Table 1. Influence of food deprivation on gastric secretion and severity of ulceration in 18 hr pylorus ligated rats.

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2 Table 1. Influence of food deprivation on gastric secretion and severity of ulceration in 18 hr pylorus ligated rats.

3 Fig. 1. Effects of repeated administrations of several mild antiulcer agents on severity of ulceration. Drugs were administered orally twice a day for two days during food deprivation and intraduodenally after pyloric ligation. Figures in parenthesis indicate numbers of rats. Statistically significant at p=0.05(*) and p=0.01(**). Table 2. Effects of repeated administrations of MMSCI and chlorophyll on gastric secretion. Drugs were administered orally twice a day for two days during food deprivation and intraduod enally immediately after pyloric ligation. Statistically significant at p=0.05(*) and p=0.01(**).

4 Fig. 2. Effects : of -repeated interperitoneal administrations of MMSCI on severity of ulceration and gastric secretion. Each group included 6 animals. MMSCI was administered i.p. twice a day for two days during food deprivation and immediately after pyloric ligation. Table 3. Effects of a single administration of MMSCI and chlorophyll on gastric secretion and severity of ulceration. Drugs were administered orally 6 hr before pyloric ligation or intraduodenally immediately after pyloric ligation. Statistically significant at p=0.05(*) and p=0.01(**).

5 Fig. 3. Synergistic action between repeated administrations of MMSCI and a single administration of several anti-ulcer agents on severity of ulceration. MMSCI (1 g/kg) was administered orally twice a day for two days during food deprivation and intraduodenally after pyloric ligation. Other drugs were administered immediately after pyloric ligation in the following doses : atro pine 0.5 mg/kg, s.c., antacids 500 mg/kg, p.o., APS 50 mg/kg, p.o. and Fa mg/kg, i.p. Table 4. Effects of combinations between MMSCI and other anti-ulcer agents on gastric secretion. Schedule for drug administration is given in the legend of Fig. 3. Statistically significant at p=0.05(*) and p=0.01(**).

6 1) SHAY, H., KOMAROV, S. A., FELS, S. S., MERANCE, D., GRUENSTEIN, M. and SIPLET, H.: Gastro enterology 5, 43 (1945) 2) SHAY, H., GRUJENSTEIN, M., SIPLET, H. and KOMAROV, S. A.: Proc. Soc. exp. Biol. Med. 69, 369 (1948) 3) ISHII, Y : Japan. J. Pharmacol. 19, 125 (1969) 6) OKABE, S., OHTU, K., TAKEUCHI, K. and TAKAGI, K.: Japan. J. Pharmacol. 24, 169 (1974) 7) ANSON, M. L. : J. Gen. Physiol. 22, 79 (1938) 8) SZABO, Zs. and VARGHA, G. : Arzneim. Forsch. 10, 23 (1960) 9) NAMEKATA, M.: Chem. Pharm. Bull. 10, 177 (1962)

7 10) TAKAGI, K., KASUYA, Y. and WATANABE, K.: Chem. Pharm. Bull. 11, 1282 (1963) 11) BRODIE, D. A. : Gastroenterology 50, 45 (1966) 12) BIANCHI, R. G. and COOK, D. L. : Gastroenterology 47, 409 (1964) 14) KOKUE, E., HAYAMA, T. and NAKAMURA, T.: Japan. J. Pharmacol. 24, 621 (1974) 15) TAKAGI, K. and ISHII, Y.: Arzneim. Forsch. 17, 1544 (1967) 16) ISHII, Y. : Arzneim. Forsch. 18, 53 (1968) 18) TAKAGI, K., OKABE, S. and SAZIKI, R.: Japan. J. Pharmacol. 19, 418 (1969) ) TAKAGI, K. and YANO, S.: Chem. Pharm. Bull. 20, 1170 (1972) 19 Abstract-Yasuo ISHII and Yuichi FUJIIResearch Laboratories, Nippon Kayaku Co., Shimo, Kita ku, Tokyo 115, Japan). Effects of several mild anti-ulcer agents on pylorus ligated rats (Shay rats).folia pharmacol. japon. 70, (1974). The inhibitory action of several mild anti-ulcer agents on gastric ulcer in the pylorus ligated rats (Shay rats) and availability of this method were investigated. Food deprivation and ligation times were set at 48 hr and 18 hr, respectively. Drugs were administered orally twice a day during food deprivation and intraduodenally immediately after pyloric ligation. Glutamine, Fu 100 (a fraction from licorice root extract) and glucose showed no inhibitory action on ulceration. Methylmethion ine sulfonium chloride (MMSCl, 1 or 2 g/kg ~ 5) and chlorophyll (1 g/kg ~ 5) inhibited ulceration dose-dependently though the doses were extremely high compared with clinical ones. A single ad ministration of MMSC1 or chlorophyll immediately after pyloric ligation showed no inhibitory action. Synergistic action between repeated administration of MMSCl and a single administration of atropine, antacids, amylopectin sulfate or FM100 was observed when using this method. Shay rats may therefore be useful for evalution of mild anti-ulcer agents as well as anticholinergic agents, pepsin inhibitors and antacids.

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