD LIVE/DEAD Fixable Blue stain fluorescence LIVE/DEAD Fixable Violet stain fluorescence LIVE/DEAD Fixable Green stain fluorescence LIVE/DEAD Fixable Red stain fluorescence Platform Laser (nm) Ex/Em ArC Bead Kit for use with amine-reactive dyes FC NA NA 100 assays A10346 LIVE/DEAD Fixable Blue Dead Cell Stain Kit FC UV 350/ assays L23105 LIVE/DEAD Fixable Violet Dead Cell Stain Kit FC / assays L34955 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit FC / assays L34957 LIVE/DEAD Fixable Yellow Dead Cell Stain Kit FC / assays L34959 LIVE/DEAD Fixable Green Dead Cell Stain Kit FC / assays L23101 LIVE/DEAD Fixable Red Dead Cell Stain Kit FC / assays L23102 LIVE/DEAD Fixable Far Red Dead Cell Stain Kit FC 633/ / assays L10120 LIVE/DEAD Fixable Near IR Dead Cell Stain Kit FC 633/ / assays L10119 LIVE/DEAD Fixable Dead Cell Sampler Kit FC Various Various 300 assays L34960 FC = flow cytometry; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm. NA = not applicable. Chapter 4 SYTOX 4 SYTOX 1 DCD DCD
20 SYTOX Red fluorescenc / SYTOX Nucleic Acid Stains 10 5 D Invitrogen L A R-PE fluorescence Jurkat SYTOX Red Dead Cell Stain R- (R-PE) R-PE V SYTOX Red Dead Cell Stain 30 (L) apoptotic(a) (D) Platform Laser (nm) Ex/Em SYTOX Blue Nucleic Acid Stain FC, I / μl S11348 SYTOX Green Nucleic Acid Stain FC, I / μl S7020 SYTOX AADvanced Nucleic Acid Stain FC 488, / assays S assays S10274 SYTOX Orange Nucleic Acid Stain FC, I / μl S11368 SYTOX Red Nucleic Acid Stain FC, I /658 1 ml S34859 FC = flow cytometry; I = imaging; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm. CellTrace Calceins CellTrace calcein AM calcein AM ph Calcein BCECF CellTrace calcein AM calcein AM Calcein AM SYTOX Calcein Jurkat Jurkat CellTrace Calcein AM 405nm 450/
21 / Platform Laser (nm) Ex/Em CellTrace Calcein Blue, AM FC, I UV, /450 1,000 assays C34853 CellTrace Calcein Violet, AM FC, I /452 1,000 assays C34858 CellTrace Calcein Green, AM FC, I /517 1,000 assays C34852 CellTrace Calcein Red-Orange, AM FC, I /595 1,000 assays C34851 LIVE/DEAD Violet Viability/Vitality Kit FC, I /452(L); 367/526(D) 200 assays L34958 FC = flow cytometry; I = imaging; D = dead; L = live; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm. AM (AM) AM AM / Invitrogen LIVE/DEAD /2 AM nm AM nm 635 nm 1 LIVE/DEAD / AM 10 4 Chapter 4 Red fluorescence Dead cells Live cells Green fluorescence LIVE/DEAD / ( ) ( ) LIVE/DEAD / B 1 1 AM 1 488nm (530nm) (585nm) Platforms Live cell stain(ex/em*) Dead cell stain(ex/em*) LIVE/DEAD Viability/Cytotoxicity Kit I, MP, FC Calcein, AM (494/517) EthD-1 (528/617) 200 1,000 assays L3224 * Excitation (Ex) and emission (Em) maxima, in nm. I = Imaging. MP = Microplate. FC = Flow cytometry
22 / LIVE/DEAD reduced LIVE/DEAD reduced #1 SYTO 10 DEAD ( 2) SYTO 10 DEAD ( ) trinitrophenyl-sensitize nonsensitized dinitrophenyl-klh rabbit complement LIVE/ DEAD Reduced Biohazard Viability/Cytotoxicity Kit #1. membrane-permeant SYTO 10 membrane-impermeant DEAD Red dye membrane-compromised cells Platforms Live cell stain(ex/em*) Dead cell stain(ex/em*) LIVE/DEAD Reduced Biohazard Cell Viability Kit #1 I, FC SYTO 10 (484/505) EthD-2 (535/624) 100 assays L7013 * Excitation (Ex) and emission (Em) maxima, in nm. I = Imaging.. FC = Flow cytometry. LIVE/DEAD LIVE/DEAD calcein NK - LAK TKroesen NK Platforms Live cell stain(ex/em*) Dead cell stain(ex/em*) LIVE/DEAD Cell-Mediated Cytotoxicity Kit I, FC DiOC18 (484/501) PI (535/617) 2,000 assays L7010 * Excitation (Ex) and emission (Em) maxima, in nm. I = Imaging.. FC = Flow cytometry. LIVE/DEAD SYBR 14 LIVE/DEAD 5 10 DNA UV LIVE/DEAD SYBR 14( ) ( ) ( ) Duane L. Garner USDA( ) Lawrence A. Johnson Platforms Live cell stain(ex/em*) Dead cell stain(ex/em*) LIVE/DEAD Sperm Viability Kit I, FC SYBR 14 (484/516) PI (535/617) 200 1,000 assays L7011 * Excitation (Ex) and emission (Em) maxima, in nm. I = Imaging.. FC = Flow cytometry. 119
23 / LIVE/DEAD LIVE/DEAD Cell Vitality Assay Kit 2 C12- C12- SYTOX Green LIVE/DEAD Jurkat T Platforms Live cell stain(ex/em*) Dead cell stain(ex/em*) LIVE/DEAD Cell Vitality Assay Kit FC C12-resazurin (563/587) SYTOX Green (504/523) 1,000 assays L34951 * Excitation (Ex) and emission (Em) maxima, in nm. FC = Flow cytometry. Vybrant Vybrant C12- C12- Vybrant C12- Jurkat C12- / 530/590 nm alamarblue 8000 / Chapter 4 Platforms Live cell stain(ex/em*) Dead cell stain(ex/em*) Vybrant Cell Metabolic Assay Kit MP, FC C12-resazurin (563/587) None 200 1,000 assays V23110 * Excitation (Ex) and emission (Em) maxima, in nm.. MP = Microplate. FC = Flow cytometry
24 / ph phrodo BioParticles GFP fluo-4 phrodo ph ON OFF Molecular Probes ph phrodo ph phrodo phrodo E.coli BioParticles phrodo SE phrodo ph phrodo SE ph 7.4 ph ph ph ~5 18 phrodo phrodo phrodo ph phrodo 19 phrodo E.coli 189
25 Side scatter Counts / phrodo phrodo phrodo phrodo E.coli BioParticles phrodo SE phrodo 96 A 20 phrodo E. coli BioParticles J774A1 phrodo E. coli BioParticles 2 Lucy Deriy DeborahNelson B 1, R C , Forward scatter (A) (B) phrodo phrodo E.coli 100 l phrodo E.coli nm nm FACSCalibur BD Biosciences A. B. 37 phrodo E.coli phrodo E.coli A E.coli B C J774 3 phrodo Chapter 4 Platform Laser (nm) Ex/Em phrodo Dextran, 10,000 MW for endocytosis I, HCS 488, / mg P10361 phrodo, Succinimidyl Ester (phrodo, SE) FC, I, MP /585 1 mg P36600 phrodo E. coli BioParticles Conjugate for Phagocytosis FC, I, MP /585 5 x 2 mg P35361 phrodo S. aureus BioParticles Conjugate for Phagocytosis FC, I, MP /585 5 x 2 mg A10010 phrodo E. coli BioParticles Phagocytosis Kit for flow cytometry FC / assays A10025 phrodo Phagocytosis Particle Labeling Kit for flow cytometry FC / assays A10026 FC = flow cytometry; HCS = high-content screening; I = imaging; MP = microplate; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm
26 / ( ) Invitrogen Fc OxyBURST Green ( ) Fc OxyBURST Green (H 2 DCF) (BSA) BSA BSA Fc Fc H 2 DCF Fluorescence Control CGD Time (seconds) Fc Fc OxyBURST (H 2 DCF) BSA BSA Fc DCF CGD Fc OxyBURST Boston University Elizabeth Simons Platforms Laser (nm) Ex/Em Fc OxyBURST Green assay reagent MP, FC / assays F2902 FC = flow cytometry; MP = microplate; Ex/Em = excitation (Ex) and emission (Em) maxima, in nm. 122
27 価格表 ( ページ順 ) ページカタログ番号製品名 サイズ 価格 ( ) 93 A13199 annexin V, fluorescein conjugate (FITC annexin V) *100 assays* 500 UL 54, A13201 annexin V, Alexa Fluor 488 conjugate *100 assays* 500 UL 68, A13202 annexin V, Alexa Fluor 568 conjugate *100 assays* 500 UL 68, A13203 annexin V, Alexa Fluor 594 conjugate *100 assays* 500 UL 68, A23202 annexin V, Alexa Fluor 350 conjugate *100 assays* 500 UL 68, A23204 annexin V, Alexa Fluor 647 conjugate *100 assays* 500 UL 68, A35108 annexin V, Alexa Fluor 555 conjugate *100 assays* 500 UL 68, A35110 annexin V, allophycocyanin conjugate (APC annexin V) *50 assays* 250 UL 68, A35111 annexin V, R-phycoerythrin conjugate (R-PE annexin V) *50 assays* 250 UL 68, A35122 annexin V, Pacific Blue conjugate *for flow cytometry* *100 assays* 500 UL 68, A35137 Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit *for flow cytometry* *100 assays* 1 KIT 48, P3581 PO-PRO-1 iodide (435/455) *1 mm solution in DMSO* 1 ML 54, T3605 TO-PRO-3 iodide (642/661) *1 mm solution in DMSO* 1 ML 53, Y3603 YO-PRO-1 iodide (491/509) *1 mm solution in DMSO* 1 ML 53, D23806 dihydrorhodamine 123 *5 mm stabilized solution in DMSO* 1 ML 40, H10295 HCS Mitochondrial Health Kit *2-plate size* 1 KIT 39, I35103 Image-iT LIVE Mitochondrial Transition Pore Assay Kit *for microscopy* 1 KIT 98, M34150 MitoProbe DiOC 2(3) assay kit 1 KIT 43, M34151 MitoProbe DilC 1(15) assay kit 1 KIT 43, M34152 MitoProbe JC-1 assay kit 1 KIT 85, M34153 MitoProbe Transition Pore assay kit 1 KIT 85, M7510 MitoTracker Orange CMTMRos *special packaging* 20 X 50 UG 43, M7511 MitoTracker Orange CM-H2TMRos *special packaging* 20 X 50 UG 52, M7512 MitoTracker Red CMXRos *special packaging* 20 X 50 UG 43, M7513 MitoTracker Red CM-H2XRos *special packaging* 20 X 50 UG 52, M7514 MitoTracker Green FM *special packaging* 20 X 50 UG 43, T3168 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1; CBIC2(3)) 5 MG 75, T668 tetramethylrhodamine, methyl ester, perchlorate (TMRM) 25 MG 28, T669 tetramethylrhodamine, ethyl ester, perchlorate (TMRE) 25 MG 28, I35101 Image-iT LIVE Red Poly Caspases Detection Kit *for microscopy* 1 KIT 54, I35102 Image-iT LIVE Red Caspase-3 and -7 Detection Kit *for microscopy* 1 KIT 59, I35104 Image-iT LIVE Green Poly Caspases Detection Kit *for microscopy* *25 tests* 1 KIT 37, I35105 Image-iT LIVE Green Caspase-8 Detection Kit *for microscopy* *25 tests* 1 KIT 37, I35106 Image-iT LIVE Green Caspase-3 and -7 Detection Kit *for microscopy* *25 tests* 1 KIT 37, V35117 Vybrant FAM Poly Caspases Assay Kit *for flow cytometry* *25 assays* 1 KIT 37, V35118 Vybrant FAM Caspase-3 and -7 assay kit 1 KIT 37, V35119 Vybrant FAM Caspase-8 assay kit 1 KIT 37, A35135 Vybrant DyeCycle Violet/SYTOX AADvanced Apoptosis Kit *for chromatin condensation* *for flow cytometry* *200 assays* 1 KIT 37, H3570 Hoechst *10 mg/ml solution in water* 10 ML 16, P3566 propidium iodide *1.0 mg/ml solution in water* 10 ML 11, V V23201 Chromatin Condensation/Dead Cell Apoptosis Kit *Hoechst 33342/propidium iodide* *200 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #5 *Hoechst 33342/propidium iodide* *200 assays*) Chromatin Condesation/Membrane Permeability/Dead Cell Apoptosis Kit. *Hoechst 33342/YO-PRO -1/propidium iodide* *200 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #7 *Hoechst 33342/YO-PRO-1/propidium iodide* *200 assays*) 1 KIT 31,900 1 KIT 50, V35003 Vybrant DyeCycle Violet stain *5 mm in water* *200 assays* 200 UL 34, A23210 APO-BrdU TUNEL Assay Kit *with Alexa Fluor 488 anti-brdu* *60 assays* PROBES 121, C10245 Click-iT TUNEL Alexa Fluor 488 Imaging Assay *for microscopy and HCS* * assays* 1 KIT 102, C10246 Click-iT TUNEL Alexa Fluor 594 Imaging Assay *for microscopy and HCS* * assays* 1 KIT 102, C10247 Click-iT TUNEL Alexa Fluor 647 Imaging Assay *for microscopy and HCS* * assays* 1 KIT 102, KHO1001 APO BRDU (TUNEL) KIT 60 TESTS 82, A35135 Vybrant DyeCycle Violet/SYTOX AADvanced Apoptosis Kit *for chromatin condensation* *for flow cytometry* *200 assays* 1 KIT 37, A35136 Pacific Blue Annexin V/SYTOX AADvanced Apoptosis Kit *for flow cytometry* *50 assays* 1 KIT 56, V13240 Single Channel Annexin V/ Dead Cell Apoptosis Kit *Alexa Fluor 488 annexin V/SYTOX Green* *50 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #1 *Alexa Fluor 488 annexin V/SYTOX Green* *50 assays*) 1 KIT 63,000 Immunology Catalog には抗体 (4 章 ) 二次検出と抗体標識 (5 章 ) コントロールとスタンダード (7 章 ) もでています Immunology Catalog ( 英文 ) は次のサイトでご覧いただけます
28 価格表 ( ページ順 ) ページカタログ番号製品名サイズ価格 ( ) 100 V V V V V V V V35114 Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit *Alexa Fluor 488 annexin V/propidium iodide* *50 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #2 *Alexa Fluor 488 annexin V/propidium iodide* *50 assays*) FITC Annexin V/ Dead Cell Apoptosis Kit *FITC annexin V/propidium iodide* *50 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #3 *FITC annexin V/propidium iodide* *50 assays*) Membrane Permeability/ Dead Cell Apoptosis Kit *YO-PRO -1/propidium iodide* *200 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #4 *YO-PRO-1/propidium iodide* *200 assays*) Chromatin Condensation/Dead Cell Apoptosis Kit *Hoechst 33342/propidium iodide* *200 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #5 *Hoechst 33342/propidium iodide* *200 assays*) Chromatin Condesation/Membrane Permeability/Dead Cell Apoptosis Kit. *Hoechst 33342/YO-PRO -1/propidium iodide* *200 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #7 *Hoechst 33342/YO-PRO-1/propidium iodide* *200 assays*) PE Annexin V/ Dead Cell Apoptosis Kit *with SYTOX Green* *50 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #8 *R-phycoerythrin annexin V/SYTOX Green* *50 assays*) APC Annexin V/Dead Cell Apoptosis Kit *with APC annexin V and SYTOX Green* *50 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #9 *allophycocyanin annexin V/SYTOX Green* *50 assays*) Metabolic Activity/Annexin V/Dead Cell Apoptosis Kit *with C12 resazurin, APC annexin V, and SYTOX Green* *50 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #10 *allophycocyanin annexin V/C12-resazurin/SYTOX Green* *50 assays*) 1 KIT 32,000 1 KIT 50,100 1 KIT 42,000 1 KIT 31,900 1 KIT 50,100 1 KIT 113,100 1 KIT 113,100 1 KIT 122, V V35123 Mitochondrial Membrane Potential/Annexin V Apoptosis Kit *Alexa Fluor 488 annexin V/MitoTracker Red CMXRos* *50 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #11 *Alexa Fluor 488 annexin V/MitoTracker Red CMXRos* *50 assays*) Violet Membrane Permeability/Dead Cell Apoptosis Kit *with PO-PRO -1 and 7- aminoactinomycin D* *200 assays* *for flow cytometry* ( 旧名 Vybrant Apoptosis Assay Kit #13 *PO-PRO-1/7-aminoactinomycin D* *for flow cytometry* *200 assays*) 1 KIT 66,400 1 KIT 47, F10347 FxCycle Violet stain 1 SET 48, F10348 FxCycle Far Red stain 1 SET 48, V10273 Vybrant DyeCycle Ruby stain *2.5 mm solution in DMSO* *400 assays* 400 UL 53, V10309 Vybrant DyeCycle Ruby stain *2.5 mm solution in DMSO* *100 assays* 100 UL 29, V35003 Vybrant DyeCycle Violet stain *5 mm in water* *200 assays* 200 UL 34, V35004 Vybrant DyeCycle Green stain *5 mm solution in DMSO* *200 assays* 400 UL 34, V35005 Vybrant DyeCycle Orange stain *5 mm solution in DMSO* *200 assays* 400 UL 34, C35006 CyQUANT NF Cell Proliferation Assay Kit *1000 assays* 1 KIT 56, C35007 CyQUANT NF Cell Proliferation Assay Kit *200 assays* 1 KIT 22, C35011 CyQUANT Direct Cell Proliferation Assay Kit *for 10 microplates* 1 KIT 38, C35012 CyQUANT Direct Cell Proliferation Assay Kit *for 100 microplates* 1 KIT 270, C7026 CyQUANT Cell Proliferation Assay Kit *for cells in culture* *1000 assays* 1 KIT 60, C7027 CyQUANT cell lysis buffer *20X concentrate* 50 ML 20, DAL1025 ALAMAR BLUE 25 ML 20, DAL1100 ALAMAR BLUE 100 ML 52, M6494 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) 1 G 16, V13154 Vybrant MTT Cell Proliferation Assay Kit *1000 assays* 1 KIT 39, A10034 Click-iT EdU Pacific Blue Flow Cytometory Assay Kit 1 KIT 82, A10044 EdU (5-ethynyl-2'-deoxyuridine) *50 mg* 50 MG 53, A10202 Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit *50 assays* 1 KIT 82, C10214 Click-iT EdU Microplate Assay *400 assays* 1 KIT 56, C10337 Click-iT EdU Alexa Fluor 488 Imaging Kit *for 50 coverslips* 1 KIT 73, C10339 Click-iT EdU Alexa Fluor 594 Imaging Kit *for 50 coverslips* 1 KIT 73, C10340 Click-iT EdU Alexa Fluor 647 Imaging Kit *for 50 coverslips* 1 KIT 73, C10350 Click-iT EdU Alexa Fluor 488 HCS Assay *2-plate size* 1 KIT 71, C10351 Click-iT EdU Alexa Fluor 488 HCS Assay *10-plate size* 1 KIT 288, C10352 Click-iT EdU Alexa Fluor 555 HCS Assay *2-plate size* 1 KIT 71, C10353 Click-iT EdU Alexa Fluor 555 HCS Assay *10-plate size* 1 KIT 288, C10354 Click-iT EdU Alexa Fluor 594 HCS Assay *2-plate size* 1 KIT 71, C10355 Click-iT EdU Alexa Fluor 594 HCS Assay *10-plate size* 1 KIT 288, C10356 Click-iT EdU Alexa Fluor 647 HCS Assay *2-plate size* 1 KIT 71, C10357 Click-iT EdU Alexa Fluor 647 HCS Assay *10-plate size* 1 KIT 288, C35002 Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit *50 assays* 1 KIT 82,800 Immunology Catalog には抗体 (4 章 ) 二次検出と抗体標識 (5 章 ) コントロールとスタンダード (7 章 ) もでています Immunology Catalog ( 英文 ) は次のサイトでご覧いただけます
29 価格表 ( ページ順 ) ページカタログ番号製品名 サイズ 価格 ( ) 110 E10187 EdU (5-ethynyl-2'-deoxyuridine) *500 mg* 500 MG 163, C34554 CellTrace CFSE Cell Proliferation Kit *for flow cytometry* 1 KIT 28, C34557 CellTrace Violet Cell Proliferation Kit *for flow cytometry* 1 KIT 58, A10346 ArC Bead Kit 1 KIT 37, L10119 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit *for 633 or 635 nm excitation* *200 assays* 1 KIT 45, L10120 LIVE/DEAD Fixable Far Red Dead Cell Stain Kit *for 633 or 635 nm excitation* *200 assays* 1 KIT 45, L23101 LIVE/DEAD Fixable Green Dead Cell Stain Kit *for 488 nm excitation* *200 assays* 1 KIT 46, L23102 LIVE/DEAD Fixable Red Dead Cell Stain Kit *for 488 nm excitation* *200 assays* 1 KIT 46, L23105 LIVE/DEAD Fixable Blue Dead Cell Stain Kit *for UV excitation* *200 assays* 1 KIT 46, L34955 LIVE/DEAD Fixable Violet Dead Cell Stain Kit *for 405 nm excitation* *200 assays* 1 KIT 46, L34957 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit *for 405 nm excitation* *200 assays* 1 KIT 45, L34959 LIVE/DEAD Fixable Yellow Dead Cell Stain Kit *for 405 nm excitation* *200 assays* 1 KIT 45, L34960 LIVE/DEAD Fixable Dead Cell Stain Sampler Kit 1 KIT 48, S10274 SYTOX AADvanced dead cell stain *for 488 nm excitation* *500 assays* 1 KIT 48, S10349 SYTOX AADvanced dead cell stain *for 488 nm excitation* *100 assays* 1 KIT 15, S11348 SYTOX Blue nucleic acid stain *5 mm solution in DMSO* 250 UL 42, S11368 SYTOX Orange nucleic acid stain *5 mm solution in DMSO* 250 UL 42, S34859 SYTOX Red dead cell stain *for 633 or 635 nm excitation* *5 um solution in DMSO* 1 ML. 55, S7020 SYTOX Green nucleic acid stain *5 mm solution in DMSO* 250 UL 42, C34851 CellTrace calcein red-orange, AM *special packaging* 20 X 50 UG 54, C34852 CellTrace calcein green, AM *special packaging* 20 X 50 UG 54, C34853 CellTrace calcein blue, AM *special packaging* 20 X 50 UG 54, C34858 CellTrace calcein Violet, AM 20 X 25 UG 55, L3224 LIVE/DEAD Viability/Cytotoxicity Kit *for animal cells* *1000 assays* 1 KIT 75, L34958 LIVE/DEAD Violet Viability/Vitality Kit *for 405 nm excitation* *200 assays* 1 KIT 75, L7010 LIVE/DEAD Cell-Mediated Cytotoxicity Kit *for animal cells* *2000 assays* 1 KIT 40, L7011 LIVE/DEAD Sperm Viability Kit * assays* 1 KIT 75, L7013 LIVE/DEAD Reduced Biohazard Cell Viability Kit #1 *green and red fluorescence* *100 assays* 1 KIT 40, L34951 LIVE/DEAD Cell Vitality Assay Kit *C12-resazurin/SYTOX Green* *1000 assays* 1 KIT 54, V23110 Vybrant Cell Metabolic Assay Kit *with C12-resazurin* * assays* 1 KIT 46, A10010 phrodo S. aureus BioParticles Conjugates for Phagocytosis 5 X 2 MG. 52, A10025 phrodo E. coli BioParticles Phagocytosis Kit for flow cytometry 1 KIT 142, A10026 phrodo Phagocytosis Particles Labeling Kit for flow cytometry 1 KIT 142, P10361 dextran, phrodo 10,000 MW for endocytosis 0.5 MG 42, P35361 phrodo E. coli BioParticles conjugate for phagocytosis 5 X 2 MG 52, P36600 phrodo, succinimidyl ester (phrodo, SE) 1 MG 86, F2902 Fc OxyBURST Green assay reagent *25 assays* *3 mg/ml* 500 UL 49,100 Immunology Catalog には抗体 (4 章 ) 二次検出と抗体標識 (5 章 ) コントロールとスタンダード (7 章 ) もでています Immunology Catalog ( 英文 ) は次のサイトでご覧いただけます
30 価格表 ( 製品名順 ) カタログ番号製品名 サイズ 価格 ( ) ページ T3168 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1; CBIC2(3)) 5 MG 75, DAL1025 ALAMAR BLUE 25 ML 20, DAL1100 ALAMAR BLUE 100 ML 52, V13241 Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit *Alexa Fluor 488 annexin V/propidium iodide* *50 assays* *for flow cytometry* 1 KIT A23202 annexin V, Alexa Fluor 350 conjugate *100 assays* 500 UL 68, A13201 annexin V, Alexa Fluor 488 conjugate *100 assays* 500 UL 68, A35108 annexin V, Alexa Fluor 555 conjugate *100 assays* 500 UL 68, A13202 annexin V, Alexa Fluor 568 conjugate *100 assays* 500 UL 68, A13203 annexin V, Alexa Fluor 594 conjugate *100 assays* 500 UL 68, A23204 annexin V, Alexa Fluor 647 conjugate *100 assays* 500 UL 68, A35110 annexin V, allophycocyanin conjugate (APC annexin V) *50 assays* 250 UL 68, A13199 annexin V, fluorescein conjugate (FITC annexin V) *100 assays* 500 UL 54, A35122 annexin V, Pacific Blue conjugate *for flow cytometry* *100 assays* 500 UL 68, A35111 annexin V, R-phycoerythrin conjugate (R-PE annexin V) *50 assays* 250 UL 68, V35113 APC Annexin V/Dead Cell Apoptosis Kit *with APC annexin V and SYTOX Green* *50 assays* *for flow cytometry* 1 KIT KHO1001 APO BRDU (TUNEL) KIT 60 TESTS 82, A23210 APO-BrdU TUNEL Assay Kit *with Alexa Fluor 488 anti-brdu* *60 assays* PROBES 121, A10346 ArC Bead Kit 1 KIT 37, C34853 CellTrace calcein blue, AM *special packaging* 20 X 50 UG 54, C34852 CellTrace calcein green, AM *special packaging* 20 X 50 UG 54, C34851 CellTrace calcein red-orange, AM *special packaging* 20 X 50 UG 54, C34858 CellTrace calcein Violet, AM 20 X 25 UG 55, C34554 CellTrace CFSE Cell Proliferation Kit *for flow cytometry* 1 KIT 28, C34557 CellTrace Violet Cell Proliferation Kit *for flow cytometry* 1 KIT 58, V13244 Chromatin Condensation/Dead Cell Apoptosis Kit *Hoechst 33342/propidium iodide* *200 assays* *for flow cytometry* 1 KIT V23201 Chromatin Condesation/Membrane Permeability/Dead Cell Apoptosis Kit. *Hoechst 33342/YO-PRO -1/propidium iodide* *200 assays* *for flow cytometry* 1 KIT C35002 Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit *50 assays* 1 KIT 82, C10350 Click-iT EdU Alexa Fluor 488 HCS Assay *2-plate size* 1 KIT 71, C10351 Click-iT EdU Alexa Fluor 488 HCS Assay *10-plate size* 1 KIT 288, C10337 Click-iT EdU Alexa Fluor 488 Imaging Kit *for 50 coverslips* 1 KIT 73, C10353 Click-iT EdU Alexa Fluor 555 HCS Assay *10-plate size* 1 KIT 288, C10352 Click-iT EdU Alexa Fluor 555 HCS Assay *2-plate size* 1 KIT 71, C10354 Click-iT EdU Alexa Fluor 594 HCS Assay *2-plate size* 1 KIT 71, C10355 Click-iT EdU Alexa Fluor 594 HCS Assay *10-plate size* 1 KIT 288, C10339 Click-iT EdU Alexa Fluor 594 Imaging Kit *for 50 coverslips* 1 KIT 73, A10202 Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit *50 assays* 1 KIT 82, C10356 Click-iT EdU Alexa Fluor 647 HCS Assay *2-plate size* 1 KIT 71, C10357 Click-iT EdU Alexa Fluor 647 HCS Assay *10-plate size* 1 KIT 288, C10340 Click-iT EdU Alexa Fluor 647 Imaging Kit *for 50 coverslips* 1 KIT 73, C10214 Click-iT EdU Microplate Assay *400 assays* 1 KIT 56, A10034 Click-iT EdU Pacific Blue Flow Cytometory Assay Kit 1 KIT 82, C10245 Click-iT TUNEL Alexa Fluor 488 Imaging Assay *for microscopy and HCS* * assays* 1 KIT 102, C10246 Click-iT TUNEL Alexa Fluor 594 Imaging Assay *for microscopy and HCS* * assays* 1 KIT 102, C10247 Click-iT TUNEL Alexa Fluor 647 Imaging Assay *for microscopy and HCS* * assays* 1 KIT 102, C7027 CyQUANT cell lysis buffer *20X concentrate* 50 ML 20, C7026 CyQUANT Cell Proliferation Assay Kit *for cells in culture* *1000 assays* 1 KIT 60, C35011 CyQUANT Direct Cell Proliferation Assay Kit *for 10 microplates* 1 KIT 38, C35012 CyQUANT Direct Cell Proliferation Assay Kit *for 100 microplates* 1 KIT 270, C35006 CyQUANT NF Cell Proliferation Assay Kit *1000 assays* 1 KIT 56, C35007 CyQUANT NF Cell Proliferation Assay Kit *200 assays* 1 KIT 22, P10361 dextran, phrodo 10,000 MW for endocytosis 0.5 MG 42, D23806 dihydrorhodamine 123 *5 mm stabilized solution in DMSO* 1 ML 40, A10044 EdU (5-ethynyl-2'-deoxyuridine) *50 mg* 50 MG 53, E10187 EdU (5-ethynyl-2'-deoxyuridine) *500 mg* 500 MG 163, F2902 Fc OxyBURST Green assay reagent *25 assays* *3 mg/ml* 500 UL 49, V13242 FITC Annexin V/ Dead Cell Apoptosis Kit *FITC annexin V/propidium iodide* *50 assays* *for flow cytometry* 1 KIT F10348 FxCycle Far Red stain 1 SET 48, F10347 FxCycle Violet stain 1 SET 48, Immunology Catalog には抗体 (4 章 ) 二次検出と抗体標識 (5 章 ) コントロールとスタンダード (7 章 ) もでています Immunology Catalog ( 英文 ) は次のサイトでご覧いただけます
31 価格表 ( 製品名順 ) カタログ番号製品名サイズ価格 ( ) ページ H10295 HCS Mitochondrial Health Kit *2-plate size* 1 KIT 39, H3570 Hoechst *10 mg/ml solution in water* 10 ML 16, I35106 Image-iT LIVE Green Caspase-3 and -7 Detection Kit *for microscopy* *25 tests* 1 KIT 37, I35105 Image-iT LIVE Green Caspase-8 Detection Kit *for microscopy* *25 tests* 1 KIT 37, I35104 Image-iT LIVE Green Poly Caspases Detection Kit *for microscopy* *25 tests* 1 KIT 37, I35103 Image-iT LIVE Mitochondrial Transition Pore Assay Kit *for microscopy* 1 KIT 98, I35102 Image-iT LIVE Red Caspase-3 and -7 Detection Kit *for microscopy* 1 KIT 59, I35101 Image-iT LIVE Red Poly Caspases Detection Kit *for microscopy* 1 KIT 54, L34951 LIVE/DEAD Cell Vitality Assay Kit *C12-resazurin/SYTOX Green* *1000 assays* 1 KIT 54, L7010 LIVE/DEAD Cell-Mediated Cytotoxicity Kit *for animal cells* *2000 assays* 1 KIT 40, L34957 LIVE/DEAD Fixable Aqua Dead Cell Stain Kit *for 405 nm excitation* *200 assays* 1 KIT 45, L23105 LIVE/DEAD Fixable Blue Dead Cell Stain Kit *for UV excitation* *200 assays* 1 KIT 46, L34960 LIVE/DEAD Fixable Dead Cell Stain Sampler Kit 1 KIT 48, L10120 LIVE/DEAD Fixable Far Red Dead Cell Stain Kit *for 633 or 635 nm excitation* *200 assays* 1 KIT 45, L23101 LIVE/DEAD Fixable Green Dead Cell Stain Kit *for 488 nm excitation* *200 assays* 1 KIT 46, L10119 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit *for 633 or 635 nm excitation* *200 assays* 1 KIT 45, L23102 LIVE/DEAD Fixable Red Dead Cell Stain Kit *for 488 nm excitation* *200 assays* 1 KIT 46, L34955 LIVE/DEAD Fixable Violet Dead Cell Stain Kit *for 405 nm excitation* *200 assays* 1 KIT 46, L34959 LIVE/DEAD Fixable Yellow Dead Cell Stain Kit *for 405 nm excitation* *200 assays* 1 KIT 45, L7013 LIVE/DEAD Reduced Biohazard Cell Viability Kit #1 *green and red fluorescence* *100 assays* 1 KIT 40, L7011 LIVE/DEAD Sperm Viability Kit * assays* 1 KIT 75, L3224 LIVE/DEAD Viability/Cytotoxicity Kit *for animal cells* *1000 assays* 1 KIT 75, L34958 LIVE/DEAD Violet Viability/Vitality Kit *for 405 nm excitation* *200 assays* 1 KIT 75, V13243 Membrane Permeability/ Dead Cell Apoptosis Kit *YO-PRO -1/propidium iodide* *200 assays* *for flow cytometry* 1 KIT V35114 Metabolic Activity/Annexin V/Dead Cell Apoptosis Kit *with C12 resazurin, APC annexin V, and SYTOX Green* *50 assays* *for flow cytometry* 1 KIT V35116 Mitochondrial Membrane Potential/Annexin V Apoptosis Kit *Alexa Fluor 488 annexin V/MitoTracker Red CMXRos* *50 assays* *for flow cytometry* 1 KIT M34151 MitoProbe DilC 1(15) assay kit 1 KIT 43, M34150 MitoProbe DiOC 2(3) assay kit 1 KIT 43, M34152 MitoProbe JC-1 assay kit 1 KIT 85, M34153 MitoProbe Transition Pore assay kit 1 KIT 85, M7514 MitoTracker Green FM *special packaging* 20 X 50 UG 43, M7511 MitoTracker Orange CM-H2TMRos *special packaging* 20 X 50 UG 52, M7510 MitoTracker Orange CMTMRos *special packaging* 20 X 50 UG 43, M7513 MitoTracker Red CM-H2XRos *special packaging* 20 X 50 UG 52, M7512 MitoTracker Red CMXRos *special packaging* 20 X 50 UG 43, M6494 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) 1 G 16, A35136 Pacific Blue Annexin V/SYTOX AADvanced Apoptosis Kit *for flow cytometry* *50 assays* 1 KIT 56, V35112 PE Annexin V/ Dead Cell Apoptosis Kit *with SYTOX Green* *50 assays* *for flow cytometry* 1 KIT P35361 phrodo E. coli BioParticles conjugate for phagocytosis 5 X 2 MG 52, A10025 phrodo E. coli BioParticles Phagocytosis Kit for flow cytometry 1 KIT 142, A10026 phrodo Phagocytosis Particles Labeling Kit for flow cytometry 1 KIT 142, A10010 phrodo S. aureus BioParticles Conjugates for Phagocytosis 5 X 2 MG. 52, P36600 phrodo, succinimidyl ester (phrodo, SE) 1 MG 86, P3581 PO-PRO-1 iodide (435/455) *1 mm solution in DMSO* 1 ML 54, P3566 propidium iodide *1.0 mg/ml solution in water* 10 ML 11, V13240 Single Channel Annexin V/ Dead Cell Apoptosis Kit *Alexa Fluor 488 annexin V/SYTOX Green* *50 assays* *for flow cytometry* 1 KIT S10349 SYTOX AADvanced dead cell stain *for 488 nm excitation* *100 assays* 1 KIT 15, S10274 SYTOX AADvanced dead cell stain *for 488 nm excitation* *500 assays* 1 KIT 48, S11348 SYTOX Blue nucleic acid stain *5 mm solution in DMSO* 250 UL 42, S7020 SYTOX Green nucleic acid stain *5 mm solution in DMSO* 250 UL 42, S11368 SYTOX Orange nucleic acid stain *5 mm solution in DMSO* 250 UL 42, S34859 SYTOX Red dead cell stain *for 633 or 635 nm excitation* *5 um solution in DMSO* 1 ML. 55, T669 tetramethylrhodamine, ethyl ester, perchlorate (TMRE) 25 MG 28, T668 tetramethylrhodamine, methyl ester, perchlorate (TMRM) 25 MG 28, T3605 TO-PRO-3 iodide (642/661) *1 mm solution in DMSO* 1 ML 53, A35136 Violet Annexin V/Dead Cell Apoptosis Kit *Pacific Blue annexin V/SYTOX AADvanced * *for flow cytometry* *50 assays* 1 KIT Immunology Catalog には抗体 (4 章 ) 二次検出と抗体標識 (5 章 ) コントロールとスタンダード (7 章 ) もでています Immunology Catalog ( 英文 ) は次のサイトでご覧いただけます
32 価格表 ( 製品名順 ) カタログ番号製品名サイズ価格 ( ) ページ A35135 Violet Chromatin Condensation/Dead Cell Apoptosis Kit *Vybrant DyeCycle Violet and SYTOX AADvanced * *for flow cytometry* *200 assays* 1 KIT V35123 Violet Membrane Permeability/Dead Cell Apoptosis Kit *with PO-PRO -1 and 7- aminoactinomycin D* *200 assays* *for flow cytometry* 1 KIT A35137 Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit *for flow cytometry* *100 assays* 1 KIT 48, A35137 Violet Ratiometric Membrane Asymmetry Probe/Dead Cell Apoptosis Kit *for flow cytometry* *100 assays* 1 KIT V13240 Vybrant Apoptosis Assay Kit #1 *Alexa Fluor 488 annexin V/SYTOX Green* *50 assays* 1 KIT 63, V35114 Vybrant Apoptosis Assay Kit #10 *allophycocyanin annexin V/C12-resazurin/SYTOX Green* *50 assays* 1 KIT 122, V35116 Vybrant Apoptosis Assay Kit #11 *Alexa Fluor 488 annexin V/MitoTracker Red CMXRos* *50 assays* 1 KIT 66, V35123 Vybrant Apoptosis Assay Kit #13 *PO-PRO-1/7-aminoactinomycin D* *for flow cytometry* *200 assays* 1 KIT 47, V13241 Vybrant Apoptosis Assay Kit #2 *Alexa Fluor 488 annexin V/propidium iodide* *50 assays* 1 KIT 32, V13242 Vybrant Apoptosis Assay Kit #3 *FITC annexin V/propidium iodide* *50 assays* 1 KIT 50, V13243 Vybrant Apoptosis Assay Kit #4 *YO-PRO-1/propidium iodide* *200 assays* 1 KIT 42, V13244 Vybrant Apoptosis Assay Kit #5 *Hoechst 33342/propidium iodide* *200 assays* 1 KIT 31, V13244 Vybrant Apoptosis Assay Kit #5 *Hoechst 33342/propidium iodide* *200 assays* 1 KIT 31, V23201 Vybrant Apoptosis Assay Kit #7 *Hoechst 33342/YO-PRO-1/propidium iodide* *200 assays* 1 KIT 50, V23201 Vybrant Apoptosis Assay Kit #7 *Hoechst 33342/YO-PRO-1/propidium iodide* *200 assays* 1 KIT 50, V35112 Vybrant Apoptosis Assay Kit #8 *R-phycoerythrin annexin V/SYTOX Green* *50 assays* 1 KIT 113, V35113 Vybrant Apoptosis Assay Kit #9 *allophycocyanin annexin V/SYTOX Green* *50 assays* 1 KIT 113, V23110 Vybrant Cell Metabolic Assay Kit *with C12-resazurin* * assays* 1 KIT 46, V35004 Vybrant DyeCycle Green stain *5 mm solution in DMSO* *200 assays* 400 UL 34, V35005 Vybrant DyeCycle Orange stain *5 mm solution in DMSO* *200 assays* 400 UL 34, V10309 Vybrant DyeCycle Ruby stain *2.5 mm solution in DMSO* *100 assays* 100 UL 29, V10273 Vybrant DyeCycle Ruby stain *2.5 mm solution in DMSO* *400 assays* 400 UL 53, V35003 Vybrant DyeCycle Violet stain *5 mm in water* *200 assays* 200 UL 34, V35003 Vybrant DyeCycle Violet stain *5 mm in water* *200 assays* 200 UL 34, A35135 Vybrant DyeCycle Violet/SYTOX AADvanced Apoptosis Kit *for chromatin condensation* *for flow cytometry* *200 assays* 1 KIT 37, A35135 Vybrant DyeCycle Violet/SYTOX AADvanced Apoptosis Kit *for chromatin condensation* *for flow cytometry* *200 assays* 1 KIT 37, V35118 Vybrant FAM Caspase-3 and -7 assay kit 1 KIT 37, V35119 Vybrant FAM Caspase-8 assay kit 1 KIT 37, V35117 Vybrant FAM Poly Caspases Assay Kit *for flow cytometry* *25 assays* 1 KIT 37, V13154 Vybrant MTT Cell Proliferation Assay Kit *1000 assays* 1 KIT 39, Y3603 YO-PRO-1 iodide (491/509) *1 mm solution in DMSO* 1 ML 53, Immunology Catalog には抗体 (4 章 ) 二次検出と抗体標識 (5 章 ) コントロールとスタンダード (7 章 ) もでています Immunology Catalog ( 英文 ) は次のサイトでご覧いただけます
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369 384 368 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website www.lifetechnologies.com). By use of these
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E-mail: khatano@fms.saitama-u.ac.jp Tel & Fax: 048-858-3535 Toxin Virus Bacterium Glycolipid Glycoprotein Vero toxin (Stx1 and Stx2) Side view Bottom view H H H H N H Grobotriaosyl Ceramide (Gb 3 : Galα1-4Galβ1-4Glcβ-Cer)
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