小児感染免疫第20巻第3号

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1 28 Vol. 2No purified pertussis vaccine WHO WHO Bordetella pertussis m 45 1 mm S R K 2 2 ml Basic research and development of purified pertussis vaccine in Japan, and the vaccine thereafter

2 48 28 Medical Research CouncilMRC Dr. Kendrick MRC 2 2 IgG 195 Dr. Pillmer MRC O 1 StromaAdsorbed Protective Antigens SAPA MRC SAPA SAPA SAPA SAPA pertussis toxinptsapa PT MRC Ph. D National Insti-

3 28 Vol. 2No. 49 tute of HealthNIH GHQ FDA Bureau of Biologics National Institute of Infectious Diseases m 2 2 ml ml , 7, numbering sample

4 Lots 2 2 ml ml 5 14 Kendrick test WHO ml PT CVI WHO2 Award

5 28 Vol. 2No P 2 2 DP 2 DPT 4 DPT DEAECM Dr. Ribi cellfractionator 22S 1967 Ribi cellfractionator HSF 1967 Dr. Pittman NIH information histaminesensitizing factorhsf

6 I LPF 1 nm Pittman lymphocytosispromoting factorlpf HSF LPF Infection and Immunity 52 5 ml.2 ml 15, g 4,pmmin 152 LPF origin LP HShemagglutininHAislet activatingia 2 2 HA 2 HA 2 HA LPFHAPT S1S2S2S4 S5 F HAfilamentoushemagglutinin mm 2 HA T DPT

7 28 Vol. 2No. 5 2 P. DP. DPT A 2 1 soluble form LPFHA FHA 5 cellwall vaccineribosomal vaccinedetergenttreated vaccine LPFHA FHA HA 1 6 HA HA HA 1, ml 5 95 ml

8 ml 2,. p.m. 18 ml 2 No HA OD 28 HA 1224 HA ml 2 LPFHA 2 FHA 22 LPFHA 15gkg pool HA.5 1 M Nacl P. B. 7 LPFHAPT LPFHA IgG.2 mgml LPFHA FHA 8 ml 1 l 1 l, l6 dose T S HA B T 11 B T 1 HA 1979 FDABOB Dr. Pittman Pittman guest worker Dr. M

9 28 Vol. 2No Smith Kline Beecham Smith Kline Connaught Connaught Amvax JapanNIH6Biken JapanNIH7Biken LederleTakeda Lederle Smith KlineBeecham Smith KlineBeecham Biken Behringwerke ChironBiocine SmithKline Beecham Connought PasteurMerieux PasteurMerieux Sweden Sweden Sweden Sweden Sweden Sweden Germany Germany Germany Germany Germany Germany Italy Italy Italy Senegal Senegal DTaP2 1 DTaP5 DTwP DTaP1 ap2 ap1 DTaP4 DTwP DTaP DTwP DTaP2 DTwP DTaP DTaP DTwP DTaP2 DTwP g Proteindose PT FHA PRN Aggs CI DTaP2 Diphthexia toxoidtetanus toxoid2 Pertussis PT FHA WPWhole cell Pertussis vaccine, P1PT toxoid P2P1FHA, PP2Pertuctin, P4P Agglutinogen 2, P5P4Agglutinogen 2 CRM PT HA ELISA HA PT PT WHO WHO PT FHA PT FHA 2g dose 2 JapanNIH6 PT 8gdose JapanNIH FHA placebo control 1 adverse reaction 1987

10 JapanNIH PT FHA pertactin PRNAgg2 Agg PRNAggs PTPTFHAPTFAHPRN PTFHAPRNAggs 4 CRM 1992 FDA T B WHO JNIH6 JNIH Children s Vaccine Initiative CVI11 1 WHO Drs. Yuji SATO & Hiroko SATO WHO WHO dose dose dose 45 dose modify 28 5 Dr. KeithNIH, USA 1987 Yang Xiao Ming Dr. Yang C l1 5

11 28 Vol. 2No. 57 6, dose Sato Y, Arai HLeukocytosispromoting factor of Bordetella pertussis. I. Purification and characterization. Infect Immun , Sato Y, Arai H, Suzuki KLeukocytosispromoting factor of Bordetella pertussis.. Biological properties. Infect Immun , 197 Sato Y, Arai, H, Suzuki KLeukocytosispromoting factor of Bordetella pertussis.. Its identity with protective antigen. Infect Immun , Arai H, Sato YSeparation and characterization of two distinct hemagglutinins contained in purified leukocytosispromoting factor from Bordetella pertussis. Biochem Biophys Acta , Sato Y, Izumiya K, Sato H, et alaerosol infection of mice with Bordetella pertussis. Infect Immun , 198 6Sato Y, Izumiya K, Sato H, et alrole of antibody to Leukocytosispromoting factor hemagglutinin and to filamentous hemagglutinin in immunity to pertussis. Infect Immun , Sato Y, Cowell JL, Sato H, et alseparation and purification of the hemagglutinins from Bordetella pertussis. Infect Immun 4112, 198 8Sato H, Sato Y, Ito IAffinity of pertussis toxin produced by Bordetella pertussis for human haptoglobinapplication to the in vitro assay of the toxin. J Microbiol Method 19919, 198 9Imaizumi A, Suzuki Y, Ono S, et aleffect of heptakis2,6odimethylbcyclodextrin on the production of pertussis toxin by Bordetella pertussis. Infect Immun , 198 1Oda M, Izumiya K, Sato YTransplacental and transcolostral immunity to pertussis in a mouse model using acellular pertussis vaccine. J Infect Disease , Sato Y, Kimura M, Fukumi HDevelopment of a pertussis component vaccine in Japan. Lancet , Sato H, Sato YBordetella pertussis infection in micecorrelation of specific antibodies against two antigens, pertussis toxin, and filamentous hemagglutinin with mouse protectivity in an intracerebral or aerosol challenge system. Infect Immun , Sato H, Ito H, Chiba J, et almonoclonal antibody against pertussis toxineffect on toxin activity and pertussis infection. Infect Immun , Sato Y, Sato HAnti pertussis toxin IgG and anti filamentous hemagglutinin IgG production in children immunized with pertussis acellular vaccine and comparison of these titers with the sera of pertussis convalescent children. Develop Biol Standard , Sato H, Sato YProtective antigens of Bordetella pertussismouseprotection test against intracerebral and aerosol challenge of B. pertussis. Develop

12 58 28 Biol Standard , Isomura S, Suzuki S, Sato YClinical efficacy of the Japanese acellular pertussis vaccine after intrafamiliar exposure to pertussis patients. Develop Biol Standard , Sato H, Sato YMonoclonal antibody against pertussis toxinrelationship between toxin neutralization and mouse protection activities. Ann Sclavo n , Sato YUse of the intracerebral challenge, mouse potency assay to standardize and license the Japanese acellular pertussis vaccine. Workshop on acellular pertussis vaccine. Department of Health and Human Service. Bethesda, Maryland, September, Sato H, Sato Y, Ito I, et aleffect of monoclonal antibody to pertussis toxin on toxin activity. Infect Immun , Sato Y, Sato H, Ito I, et alisolation of mutant strains producing defective pertussis toxin, Japan. J Med Sci Biol , Locht C, Cieplak W, Marchitto K, et alactivities of complete and truncated forms o pertussis toxin subunits S1 and S2 expressed in Escherichia coli. Infect Immun 55465, Placebocontrolled trial of two acellular pertussis vaccines in Sweden. Protective efficacy and adverse events. Lancet i955, 96, Sato H, Sato YAn attempt to introduce encepharopathy with pertussis toxin in mice. Japan. J Med Sci Biol 4126, Sato H, Chiba J, Sato YMonoclonal antibodies against alpha toxin of Clostridium perfringes. FEMS Microbiol Letters , 1989 Yamakawa Y, Sato H, Sato YIsolation of pertussis toxin subunit proteins by reversephase high performance liquid chromatography and reconstitution of the holotoxin molecule. Analytical Biochem , Sato H, Sato YProtective activities in mice of monoclonal antibodies against pertussis toxin. Infect Immun , Sato H, Sato YRelationship between mouseprotecting and toxinneutralizing activities of anti pertussis toxin monoclonal antibodies, Japan. J Med Sci Biol , Sato H, Sato YRelationship between structure and biological and protective activities of pertussis toxin. Dev Biol Stand , Sato Y, Sato HCharacterization of mutant strains producing pertussis toxin CRMs. Dev Biol Stand 7917, 1991 Brown DR, Keith JM, Sato H, et alconstruction and characterization of genetically inactivated pertussis toxin. Dev Biol Stand 767, Matsuura Y, Sato H, Sato YExpression of the S1 subunit of pertussis toxin using a recombinant baculovirus. Dev Biol Stand 77985, Sato Y, Sato HComparison of toxicities of acellular pertussis vaccine with whole cell pertussis vaccine in experimental animals. Dev Biol Stand , 1991 Sato Y, Sato H, Kodama H, et alan improved ELISA system for the measurement of IgG antibodies against pertussis toxinptand filamentous hemagglutinin FHA in human sera. Dev Biol Stand , Sato H, Sato Y, Ohishi IComparison of pertussis toxinptneutralizing activities and mouseprotective activities of antipt mouse monoclonal antibodies. Infect Immun , Sato H, Sato YRelationship between mouseprotective and toxinneutralising activities of anti pertussis toxin monoclonal antibodies. J Med Microbiol , Sato Y, Sato HAcellular pertussis vaccine as a solution to the prevention of whooping cough. Current Opinion in Infectious Diseases

Key words : Adverse reactions, Egg allergy, IgG antibody, Mills allergy, FAST

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