- PDF Free Download

Size: px

Start display at page:

Download ""

みいかつなかわ
5 years ago
Views:

1 Powered by TCPDF ( Title Sub Title Author ヒト着床における子宮内膜上皮細胞の運動動態の解明と新規着床支援療法の確立 Analysis of human endometrial epithelial cell dynamics during implantation 内田, 浩 (Uchida, Hiroshi) 丸山, 哲夫 (Maruyama, Tetsuo) 升田, 博隆 (Masuda, Hirotaka) Publisher Publication year 2015 Jtitle 科学研究費補助金研究成果報告書 (2014. ) Abstract ヒト着床の分子機序解明は, 生殖補助医療の最大の障害となっている着床ロスの軽減に向けて渇望されている従来, 胚が子宮内膜の間質に陥入し妊娠が成立するには, 通過すべき子宮内膜上皮細胞層が細胞死を引き起こすことで胚の陥入ルートを形成するとされてきた本研究によって, 着床期の子宮内膜上皮細胞層の破壊は細胞死によるだけでなく, 子宮内膜上皮細胞の特異な遠心方向の運動によって構成されていることが明らかとなった Knowledge of human implantation mechanism is essential for assisted reproductive technology. It is known that human endometrial epithelial cells fall into apoptosis and disappear for embryo passing through. This study demonstrated that a number of human endometrial epithelial cells efferent move away from the center of embryo attachment, in association with cell apoptosis of small number of endometrial epithelial cells during implantation in vitro. Notes 研究種目 : 基盤研究 (C) 研究期間 : 2012~2014 課題番号 : 研究分野 : 生殖医学 Genre Research Paper URL

3 Glycodelin EMT; Epithelio-Mesenchymal Transition (Endocrinology 2005, 2007, Hum Reprod 2007, J Biol Chem 2012) ( C 16 17, 18 19, 21~23 ) Ishikawa JAR spheroid in vitro (1) in vitro Keyence BIOREVO BZ-9000in vitro Ishiakwa JAR spheroid 24 (2) (1) (3) small GTPase Rho family(rho, Rac, Cdc42)EMT N-cadherin (1) in vitro = in vitro JAR spheroid in vitro (2) in vitro Ishikawa thesc Ishikawa thesc HEC1B Ishikawa

4 HeLa CHO (3) Rho family small GTPase EMT N-cadherin *corresponding author Miyazaki K, Miki F, Uchida S, Masuda H, Uchida H, Maruyama T*: Serum estradiol level during withdrawal bleeding as a predictive factor for intermittent ovarian function in women with primary ovarian insufficiency. Endocr J. 2015; 62(1): DOI: /endocrj.EJ Uchida S, Uchida H, Maruyama T*, Kajitani T, Oda H, Miyazaki K, Kagami M, Yoshimura Y: Molecular analysis of a mutated FSH receptor detected in a patient with spontaneous ovarian hyperstimulation syndrome. PLoS One. 2013; 8(9): e DOI: /journal.pone Kajitani T, Maruyama T*, Asada H, Uchida H, Oda H, Nishikawa-Uchida S, Miyazaki K, Arase T, Ono M, Yoshimura Y: Possible involvement of nerve growth factor in dysmenorrhea and dyspareunia associated with endometriosis. Endocr J. 2013; 60(10): DOI: /endocrj.EJ Maruyama T*, Miyazaki K, Uchida H, Uchida S, Masuda H, Yoshimura Y: Achievement of pregnancies in women with primary ovarian insufficiency using close monitoring of follicle development: case reports. Endocr J. 2013; 60(6): DOI: /endocrj.EJ Maruyama T*, Miyazaki K, Masuda H, Ono M, Uchida H, Yoshimura Y:Human uterine stem/ progenitor cells: Implications for uterine physiology and pathology.placenta. 2013; 27: S68-S72.. DOI: /j.placenta Miyazaki K, Maruyama T*, Masuda H, Yamasaki A, Uchida S, Oda H, Uchida H, Yoshimura Y: Stem cell-like differentiation potentials of endometrial side population cells as revealed by a newly developed in vivo endometrial stem cell assay. PLoS One. 2012; 7(12): e DOI: /journal.pone Kagami M, Maruyama T*, Koizumi T, Miyazaki K, Nishikawa-Uchida S, Oda H, Uchida H, Fujisawa D, Ozawa N, Schmidt L, Yoshimura Y: Psychological adjustment and psychosocial stress among Japanese couples with a history of recurrent pregnancy loss. Hum Reprod. 2012; 27(3): DOI: /humrep/der441. Uchida H*, Maruyama T, Nishikawa-Uchida S, Oda H, Miyazaki K, Yamasaki A, Yoshimura Y: Studies using an in vitro model show evidence of involvement of epithelial-mesenchymal transition of human endometrial epithelial cells in human embryo implantation. J Biol Chem. 2012; 287(7): DOI: /jbc.M Kaoru Miyazaki, Yuki Ueno, Hirotaka Masuda, Hiroshi Uchida, Tetsuo Maruyama: Decellularization of the rat uterus yielded scaffold capable of tissue regeneration without immune rejection upon allotransplantation. 62nd Society for

5 Reproductive Investigation (SRI). March 25-28, 2015, San Francisco, USA. Hiroshi Uchida: Human endometrial epithelial cells in motion during implantation. Embryo Impantation and Pregnancy: Intricacies and strategies for its Success. March 9-11, 2015, New Delhi, India. Kaoru Miyazaki, Tetsuo Maruyama, Hirotaka Masuda, Naoko Hida, Hiroshi Uchida, Yasunori Yoshimura: Reconstruction and regeneration of the rat uterus through novel decellularization and recellularization techniques. 12nd International Society for Stem Cell Research (ISSCR). June 18-21, 2014, Vancouver, Canada., 2014,

Powered by TCPDF ( Title 明治以前日本水害史年表 Sub Title A chronological table of flood disasters before Meiji era in Japan Author 高木, 勇夫 (Takagi,

Powered by TCPDF ( Title 明治以前日本水害史年表 Sub Title A chronological table of flood disasters before Meiji era in Japan Author 高木, 勇夫 (Takagi, Powered by TCPDF (www.tcpdf.org) Title 明治以前日本水害史年表 Sub Title A chronological table of flood disasters before Meiji era in Japan Author 高木, 勇夫 (Takagi, Isao) Publisher 慶應義塾大学日吉紀要刊行委員会 Publication year 004