Table 1 Bacteriae to be tested (1) Gram positive cocci Coagulase positive staphylococcus Coagulase negative staphylococcus Streptococcus pneumoniae Gr

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1 Chlamydia trachomatis Key words: chlamydial antigen, ELISA, bacteria, cross reaction, respiratory infection C. trachomatis Branhamella catarrhalis C. trachomatis C. trachomatis Chlamydia trachomatis (C. trachomatis) C. tracho- matis Neisseria Branhamella catarrhalis (B. catarrhalis) Haemophilus

2 Table 1 Bacteriae to be tested (1) Gram positive cocci Coagulase positive staphylococcus Coagulase negative staphylococcus Streptococcus pneumoniae Group A streptococcus (2) Gram negative cocci Branhamella catarrhalis Neisseria mucosa Neisseria sicca Neisseria lactamica (3) Gram negative rods Escherichia coli Haemophilus influenzae Pseudomonas aeruginosa Fig. 1-1 Assay procedure of IDEIA Mark III

3 Cklamydia Fig. 1-2 Positive samples trachomatis抗 原 検 査 の 気 道常 在 細 菌 との交 叉反 応 of IDEIA Mark HI 1197 な か っ た 最 大 濃 度 をTable2に 球 菌 に はChlamydiazyme陽 ら れ な か っ た が,グ ラ ム陽 性 ラ ム陰 性 菌 に お い て は 検 討 し た16株 中10株(=62 5%)に た.ま た,グ 陽 性 の 結 果 が 得 られ ラ ム 陰 性 菌 の 菌 種 と し て は7種 種(;71.4%)に り,特 示 す.グ 性 を呈 した 菌 株 は み 中5 交 叉 反 応 が 認 め られ た こ とに な にNeisseria属 やBranhamella catarhalis に 対 す る 交 叉 反 応 の 陽 性 率 が 高 い CFU/ m1の 比 較 的 低 濃 度 で 交 叉 反 応 の み られ た 菌 株 も 存 在 し た. Table3にIDEIA Mark の 交 叉 反 応 を 示 す.グ IDEIA を,各 Mark 々Fig.1-1, IIIに よ る 測 定 手 順 お よ び 陽 性 例 1-2に 3.結 示 す. ラ ム陽 性 菌 で は コ ア グ ラ ー ゼ 陽 性 ブ ド ウ 球 菌(CPS)に1 108CFU/mlの 各 細 菌 の,Chlamydiazymeに おいて 交叉 反応 が 認 め られ た 最 小濃 度 また は 交 叉 反 応 の 認 め られ 平 成 元 年10月20日 ラ ム陰 性 菌 に つ い て は,1 高 濃 度 で も交 叉 反 応 の 認 め ら れ た 菌 は な か っ た.グ 果 Table CFU/mlの IIIに つ い て の 各 細 菌 2 Cross reactivity で 陽 性 を 呈 し た も の が1株 み ら れ た.他 濃度 の グラム 陽 性 菌 に は 交 叉 の 認 め ら れ た も の は み ら れ ず,同 of bacteriae for Chlamydiazyme

4 Table 3 Cross reactivity of bacteriae for IDEIA Mark III

5 2) Grillner, L., Beckman, S. & Hammar, H.: Comparison of two enzyme immunoassays and an immunofluorescence test for detection of Chlamydia trachomatis. Eur. J. Clin. Microbiol., 5: , ) Mohanty, K. C., O'Neill, J. J. & Hambling, M. H.: Comparison of enzyme immunoassays and cell culture for detecting Chlamydia trachomatis. Genitourin. Med., 62: , ) Krech, T., Gerhard-Fsadni, D., Hofmann, N. & Miller, S. M.: Interference of Staphylococcus aureus in the detection of Chlamydia tra- by monoclonal antibodies. Lancet, 1: chomatis , ) Saikku, P., Puolakkainen, M., Leinonen, M., Nurminen, M. & Nissinen, A.: Crossreactivity between Chlamydiazyme and Acinetobacter strains. N. Engl. J. Med., 314: , ) Taylor-Robinson, D., Thomas, B. J. & Osborn, M. F.: Evaluation of enzyme immunoassay (Chlamydiazyme) for detecting Chlamydia trachomatis in genital tract specimens. J. Clin. Pathol., 40: , ) Hammerschlag, M. R., Rettig, P. J. & Shields, M. E.: False positive results with the use of chlamydial antigen detection tests in the evaluation of suspected sexal abuse in children. Pediatr. Infect. Dis. J., 7: 11-14, ) Heggie, A. D., Lumicao, G. G., Stuart, L. A. & Gyves, M. T.: Chlamydia trachomatis infection

6 in mothers and infants. A prospective study. Am. J. Dis. Child., 135: , ) Alexander, E. R. & Harrison, H. R.: Role of Chlamydia trachomatis in perinatal Rev. Infect. Dis., 5: , infection. caused by Chlamydia trachomatis. J. Clin. Microbiol., 23: , ) Pugh, S. F., Slack, R. C. B., Caul, E. O., Paul, I. D., Appleton, P. N. & Gatley, S.: Enzyme amplified immunoassay: A novel technique applied to direct detection of Chlamydia trachomatis 14) Howard, L. V., Coleman, P. F., England, B. J. & in clinical specimens. J. Clin. Pathol., 38: , Herrmann, J. E.: Evaluation of Chlamydiazyme for the detection of genital infections Detection of Chlamydia trachomatis Antigen for Diagnosis of Respiratory Infection I. Cross Reaction with Bacteriae consisting Normal Flora Respiratory Tract Tsutomu YAMAZAKI113), So HASHIZUME2>, Hanako YOSHIZAWA2) & Tomomi YAMAZAKI2) 1) Department of Pediatrics, Chiba University School off Medicine 2) Department of Pathobiology, Chiba University School of Nursing 3) Department of Pethobiology, University of Washington We examined the cross reaction of Chlamydia trachomatis (C. trachomatis) by antigen detection method using EIA against the aerobic bacteria which colonize in the respiratory tract of children. Chlamydiazyme showed a cross reaction among 5 out of 7 species of Gram negative bacteria (=71.4%) and Neisseria and Branhamella catarrhalis showed a cross reaction even in low concentrations of 1 ~ CFU/ml. There were no Gram positive bactria which showed cross reaction. IDEIA Mark III did not react to Gram negative bacteria even in high concentratins of 1 ~ CFU/ml. One strain of coagulase positive staphylococcus (CPS) showed positive at a concentration of 1 ~ 108: however, other Gram positive bacteria including two other strains of CPS exhibited no cross reaction. The detectable concentration of C. trachomatis (D/UW 3 strain) using IDEIA Mark III was 1 ~ 103 IFU/ml. Low cross reaction rate and sensitivity suggest IDEIA Mark III is preferable for iagnosis of respiratory infection of C. trachomatis though further clinical studies are necessary.

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