Fig. 1 Fishing Locations in Tokyo Bay, Sagami Bay and the Choshi Sea.

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1 Mercury Concentration of Fish in Tokyo Bay and the Surrounding Sea Area Ruijun ZHANG, Yuji KASHIMA, Mitsuaki MATSUI, Toshiko OKABE and Rikuo DOI Department of Hygiene, Yokohama City University School of Medicine, Yokohama Abstract Total mercury in the muscles of three fish species was analyzed in fish caught in Tokyo Bay and the surrounding sea areas, Sagami Bay and Choshi. Tokyo Bay is a semi-closed sea area surrounded by Tokyo, Kanagawa and Chiba prefectures. Sagami Bay and Choshi are open to the Pacific Ocean. A total of 412 fish consisting of northern whiting (Sillago japonica), flatfish (Limanda yokohamae) and sardine (Sardinops melanosticta) were caught in these areas over a 6 months period from November 1998 to April mercury concentrations and body length or body weight in northern whiting and flatfish, irrespective of the sea area. A correlation was also found between mercury concentration in fish and their feeding habits: among the 3 species caught in the same area, crustacean feeding northern whiting had the highest, polychaete feeding flatfish moderate, and plankton feeding sardine had the lowest mercury concentration. In a comparison of mercury concentration in the same species caught in different sea areas, a higher concentration was noted in fish caught in the semi-closed sea area of Tokyo Bay, than in fish caught in the open sea areas of Sagami Bay and Choshi. This difference was most marked in fish caught at the bottom of Tokyo Bay and we considered that the mercury concentration of seawater and sediment in these areas was the cause of mercury accumulation in fish. These findings suggest that improved water quality control and environmental monitoring is necessary in semi-closed sea areas such as Tokyo Bay. Reprint requests to: Ruijun ZHANG Department of Hygiene, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, , Japan TEL: +81(45) , FAX: +81(45)

2 Fig. 1 Fishing Locations in Tokyo Bay, Sagami Bay and the Choshi Sea.

3 Table 1 Mean Body Weight, Length, and Range, Median and SD of Total Hg Concentration Table 2 Fish Body Length-Hg Concentration Regression Equation, R and Probability

4 Fig. 2 Body Length-Total Hg Concentration Scatter Diagram of Fish Species.

5 Fig. 3 Body Length-Total Hg Concentration Scatter Diagram of Fisheries.

6 Table 4 Principal Ingredient Point and Order of Fish Hg Level of Fishery Fig. 4 Cluster Dendrogram of Fishing Locations.

7 1) Francesconi AK, Lenanton RCJ. Mercury Contamination in a Semi-enclosed Marine Embayment: Organic and Inorganic Mercury Content of Biota, and Factors Influencing Mercury Levels in Fish. Marine Environmental Research 1992; 33: ) Irie T. Bioaccumulation of mercury by marine Fish. Proceedings Japanese-French Workshop on Recent Progress on Knowledge of the Behaviour of Contaminants in Sediments and Their Toxicity to Aquatic Organisms. National Research Institute of Fisheries Science 1994: ) Kannan K, Smith RG, Jr, Lee RF, Windom HL, Heitmuller PT, Macauley JM, Summers JK. Distribution of total mercury and methylmercury in water, sedoment, and fish from South

8 Florida Estuaries. Archives of Environmental Contamination and Toxicology 1998; 34: ) Joins CR, Ali IB, Holsbeek L, Bossicart M, Tapia G. Total and organic mercury in Barents Sea pelagic fish. Bulletin of Environmental Contamination and Toxicology 1995; 5: ) Akagi Hirokatsu, Hajime Nishimura. Speciation of mercury in the environment. In: T. Suzuki, editor. Advances in Mercury Toxicology. New York: Plenum Press 1991: ) Pellegrini D, Barghigiani C. Feeding behaviour and mercury concentration in two flatfish in the North Tyrrhenian Sea. Mar Pollut Bull 1989; 20: ) Kehrig HA, Maim O, Moreira I. Mercury in a widely consumed fish micropogonias furnieri (Demarest, 1823) from four main Brazolian Estuaries. The Science of the Total Environment 1998; 213: ) Joins CR, Ali IB, Holsbeek L, Kanuya-Kinoti M, Tekele- Michael Y. Total and organic mercury in Greenland and Barents seas demersal Fish. Bulletin of Environmental Contamination and Toxicology 1997; 58: ) Leah Richard, Stephen Evans and Michael Johnson. Mercury in muscle tissue of lesser-spotted dogfish (scyliorhinus caniculus L.) from the north-east Irish sea. The Science of the Total Environment 1991; 108: ) Environmental Health Criteria 86 Mercury. WHO Geneva ) Gerhardsson L, Oslarsson A, Skerfving S. Acid precipitation effects on trace elements and human health. The Science of the Total Environment 1994; 153 (3): ) Murata K, Weihe P, Araki S, Budtz-Jorgensen E, Grandjean P. Evoked potentials in Faroese children prenatally exposed to methylmercury. Neurotoxicol Teratol 1999; 21 (4): ) Svensson BG, Nilsson A, Jonsson E, Schutz A, Akesson B, Hagmar L. Fish consumption and exposure to persistent organochlorine compounds, mercury, selenium and methylamines among Swedish fishermen. Scand J Work Environment Health 1995; 21 (2): ) Fitzgerald WF, Clarkson TW. Mercury and monomethylmercury: present and future concerns. Environment Health Perspect 1991; 96: ) Weihe P, Grandjean P, Debes F, White R. Health implications for Faroe islanders of heavy metals and PCBs from pilot whales. The Science of the Total Environment 1996; 186 (1-2):

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